English, Only the Common Crossbills Collected in England Were Genotyped;

Dawson DA, Horsburgh GJ, Küpper C, Stewart IRK, Ball AD, Durrant KL, Hansson B, Bacon I, Bird S, Klein Á, Lee J-W, Martín-Gálvez D, Simeoni M, Smith G, Spurgin LG, Burke T (2010) New methods to identify conserved microsatellite loci and develop primer sets of high utility – as demonstrated for birds. Molecular Ecology Resources. In press.

Supplementary Table S2 Characterization of 34 conserved EST microsatellite loci in four finch species when amplified using the conserved TG primer sets†

Locus / Fluoro label / PCR multiplex set members / Species / EoS / n / A / Exp. ZF allele size (bp) / Obs. allele size (bp) / Ho / He / pHW / Est. null allele freq.
TG01-000 / 6-FAM / 01-000 & 01-114 / Greenfinch / E / 21 / 8 / 253 / 238, 240, 242, 244, 246, 248, 250, 252 / 0.810 / 0.837 / 0.4160 / 0.0028
TG01-000 / 6-FAM / 01-000 & 01-114 / Crossbill / M / 17 / 5 / 253 / 240, 242, 244, 246, 248 / 0.706 / 0.772 / 0.0749 / 0.0268
TG01-000 / 6-FAM / 01-000 & 01-114 / Bullfinch / E / 22 / 6 / 253 / 238, 240, 242, 244, 246, 252 / 0.773 / 0.836 / 0.5883 / 0.0304
TG01-000 / 6-FAM / 01-000 & 01-114 / Chaffinch / E / 16 / 1 / 253 / 230 / 0 / 0 / - / 0
TG01-040 / 6-FAM / 01-040 & 02-078 / Greenfinch / H / 21 / 14 / 286 / 292, 293, 294, 295, 296, 298, 299, 300, 301, 305, 305, 308, 311, 317 / 0.810 / 0.873 / 0.5916 / 0.0329
TG01-040 / 6-FAM / 01-040 & 02-078 / Crossbill / E* / 17 / 3 / 286 / 290, 292, 294 / 0.529 / 0.668 / 0.0869* / 0.0948*
TG01-040 / 6-FAM / 01-040 & 02-078 / Bullfinch / M / 22 / 6 / 286 / 290, 292, 298, 300, 302, 304 / 0.636 / 0.691 / 0.1353 / 0.0399
TG01-040 / 6-FAM / 01-040 & 02-078 / Chaffinch / M / 17 / 8 / 286 / 284, 293, 296, 297, 298, 299, 300, 301 / 0.765 / 0.779 / 0.3085 / -0.0145
TG01-077 / 5HEX / 01-077 & 01-124 / Greenfinch / E / 20 / 2 / 153 / 149, 150 / 0.500 / 0.508 / 1 / -0.0050
TG01-077 / 5HEX / 01-077 & 01-124 / Crossbill / E / 17 / 1 / 153 / 150 / 0 / 0 / - / 0
TG01-077 / 5HEX / 01-077 & 01-124 / Bullfinch / E / 23 / 1 / 153 / 151 / 0 / 0 / - / 0
TG01-077 / 5HEX / 01-077 & 01-124 / Chaffinch / E / 18 / 1 / 153 / 148 / 0 / 0 / - / 0
TG01-092 / 6-FAM / 01-092 & 08-024 set 2 / Greenfinch / E / 17 / 1 / 183 / 181 / 0 / 0 / - / 0
TG01-092 / 6-FAM / 01-092 & 08-024 set 2 / Crossbill / E / 12 / 1 / 183 / 182 / 0 / 0 / - / 0
