Title: Efficacy of Management Tools for Fusarium Root & Crown Rot.
Protocol #: 13-016Draft 1
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Objective:Determine efficacy of new active ingredient formulations and new biopesticides for managing root, crown and stem rots of ornamental plants caused by Fusarium species.
Experimental Design:
Plot Size: Must be adequate to reflect actual use conditions.
Replicates: Minimum of 4.
Application Instructions:For 2012, soil or plant material is to be inoculated with Fusarium species prior to fungicide treatment. A number of different application methods can be employed based on the disease system studied. Application rates and intervals follow in tables below for foliar, drench, corm/root dip, top dress, and soil incorporation application methods. Multiple application types may be utilized in the same experiment, but rates must be used consistent with the different methods.. Applications should be made using application equipment consistent with conventional commercial equipment. Calibrate application equipment prior to application. Read available labels before making applications. Avoid treating plants under unusually extreme environmental conditions.
Target Species:TBD. Contact your regional coordinator for specifics.
Plant Hosts:Use a plant host suitable for target species, recording species and variety used.
Use Site: May be greenhouse, field container or field in-ground. Please specify in final report.
Evaluations: Record disease severity and incidence 0, 14, and 28 days after application for herbaceous plant material. For woody plant material, record disease severity and incidence 0, 14, 28, 42 and 56 days after initial application. Record plant height & width at initial and final evaluations only. Record phytotoxicity at each rating date on a scale of 0 to 10 (0 = no phytotoxicity; 10 = complete kill). If phytotoxicity is observed in treated plants, take pictures comparing treated and untreated plant material.
Recordkeeping:
All operations, data and observations appropriate to this study should be recorded. It is helpful to review the Ornamental Horticulture Research Report Form in advance. Keep detailed records of weather conditions including temperature, precipitation and/or irrigation, and relative humidity with a minimum of high, low and average daily temperatures. Other information to record includes soil-type or soil-less media, application equipment, irrigation (type & frequency), liner size, plant height & width, and plant growth stage at application and data collection dates.Content of reports should be sufficient to fully understand how the experiment was conducted.
Photographs often illustrate experimental design, site conditions, and impacts of treatments very well. It is encouraged to include a picture or two of the greenhouse, field or landscape where the experiment is sited. It is highly encouraged that pictures illustrating treatment effects are taken if and when these impacts are visually apparent.
If different application methods or evaluations are made, please clearly specify differences in final report and explain reason for change.
Treatments:
See table on the following page.
Foliar Treatments:
Priority / # / Product / Foliar Rates(per 100 gal) / Reapplication Interval / Special Application Instructions (Foliar)
A / 1 / Acibenzolar / 0.5 oz / 7 d / Must be applied twice before exposing to pathogen or inoculation
2 / 0.75 oz
3 / RootShield Plus BW240 WP (Trichoderma harzianum T-22 + Trichoderma virens G-41) / 8 oz
4 / Cg100 / 38.4 fl oz
5 / Palladium (cyprodinil and fludioxinil) / 6 fl oz / 7 – 14 d / The determination of application frequency can be determined by the researcher based on their project.
6 / SP2169 / 12.3 fl oz
7 / Tourney (metconazole) / 2 oz
8 / 4oz
9 / Trinity (triticonazole) / 8 fl oz
10 / 12 fl oz
Standards / 11 / Standard Control / See below / See below
12 / Untreated Uninoculated / -- / --
13 / Untreated Inoculated / -- / --
Standards / a / 3336 (thiophanate-methyl) / 16 oz
b / Medallion (fludioxonil) / 4 oz / 7 – 14 d / The determination of application frequency can be determined by the researcher based on their project.
c / Terraguard (triflumizole)
B / Acibenzolar + Heritage / 0.5 oz + 1.8 oz
0.75 oz + 2.7 oz
Compass O (trifloxystrobin)
DisArm / 4 fl oz
Heritage (azoxystrobin) / 1.8 oz
Insignia (pyraclostrobin)
Pageant 38WG / 12 oz / 14 day / Apply with sufficient coverage for foliar disease protection prior to first sign of infection.
