A transposon-mediate inactivation of a CYCLOIDEA-like gene originates polysymmetric and androgynous ray flowers in Helianthus annuus
Genetica
Marco Fambrini, Mariangela Salvini, Claudio Pugliesi
Correspondence:
Pugliesi Claudio, Dipartimento di Biologia delle Piante Agrarie, Sezione di Genetica Agraria, Università di Pisa, Via Matteotti 1B, I-56124 Pisa, Italy.
E-mail:
Supplemental Fig. 7Nucleotide sequence alignments of the CYC10/CYC11 (a) and the RAG19/CYC11 (b) PCR amplified products from wild type (TURF), tubular ray flower mutant (turf) of sunflower (Helianthus annuus L.) andindividuals of a progenyobtained from a self-fertilized revertant. a Nucleotide sequences of homozygous (TT)and heterozygous (Tt) individuals of a progeny obtained from a self-fertilized revertant and TURF. bNucleotide sequences of heterozygous (Tt) and homozygous (tt) individuals of a progeny obtained froma self-fertilized revertantand turf. Underlined in bold is a portion of the CACTA transposable element (Tetu1). The three bp (ATA)target site duplication (TSD) are in white letters and highlighted in black. Highlighted in yellow and grey are the primers CYC10 and CYC11 used in a, respectively. Highlighted in light-blue and grey are the primers RAG19 and CYC11 used in b,respectively. The arrows indicate the 5’-3’-primer direction. The start and the stop codons are in bold, underlined and highlighted in green and magenta, respectively. The intron, located in the 3’-UTR region is in italic and underlined. Asterisks indicate identical nucleotides.
a
TURF CAGTGTAAAGCTTAAGAGGGGTGGTGGTCTCTTAAATGCAACTTTACCCTTAGCTTTATT 60
TT CAGTGTAAAGCTTAAGAGGGGTGGTGGTCTCTTAAATGCAACTTTACCCTTAGCTTTATT 60
Tt CAGTGTAAAGCTTAAGAGGGGTGGTGGTCTCTTAAATGCAACTTTACCCTTAGCTTTATT 60
************************************************************
TURF TGGATCAATGAACTTATATCTAAACTTCTTTGTTAACTATTATTGTTATTGTTTCTTTCA 120
TT TGGATCAATGAACTTATATCTAAACTTCTTTGTTAACTATTATTGTTATTGTTTCTTTCA 120
Tt TGGATCAATGAACTTATATCTAAACTTCTTTGTTAACTATTATTGTTATTGTTTCTTTCA 120
************************************************************
TURF TTTCCAAATTACAATGTTTTCCTCAAACCCTTTTCCACAGATTCCATCTCCCATCCATGT 180
TT TTTCCAAATTACAATGTTTTCCTCAAACCCTTTTCCACAGATTCCATCTCCCATCCATGT 180
Tt TTTCCAAATTACAATGTTTTCCTCAAACCCTTTTCCACAGATTCCATCTCCCATCCATGT 180
************************************************************
TURF CTCACATCCTTTCAATTCCTTCTTTGACCTTGAAAGAAACGATGTATATTTCAACCATCA 240
TT CTCACATCCTTTCAATTCCTTCTTTGACCTTGAAAGAAACGATGTATATTTCAACCATCA 240
Tt CTCACATCCTTTCAATTCCTTCTTTGACCTTGAAAGAAACGATGTATATTTCAACCATCA 240
************************************************************
TURF TGAAGACAACAATAACCCTTTTGTTTCCGGTGATTCTTTTTTACATTCCTACAGCAGTTT 300
