Eurogentec - Custom Anti-Protein Polyclonal Antibody Production

Speedy discovery action

Anti-protein Order Form

Date: 11/14/2018

Eurogentec SA • Liege Science Park • Rue du Bois Saint-Jean 5 • B-4102 Seraing • Belgium • Tel +32 4 372 76 65 • Fax +32 4 264 07 88

RPM Liège • VAT (BE) 0427.348.346 • • •

Speedy discovery action

Anti-protein Order Form

Your Purchase Order Number (P.O.):

Price agreement / Quotation Number:

VAT Number:

(if VAT exempt please attach a copy of the certificate)

Eurogentec SA • Liege Science Park • Rue du Bois Saint-Jean 5 • B-4102 Seraing • Belgium • Tel +32 4 372 76 65 • Fax +32 4 264 07 88

RPM Liège • VAT (BE) 0427.348.346 • • •

Speedy discovery action

Anti-protein Order Form

Eurogentec SA • Liege Science Park • Rue du Bois Saint-Jean 5 • B-4102 Seraing • Belgium • Tel +32 4 372 76 65 • Fax +32 4 264 07 88

RPM Liège • VAT (BE) 0427.348.346 • • •

Speedy discovery action

Anti-protein Order Form

SHIPPING DETAILS

Last Name:

First Name:

University / Company:

Laboratory / Department:

Unit / Team:

Street and Number:

ZIP / Postal Code: City:

Country:

Tel:

Fax:

Email * :

Please note that incomplete forms could result in delays

* A valid e-mail address is mandatory as confirmations, schedule, ELISA and Purification reports will be sent electronically

INVOICING DETAILS

Last Name:

First Name:

University / Company:

Laboratory / Department:

Unit / Team:

Street and Number:

ZIP / Postal Code: City:

Country:

Tel:

Fax:

Email * :

Eurogentec SA • Liege Science Park • Rue du Bois Saint-Jean 5 • B-4102 Seraing • Belgium • Tel +32 4 372 76 65 • Fax +32 4 264 07 88

RPM Liège • VAT (BE) 0427.348.346 • • •

Speedy discovery action

Anti-protein Order Form

YOUR OWN ANTIGEN NAME:

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Type of antigen: Select one Purified Protein Peptide Coupled to Carrier Protein Free peptide to be coupled Inactivated Cells Inactivated Virus Polysaccharide Nucleic Acid Other

Total amount of antigen shipped: mgRequired storage temperature of antigen °C

In Solution. Number of tubes: Each tube contains mg/ml of antigen in ml buffer.

Buffer composition:

Please avoid detergents and aggressive chemicals like urea, acetic acid, guanidine hydrochloride,…

Lyophilised. Number of tubes: Each tube contains mg of antigen to solubilise in ml buffer.

Please note that PAGE slices are not compatible at all with the Speedy protocol.

Use µg / injection / host

If not mentioned, the recommended amount will be injected (see table below)

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HostType of host: Select one Rat (60 ug/injection)Guinea Pig (60 ug/injection)Rabbit (200 ug/injection)Number of hosts:

Additional Services (Charges Apply)

Screen pre-immune serum None 5 Animals (AS-PREI-05) 10 Animals (AS-PREI-10) 20 Animals (AS-PREI-20) Type of host:None Guinea Pig Rat Rabbit

ELISA testNone 1 animal, 1 antigen, 3 bleeds (AS-ELIS-01) 2 animals, 1 antigen, 2 bleeds (AS-ELIS-LL) 2 animals, 2 antigens, 2 bleeds (AS-ELIS-LLDX)

. (Especially recommended for programmes with purifications, otherwise serum will be pooled)

Total IgG PurificationNone 50 ml of serum (AS-PURI-PCIGG050)

Affinity Specific IgG Purification None 5 ml serum (5mg, AS-PURI-SM) 20 ml serum (8mg, AS-PURI-MED) 2nd 20 ml (AS-PURI-MED02) 50 ml serum (10mg, AS-PURI-01) 2nd 50 ml (AS-PURI-02) Number of purifications to run

If prior to the purification you do not indicate which serum to use, the purifications will be performed on the serum giving the best ELISA results if available, otherwise the serum from each host will be pooled prior to purification.

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Comments:

Animal Welfare Statement: I certify that the antigen is not dangerous for people or animals. The sample does not contain HIV or Hepatitis virus or non-purified proteins from such viruses. Recombinant proteins from human viruses will be accepted only if the production conditions are described and pathogen inactivation has been carried out. I certify that the antigen does not contain sodium azide.

Signed (mandatory) ______Date: ______

Getting started:

1. Please ship your antigen including a printed copy of this completed order form to

Eurogentec SA, Immunisation Department, Liege Science Park, Rue du Bois Saint-Jean 5, B-4102 Seraing, Belgium

1. For any further questions please contact us

Polyclonal Anti-Protein Antibody Production – Tips & Info

Required amount of antigen / max. volume

Size of antigen:> 18-20 kDa

per injection and per animal

Type of animal*amount of antigenrecommended amount of antigenmax. volume (solution or gel piece)

Rabbit 20- 200µg100µg 500µl

Guinea Pig 10- 50µg 30µg 250µl

Rat 10- 50µg 30µg 250µl

Mouse 2- 40µg 15µg 150µl

Hen 20- 200µg100µg 500µl

Goat/Sheep100-1000µg200µg1000µl

Size of antigen:< 18-20 kDa

per injection and per animal

Species*amount of antigenrecommended amount of antigenmax. volume (solution or gel piece)

Rabbit 40- 400µg200µg 500µl

Guinea Pig 20- 150µg 50µg 250µl

Rat 20- 150µg 50µg 250µl

Mouse 4- 60µg 40µg 150µl

Hen 40- 400µg200µg 500µl

Goat/Sheep200-1000µg400µg1000µl

EUROGENTEC Speedy immunisation protocol

Speedy (28 days)

Day0710182128

Injection1.2.3.4.

Bleedpre-immunemediumfinal

Antigen format / Shipment:

FormatShipmentRemarks

Lyophilisedat room temperature

Solutionon dry iceAvoid urea (max. 6M), acetic acid, guanidine hydro chloride, ..

If possible, please send pre-aliquoted samples

Contact / Shipping address:

Eurogentec SA • Liege Science Park • Rue du Bois Saint-Jean 5 • B-4102 Seraing • Belgium • Tel +32 4 372 76 65 • Fax +32 4 264 07 88

RPM Liège • VAT (BE) 0427.348.346 • • •

Speedy discovery action

Anti-protein Order Form

EUROGENTEC S.A.

Immunization Department

LIEGE Science Park

Rue du Bois Saint-Jean 5

4102 SERAING

Belgium

Orders: Fax: +32 4 264 0788

Web

Product information:

For a peptide design:

For ongoing programme information:

For latest offerings:

Eurogentec SA • Liege Science Park • Rue du Bois Saint-Jean 5 • B-4102 Seraing • Belgium • Tel +32 4 372 76 65 • Fax +32 4 264 07 88

RPM Liège • VAT (BE) 0427.348.346 • • •