Figure 1. Comparison of Upstream Genomic Region of T. Arvense CBF with Arabidopsis CBF1

Figure 1. Comparison of upstream genomic region of T. arvense CBF with Arabidopsis CBF1, CBF2, and CBF3. The number at the left indicates the position of the sequence upstream of the start codon. The background shading represents conserved nucleotides. The dashes indicate gaps introduced to maximize alignment. Some of the conserved potential promoter elements as identified with the PLACE database ( are indicated above and below the sequence. The probe containing the DRE/CRT element in the Ta-CBF promoter is highlighted in red.

LTRE1HVBL49

T.arvense -2595AAAACAAGTTTCCCAAAGTAGAGTATAAAACCGAAACAACC-GTTTCAGGCAAACCGCGA
CBF1 -1271 CACTGAAGAATCCCAAAACAGAGTAGAAA-CCCAAACAACCCGATTCAG-CAAAT----A
CBF3 -2496 AAAAAAAGTTTCCTAAAATAGTGTATAAAACCGAAACAAACTAATTCAA-CAAACC-CGA
CBF2 -2082 AAAAAATGTTGTTTAAATTAGAGTATAAAACCGAAACAACC-GATTCAGCAAAACCTCCA
******
T.arvense–2536 ATTTAATAGAGACC---CAAACGGTAATCCATAAACAGAA---AGTTGGGTCCCACAAGA
CBF1 -1217 ATTCAAAAACGAACGTCCGTACGATTTTCCAAAAACAGAA---AGATGGGTTCCACAAGA
CBF3 -2438 AATAAACAA--ATC---CGTACGACAA-CCAAAAATATCTTTCAGATGGGTTCCACAAGA
CBF2 -2021 AT--AATAG--ACC----GTAC----A-CCATAAACAGAA---ATATGGGTCCCACAAGA
T.arvense –2482 GCGAAAGTGGGCCCGTCAAAATATCCTAAACC-GTGGCCTAACAGCGGAAAC-GCTGTCC
CBF1 -1160 TAATGCGTGGGGACGTCAAAATCCTCTAAACCCGTGGTTCGGCCGCGGAAAC-ACTGTCC
CBF3 -2384 TA-ACCCAGTGCCAATCAGAAT--TCTGAAAGCGTGGCTCGACCGCGGAAACCATTGTCC
CBF2 -1979 GA------GC----AC---TGTCC
ABREATCONSENSUS
T.arvense -2424 CTACCCTTCCCCATCACTGGTCCCCT-ACGTGGCTCACTGTCGAGTTCCGTACCATATAT
CBF1 -1101 CTACC-TTCCCCACCAG------CCT------TCACTGGCC------CCATA---
CBF3 -2327 ATACC-TTCTCTTCTTTGTCCCCCCTTACGTGGCTCGCTGTGGAGTCTCGTACCACGT--
CBF2 -1968 GTAGC-TTCCCCTTCATTGGCCCTCT-ACGTGGCTC-CTCTTGT------AACCAAT---
*******
T.arvense -2364 AACACGTGTCACGTCACTTTCAAGCTTTACTTTCCTCTTAAGAAA-AACATATTATTATG
CBF1 -1068 ------CGTCACTCTCAAGCTTTACTTTC-TATTTTCCAC-TAAAGCCAATTTTG
CBF3 -2270 ------GTCGCGTCACT-TCACTCTTTACTTTC-TATTTTCCACTAAAATCATAATTTG
CBF2 -1920 ------GTCATGTCATTTTCAAGTTTTACTTTCTTTTTTTATACTAATATCTTGTT-TG
T.arvense–2306 TCATTTTCTGCACATTA----CC-A---CTCCTTTTTTCTTCCCATCTCTTTT-TGTCGT
