Enzymessizegene Accession N Number Offungal Species Exhibitingcoverageidentity

Supplemental table:Scedosporium apiospermum antioxidant enzymes and fungal species exhibiting the closest orthologs.

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EnzymesSizeGene accession N°Number ofFungal species exhibitingCoverageIdentity

(AA)orthologsathe closest homologous proteinsrate

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Cu/Zn superoxide dismutases

KEZ45313.1258SAPIO_CDS2117264Colletotrichumincanum 100% 67%

Colletotrichumchlorophyti 100% 68%

Colletotrichumsimmondsii 100% 66%

Colletotrichumtofieldiae 100% 66%

Stachybotrys chlorohalonata 97% 70%

KEZ44265.1264SAPIO_CDS3212291Colletotrichum fioriniae 100% 54%

Colletotrichum nymphaeae 100% 54%

Colletotrichum simmondsii 100% 54%

Colletotrichum gloeosporioides 100% 52%

Colletotrichum orbiculare 100% 52%

KEZ43706.1363SAPIO_CDS4327103/c

KEZ41328.1154SAPIO_CDS7433308Thermothelomyces thermophila 99% 91%

Colletotrichum gloeosporioides 99% 89%

Thielavia terrestris 99% 86%

Colletotrichum sublineola 99% 85%

Tolypocladium ophioglossoides 100% 84%

Mn-superoxide dismutases

KEZ44426.1318SAPIO_CDS3426336Colletotrichum gloeosporioides 85% 65%

Fusarium graminearum 83% 63%

Tolypocladium ophioglossoides 83% 64%

Fusarium pseudograminearum 83% 63%

Colletotrichumchlorophyti 83% 65%

KEZ42057.1213SAPIO_CDS6474374Fusarium pseudograminearum 100% 79%

Fusarium verticillioides 100% 78%

Fusarium oxysporum f. sp. pisi 100% 78%

Trichoderma reesei 100% 78%

Fusarium proliferatum 100% 78%

KEZ40734.1231SAPIO_CDS8679273Colletotrichum gloeosporioides 100% 82%

Phaeoacremonium minimum 100% 80%

Verticillium alfalfae 100% 80%

Thermothelomyces thermophila 100% 80%

Podospora anserina 99% 77%

Catalases

KEZ44801.1725SAPIO_CDS2912154Neonectria ditissima 96% 66%

Colletotrichum higginsianum 96% 68%

Colletotrichum tofieldiae 97% 67%

Colletotrichum chlorophyti 97% 66%

Colletotrichum incanum 96% 67%

KEZ43973.1495SAPIO_CDS4185115Aspergillus cristatus 98% 71%

Aspergillus ruber 98% 71%

Aspergillus carbonarius 98% 72%

Sporothrix insectorum 99% 71%

Talaromyces stipitatus 99% 67%

KEZ38592.1749SAPIO_CDS10583199Drechmeria coniospora 98% 74%

Metarhizium acridum 98% 72%

Metarhizium album 98% 72%

Claviceps purpurea 98% 72%

Purpureocilliumlilacinum 100% 72%

/727SAPIO_CDS4198bUnknownMadurella mycetomatis 99% 82%

Chaetomiumcochliodes 99% 80%

Chaetomiumglobosum 99% 80%

Lepidopterellapalustris 99% 80%

Phaeomoniella chlamydospora 99% 79%

Glutathione peroxidases

KEZ43726.1229SAPIO_CDS4353264Glonium stellatum 99% 85%

Paraphaeosphaeria sporulosa 98% 85%

Leptosphaeria maculans 99% 84%

Stagonospora sp. 99% 83%

Cenococcum geophilum 99% 82%

KEZ39906.1169SAPIO_CDS8864217Tolypocladium ophioglossoides 99% 82%

Colletotrichum orchidophilum 99% 83%

Colletotrichum salicis 99% 83%

Colletotrichum fioriniae 99% 83%

Colletotrichum simmondsii 99% 82%

Glutaredoxins

KEZ46601.1106SAPIO_CDS0416165Pochonia chlamydosporia 99% 66%

Colletotrichum graminicola 99% 67%

Colletotrichum sublineola 99% 66%

Ustilaginoidea virens 99% 67%

Metarhizium guizhouense 99% 65%

KEZ44244.1258SAPIO_CDS3187330Phaeoacremonium minimum 100% 51%

Colletotrichum sublineola 99% 49%

Colletotrichum chlorophyti 99% 48%

Colletotrichum graminicola 99% 48%

Colletotrichum incanum 99% 48%

KEZ44471.1107SAPIO_CDS3482165Thielavia terrestris 98% 68%

Magnaporthe oryzae 99% 68%

Neurospora crassa 99% 68%

Thermothelomyces thermophila 99% 66%

Chaetomium thermophilum 97% 70%

KEZ40653.1156SAPIO_CDS8578244Emmonsia parva 98% 69%

Histoplasmacapsulatum 99% 67%

Blastomycespercursus 98% 68%

Madurella mycetomatis 99% 68%

Blastomyces dermatitidis 97% 70%

/266SAPIO_CDS3162bUnknownColletotrichum orchidophilum 96% 70%