TG01-092 / 6-FAM / 01-092 & 08-024 set 2 / Bullfinch / E / 19 / 1 / 183 / 181 / 0 / 0 / - / 0
TG01-092 / 6-FAM / 01-092 & 08-024 set 2 / Chaffinch / E / 15 / 2 / 183 / 182, 184 / 0.067 / 0.067 / - / -0.0083
TG01-114 / 5HEX / 01-000 & 01-114 / Greenfinch / E / 21 / 3 / 182 / 179, 181, 183 / 0.286 / 0.324 / 0.5674 / 0.0438
TG01-114 / 5HEX / 01-000 & 01-114 / Crossbill / E / 17 / 2 / 182 / 179, 181 / 0.176 / 0.166 / 1 / -0.0388
TG01-114 / 5HEX / 01-000 & 01-114 / Bullfinch / E / 23 / 2 / 182 / 183, 185 / 0.217 / 0.264 / 0.4141 / 0.0855
TG01-114 / 5HEX / 01-000 & 01-114 / Chaffinch / E / 19 / 1 / 182 / 181 / 0 / 0 / - / 0
TG01-124 / 6-FAM / 01-077 & 01-124 / Greenfinch / E / 21 / 3 / 403 / 404, 406, 408 / 0.190 / 0.298 / 0.0128 / 0.2507
TG01-124 / 6-FAM / 01-077 & 01-124 / Crossbill / E / 17 / 3 / 403 / 405, 406, 407 / 0.176 / 0.269 / 0.1250 / 0.1841
TG01-124 / 6-FAM / 01-077 & 01-124 / Bullfinch / E / 23 / 3 / 403 / 400, 402, 406 / 0.348 / 0.389 / 0.5019 / 0.0657
TG01-124 / 6-FAM / 01-077 & 01-124 / Chaffinch / 15 / 3 / 403 / 401, 403, 405 / 0.200 / 0.297 / 0.3008 / 0.1717
TG01-147 / 5HEX / 01-147 & 02-120 / Greenfinch / E / 21 / 1 / 283 / 278 / 0 / 0 / - / 0
TG01-147 / 5HEX / 01-147 & 02-120 / Crossbill / E / 16 / 2 / 283 / 278, 280 / 0.500 / 0.484 / 1 / -0.0323
TG01-147 / 5HEX / 01-147 & 02-120 / Bullfinch / E / 22 / 1 / 283 / 288 / 0 / 0 / - / 0
TG01-147 / 5HEX / 01-147 & 02-120 / Chaffinch / E / 20 / 4 / 283 / 276, 278, 280, 282 / 0.650 / 0.696 / 0.2649 / 0.0144
TG01-148 / 5HEX / 01-148 & 11-011 / Greenfinch / E / 21 / 5 / 195 / 193, 195, 197, 199, 201 / 0.857 / 0.698 / 0.2853 / -0.1322
TG01-148 / 5HEX / 01-148 & 11-011 / Crossbill / M / 16 / 7 / 195 / 195, 197, 199, 200, 201, 203, 205 / 0.813 / 0.829 / 0.0147 / -0.0277
TG01-148 / 5HEX / 01-148 & 11-011 / Bullfinch / E / 21 / 4 / 195 / 195, 197, 199, 201 / 0.524 / 0.683 / 0.4583 / 0.1192
TG01-148 / 5HEX / 01-148 & 11-011 / Chaffinch / E / 19 / 4 / 195 / 185, 187, 189, 191 / 0.316 / 0.440 / 0.2022 / 0.1306
TG02-078 / 5HEX / 01-040 & 02-078 / Greenfinch / O / 19 / 2 / 308 / 288, 289 / 0.316 / 0.478 / 0.1659 / 0.1915
TG02-078 / 5HEX / 01-040 & 02-078 / Crossbill / E / 17 / 3 / 308 / 288, 289, 290 / 0.176 / 0.169 / 1 / -0.0377
TG02-078 / 5HEX / 01-040 & 02-078 / Bullfinch / M, O / 23 / 2 / 308 / 288, 289 / 0.130 / 0.198 / 0.2167 / 0.1951