TT TGAAGACAACAATAACCCTTTTGTTTCCGGTGATTCTTTTTTACATTCCTACAGCAGTTT 300
Tt TGAAGACAACAATAACCCTTTTGTTTCCGGTGATTCTTTTTTACATTCCTACAGCAGTTT 300
************************************************************
TURF TGCTCCACAACCATCAACCCTACCTCCAGTCACAGACTATATACCTTCAAATGCACAAGA 360
TT TGCTCCACAACCATCAACCCTACCTCCAGTCACAGACTATATACCTTCAAATGCACAAGA 360
Tt TGCTCCACAACCATCAACCCTACCTCCAGTCACAGACTATATACCTTCAAATGCACAAGA 360
************************************************************
TURF GTTAGATAGTCAAAACCAGCTGCTTGATCATGAAGGGTCTGGGTTGCAATATTGTGACAA 420
TT GTTAGATAGTCAAAACCAGCTGCTTGATCATGAAGGGTCTGGGTTGCAATATTGTGACAA 420
Tt GTTAGATAGTCAAAACCAGCTGCTTGATCATGAAGGGTCTGGGTTGCAATATTGTGACAA 420
************************************************************
TURF TTATAGTGATCTTTTGGAGTCAGTAGTTTACCCATCAAAGAAAAAAGTGGCGATTTCAAA 480
TT TTATAGTGATCTTTTGGAGTCAGTAGTTTACCCATCAAAGAAAAAAGTGGCGATTTCAAA 480
Tt TTATAGTGATCTTTTGGAGTCAGTAGTTTACCCATCAAAGAAAAAAGTGGCGATTTCAAA 480
************************************************************
TURF AAAAGATGGGCATAGTAAAATCTATACCGCACAAGGCCCGAGAGATCGGAGGGTGAGATT 540
TT AAAAGATGGGCATAGTAAAATCTATACCGCACAAGGCCCGAGAGATCGGAGGGTGAGATT 540
Tt AAAAGATGGGCATAGTAAAATCTATACCGCACAAGGCCCGAGAGATCGGAGGGTGAGATT 540
************************************************************
TURF GTCCATTGACATTGCAAGAAAGTTTTTTGTGCTTCAAGATTTGCTAGGTTTTGACAAAGC 600
TT GTCCATTGACATTGCAAGAAAGTTTTTTGTGCTTCAAGATTTGCTAGGTTTTGACAAAGC 600
Tt GTCCATTGACATTGCAAGAAAGTTTTTTGTGCTTCAAGATTTGCTAGGTTTTGACAAAGC 600
************************************************************
TURF AAGCAAAACCCTTGATTGGCTCTTTACCAAGTCCAAGAAGGCGATTAAGGAGTTGATTGA 660
TT AAGCAAAACCCTTGATTGGCTCTTTACCAAGTCCAAGAAGGCGATTAAGGAGTTGATTGA 660
Tt AAGCAAAACCCTTGATTGGCTCTTTACCAAGTCCAAGAAGGCGATTAAGGAGTTGATTGA 660
************************************************************
TURF AGAAACAAAGCACTCCTCATCTTCCACTGTTAGTACTAATCAGTGTGAAATGGCTTTCCT 720
TT AGAAACAAAGCACTCCTCATCTTCCACTGTTAGTACTAATCAGTGTGAAATGGCTTTCCT 720
Tt AGAAACAAAGCACTCCTCATCTTCCACTGTTAGTACTAATCAGTGTGAAATGGCTTTCCT 720
************************************************************
TURF AGAGACCATCAAGGGAGGATCAGGATCAGATGAAGATAAAGGGCAAAAAATGTCAGCTCT 780
TT AGAGACCATCAAGGGAGGATCAGGATCAGATGAAGATAAAGGGCAAAAAATGTCAGCTCT 780
Tt AGAGACCATCAAGGGAGGATCAGGATCAGATGAAGATAAAGGGCAAAAAATGTCAGCTCT 780
************************************************************
TURF CAGGTTTCTTGATGGAGAAAAGAAGAAAATGACACAAAAATGCAAATCTGGATTTCATGC 840
TT CAGGTTTCTTGATGGAGAAAAGAAGAAAATGACACAAAAATGCAAATCTGGATTTCATGC 840
Tt CAGGTTTCTTGATGGAGAAAAGAAGAAAATGACACAAAAATGCAAATCTGGATTTCATGC 840
************************************************************
TURF TAGTCTTGCAAGAGGCCAGTCAAGGGCAGAGGCAAGAGCAAGGGCTAGGGAGAGAACAAA 900