Torque (tebuconazole) / 10 fl oz
Directed Spray Treatments (to crown or base of plant):
Priority / # / Product / Directed Spray Rates(per 100 gal) / Reapplication Interval / Special Application Instructions (Directed Spray)
A / 1 / Acibenzolar / 0.5 oz / 7 d / Must be applied twice before exposing to pathogen or inoculation
2 / 0.75 oz
3 / RootShield Plus BW240 WP (Trichoderma harzianum T-22 + Trichoderma virens G-41) / 8 oz
4 / Cg100 / 38.4 fl oz
5 / Palladium (cyprodinil and fludioxinil) / 6 fl oz / 7 – 14 d / The determination of application frequency can be determined by the researcher based on their project.
6 / SP2169 / 12.3 fl oz
7 / Tourney (metconazole) / 2 oz
8 / 4oz
9 / Trinity (triticonazole) / 8 fl oz
10 / 12 fl oz
Standards / 11 / Standard Control / See below / See below
12 / Untreated Uninoculated / -- / --
13 / Untreated Inoculated / -- / --
Standards / a / 3336 (thiophanate-methyl) / 16 oz
b / Medallion (fludioxonil) / 4 oz / 7 – 14 d / The determination of application frequency can be determined by the researcher based on their project.
c / Terraguard (triflumizole)
B / Acibenzolar + Heritage / 0.5 oz + 1.8 oz / 7 – 14 d / The determination of application frequency can be determined by the researcher based on their project.
0.75 oz + 2.7 oz
Compass O (trifloxystrobin)
DisArm / 4 fl oz
Flagship 25WG / 6 oz / 7 – 14 d / The determination of application frequency can be determined by the researcher based on their project.
Flagship 25WG + Heritage / 6 oz + 4 oz / 7 – 14 d / The determination of application frequency can be determined by the researcher based on their project.
Heritage (azoxystrobin) / 1.8 oz / 7 – 14 d / The determination of application frequency can be determined by the researcher based on their project.
Insignia (pyraclostrobin)
Pageant 38WG / 12 oz / 14 day / Apply with sufficient coverage for foliar disease protection prior to first sign of infection.
Torque (tebuconazole) / 10 fl oz
Drench Application Treatments:
Priority / # / Product / Drench Rates(per 100 gal) / Reapplication Interval / Special Application Instructions (Soil Drench)
A / 1 / Acibenzolar / 0.125 oz / 21 days / Apply as a drench 2 weeks prior to inoculations
2 / 0.25 oz
3 / RootShield Plus BW240 WP (Trichoderma harzianum T-22 + Trichoderma virens G-41) / 6 oz / ½ rate at 10 to 12 weeks / Apply material 72 hours before pathogen inoculation or dip plants before planting into inoculated soil
4 / Cg100 / 38.4 oz
5 / Palladium (cyprodinil and fludioxinil) / 6 oz / 21 d
6 / SP2169 / 12.3 oz per 100 gal
Optional: 24.6 oz per 100 gal
7 / Tourney (metconazole) / 2 oz / Apply as a drench
8 / 4 oz
9 / Trinity (triticonazole) / 8 fl oz / Apply as a drench
10 / 12 fl oz
Standards / 11 / Standard Control / See below / See below / See below
12 / Untreated Uninoculated / -- / -- / --
13 / Untreated Inoculated / -- / -- / --
Standards / a / 3336 (thiophanate-methyl)
b / Medallion (fludioxonil) / 2 oz / 21 days / Apply as a drench to thoroughly wet ¾ of the root zone
c / Terraguard (triflumizole)
B / Acibenzolar + Heritage / 0.125oz + 0.45oz / 1-2 application – 28 day / The determination of application frequency can be determined by the researcher based on their project. I realize this will vary.Apply at 1-2pts/sqft
Acibenzolar + Heritage / 0.25oz + 0.9oz / 2 application – 28 day / The determination of application frequency can be determined by the researcher based on their project.
