Electronic Supplementary Material (ESM): Plant Systematics and Evolution

A phenetic study of theEmilia coccineacomplex (Asteraceae, Senecioneae) in Africa

Ruvimbo J. Mapaya1,2* and Glynis V. Cron1

1 School of Animal, Plant and Environmental Sciences, University of the Witswatersrand, Private Bag 3, Wits 2050, South Africa

2 Department of Biological Sciences, Midlands State University, Private Bag 9055, Senga, Gweru, Zimbabwe

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Online Resource 1

Specimens of theE. coccineacomplex used in the phenetic study. Identification code used, locality information, altitude, and month of flowering are shown. Note: Specimens of E. caespitosa and E. vanmeeliiseen (!) with pubescent cypselas are indicated by asterisks (*)

ID Code / Herbarium specimen / Specimen locality / Altitude (m) / Month of flowering
Emilia caespitosa
9 / M. Reekmans 7465 (PRE) / Burundi, Ruyigi Prov.: Gasenye / 1750 / January
20 / H.U. Stauffer 998 (PRE) / DRC, Kivu Nord Distr. / 1470 / December
69 / A.W. Exell & F.A. Mendonҫa & H. Wild 948 (LISC) / Malawi, Ncheu Distr. / 1460 / March
70 / M. J. Masiye & E.J. Tawakali 241 (MO) / Malawi, Zomba Distr. / July
79 / M.O. Dale 117 (SRGH) / Mozambique, Manica / March
81 / G. Pope & T. Muller 574 (SRGH) / Mozambique, Zambezia Distr. / 500 / February
88 / G. Bouxin & M. Radoux 1602 (PRE) / Rwanda; Bugesera, plaine de Rutete / March
*93 / W. Kindeketa, C. Kayombo & G. S.Lazer 2503 (EA) / Tanzania, Dodoma: Mpwapwa Distr. / 1615 / May
*94 / Luke W.R.Q & P.A, M.A. Mwangoka, L.Festo & S.Bodine 10902 (EA) / Tanzania, RubehoMts: Ledingombe / 1700 / May
*95 / M.A. Mwangoka 932 (MA) / Tanzania, Tanga: Karogwe Distr. / 450–700 / October
*96 / I. R.Hizza 58 (MA) / Tanzania, Tanga: Muheza Distr. / 400 / November
97 / L. Festo, W. Wilbard & L.M. Charkes 1411 (MA) / Tanzania, Kagera: Bukoka Rural Distr. / 1162 / April
*98 / F. Mawi, A.J. Majengo, S.J. Salum & A.H. Samwe 96 (MO) / Tanzania, Singida Distr. / 1520 / March
*114 / P.K. Rwaburindore 4082 (MO) / Uganda, Kampala U4 District / 1200 / March
*115 / P.K. Rwaburindore 4770 (MO) / Uganda, Kampala U4 District / 1200 / April
127 / H.M. Biegel 4736 (LISC) / Zimbabwe, Mutare Distr. Mutare Heights / 1700 / January
128 / G. Pope 1612 (MO) / Zimbabwe, Mtoko Distr. Nyamahere hill / February
129 / G. Pope & T. Muller 1774 (SRGH) / Zimbabwe, Mutasa Distr. / 780 / November
132 / J.B. Phipps 547 (SRGH) / Zimbabwe, Mutare Distr. / 1036.3 / February
Emilia coccinea
1 / E.J. Mendes 45 (LISC) / Angola / September
2 / R. Monteiro, R.M. Santos & F. Murta 223 (LISC) / Angola, Cabinda, BucoZau / August
3 / J.M. TeixeiraA.M. Andrade 6395 (LISC) / Angola, Huambe Distr. Chianga / 1700 / June
4 / R. MonteiroF. Murta 1964 (LISC) / Angola, BIE, Cuemba / 1300 / August
5 / A. Borges 268 (LISC) / Angola, Huἰla, Lubango / June