TT TAGTCTTGCAAGAGGCCAGTCAAGGGCAGAGGCAAGAGCAAGGGCTAGGGAGAGAACAAA 900
Tt TAGTCTTGCAAGAGGCCAGTCAAGGGCAGAGGCAAGAGCAAGGGCTAGGGAGAGAACAAA 900
************************************************************
TURF ACAAAAATTGCGCATCAAGGAGCTAGACAACGACTTGAAGAAAATTCCTGATGATTACCC 960
TT ACAAAAATTGCGCATCAAGGAGCTAGACAACGACTTGAAGAAAATTCCTGATGATTACCC 960
Tt ACAAAAATTGCGCATCAAGGAGCTAGACAACGACTTGAAGAAAATTCCTGATGATTACCC 960
************************************************************
TURF TTGTCATGCATTAAGCCCCTCAAACACAACACTCCAATCAAACTCTTGGGGTCAATTTGA 1020
TT TTGTCATGCATTAAGCCCCTCAAACACAACACTCCAATCAAACTCTTGGGGTCAATTTGA 1020
Tt TTGTCATGCATTAAGCCCCTCAAACACAACACTCCAATCAAACTCTTGGGGTCAATTTGA 1020
************************************************************
TURF ATCACAAAGTGACTATAATGACATCCTTCATGGATCAATGCTGGAACAAAGGTTTTCAGT 1080
TT ATCACAAAGTGACTATAATGACATCCTTCATGGATCAATGCTGGAACAAAGGTTTTCAGT 1080
Tt ATCACAAAGTGACTATAATGACATCCTTCATGGATCAATGCTGGAACAAAGGTTTTCAGT 1080
************************************************************
TURF ATCATCTTCAACTTTGTATACTTACAATCACAACTTTGTTGTATCAGATGAGTGCATCTC 1140
TT ATCATCTTCAACTTTGTATACTTACAATCACAACTTTGTTGTATCAGATGAGTGCATCTC 1140
Tt ATCATCTTCAACTTTGTATACTTACAATCACAACTTTGTTGTATCAGATGAGTGCATCTC 1140
************************************************************
TURF CCAAATTTCATCCAAAGGTATGCCTAAATTTAAAGTAATTCACGAGGAGCAGAGTCACCG 1200
TT CCAAATTTCATCCAAAGGTATGCCTAAATTTAAAGTAATTCACGAGGAGCAGAGTCACCG 1200
Tt CCAAATTTCATCCAAAGGTATGCCTAAATTTAAAGTAATTCACGAGGAGCAGAGTCACCG 1200
************************************************************
TURF TGCAATGATATAGATCTACAAAAGGTAGATTAATTAATCAAATACAAAACACGGTAGTTA 1260
TT TGCAATGATATAGATCTACAAAAGGTAGATTAATTAATCAAATACAAAACACGGTAGTTA 1260
Tt TGCAATGATATAGATCTACAAAAGGTAGATTAATTAATCAAATACAAAACACGGTAGTTA 1260
************************************************************
TURF CTGAATATAGTTTTTAAGATTTTATATGCCTTTGTTTTGTCAACAGAACTCTGAAAGGGG 1320
TT CTGAATATAGTTTTTAAGATTTTATATGCCTTTGTTTTGTCAACAGAACTCTGAAAGGGG 1320
Tt CTGAATATAGTTTTTAAGATTTTATATGCCTTTGTTTTGTCAACAGAACTCTGAAAGGGG 1320
************************************************************
TURF TGCTGGAGAAAATCTAGTCTTCATCAGCCGGCAAATAGATCTAGTAACCACTTCTCGGCA 1380
TT TGCTGGAGAAAATCTAGTCTTCATCAGCCGGCAAATAGATCTAGTAACCACTTCTCGGCA 1380
Tt TGCTGGAGAAAATCTAGTCTTCATCAGCCGGCAAATAGATCTAGTAACCACTTCTCGGCA 1380
************************************************************
TURF CTAGTTGATTGG 1392
TT CTAGTTGATTGG 1392
Tt CTAGTTGATTGG 1392
************
b
turf GGTTGCATTTGAGTGTAACAAAGGTTGCATTTTGGTGTAGAAAAGGTTGCGTTTGAGGTG 60
Tt GGTTGCATTTGAGTGTAACAAAGGTTGCATTTTGGTGTAGAAAAGGTTGCGTTTGAGGTG 60
tt GGTTGCATTTGAGTGTAACAAAGGTTGCATTTTGGTGTAGAAAAGGTTGCGTTTGAGGTG 60