Compass O (trifloxystrobin)
DisArm / 4 oz
Flagship 25WG / 6 oz / 2 applications – 28 day
Flagship 25WG + Heritage / 6 oz + 0.9 oz / 2 applications – 28 day / The application frequency can be determined by the researcher based on their project. Apply at 1-2 pts per sqft.
Flagship 25 WG + Hurricane / 6 oz + 1.5 oz / 1 application – 28 day
Heritage (azoxystrobin) / 0.9 oz / 28 days / Drench to thoroughly wet ¾ of the root zone
Hurricane (fludioxonil + mefonaxam) / 1.5 oz
Insignia (pyraclostrobin) / 6.1 fl oz
Pageant 38WG / 12 oz / 14 day / Apply with sufficient coverage for foliar disease protection prior to first sign of infection.
Torque (tebuconazole) / 10 fl oz
Corm Dip Treatments:
Priority / # / Product / Corm Dip Rates(per 100 gal) / Reapplication Interval / Special Application Instructions (Corm Dip)
A / 1 / Acibenzolar / 0.125 oz / none
2 / 0.25 oz
3 / RootShield Plus BW240 WP (Trichoderma harzianum T-22 + Trichoderma virens G-41) / 6 oz / ½ rate at 10 to 12 weeks / Apply material 72 hours before pathogen inoculation or dip plants before planting into inoculated soil
4 / Cg100 / 38.4 oz
5 / Palladium (cyprodinil and fludioxinil) / 6 oz
6 / SP2169 / 12.3 oz per 100 gal
Optional: 24.6 oz per 100 gal
7 / Tourney (metconazole) / 2 oz
8 / 4oz
Standards / 9 / Standard Control / See below / See below / See below
10 / Untreated Uninoculated / -- / -- / --
11 / Untreated Inoculated / -- / -- / --
Standards / a / 3336 (thiophanate-methyl)
b / Medallion (fludioxonil) / 2 oz / none
c / Terraguard (triflumizole)
B / Acibenzolar + Heritage / 0.125 oz + 0.45 oz / none
0.25 oz + 0.9 oz
0.5 oz + 1.8 oz
Compass O (trifloxystrobin)
DisArm / 4 oz
Heritage (azoxystrobin) / 4.0 oz / none
0.9 oz
1.8 oz
Torque (tebuconazole) / 10 fl oz
Top Dress Treatments (Application to soil surface either spray or granular broadcast, followed by irrigation):
Priority / # / Product / Top Dress Rates(per 1000 sq ft) / Reapplication Interval / Special Application Instructions (Soil Drench)
A / 1 / Acibenzolar / (0.08 g) / 21 days / Topdress rate given in parenthesis is based on a drench rate of 0.125 and 0.25 with a 1 pt/sqft application volume.
2 / (0.16 g)
3 / RootShield Plus BW240 WP (Trichoderma harzianum T-22 + Trichoderma virens G-41) / 32 oz per acre / ½ rate at 10 to 12 weeks / Apply material 72 hours before pathogen inoculation or dip plants before planting into inoculated soil
4 / Cg100 / 26.0 ml
5 / Palladium (cyprodinil and fludioxinil) / 1oz
Container Rate
(5oz ) / Topdress rate given in (parenthesis) is based on a drench rate of 4oz /100gal with a 1 pt/sqft application volume. This rate should be used if the trial is for container grown plants, otherwise use other rate.
6 / SP2169 / 8.35 ml
7 / Tourney (metconazole) / 2.8 g
8 / 5.6 g
9 / Trinity (triticonazole) / 5.43 ml
10 / 8.14 ml
Standards / 11 / Standard Control / See below / See below
12 / Untreated Uninoculated / -- / --
13 / Untreated Inoculated / -- / --
Standards / a / 3336 (thiophanate-methyl) / 10.8 ml
b / Medallion (fludioxonil) / 0.75 oz per 1000 sq ft
Container Rate
(2.5oz) / 21 days / This top rate is based on label use limit for outdoor production assuming 2 applications allowed per year.
