Electronic Supplementary Materials s7

Electronic Supplementary Materials

Comparative Proteome Analysis of Robust Saccharomyces cerevisiae insights into Industrial Continuous and Batch Fermentation

Jing-Sheng Cheng, Bin Qiao, Ying-Jin Yuan*

Table S1. Proteins identified by 2-DE combined with MALDI-TOF-MS. Corresponding spots are indicated in Fig. 4

Spot / gi No. / Gene name / Description / Cov.
(%) / Matched Peptide Numbers / Spiecies
Proteins involved in glycolysis and gluconeogenesis pathway
114 / 6323073 / PDC1 / Pyruvate decarboxylase isozyme 1 / 63 / 34 / S. cerevisiae
115 / 6323073 / PDC1 / Pyruvate decarboxylase isozyme 1 / 50 / 23 / S. cerevisiae
116 / 6323073 / PDC1 / Pyruvate decarboxylase isozyme 1 / 60 / 7 / S. cerevisiae
6 / 929845 / ADH1 / Alcohol dehydrogenase / 69 / 6 / S.cerevisiae
17 / 45269553 / TDH3 / Glyceraldehyde-3-phosphate dehydrogenase 3 / 34 / 13 / S.cerevisiae
26 / 6320255 / TPI1 / Triosephosphate isomerase / 30 / 6 / S.cerevisiae
71 / 230405 / TPI1 / Triosephosphate isomerase / 75 / 12 / S. cerevisiae
85 / 6321968 / ENO2 / Eno2p 2 / 43 / 16 / S.cerevisiae
86 / 6321968 / ENO2 / Eno2p 2 / 35 / 16 / S.cerevisiae
88 / 6322790 / FBA1 / Fructose-bisphosphate aldolase / 56 / 15 / S.cerevisiae
73 / 10383781 / PGK1 / Phosphoglycerate kinase / 57 / 21 / S.cerevisiae
78 / 10383781 / PGK1 / Phosphoglycerate kinase / 68 / 26 / S.cerevisiae
101 / 157833610 / PGK1 / Phosphoglycerate kinase / 42 / 12 / S. cerevisiae
81 / 6319809 / GLK1 / Glucokinase / 49 / 18 / S.cerevisiae
82 / 6319673 / PGI1 / Glucose-6-phosphate isomerase / 57 / 24 / S.cerevisiae
83 / 6319673 / PGI1 / Glucose-6-phosphate isomerase / 61 / 26 / S.cerevisiae
95 / 20151217 / ENO1 / Enolase 1 / 41 / 13 / S. cerevisiae
Proteins related to pentose phosphate pathway and glycerol biosynthetic process
90 / 6323386 / TAL1 / Transaldolase / 63 / 24 / S.cerevisiae
89 / 86558907 / RHR2 (GPP1) / (DL)-glycerol-3-phosphatase 1 / 77 / 17 / S. cerevisiae
Proteins related to stress responses
32 / 6323138 / AHP1 / Peroxiredoxin type-2 / 85 / 12 / S. cerevisiae
33 / 6322564 / SOD1 / Superoxide dismutase [Cu-Zn] / 70 / 6 / S. cerevisiae
35 / 6323613 / TSA1 / Thioredoxin peroxidase / 69 / 11 / S. cerevisiae
37 / 6323613 / TSA1 / Thioredoxin peroxidase / 53 / 9 / S. cerevisiae
52 / 6319546 / HSP26 / Heat shock protein 26 / 50 / 9 / S. cerevisiae
54 / 6319546 / HSP26 / Heat shock protein 26 / 50 / 8 / S. cerevisiae
55 / 6320742 / HSP31 / Heat shock protein 31 / 70 / 13 / S. cerevisiae
58 / 24158790 / CCP1 / Cytochrome c peroxidase / 57 / 17 / S. cerevisiae
28 / 6321401 / PNC1 / Nicotinamidase, Nicotine deamidase / 69 / 10 / S.cerevisiae
45 / 6322732 / SBA1 / Co-chaperone protein SBA1 / 32 / 6 / S. cerevisiae
Proteins related to other biological process
62 / 6324907 / VAT5 / Vacuolar proton pump subunit E / 60 / 14 / S. cerevisiae
10 / 642334 / COF1 / Cofilin / 60 / 7 / S. cerevisiae
50 / 14318474 / SEC53 / Phosphomannomutase / 61 / 15 / S. cerevisiae
11 / 6324945 / RPS12 / 40S ribosomal protein S12 / 31 / 5 / S.cerevisiae
22 / 6320510 / CPR5 / Cyclophilin D / 43 / 9 / S.cerevisiae
41 / 6320235 / PST2 / Protoplast secreted protein 2 [Precursor] / 69 / 10 / S. cerevisiae
46 / 6322707 / MRP8 / Uncharacterized protein (Ribosomal_MRP8) / 22 / 5 / S. cerevisiae
4 / 30749784 / Uncharacterized protein YHR087W / 21 / 3 / S. cerevisiae
42 / 50547479 / Similar to S. pombe GTP-binding protein ryh1 / 18 / 5 / Y. lipolytica
9 / 50422063 / Similar to S. cerevisiae Glutaredoxin / 38 / 5 / D. hansenii
24 / 50552039 / Similar to DEHA0A13277g Debaryomyces hansenii / 30 / 4 / Y. lipolytica
31 / 50556364 / Yarrowia lipolytica chromosome F / 32 / 3 / Y. lipolytica
Unknown
2 / 68486774 / Putative uncharacterized protein / 46 / 5 / C. albicans
7 / 126274424 / Predicted protein / 42 / 4 / P. stipitis
14 / 55419323 / Unknown / 35 / 4 / M. violaceum
48 / 154308992 / Unknown / 28 / 5 / B. fuckeliana
18 / 70986203 / Unknown / 24 / 4 / A. fumigatus
43 / 116508642 / Unknown / 39 / 5 / C. cinerea

Fig. S1. The changes in detected protein number of the industrial S. cerevisiae under throughout continuous (A) and batch/fed-batch (B) fermentation period by 2-DE analysis.