6 / A.B. Machado 237 (LISC) / Angola, Alto Chicapa: Cascata de Rio Cuango-Muqùe / July
7 / A.W. Exell & F.A. Mendonҫa 1052 (LISC) / Angola, Luand: Rio Luachimo / 1300 / April
8 / C. Henriques 413 (PRE) / Angola / May
10 / M. Reekmans 8780 (EA) / Burundi, Bujumbura Prov.: Kabezi / 800 / April
19 / A.J.M. Leeuwenberg 8180 (MA) / Cameroon, Manengouba Mt. base near Nkongsamba / 1200 / August
37 / J.T. Carvalho 2089 (MA) / Equatorial Guinea: BIOCO / 450 / July
38 / J.T. Carvalho 4555 (MA) / Equatorial Guinea: BIOCO, Moca / 1150 / November
58 / H. Waaijenberg 2 (EA) / Kenya, Kilifi Distr. / 310 / August
59 / H.D. van Someren EA12255 (EA) / Kenya, Masai Distr. / January
60 / I. Masinde 54 (EA) / Kenya, Nairobi Distr. / June
61 / J. MacDonald 774 (EA) / Kenya, Nairobi Distr. / July
62 / P. Kucher 4376 (EA) / Kenya, K6: Masai Mara / 1800 / March
IDCode / Herbarium specimen / Specimen locality / Altitude (m) / Month of flowering
63 / P. Kucher & F. Msafiri 6256 (EA) / Kenya, K5: Masai Mara Game Reserve / 1650 / May
64 / W. P. Langridge 138 (EA) / Kenya, K1: Kilifi Distr. / August
65 / J.D. Briggs 34 (EA) / Kenya, K7: Gazi, Kwale Distr. / August
66 / P.R.O. Bally B8964 (EA) / Kenya, Nairobi Distr., Nairobi Govt. House / 1676.4 / June
67 / R.L. Davidson 444 (EA) / Kenya, Nyanza Prov., C.K. Distr., Sio Port / July
68 / J.C. Moomaus 840 (EA) / Kenya, Mariakani / 304.8 / September
71 / R.K. Brummitt 9238 (SRGH) / Malawi, Ncheu Distr. / 1550 / March
72 / R. Grosvenor & J. Renz 1048 (SRGH) / Malawi, Mzimba Distr. / March
73 / I.F. LaCroix 3086 (MO) / Malawi, Lilongwe Distr. / 1100 / July
74 / L.C. Leach & R. Rutherford-Smith 11061 (SRGH) / Malawi, Ncheu Distr. / 1006 / May
75 / I.H. Patel & J.L. Balaka 3672 (MO) / Malawi, Dedza Distr. / January
76 / P.O. Wiche N/595 (SRGH) / Malawi, Kule Range (Muso) / June
77 / J.L. Balaka & A.J. Salubeni 2163 (PRE) / Malawi, Lilongwe Distr. / September
78 / J.Pawek 6716 (PRE) / Malawi, Mzimba Distr. / 1676.4 / May
80 / P.C.M. Jansen & C. Boane 7888 (MO) / Mozambique, Niassa Prov. / 800 / February
82 / A.R. Torre & J. Paiva 10169 (LISC) / Mozambique, Niassa Prov. / 950 / January
83 / A.R. Torre & M.F. Correia 14325 (LISC) / Mozambique, Zambezi Distr. / 40 / February
84 / M.F. Correia 328 (LISC) / Mozambique, Tete Distr. / 900 / January
85 / L. Barbosa 910 (LISC) / Mozambique, Manica Prov. Sofala / January
86 / A. Gomes e. Sousa 4644 (PRE) / Mozambique, Manhambir Distr. / March
99 / P. Lovett & C. Kayombo 26 (MA) / Tanzania, Mbeya Distr. / 1800 / April
100 / P. Lovett, K. Sidwell & C. Kayombo 4065 (MA) / Tanzania, Mbeya Distr. / 1200 / February
101 / P. Lovett, K. Sidwell & C.J. Kayombo 4106 (MA) / Tanzania, Mbeya Distr. / 1300-1600 / February
102 / P.B. Phillipson, W.A. Rodgers & C. Rodgers 4993 (MA) / Tanzania, Arumeru Distr. / 1570 / May
103 / M. Ritchards 25261 (SRGH) / Tanzania, Mbulu/Singida Distr. / 1430 / January