TG02-078 / 5HEX / 01-040 & 02-078 / Chaffinch / M, O / 17 / 4 / 308 / 286, 288, 289, 293 / 0.176 / 0.171 / 1 / -0.0372
TG02-088 / 6-FAM / 02-088 & 04-004 / Greenfinch / M / 20 / 11 / 266 / 272, 274, 284, 286, 288, 291, 293, 295, 297, 299, 312 / 0.900 / 0.879 / 0.2233 / -0.0317
TG02-088 / 6-FAM / 02-088 & 04-004 / Crossbill / M / 16 / 8 / 266 / 245, 257, 268, 270, 272, 274, 276, 278 / 0.750 / 0.792 / 0.8926 / 0.0103
TG02-088 / 6-FAM / 02-088 & 04-004 / Bullfinch / M / 22 / 10 / 266 / 274, 276, 278, 280, 282, 284, 286, 288, 291, 293 / 0.818 / 0.857 / 0.0752 / 0.0096
TG02-088 / 6-FAM / 02-088 & 04-004 / Chaffinch / M / 19 / 6 / 266 / 261, 263, 265, 267, 269, 271 / 0.789 / 0.780 / 0.0675 / -0.0381
TG02-120 / 6-FAM / 01-147 & 02-120 / Greenfinch / E / 21 / 2 / 230 / 229, 231 / 0.143 / 0.136 / 1 / -0.0286
TG02-120 / 6-FAM / 01-147 & 02-120 / Crossbill / E / 16 / 4 / 230 / 222. 225. 231. 233 / 0.313 / 0.375 / 0.5624 / 0.0599
TG02-120 / 6-FAM / 01-147 & 02-120 / Bullfinch / E / 22 / 4 / 230 / 233, 234, 237, 239 / 0.227 / 0.214 / 1 / -0.0524
TG02-120 / 6-FAM / 01-147 & 02-120 / Chaffinch / E / 20 / 2 / 230 / 231, 233 / 0.050 / 0.050 / - / -0.0050
TG03-002 / 6-FAM / 03-002 & 04-012 / Greenfinch / M / 19 / 7 / 127 / 123, 124, 125, 126, 127, 128, 130 / 0.895 / 0.728 / 0.4623 / -0.1618
TG03-002 / 6-FAM / 03-002 & 04-012 / Crossbill / M / 17 / 4 / 127 / 124, 126, 128, 130 / 0.529 / 0.619 / 0.3411 / 0.0672
TG03-002 / 6-FAM / 03-002 & 04-012 / Bullfinch / E / 23 / 3 / 127 / 126, 128, 130 / 0.348 / 0.368 / 0.0932 / -0.0019
TG03-002 / 6-FAM / 03-002 & 04-012 / Chaffinch / E / 18 / 3 / 127 / 120, 122, 126 / 0.333 / 0.294 / 1 / -0.0858
TG03-031 / 6-FAM / 03-031 & 07-022 / Greenfinch / E / 20 / 2 / 208 / 198, 200 / 0.250 / 0.358 / 0.2146 / 0.1649
TG03-031 / 6-FAM / 03-031 & 07-022 / Crossbill / E / 17 / 2 / 208 / 198, 200 / 0.056 / 0.056 / - / -0.0061
TG03-031 / 6-FAM / 03-031 & 07-022 / Bullfinch / E / 22 / 1 / 208 / 198 / 0 / 0 / - / 0
TG03-031 / 6-FAM / 03-031 & 07-022 / Chaffinch / E / 17 / 1 / 208 / 198 / 0 / 0 / - / 0
TG03-034 / 6-FAM / 03-034 & 12-015 / Greenfinch / E / 21 / 2 / 178 / 175, 177 / 0.190 / 0.251 / 0.3398 / 0.1250
TG03-034 / 6-FAM / 03-034 & 12-015 / Crossbill / E / 16 / 1 / 178 / 177 / 0 / 0 / - / 0
TG03-034 / 6-FAM / 03-034 & 12-015 / Bullfinch / E / 23 / 2 / 178 / 177, 179 / 0.043 / 0.043 / - / -0.0039
