ZAPNbr

Investigator

Study Report

No

Obsolete?

Bioassay, Mod1

BIOASSAY R1

Standard

Pathologist

DateEntered

06/23/2006

Necropsy Protocol

Trim Protocol

Remarks

NecropsyCharge

Remarks

End Date

Start Date

ACUC

Description

Treatment

Genotype

GroupName

Group ID

F01

+/+, +/+

F02

+/+, d/+

F03

d/+, +/+

F04

d/+, d/+

M01

+/+, +/+

M02

+/+, d/+

M03

d/+, +/+

M04

d/+, d/+

DateAdded

Investigator

Pathology

Necropsy

Email

Name

Necropsy Protocol

Bioassay, Mod1

General Instructions

False

Weigh Animal

Description

Freeze

Fixative

Organ

Weigh Organ

Measure Organ

Blood

Blood smear and CBC

False

False

10% NBF

Perform a complete necropsy including all 4 limbs.

False

False

Trim Protocol

BIOASSAY R1

1

Pathology/Histotechnology Lab, SAIC-Frederick, FNL

05/09/2012

ZAPNbr

Investigator

Study Report

No

Obsolete?

General Instructions

Lesions in protocol tissue will be put in appropriate protocol tissue numbered block labeled a, b, c, etc (ex: liver masses in blocks 7a, 7b, etc). Lesions in non-protocol tissue will be put in additional block(s) (ex: block 16, 17, etc)

Description

Organ

Block No.

00

N/A

Lesions designated by pathologist

01

Brain

4 cross sections to include cerebrum, thalamus, cerebellum, & medulla

02

Salivary Gland

Bilateral

02

LN, mandibular

Bilateral

02

Pancreas

03

Thyroid

(2); longitudinal

03

Trachea

1 cross section

03

Esophagus

1 cross section

03

Parathyroid

(2); longitudinal (with thyroid)

04

Adrenal

(2)

05

Pituitary

to include pars distalis, pars intermedia, and pars nervosa

06

Spleen

longitudinal

06

Thymus

06

LN, mesenteric

07

Gall Bladder

with median lobe of liver

07

Liver

(2): left lobe and median lobe

08

Heart

longitudinal

08

Kidney

1 cross, 1 longitudinal

08

Tongue

cross section

09

Lung

whole with bronchus

09

Skin/Subcutis

inguinal

09

Mammary Gland

inguinal, with skin

10

Stomach, glandular

longitudinal section to include pylorus and pyloric junction with duodenum.

10

Stomach, nonglandular

additional longitudinal section to include a portion of the glandular (fundic) stomach

10

Duodenum

cross section, with small portion of pancreas

10

Ileum

cross section

10

Rectum

cross section

11

Cecum

cross section

11

Colon

cross section of proximal

11

Jejunum

cross section

12

Testis

(2)

12

Urinary Bladder

cross section

12

Uterus

(2); cross section of each horn

12

Coagulating Gland

1 cross section; with seminal vesicle

12

Epididymis

(2); with testes

12

Ovary

(2)

12

Prostate

12

Seminal Vesicle

1 cross section

13

Harderian Gland

(2); in situ (with nasal sections/eye/head)

13

Nasal Sections

(4), 4th section to include eyes & harderian glands

13

Eye

(2); in situ (with nasal sections/head) with eyelids

14

Femur, Marrow

in situ (with femur)

14

Femur

include stifle joint

15

Vertebra

(3): cervical, thoracic & lumbar

15

Spinal Cord

(3); in situ (with vertebra)

2

Pathology/Histotechnology Lab, SAIC-Frederick, FNL

05/09/2012