Electronic Supporting Information

Electronic SupplementaryInformation

Improvement of the Thermal Stability of Streptavidin Immobilized on Magnetic Beads by the Construction of a Mixed-poly (ethylene glycol) Tethered-chain Layer

Masaki Kubota, Keitaro Yoshimoto, Xiaofei Yuan, Yukio Nagasaki*

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Masaki Kubota, Keitaro Yoshimoto, Xiaofei Yuan, Yukio Nagasaki

Graduate School of Pure and Applied Science, University of Tsukuba, Tennoudai 1-1-1, Tsukuba, Ibaraki 305-8573, Japan

Fax: +81-29-853-5749 ; e-mail:

Keitaro Yoshimoto, Xiaofei Yuan, Yukio Nagasaki

Center for Tsukuba Advanced Research Alliance (TARA), University of Tsukuba, Tennoudai 1-1-1, Tsukuba 305-8577, Japan

Tsukuba Research Center for Interdisciplinary Materials Science (TIMS), University of Tsukuba, Tennoudai 1-1-1, Tsukuba, Ibaraki, 305-8573, Japan

Yukio Nagasaki

Master’s School of Medical Science, Graduate School of Comprehensive Human Sciences, University of Tsukuba, Tennoudai 1-1-1, Tsukuba, Ibaraki 305-8573, Japan

Satellite Laboratory, International Center for Materials Nanoarchilectonics (MANA), National Institute of Materials Science (NIMS), Tennoudai 1-1-1, Tsukuba, Ibaraki 305-8573, Japan

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Supplementary information

Figure S1. Changes in the amount of biotinylated ssDNA captured by SA-MB as a function of the streptavidin concentration in the preparation. The amounts of immobilized streptavidin were estimated based on the amount of ssDNA by SA-MB captured. The number of binding sites of streptavidin on SA-MB was assumed three.

Figure S2. The amount of biotinylated ssDNA captured by PEG/SA-MB and SA-MB. Streptavidin concentration in preparation step is 0.2 μM.