TG03-034 / 6-FAM / 03-034 & 12-015 / Chaffinch / E / 16 / 1 / 178 / 177 / 0 / 0 / - / 0
TG03-035 / 5HEX / 03-035 & 04-041 / Greenfinch / H / 16 / 6 / 213 / 200, 221, 222, 223, 224, 226 / 0.750 / 0.768 / 0.5605 / -0.0132
TG03-035 / 5HEX / 03-035 & 04-041 / Crossbill / H / 14 / 2 / 213 / 217, 220 / 0.143 / 0.138 / 1 / -0.0286
TG03-035 / 5HEX / 03-035 & 04-041 / Bullfinch / E / 20 / 1 / 213 / 214 / 0 / 0 / - / 0
TG03-035 / 5HEX / 03-035 & 04-041 / Chaffinch / E / 20 / 1 / 213 / 216 / 0 / 0 / - / 0
TG03-098 / 5HEX / 03-098 & Z-037 / Greenfinch / E / 21 / 3 / 235 / 240, 242, 244 / 0.429 / 0.556 / 0.0379 / 0.1005
TG03-098 / 5HEX / 03-098 & Z-037 / Crossbill / E / 15 / 5 / 235 / 234, 235, 237, 238, 242 / 0.467 / 0.568 / 0.1974 / 0.0483
TG03-098 / 5HEX / 03-098 & Z-037 / Bullfinch / M / 22 / 4 / 235 / 238, 239, 240, 241 / 0.545 / 0.690 / 0.0997 / 0.1108
TG03-098 / 5HEX / 03-098 & Z-037 / Chaffinch / M / 19 / 6 / 235 / 234, 235, 236, 237, 238, 239 / 0.789 / 0.777 / 0.4007 / -0.0089
TG04-004 / 5HEX / 02-088 & 04-004 / Greenfinch / E / 21 / 2 / 166 / 166, 168 / 0.048 / 0.048 / - / -0.0046
TG04-004 / 5HEX / 02-088 & 04-004 / Crossbill / E / 16 / 7 / 166 / 156, 164, 170, 172, 174, 176, 178 / 0.875 / 0.768 / 0.9874 / -0.0839
TG04-004 / 5HEX / 02-088 & 04-004 / Bullfinch / M, O / 23 / 3 / 166 / 160, 166, 168 / 0.130 / 0.127 / 1 / -0.0244
TG04-004 / 5HEX / 02-088 & 04-004 / Chaffinch / E / 20 / 3 / 166 / 166, 168, 170 / 0.150 / 0.232 / 0.1102 / 0.1944
TG04-012 / 5HEX / 03-002 & 04-012 / Greenfinch / E / 20 / 3 / 150 / 146, 148, 150 / 0.500 / 0.445 / 0.4438 / -0.0793
TG04-012 / 5HEX / 03-002 & 04-012 / Crossbill / E / 17 / 4 / 150 / 143, 146, 147, 148 / 0.471 / 0.651 / 0.3253 / 0.1488
TG04-012 / 5HEX / 03-002 & 04-012 / Bullfinch / E / 23 / 5 / 150 / 138, 140, 142, 143, 144 / 0.739 / 0.645 / 0.9458 / -0.0839
TG04-012 / 5HEX / 03-002 & 04-012 / Chaffinch / E / 19 / 5 / 150 / 137, 139, 140, 141, 143 / 0.684 / 0.707 / 0.7076 / 0.0160
TG04-012a / 6-FAM / 04-012a & 13-009 / Greenfinch / E / 20 / 1 / 236 / 235 / 0 / 0 / - / 0
TG04-012a / 6-FAM / 04-012a & 13-009 / Crossbill / E / 16 / 3 / 236 / 231, 235, 239 / 0.250 / 0.232 / 1 / -0.0600
TG04-012a / 6-FAM / 04-012a & 13-009 / Bullfinch / E / 23 / 1 / 236 / 235 / 0 / 0 / - / 0
TG04-012a / 6-FAM / 04-012a & 13-009 / Chaffinch / E / 20 / 1 / 236 / 235 / 0 / 0 / - / 0