104 / P.J. Madati 3 (EA) / Tanzania, Dar es Salaam / 30.48
105 / R. Polhill & S. Paulo 1338 (EA) / Tanzania, Iringa Distr. / 1250 / February
106 / G. P. Leedal 972 (EA) / Tanzania, Mbeya Distr. / January
107 / A. Bjornstad AB1359 (EA) / Tanzania, Iringa Distr. / 1200 / February
108 / E. Jaasund 2146 (EA) / Tanzania, Dar es Salaam / July
109 / P.J. Greenway & Kanuri12438 (EA) / Tanzania, Arusha Distr. / 1417 / March
110 / B.D. Burtt 4544 (EA) / Tanzania, Mfuwafwa Distr. / February
111 / D. Herlocker 366 (EA) / Tanzania, Ngorongoro Conservation area / March
116 / O. Kerfoot 5029 (EA) / Uganda, Karamoja District / 1371.6 / May
117 / H. Leippert 5088 (EA) / Uganda, Karamoja District / 1400 / October
118 / J.M. Lock 68/253 (EA) / Uganda, Ishasha, southern circuit / 975.4 / October
119 / R.B. Drummond 2569 (MO) / Zambia, Mwinilunga Distr. / November
120 / Unknown 2392 (UZL) / Zambia, Kipili Distr. / 975 / February
121 / Unknown 1514 (UZL) / Zambia, Eastern Prov.
122 / S.D. Prince 194 (UZL) / Zambia, Luangwa valley / 610 / February
123 / L.. Silungwe 272 (UZL) / Zambia, Abercorn / February
124 / A.A. Bullock 2130 (LISC) / Zambia, Abercorn near Lake Chila / December
125 / N.K.B. Robson 1073 (PRE) / Zambia, Central Prov., Fort Manning / 1250 / January
ID Code / Herbarium specimen / Specimen locality / Altitude (m) / Month of flowering
126 / A. Angus 2195 (PRE) / Zambia, Northern Prov. / March
130 / R.D.A. Bayliss 10117 (MO) / Zimbabwe, Mazowe, Christon Bank / 1219 / March
131 / T. Muller & R. D. Kelly 1188 (SRGH) / Zimbabwe, Chimanimani Distr. / 305 / May
133 / J.B. Phipps 547 (SRGH) / Zimbabwe, Mutare Distr. / 1036 / February
133 / H. Wild 3592 (SRGH) / Zimbabwe, Chimanimani Distr. / 1006 / October
134 / A.J. Jeague 16 (PRE) / Zimbabwe, Mutare Distr.
E. jeffreyana
11 / M. Reekmans 483 (BR) / Burundi, Bubanza, Musigate / 1300 / April
12 / M. Reeekmans 8530 (BR) / Burundi, Muramwaya Prov. / 2200 / December
13 / J. Lewalle 3122 (BR) / Burundi, Bujumbura Prov. / 780 / November
21 / Meurillon 853 (BR) / DRC, Kivu Prov., Birava / 1500 / April
22 / Meurillon 855 (BR) / DRC, Kivu Prov., Birava / 1500 / April
23 / Y. & T. Ankei 79/0364 (BR) / DRC, Ubwari peninsula / 850 / November
24 / Boutakoff34 (BR) / DRC, Curette de Nya-Gezi / 1700 –1900 / Sept. / Oct
25 / B. Bytebier & W.R.Q Luke 2750 (BR) / DRC, Maniema Prov., Namoya / 960 / April
26 / A. Gilon 283 (BR) / DRC, Kivu Prov., Environs de Luberizi / December
89 / P. Auquier 3623(BR) / Rwanda, Cyangugu / 1500 / August
90 / G. Bouxin 24 (BR) / Rwanda, Cyangugu / 1970 / January
91 / G. Troupin 11961 (BR) / Rwanda, Shangugu / 2000 / March
92 / G. Troupin 9850 (BR) / Rwanda, Shangugu / 2000 / March
E. lisowskiana
39 / E. Aedo, R.P. VisoM. Velayos 5394 (MA) / Equatorial Guinea, Centro Sur: pr. Acurenam / 575 / March
40 / S. Castroviejo 9198SC (MA) / Equatorial Guinea, Miboman, a 22 km de Bata / June