************************************************************
turf GCAAAACTTGCATTTGAGATAACAAAGGTTACATTTGAGTGCTAGAAAAGTTGCACTGAA 120
Tt GCAAAACTTGCATTTGAGATAACAAAGGTTACATTTGAGTGCTAGAAAAGTTGCACTGAA 120
tt GCAAAACTTGCATTTGAGATAACAAAGGTTACATTTGAGTGCTAGAAAAGTTGCACTGAA 120
************************************************************
turf AGTAAAAAGAGTTGCGTTAGGATATAAAAAAGGTTGCATTATGAAAAGGTTGCATTTGAA 180
Tt AGTAAAAAGAGTTGCGTTAGGATATAAAAAAGGTTGCATTATGAAAAGGTTGCATTTGAA 180
tt AGTAAAAAGAGTTGCGTTAGGATATAAAAAAGGTTGCATTATGAAAAGGTTGCATTTGAA 180
************************************************************
turf TAGTAAATGCAACTCTTTAAAAAAAGGTTGCATTTTCTAACAAAAAGGTTGCATTAGGTC 240
Tt TAGTAAATGCAACTCTTTAAAAAAAGGTTGCATTTTCTAACAAAAAGGTTGCATTAGGTC 240
tt TAGTAAATGCAACTCTTTAAAAAAAGGTTGCATTTTCTAACAAAAAGGTTGCATTAGGTC 240
************************************************************
turf TAATGCAACTTCATCTAATGCAGCCCTTGATAAAAGGTTGCATTAGGCCTAATGCAACCT 300
Tt TAATGCAACTTCATCTAATGCAGCCCTTGATAAAAGGTTGCATTAGGCCTAATGCAACCT 300
tt TAATGCAACTTCATCTAATGCAGCCCTTGATAAAAGGTTGCATTAGGCCTAATGCAACCT 300
************************************************************
turf TTTTAACCCCTAATGCAACATTTTATAAGGGTTGCATTAGCATCATTTTCTTGTAGTGAT 360
Tt TTTTAACCCCTAATGCAACATTTTATAAGGGTTGCATTAGCATCATTTTCTTGTAGTGAT 360
tt TTTTAACCCCTAATGCAACATTTTATAAGGGTTGCATTAGCATCATTTTCTTGTAGTGAT 360
************************************************************
turf AGTAAAATCTATACCGCACAAGGCCCGAGAGATCGGAGGGTGAGATTGTCCATTGACATT 420
Tt AGTAAAATCTATACCGCACAAGGCCCGAGAGATCGGAGGGTGAGATTGTCCATTGACATT 420
tt AGTAAAATCTATACCGCACAAGGCCCGAGAGATCGGAGGGTGAGATTGTCCATTGACATT 420
************************************************************
turf GCAAGAAAGTTTTTTGTGCTTCAAGATTTGCTAGGTTTTGACAAAGCAAGCAAAACCCTT 480
Tt GCAAGAAAGTTTTTTGTGCTTCAAGATTTGCTAGGTTTTGACAAAGCAAGCAAAACCCTT 480
tt GCAAGAAAGTTTTTTGTGCTTCAAGATTTGCTAGGTTTTGACAAAGCAAGCAAAACCCTT 480
************************************************************
turf GATTGGCTCTTTACCAAGTCCAAGAAGGCGATTAAGGAGTTGATTGAAGAAACAAAGCAC 540
Tt GATTGGCTCTTTACCAAGTCCAAGAAGGCGATTAAGGAGTTGATTGAAGAAACAAAGCAC 540
tt GATTGGCTCTTTACCAAGTCCAAGAAGGCGATTAAGGAGTTGATTGAAGAAACAAAGCAC 540
************************************************************
turf TCCTCATCTTCCACTGTTAGTACTAATCAGTGTGAAATGGCTTTCCTAGAGACCATCAAG 600
Tt TCCTCATCTTCCACTGTTAGTACTAATCAGTGTGAAATGGCTTTCCTAGAGACCATCAAG 600
tt TCCTCATCTTCCACTGTTAGTACTAATCAGTGTGAAATGGCTTTCCTAGAGACCATCAAG 600
************************************************************
turf GGAGGATCAGGATCAGATGAAGATAAAGGGCAAAAAATGTCAGCTCTCAGGTTTCTTGAT 660
Tt GGAGGATCAGGATCAGATGAAGATAAAGGGCAAAAAATGTCAGCTCTCAGGTTTCTTGAT 660
tt GGAGGATCAGGATCAGATGAAGATAAAGGGCAAAAAATGTCAGCTCTCAGGTTTCTTGAT 660