TG04-041 / 5HEX / 03-035 & 04-041 / Greenfinch / E / 19 / 2 / 173 / 172, 176 / 0.158 / 0.149 / 1 / -0.0331
TG04-041 / 5HEX / 03-035 & 04-041 / Crossbill / E / 17 / 1 / 173 / 176 / 0 / 0 / - / 0
TG04-041 / 5HEX / 03-035 & 04-041 / Bullfinch / E / 20 / 1 / 173 / 176 / 0 / 0 / - / 0
TG04-041 / 5HEX / 03-035 & 04-041 / Chaffinch / E / 20 / 2 / 173 / 174, 176 / 0.050 / 0.050 / - / -0.0050
TG04-061 / 5HEX / 04-061 & 09-014 & 13-016 / Greenfinch / E / 20 / 4 / 186 / 191, 192, 193, 194 / 0.350 / 0.491 / 0.2018 / 0.1458
TG04-061 / 5HEX / 04-061 & 09-014 & 13-016 / Crossbill / E / 16 / 4 / 186 / 191, 192, 194, 195 / 0.688 / 0.569 / 0.5234 / -0.1226
TG04-061 / 5HEX / 04-061 & 09-014 & 13-016 / Bullfinch / H / 22 / 5 / 186 / 189, 190, 191, 192, 193 / 0.909 / 0.743 / 0.3395 / -0.1154
TG04-061 / 5HEX / 04-061 & 09-014 & 13-016 / Chaffinch / E / 20 / 4 / 186 / 193, 194, 195, 196 / 0.550 / 0.560 / 0.4186 / -0.0362
TG05-030 / 5HEX / 05-030 & 08-024 / Greenfinch / E / 20 / 3 / 186 / 181, 183, 185 / 0.400 / 0.344 / 1 / -0.1044
TG05-030 / 5HEX / 05-030 & 08-024 / Crossbill / E / 16 / 2 / 186 / 181, 183 / 0.125 / 0.121 / 1 / -0.0234
TG05-030 / 5HEX / 05-030 & 08-024 / Bullfinch / E / 23 / 2 / 186 / 183, 185 / 0.087 / 0.085 / 1 / -0.0131
TG05-030 / 5HEX / 05-030 & 08-024 / Chaffinch / E / 20 / 2 / 186 / 181, 183 / 0.500 / 0.492 / 1 / -0.0204
TG05-046 / 6-FAM / 05-046 & 22-001 / Greenfinch / E / 20 / 1 / 337 / 331 / 0 / 0 / - / 0
TG05-046 / 6-FAM / 05-046 & 22-001 / Crossbill / E / 17 / 1 / 337 / 331 / 0 / 0 / - / 0
TG05-046 / 6-FAM / 05-046 & 22-001 / Bullfinch / E / 22 / 1 / 337 / 332 / 0 / 0 / - / 0
TG05-046 / 6-FAM / 05-046 & 22-001 / Chaffinch / E / 20 / 2 / 337 / 333, 335 / 0.450 / 0.409 / 1 / -0.0604
TG05-053 / 6-FAM / 05-053 & 06-009 & 13-017 / Greenfinch / M / 20 / 7 / 196 / 203, 204, 205, 206 207, 208, 209 / 1.000 / 0.785 / 0.0667 / -0.1418
TG05-053 / 6-FAM / 05-053 & 06-009 & 13-017 / Crossbill / E / 16 / 3 / 196 / 199, 201, 209 / 0.188 / 0.179 / 1 / -0.0410
TG05-053 / 6-FAM / 05-053 & 06-009 & 13-017 / Bullfinch / M, O / 21 / 3 / 196 / 192, 193, 194 / 0.524 / 0.528 / 0.6815 / -0.0158
TG05-053 / 6-FAM / 05-053 & 06-009 & 13-017 / Chaffinch / E / 19 / 4 / 196 / 198, 199, 200, 201 / 0.632 / 0.681 / 0.3048 / 0.0028
TG06-009 / 6-FAM / 05-053 & 06-009 & 13-017 / Greenfinch / E / 20 / 2 / 127 / 121, 123 / 0.050 / 0.050 / - / -0.0050