41 / P. Barberá, F. Cabezas, P. Esono & M. de la Estrella 1342 (MA) / Equatorial Guinea, KieNtem: Nfuiñ / 630 / December
42 / M. Fero & P. Esono 118 (MA) / Equatorial Guinea, KieNtem: Akam II / March
43 / J.Lejoly 99/064 (MA) / Equatorial Guinea, Parc Nat. de Monte Alen / 1120 / January
44 / R.P.Viso 2398 (MA) / Equatorial Guinea, Centro Sur: Niefang, Bindeng / April
45 / F. Poo 167 (MA) / Equatorial Guinea / December
E. emilioides
15 / W. de Wilde 4404 (BR) / Cameroon, Ngaoundéré / 1200 / December
16 / W. de Wilde 4640 (BR) / Cameroon, Ngaoundéré / 1200 / December
17 / W. de Wilde 4257 (BR) / Cameroon, Ngaoundéré / 1200 / November
51 / I. Friis, A. Hounde & K. Jacobsen 601 (BR) / Ethiopia, Kaffa Prov., Kochi / 1740 / December
52 / W. de Wilde 7493 (BR) / Ethiopia, Addis Ababa., 200km SW along road to Jima / 2000 / August
53 / I. Friis, G. Aweke, F. Rasmussen 2061 (BR) / Ethiopia, Kaffa Prov., Kochi / 1690 / January
54 / W.J.J.O. de Wilde & B.E.E. de Wilde-Duyfjes 6225 (BR) / Ethiopia, Addis Ababa., between Sire & Bako / 2100 / April
55 / W.J.J.O. de Wilde & B.E.E. de Wilde-Duyfjes 6837 (BR) / Ethiopia, Addis Ababa, 70km NE of Jimma / 2000 / June
E. praetermissa
18 / J.J.F.E. de Wilde 8342 (MA) / Cameroon, Campo / 20 / June
46 / R. P.Viso 1404 (MA) / Equatorial Guinea, Centro Sur: Bata: Asonga / July
47 / R. P.Viso 2479 (MA) / Equatorial Guinea, Litoral: Rio Campo / April
48 / R. P.Viso 1897 (MA) / Equatorial Guinea, Litoral: Corisco / March
ID Code / Herbarium specimen / Specimen locality / Altitude (m) / Month of flowering
49 / F.J. Cabezas, TelleriaM Velayos 9921 (MA) / Equatorial Guinea, Litoral: Corisco / December
50 / F.Casas 11675 (MA) / Equatorial Guinea, Biocco: Malabo-Riaba / 400 / February
56 / J. Dibata 1060 (MO) / Gabon, Okala, N of Libreville / August
57 / F.J. Breteler 5290 (EA) / Ivory Coast, Abidjan-Aboisso road / November
87 / E. Pilz 2116 (PRE) / Nigeria, State: Oyo / September
E. subscaposa
14 / M. Reekmans374(BR) / Burundi, Bujumbura, Randa / 850 / April
27 / J.J. Symoens 1792 (BR) / DRC, Kabalagafuiesd'Uvira / 1030 / November
28 / U. Kinet 274 (BR) / DRC, Kivu Prov., Uvira / 776 / April
29 / R. Germain 5518 (BR) / DRC, Kabunambo, Plaine de la Ruzizi / January
30 / U. Kinet 171 (BR) / DRC, Kivu Prov., Uvira / 774 / March
E. vanmeelii
*31 / S. Lisowski 85626 (BR) / DRC, Haut-Shaba / January
*32 / A.Bodenghien 340 (BR) / DRC, Kapanitono, Shaba / 1400 / January
33 / L. Dubois 1245 (BR) / DRC, Moba / 1400 / July
34 / G.F. de Witte 428 (BR) / DRC / June
*35 / P. Bamps & F. Malaisse8506(BR) / DRC, Mitwaba–Manono / 800 / February
*36 / Luxen 30 (BR) / DRC, Katubilwa / May
*112 / P. Kuchar 23261 (MO) / Tanzania, Singida Distr. / 1570 / March
*113 / N. A. Mwangulango 858 (MO) / Tanzania, (T4): Rukwa: Mpanda Distr. / 1370 / May

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