CBF1 -1021 TTGTTTTCTTCACCTTA----CC-A---CTCTTTTTTTC--CC--TCTTTGTTGTGTCTT
CBF3 -2219 TCTTTTTCTTGACCATA----CCCA---CTC-TTTTTTCT-----TCTC-GTT--GTCGT
CBF2 -1866 TCGTTTTCTGTACCTTAAGGTCCTAAACCACTTTCTTTCGCGCACCATTCCTT--GTCGT
ABRELATERD1
T.arvense -2255 CTTGTTTCTCCAAAG-ATGTCAATAGAACGTGAGA-GCGC-TTGAGAGTGAGTGGTA---
CBF1 -997 CT--TTTCTCCTAA--ATGTCAATA--ACGTGAGA-GCG------AGAGGTA---
CBF3 -2175 CTTGCTTCTCCTAAATATCTCAA-ATAACGTGAGA-GAC--TTGAGTGTGAGAGGTAGGT
CBF2 -1810 ATTATTTCTCCGAA-TATGTCAATA--CCGTGAGACGACAATTGATAGCGAGAGGTAGCG
*****
T.arvense–2201 -ACGATACAGACGTTTTT-GTAAGCGAATATATATGCAAAGGAAGATATTCTGTAGAGAT
CBF1 -934 -ACGAGAGAGATATTTTT-GTCAGCGAATATAT-TTCA------TGCA-----
CBF3 -2119 AACGAGGCAGACTTTTTTTGGAAGCGAATATAACTT-A------TGCTGA---
CBF2 -1753 AGAGAGAGAAACGTTCGTTGT----GAAGATATTTT-A------T----TGCTG----
T.arvense-2143 ACTATTTTTGTTTTTGATGGAGAAAATAGAAAATCTTGTACATTGATTTGAACCTCCGAC
CBF1 -895 --TATCTT------ATTGTGAAGATT-----TTTTATACCTTTTTTTTGTC-----AA
CBF3 -2076 --TATTTT------ATTTAG---CTT-----TCTGAT---TGGAGTTGAGA------
CBF2 -1744 --T-TGTTG----AGATTTGAG---ATA-----TTTTATAGCTATTATTGGAA------
T.arvense -2083 TTCAGGACTCGCACCCACGTGATTAGATTTAAGCCACAAGTATTTTTCATGGATATTAT-
CBF1 -855 TACAATA-TAGCT------ATTA---TTGAGATTGAGATATTTTG--TGGGAATTAT-
CBF3 -2044 TATTATA-TAGGT------ATTA---TTGAGA------TATTATA--TACGTATTAT-
CBF2 -1676 TTTGAAAGT-GATGT------ATAAC--TT--GC------TACTATA--TCGGTCTAATC
T.arvense-2024 -TGAGATTTGA---GATATTTCATAGATATGTT-GTTGAGACTTGAGAGTGATGCATAAC
CBF1 -810 -TGGGATTCAA---GATAAC---TTGCTATTTT-GT-----ATTG-GTCTAATCCTTCGC
CBF3 -2005 -TGAGATTTGA---GATATTTTGTAGATTTTATAGTTCT--ATCG-GACTAATTCTTGGC
CBF2 -1635 CTTGGCTTAAACCAGTTTTTATTTAGTAATTTT-AT----GCTCCTGTATTATTATTCGG

T.arvense –1969 TTGCTATTGCGTCGGTCTAATATGATATA--TTATACTAATATACTATTTTAA-TCAATC
CBF1 -764 TTAGTCCTGTCCTGGTCCATTTACATGT---TTTTGGTTATAGTTTGTTTAAACTGAATA
CBF3 -1952 TTAAT------CCACTAACATGT---TTTTGTTTAG--T-TAATTAAACTGATT-
CBF2 -1580 TGGAGTATTTTTTTCTCAAAAAATATATGAGTCGAGTTTAAGAAATATATAAGCGAAAAA

T.arvense-1912 ATTTT-TACATGTATAAGAGGAAAACACTTGTGCATATGATATATTATACTA
CBF1 -707 ATTTTGTTCATC-ATAT--G-----CATT--T----ATGACTCATTTT----
CBF3 -1910 ATTTTCTG------
CBF2 -1520 ACAAAAAAAATATATATAAG-----CGGG-GT-----TAATAGATC------