TG06-009 / 6-FAM / 05-053 & 06-009 & 13-017 / Crossbill / E / 16 / 1 / 127 / 121 / 0 / 0 / - / 0
TG06-009 / 6-FAM / 05-053 & 06-009 & 13-017 / Bullfinch / M, O / 22 / 3 / 127 / 123, 125, 127 / 0.409 / 0.413 / 0.3046 / 0.0338
TG06-009 / 6-FAM / 05-053 & 06-009 & 13-017 / Chaffinch / M, O / 20 / 3 / 127 / 123, 125, 127 / 0.450 / 0.376 / 1 / -0.1196
TG07-022 / 5HEX / 03-031 & 07-022 / Greenfinch / E / 20 / 2 / 416 / 414. 416 / 0.200 / 0.185 / 1 / -0.0462
TG07-022 / 5HEX / 03-031 & 07-022 / Crossbill / E / 17 / 3 / 416 / 411, 413, 415 / 0.588 / 0.551 / 1 / -0.0505
TG07-022 / 5HEX / 03-031 & 07-022 / Bullfinch / E / 22 / 4 / 416 / 407, 415, 417, 419 / 0.545 / 0.443 / 0.7052 / -0.1441
TG07-022 / 5HEX / 03-031 & 07-022 / Chaffinch / E / 16 / 3 / 416 / 407, 408, 410 / 0.125 / 0.123 / 1 / -0.0228
TG08-024 set 1 / 5HEX / 05-030 & 08-024 / Greenfinch / E / 21 / 4 / 128 / 122, 123, 124, 125 / 0.619 / 0.619 / 0.2915 / -0.0219
TG08-024 set 1 / 5HEX / 05-030 & 08-024 / Crossbill / M / 15 / 3 / 128 / 122, 123, 124 / 0.400 / 0.432 / 0.7155 / 0.0057
TG08-024 set 1 / 5HEX / 05-030 & 08-024 / Bullfinch / E / 23 / 2 / 128 / 123, 124 / 0.087 / 0.085 / 1 / -0.0131
TG08-024 set 1 / 5HEX / 05-030 & 08-024 / Chaffinch / E / 19 / 3 / 128 / 123, 124, 125 / 0.211 / 0.198 / 1 / -0.0481
TG08-024 set 2 / 5HEX / 01-092 & 08-024 set 2 / Greenfinch / E / 15 / 3 / 243 / 237, 238, 239 / 0.667 / 0.591 / 0.7142 / -0.0727
TG08-024 set 2 / 5HEX / 01-092 & 08-024 set 2 / Crossbill / H / (9) / (3) / 243 / (235, 239, 240). Poor amplification and difficult to score in multiplex. / (0.556) / (0.471) / (0.7206) / Not done
TG08-024 set 2 / 5HEX / Singleplex / Crossbill (Eng;ish) / E / 10 / 2 / 243 / 239, 240 / 0.500 / 0.521 / 1 / -0.0050
TG08-024 set 2 / 5HEX / 01-092 & 08-024 set 2 / Bullfinch / E / 19 / 3 / 243 / 239, 240, 241 / 0.368 / 0.391 / 0.7146 / 0.0025
TG08-024 set 2 / 5HEX / 01-092 & 08-024 set 2 / Chaffinch / E / 13 / 5 / 243 / 238, 239, 240, 241, 242 / 0.846 / 0.640 / 0.7474 / -0.2152
TG09-014 / 6-FAM / 04-061 & 09-014 & 13-016 / Greenfinch / E / 20 / 1 / 152 / 150 / 0 / 0 / - / -
TG09-014 / 6-FAM / 04-061 & 09-014 & 13-016 / Crossbill / E / 17 / 1 / 152 / 150 / 0 / 0 / - / -
TG09-014 / 6-FAM / 04-061 & 09-014 & 13-016 / Bullfinch / E / 22 / 1 / 152 / 150 / 0 / 0 / - / -
