SupplementaryTable 1 Renal eicosanoids in normal (CD1-+/+) and pcy(CD1-pcy/pcy) mice

15 Days¶ / 30 Days / 60 Days
Eicosanoids / Normal / pcy / Normal / pcy / Normal / pcy
COX products
6-keto-PGF1α / 283.4± 58.4 / 522.3 ± 77.7 / 90.3 ± 19.3 / 446.9 ± 139.6** / 131.6 ± 22.1 / 589.1 ± 102.2 ***
TXB2 / 18.0 ± 2.4 / 26.1 ± 3.2 / 11.5 ± 1.9 / 32.2 ± 7.9 ** / 27.2 ± 2.8 / 67.3 ± 16.5 *
PGF2α / 291.5 ± 30.0 / 331.8 ± 19.9 / 310.1 ± 84.5 / 418.2 ± 101.9 / 233.8 ± 40.5 / 586.3 ± 101.4 **
PGE2 / 203.8 ± 43.1 / 295.6 ± 60.1 / 124.0 ± 18.3 / 427.5 ± 127.1 ** / 150.8 ± 30.5 / 280.7 ± 37.8 *
PGD2 / 126.5 ± 12.5 / 125.0 ± 25.5 / 31.6 ± 7.6 / 53.9 ± 14.6 / 28.6 ± 9.4 / 59.5 ± 11.7
LOX products
5-HETE / 520.0 ± 50.6 / 648.3 ± 129.6 / 1071.5 ± 399.8 / 458.2 ± 146.1 * / 1751.5 ± 419.2 / 598.8 ± 72.6 *
8-HETE / 165.7 ± 16.9 / 206.3 ± 29.5 / 298.4 ± 127.7 / 108.6 ± 17.8 / 590.7 ± 158.4 / 209.1 ± 28.8 **
9-HETE / 93.7 ± 10.6 / 99.0 ± 15.2 / 184.4 ± 70.1 / 48.8 ± 11.4 ** / 305.4 ± 67.4 / 112.0 ± 18.4 *
11-HETE / 1085.9 ± 90.4 / 1217.0 ± 161.3 / 1490.3 ± 443.1 / 1172.9 ± 364.1 / 2423.2 ± 493.5 / 1549.9 ± 121.7
12-HETE / 1153.0 ± 132.2 / 1227.9 ± 209.9 / 990.7 ± 205.2 / 800.6 ± 240.0 / 2561.7 ± 267.9 / 2478.4 ± 699.3
15-HETE / 274.6 ± 26.3 / 293.9 ± 42.6 / 479.3 ± 103.1 / 315.6 ± 76.2 / 1209.4 ± 325.0 / 492.2 ± 106.3 *
9-HODE / 1589.7 ± 185.5 / 1638.6 ± 387.0 / 2140.2 ± 667.2 / 1340.9 ± 354.8 / 6216.4 ± 1357.3 / 2470.4 ± 257.7 *
13-HODE / 1698.2 ± 119.1 / 1864.2 ± 351.6 / 2366.1 ± 696.1 / 1468.4 ± 331.0 / 7214.2 ± 1509.6 / 2790.0 ± 279.0 **

¶ TxB2 and all LOX products are highest at 60 compared to 15 and 30 day values (P<0.05). PGD2 values at 15 days are higher than 30 and 60 day values (P<0.05).

*P<0.05, **P<0.01, ***P<0.001: Significantly different from corresponding value for mice at same age.

Values are expressed as mean ng perkidney± SE, n=6

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