************************************************************
turf GGAGAAAAGAAGAAAATGACACAAAAATGCAAATCTGGATTTCATGCTAGTCTTGCAAGA 720
Tt GGAGAAAAGAAGAAAATGACACAAAAATGCAAATCTGGATTTCATGCTAGTCTTGCAAGA 720
tt GGAGAAAAGAAGAAAATGACACAAAAATGCAAATCTGGATTTCATGCTAGTCTTGCAAGA 720
************************************************************
turf GGCCAGTCAAGGGCAGAGGCAAGAGCAAGGGCTAGGGAGAGAACAAAACAAAAATTGCGC 780
Tt GGCCAGTCAAGGGCAGAGGCAAGAGCAAGGGCTAGGGAGAGAACAAAACAAAAATTGCGC 780
tt GGCCAGTCAAGGGCAGAGGCAAGAGCAAGGGCTAGGGAGAGAACAAAACAAAAATTGCGC 780
************************************************************
turf ATCAAGGAGCTAGACAACGACTTGAAGAAAATTCCTGATGATTACCCTTGTCATGCATTA 840
Tt ATCAAGGAGCTAGACAACGACTTGAAGAAAATTCCTGATGATTACCCTTGTCATGCATTA 840
tt ATCAAGGAGCTAGACAACGACTTGAAGAAAATTCCTGATGATTACCCTTGTCATGCATTA 840
************************************************************
turf AGCCCCTCAAACACAACACTCCAATCAAACTCTTGGGGTCAATTTGAATCACAAAGTGAC 900
Tt AGCCCCTCAAACACAACACTCCAATCAAACTCTTGGGGTCAATTTGAATCACAAAGTGAC 900
tt AGCCCCTCAAACACAACACTCCAATCAAACTCTTGGGGTCAATTTGAATCACAAAGTGAC 900
************************************************************
turf TATAATGACATCCTTCATGGATCAATGCTGGAACAAAGGTTTTCAGTATCATCTTCAACT 960
Tt TATAATGACATCCTTCATGGATCAATGCTGGAACAAAGGTTTTCAGTATCATCTTCAACT 960
tt TATAATGACATCCTTCATGGATCAATGCTGGAACAAAGGTTTTCAGTATCATCTTCAACT 960
************************************************************
turf TTGTATACTTACAATCACAACTTTGTTGTATCAGATGAGTGCATCTCCCAAATTTCATCC 1020
Tt TTGTATACTTACAATCACAACTTTGTTGTATCAGATGAGTGCATCTCCCAAATTTCATCC 1020
tt TTGTATACTTACAATCACAACTTTGTTGTATCAGATGAGTGCATCTCCCAAATTTCATCC 1020
************************************************************
turf AAAGGTATGCCTAAATTTAAAGTAATTCACGAGGAGCAGAGTCACCGTGCAATGATATAG 1080
Tt AAAGGTATGCCTAAATTTAAAGTAATTCACGAGGAGCAGAGTCACCGTGCAATGATATAG 1080
tt AAAGGTATGCCTAAATTTAAAGTAATTCACGAGGAGCAGAGTCACCGTGCAATGATATAG 1080
************************************************************
turf ATCTACAAAAGGTAGATTAATTAATCAAATACAAAACACGGTAGTTACTGAATATAGTTT 1140
Tt ATCTACAAAAGGTAGATTAATTAATCAAATACAAAACACGGTAGTTACTGAATATAGTTT 1140
tt ATCTACAAAAGGTAGATTAATTAATCAAATACAAAACACGGTAGTTACTGAATATAGTTT 1140
************************************************************
turf TTAAGATTTTATATGCCTTTGTTTTGTCAACAGAACTCTGAAAGGGGTGCTGGAGAAAAT 1200
Tt TTAAGATTTTATATGCCTTTGTTTTGTCAACAGAACTCTGAAAGGGGTGCTGGAGAAAAT 1200
tt TTAAGATTTTATATGCCTTTGTTTTGTCAACAGAACTCTGAAAGGGGTGCTGGAGAAAAT 1200
************************************************************
turf CTAGTCTTCATCAGCCGGCAAATAGATCTAGTAACCACTTCTCGGCACTAGTTGATTGG 1259
Tt CTAGTCTTCATCAGCCGGCAAATAGATCTAGTAACCACTTCTCGGCACTAGTTGATTGG 1259
tt CTAGTCTTCATCAGCCGGCAAATAGATCTAGTAACCACTTCTCGGCACTAGTTGATTGG 1259
***********************************************************