TG09-014 / 6-FAM / 04-061 & 09-014 & 13-016 / Chaffinch / E / 20 / 1 / 152 / 150 / 0 / 0 / - / -
TG11-011 / 6-FAM / 01-148 & 11-011 / Greenfinch / H (weak) / 15 / 17 / 223 / 232, 233, 245, 249, 251, 253, 255, 257, 261, 263, 265, 266, 269, 271, 273, 275, 281 / 0.933 / 0.956 / 0.0921 / -0.0052
TG11-011 / 6-FAM / 01-148 & 11-011 / Crossbill / M / 15 / 16 / 223 / 228, 230, 233, 234, 235, 236, 237, 238, 240, 241, 242, 243, 244, 248, 249, 255 / 0.800 / 0.943 / 0.0590 / 0.0662
TG11-011 / 6-FAM / Singleplex / Crossbill (English) / M / 10 / 7 / 223 / 230, 234, 236, 240, 242, 244, 258 / 1.000 / 0.858 / 0.3502 / -0.1062
TG11-011 / 6-FAM / 01-148 & 11-011 / Bullfinch / M / 17 / 7 / 223 / 230, 231, 232, 233, 234, 235, 237 / 0.353 / 0.499 / 0.0725 / 0.1930
TG11-011 / 6-FAM / 01-148 & 11-011 / Chaffinch / M / 10 / 5 / 223 / 217, 219, 221, 225, 227 / 0.900 / 0.721 / 0.5932 / -0.1676
TG11-011 / 6-FAM / Singleplex / Chaffinch / E / 20 / 7 / 223 / 215, 217, 219, 221, 223, 225, 227 / 0.900 / 0.777 / 0.8604 / -0.0907
TG12-015 / 5HEX / 03-034 & 12-015 / Greenfinch / E / 21 / 3 / 283 / 281, 283, 285 / 0.476 / 0.424 / 0.7845 / -0.0602
TG12-015 / 5HEX / 03-034 & 12-015 / Crossbill / E / 16 / 3 / 283 / 280, 282, 284 / 0.188 / 0.179 / 1 / -0.0410
TG12-015 / 5HEX / 03-034 & 12-015 / Bullfinch / M, (O) / 23 / 2 / 283 / 281, 283 / 0.348 / 0.464 / 0.3547 / 0.1321
TG12-015 / 5HEX / 03-034 & 12-015 / Chaffinch / M, (O) / 16 / 4 / 283 / 278, 279, 280, 284 / 0.625 / 0.599 / 0.9027 / -0.0355
TG13-009 / 5HEX / 04-012a & 13-009 / Greenfinch / E / 20 / 3 / 195 / 189, 195, 197 / 0.100 / 0.145 / 0.0820 / 0.2897
TG13-009 / 5HEX / 04-012a & 13-009 / Crossbill / E / 16 / 2 / 195 / 195, 197 / 0.125 / 0.121 / 1 / -0.0234
TG13-009 / 5HEX / 04-012a & 13-009 / Bullfinch / E / 23 / 2 / 195 / 195, 197 / 0.087 / 0.085 / 1 / -0.0131
TG13-009 / 5HEX / 04-012a & 13-009 / Chaffinch / E / 18 / 2 / 195 / 196, 198 / 0.278 / 0.246 / 1 / -0.0708
TG13-016 / 6-FAM / Singleplex / Greenfinch / E / 19 / 2 / 136 / 129, 130 / 0.053 / 0.053 / - / -0.0055
TG13-016 / 6-FAM / Singleplex / Crossbill / E / 16 / 3 / 136 / 129, 130, 131 / 0.188 / 0.179 / 1 / -0.0410
TG13-016 / 6-FAM / Singleplex / Bullfinch / E / 23 / 1 / 136 / 129 / 0 / 0 / - / -
TG13-016 / 6-FAM / Singleplex / Chaffinch / E / 20 / 2 / 136 / 128, 129 / 0.053 / 0.053 / - / -0.0055