The rate given in (parenthesis) is based on a drench rate of 2oz /100gal with a 1 pt/sqft application volume. This rate should be used if the trial is for container grown plants, otherwise use other rate.
c / Terraguard (triflumizole)
B / Acibenzolar + Heritage / (0.08g + 1oz) / Topdress rate given in parenthesis is based on a drench rate of 0.125 and 0.9oz/100 gal with a 1 pt/sqft application volume.
Compass O (trifloxystrobin) / 2.71 ml
DisArm / 2.71 ml
Flagship 25WG / 0.55 oz/1000 linear ft (3 ft row spacing)
Flagship 25WG + Heritage / 0.4 oz + 0.4 oz
Container Rate
(7.5oz + 1oz) / This top rate is based on label use limit for outdoor production assuming 2 applications allowed per year.
The rate given in (parenthesis) is based on a drench rate of 6oz +0.9oz /100gal with a 1 pt/sqft application volume. This rate should be used if the trial is for container grown plants, otherwise use other rate..
Flagship 0.22G / 44 oz (2.76 lb) per 1000 ft
Flagship 0.22G + Heritage / 44 oz (2.76 lb) + 0.4 oz per 1000 ft / Field application rate
Heritage (azoxystrobin) / 0.4oz
Container rate
(1 oz) / This top rate is based on label use for outdoor production as a banded treatment.
The rate given in (parenthesis) is based on a drench rate of 0.9oz /100gal with a 1 pt/sqft application volume. This rate should be used if the trial is for container grown plants, otherwise use other rate.
Insignia (pyraclostrobin)
Pageant 38WG / 14 day
Torque (tebuconazole) / 10 ml per 100 gal delivering 0.24 ml product per 11.8 sq ft.
Title: Efficacy of Management Tools for Fusarium Root & Crown Rot.
Protocol #: 13-016Draft 1
p 1 of 10
Sources for Products:
Product / Contact / Phone / Email3336 / Cleary, Rick Fletcher / 800-524-1662 /
Acibenzolar / Syngenta, Steve Cosky / 336-632-7148 /
Cg100 / AMVAC, Ned French / 949-260-1212 /
DisArm / OHP, Jeff Dobbs / 770-992-0121 /
Compass 0 / OHP, Jeff Dobbs / 770-992-0121 /
Flagship / Syngenta, Steve Cosky / 336-632-7148 /
Heritage / Syngenta, Steve Cosky / 336-632-7148 /
Hurricane / Syngenta, Steve Cosky / 336-632-7148 /
Insignia / BASF, Kathie Kalmowitz / 919-270-4592 /
Medallion / Syngenta, Steve Cosky / 336-632-7148 /
Pageant / BASF, Kathie Kalmowitz / 919-270-4592 /
Palladium / Syngenta, Steve Cosky / 336-632-7148 /
RootShield Plus / BioWorks, Matt Krause / 800-877-9443 x 335 /
SP2169 / SePRO, Jessica Koczan / 252-391-8382 /
Torque / Cleary, Rick Fletcher / 800-524-1662 /
Tourney / Valent, Joe Chamberlin / 770-985-0303 /
Trinity / BASF, Kathie Kalmowitz / 919-270-4592 /
Reports:
Reports are due 60 days after last data collection date.
Submit reports electronically on the standard IR-4 Ornamental Horticulture Research Report Form. The basic report can be sent in MS Word, the recordkeeping information as pdf or other electronic documents, the raw data in MS Excel or other suitable program such as ARM, and photographs can be submitted as picture embedded in the report or as separate jpg, bmp, or tiff files.
Please direct questions to:
Cristi Palmer, IR-4 HQ, Rutgers University, 500 College Road East, Suite 201W, Princeton, NJ 08540, Phone 732-932-9575 x4629,
OR
Ely Vea, 308 Aston Forest Lane, Crownsville, MD 21032, Phone & FAX#: 410-923-4880, E-mail: .
Draft Date: 9/21/2012
Revised By: CLP