Exogenous Abscisic Acid Alleviates Cadmium Toxicity by Restricting Cd2+ Influx in Populus euphratica Cells
Yansha Han1, 2,†, Shaojie Wang1, †, Nan Zhao1, †, Shurong Deng1, Chenjing Zhao1, Nianfei Li1, Jian Sun3, Rui Zhao1, *, Huilan Yi2, Xin Shen1, Shaoliang Chen1
1 College of Biological Sciences and Technology (Box 162), Beijing Forestry University, Beijing 100083, P.R. China
2 School of Life Science, Shanxi University, Taiyuan 030006, Shanxi Province, P.R. China
3 School of Life Science, Jiangsu Normal University, Xuzhou 221116, Jiangsu Province, P.R. China
† These authors contributed equally to this work.
* Corresponding author.
Electronic Supplementary Data
Supplementary Fig. S1. Dose test of cadmium and ABA effect on cell growth of Populus euphratica.
Supplementary Fig. S2. Effect of ABA pretreatment on steady-state Cd2+ fluxes in P. euphratica cells under Cd2+ stress.
Supplementary Fig. S1. Dose test of cadmium and ABA effect on cell growth of Populus euphratica. P. euphratica cells were treated with CdCl2 (0, 25, 50, 100, and 200 μM) for 3 weeks in the presence or absence of 5 μM ABA. A. Representative images of cell appearance. B. Fresh weight of cells. Each column is the mean of three biologically independent samples, and bars represent the standard error of the mean. The asterisk (*) indicates significant difference between CdCl2 treatment with and without ABA (P<0.05) at each tested concentration.
Supplementary Fig. S2. Effect of ABA pretreatment on steady-state Cd2+ fluxes in P. euphratica cells under Cd2+ stress. Cells were pretreated with or without 5 μM ABA for 12 h, and then exposed to 0 or 100 μM CdCl2 for another 72 h. Steady-state Cd2+ fluxes were continuously recorded for 10 min for each cell. Each column represents the mean of 15 individual cells quantified from three biologically independent samples. Bars indicate the standard error of the mean. Different letters (a, b, c) denote significant differences at P<0.05.