TG13-017 / 6-FAM / 05-053 & 06-009 & 13-017 / Greenfinch / M / 20 / 10 / 300 / 297, 298, 299, 300, 301, 302, 303, 304, 305, 307 / 0.900 / 0.886 / 0.8219 / -0.0268
TG13-017 / 6-FAM / 05-053 & 06-009 & 13-017 / Crossbill / H / 18 / - / 300 / Unscorable in multiplex due to multiple peaks. / - / - / - / -
TG13-017 / 6-FAM / Singleplex / Crossbill (English) / H / 10 / - / 300 / Unscorable in singleplex due to multiple peaks. / - / - / - / -
TG13-017 / 6-FAM / 05-053 & 06-009 & 13-017 / Bullfinch / H / 20 / 6 / 300 / 317, 319, 320, 321, 323, 325 / 0.750 / 0.672 / 0.9752 / -0.0652
TG13-017 / 6-FAM / 05-053 & 06-009 & 13-017 / Chaffinch / M / 19 / 3 / 300 / 281, 282, 283 / 0.263 / 0.240 / 1 / -0.0643
TG22-001 / 5HEX / 05-046 & 22-001 / Greenfinch / E / 20 / 7 / 269 / 260, 270, 272, 274, 276, 278, 280 / 0.700 / 0.758 / 0.5161 / 0.0286
TG22-001 / 5HEX / 05-046 & 22-001 / Crossbill / M / 16 / 13 / 269 / 280, 282, 286, 288, 290, 292, 298, 300, 309, 311, 317, 321, 323 / 0.750 / 0.889 / 0.2332 / 0.0726
TG22-001 / 5HEX / 05-046 & 22-001 / Bullfinch / M / 21 / 17 / 269 / 259, 265, 267, 269, 273, 275, 277, 281, 287, 289, 295, 297, 299, 303, 307, 311, 328 / 0.952 / 0.929 / 0.8861 / -0.0240
TG22-001 / 5HEX / 05-046 & 22-001 / Chaffinch / M / 19 / 8 / 269 / 248, 252, 270, 272, 274, 276, 278, 300 / 0.632 / 0.760 / 0.2950 / 0.0930

†Each locus was tested in 21 greenfinch Carduelis chloris, 17 common crossbill Loxia curvirostra, 23 Eurasian bullfinch Pyrrhula pyrrhula and 20 chaffinch Fringilla coelebs individuals;

English, only the common crossbills collected in England were genotyped;

The PCR program used for all loci was 95ºC for 15 min followed by 35 cycles of 94ºC for 30 s, 56ºC for 90 s, 72ºC for 60 s, a final extension step of 60ºC for 6 min and an ambient holding temperature;

EoS, ease of scoring: E=easy, M=medium, H=hard, O=overloaded, (O)=some overloading;

*, difficult to score consistently (locus TG01-040 in common crossbill);

n, number of unrelated finch individuals genotyped; A, number of alleles; HO, observed heterozygosity; HE, expected heterozygosity;

pHW, probability of deviation from Hardy-Weinberg expectations (p-value, calculated using GENEPOP v3.4, Raymond & Rousset 1995). After a sequential Bonferroni correction for multiple testing none of the loci deviated significantly from Hardy-Weinberg expectations.

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