Supplementary material:

Figure S1: Kinetics of soil hydrolytic enzymes (nmol/h/gC) in the litter (●) and soil organic horizon (▲) of a montainous spruce forest. Experimental data shown as the averages of three replicate samples were fitted to the nonlinear regression using equation (i) and (ii) using GraphPad Prism 4.0 software (full lines). Standard errors are shown as vertical lines.


Table S1: Potential activity of soil hydrolytic enzymes (umol/h/gC) in the litter and soil organic horizon of a mountainous spruce forest at measured at temperature range of 0°C to 25°C. Averages (n=12) and standard errors (%) are shown in parenthesis.

Assay temprature / 0°C / 5°C / 10°C / 15°C / 20°C / 25°C
Litter / betaglucosidase / 178
(19) / 225
(5) / 232
(25) / 263
(36) / 415
(17) / 674
(18)
phosphatase / 205
(36) / 224
(16) / 206
(38) / 241
(37) / 355
(20) / 582
(16)
cellobiosidase / 25
(16) / 22
(28) / 17
(78) / 15
(30) / 10
(43) / 24
(25)
alaninaminopeptidase / 188
(10) / 182
(2) / 152
(22) / 103
(13) / 80
(16) / 97
(26)
leucineaminopeptidase / 191
(13) / 170
(10) / 122
(25) / 95
(22) / 61
(26) / 62
(26)
Organic / betaglucosidase / 136
(50) / 179
(47) / 179
(20) / 186
(44) / 205
(43) / 500
(16)
phosphatase / 152
(76) / 231
(75) / 246
(38) / 295
(41) / 496
(34) / 701
(18)
cellobiosidase / 39
(44) / 41
(47) / 33
(16) / 24
(31) / 14
(46) / 24
(14)
alaninaminopeptidase / 241
(45) / 256
(42) / 175
(15) / 113
(52) / 84
(22) / 77
(71)
leucineaminopeptidase / 277
(44) / 289
(52) / 159
(11) / 96
(53) / 68
(24) / 43
(159)

Table S2: Q10 values for different temperature ranges in the litter and organic horizon.

Q10
Temperature range (°C) / 0-5 / 5-10 / 10-15 / 15-20 / 20-25
betaglucosidase / 1.60 / 1.06 / 1.29 / 2.49 / 2.64
phosphatase / 1.19 / 0.85 / 1.37 / 2.17 / 2.69
Litter / cellobiohydrolase / 0.77 / 0.60 / 0.78 / 0.44 / 5.76
alaninaminopeptidase / 0.94 / 0.70 / 0.46 / 0.60 / 1.47
leucineaminopeptidase / 0.79 / 0.52 / 0.61 / 0.41 / 1.03
betaglucosidase / 1.73 / 1.00 / 1.08 / 1.21 / 5.95
phosphatase / 2.31 / 1.13 / 1.44 / 2.83 / 2.00
Organic / cellobiohydrolase / 1.11 / 0.65 / 0.53 / 0.34 / 2.94
alaninaminopeptidase / 1.13 / 0.47 / 0.42 / 0.55 / 0.84
leucineaminopeptidase / 1.09 / 0.30 / 0.36 / 0.50 / 0.40

4