Colletotrichumnymphaeae 96% 70%

Sordariamacrospora 96% 69%

Colletotrichumsalicis 96% 69%

Colletotrichumtofieldiae 96% 70%

Glutathione reductase

KEZ43605.1503SAPIO_CDS4534251Valsamali 100% 85%

Trichoderma gamsii 100% 83%

Pestalotiopsisfici 100% 87%

Trichoderma atroviride 100% 83%

Phaeoacremonium minimum 100% 85%

KEZ42753.1470SAPIO_CDS6039359Metarhizium anisopliae 100% 78%

Stachybotrys chlorohalonata 100% 78%

Fusarium oxysporum 100% 78%

Metarhizium brunneum 100% 78%

Metarhizium guizhouense 100% 78%

/477SAPIO_CDS4931bUnknownAspergillus wentii 99% 66%

Aspergillus lentulus 99% 65%

Aspergillus nomius 99% 68%

Aspergillus flavus 99% 67%

Aspergillus brasiliensis 99% 65%

Peroxiredoxins

KEZ43947.1218SAPIO_CDS4142208Pestalotiopsisfici 100% 86%

Colletotrichum gloeosporioides 100% 80%

Colletotrichumchlorophyti 100% 80%

Drechmeriaconiospora 100% 81%

Colletotrichum sublineola 100% 80%

KEZ39265.1166SAPIO_CDS9937366Verticilliumdahliae 98% 68%

Verticilliumlongisporum 98% 66%

Stachybotrys chlorohalonata 100% 63%

Colletotrichum higginsianum 100% 63%

Colletotrichumincanum 100% 63%

SaPrx2d159Not annotatedUnknownPochoniachlamydosporia 99% 69%

Acremonium chrysogenum 100% 65%

Aspergillus udagawae 99% 54%

Aspergillus fumigatus 100% 51%

Aspergillus lentulus 100% 52%

Thioredoxin

KEZ40673.1287SAPIO_CDS8608287Metarhizium acridum 95% 55%

Colletotrichum incanum 94% 55%

Purpureocillium lilacinum 96% 55%

Fusarium verticillioides 98% 54%

Metarhizium anisopliae 85% 59%

Thioredoxin reductases

KEZ45507.1322SAPIO_CDS183069Magnaporthe oryzae 89% 66%

Trichoderma virens 89% 65%

Aspergillus oryzae 88% 54%

Aspergillus flavus 88% 54%

Madurella mycetomatis 88% 52%

KEZ38934.1592SAPIO_CDS1027480Xylonaheveae 53% 55%

Aspergillus ochraceoroseus 53% 59%

Penicillium roqueforti 52% 55%

Metarhizium anisopliae 53% 55%

Metarhizium brunneum 53% 54%

Mitochondrial cytochrome C peroxidase

KEZ44632.1358SAPIO_CDS3675263Thielaviopsis punctulata 100% 77%

Colletotrichum graminicola 98% 75%

Ceratocystis platani 100% 74%

Colletotrichum sublineola 98% 75%

Fusarium proliferatum 99% 74%

Chloride peroxidase

KEZ39987.1246SAPIO_CDS8967127Colletotrichum chlorophyti 90% 64%

Aspergillus cristatus 98% 58%

Aspergillus bombycis 98% 59%

Colletotrichum orbiculare 92% 59%

Aspergillus glaucus 98% 58%

Flavohaemoglobin (nitric oxide dioxigenase)

/420SAPIO_CDS1359bUnknownPhaeoacremonium minimum 96% 65%

Fusarium langsethiae 99% 63%

Neonectria ditissima 99% 62%

Fusarium poae99% 63%

Nectria haematococca 99% 63%

/466SAPIO_CDS8682bUnknownColletotrichum fioriniae 91% 63%

Colletotrichumsimmondsii 91% 62%

Colletotrichumnymphaeae 91% 62%

Colletotrichumtofieldiae 91% 63%

Colletotrichumchlorophyti 91% 63%

Nitrate reductase

KEZ38947.1841SAPIO_CDS10293252Sporothrixschenckii 99% 68%

Sporothrixinsectorum 98% 67%

Doratomycessp. 98% 69%

Aspergillus luchuensis 98% 63%

Aspergillus kawachii 98% 63%

Nitrite reductase

KEZ38946.11084SAPIO_CDS10292254Doratomycessp. 95% 70%

Aspergillusfischeri 94% 68%

Aspergillus fumigatus 94% 68%

Aspergillus nomius 95% 67%

Aspergillus lentulus 98% 65%

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aDetermined using EnsemblFungi (available at

bIdentified as pseudogenes in the draft genome of S. apiospermum(likely because of the lack of introns), but a blastx analysis ( revealed high sequence homologies with numerous proteins annotated as the corresponding antioxidant enzymes in other fungal genomes.

cLess than 50% identity rate (with less than 65% coverage).

dGene not considered in the draft genome sequence as an open reading frame, and found only by a blast search on the whole genome sequence, located on contig JOWA01000107.1 (199186-199638).