Dr. Vaibhav Mishra

Division of Pharmacology

CSIR-Central Drug Research Institute,

Lucknow- 226031, India.

Mob. no.:+91-9450332710

Email: ,

Educational Qualification:

  • Ph.D.-Completed in Applied Microbiology under the Jointsupervision ofDr.ManojkumarBarthwal,Senior Scientist, Pharmacology Division, CSIR-Central Drug Research Institute, Lucknow, India and Ex-Prof. H. P. Pandey, Biochemistry Department, Banaras Hindu University, Varanasi, India. And under co-supervision ofDr. GautamPalit Ex-Head & Chief Scientist, Pharmacology Division, CSIR-CDRI, Lucknow, India.

Thesis title “Evaluation of gastro protective effect of natural products & elucidation of its molecular mechanism”.

  • Ph.D. Course work, 2011-2012- Department of Biochemistry, BHU, Varanasiwith 89% marks.
  • Master of Science (Microbiology) - 2007, VBSP University, Jaunpur, with 68% marks.
  • Bachelor of Science (Biology) -2002, T. D. S. P.G. College Affiliated to Veer Bahadur Singh, Purvanchal University Jaunpur (U.P.), with 61% marks.
  • Intermediate, 1999, (U.P. Board) with 64% marks.
  • High School, 1997, (U.P. Board) with 65% marks.

Research Experiences:

Ph.D. Student- July 2011- 2014, Division of Pharmacology, Central Drug Research Institute, Lucknow, India.

Research summary:

The objective of my dissertation is to explore various natural products for their anti-ulcer activities and to identify the active lead molecules, in a process to identify and develop candidate drug for the prevention of Peptic Ulcer diseases. The respective herbal product that was found satisfactory were further fractionated and finally analyzed for the active component responsible for the anti-ulcer activity of its parent herbal extract. I have identified various natural products such asTerminaliachebula (TC), its active constituent chebulinic acid, Peganine hydrochloride compound isolated from the seeds of Peganumharmala (PH), 6-Hydroxy loganin and Arbortristoside-A active constituent isolated from the seeds of Nyctanthesarbortristis (NA)which exhibits high degree of protection in various rodents model of gastric ulcerationeither through their anti-secretory or cytoprotective properties. Apart from invivo screening of natural compounds in ulcer models, I extensively studied the changes in gastric juice components; blood plasma gastrin level, proton pump activity in gastric microsomes and PGE2 levels in ulcerated mucosa of rats to confirm the mechanism of their action. In addition to ulcer protective activities, compoundsisolated from natural products were also tested for their ulcer healing properties in chronic gastric ulcer model in rodents. The healing parameters such as reduced ulcer crater size, over expression of growth factors (VEGF, EGF) and COX-2 levels were examined to compare the healing potential of herbal compounds with respect to reference drug.

Project Fellow- Jun2008- April 2011, Division of Pharmacology, CSIR-Central Drug Research Institute, Lucknow, under supervision of Dr. GautamPalit on project titled “Discovery, Development and Commercialization of new Bioactive and Traditional preparation for anti-ulcer and anti-anxiety activity from plant source”.

Research summary:

Anti-anxiety (Neuroscience)

  • Screening of natural compounds like Dysoxylembinectriferum bark extract and its active constituent Rohitukine for anti-anxiety activity in anxiety models likeelevated plus maze, vogel conflict test and hole pock test inswiss mice and comparison with reference anti-anxiety drug diazepam.

Anti-Depression (Neuroscience)

  • Screening of natural products and its active constituent for anti-depressant activity in depression models likeforced swim test and tail suspension test inswiss mice and comparison with reference anti-depressant drugfluxotene.

Anti-Psychotic (Neuroscience)

  • Screening of natural compounds for anti-psychotic activity in ketamine induced psychosis models in swiss mice and comparison with reference anti-psychotic drug haloperidol, clozapine and reserpine.

Anti-ulcer (Gastroenterology)

  • Screening of natural products for anti-ulcer activityin acute models including cold restraint, alcohol, aspirinand pyloric ligation induced gastric ulcer models in rats and comparison with reference anti-ulcer drugs.
  • Ulcer healing activity was assessed in acetic acid induced chronic gastric ulcer model in rats and compared with reference anti-ulcer drug.
  • Evaluation of biochemical activity of extract by measuring free, total acidity, peptic activity and mucin secretion.
  • Activity guided fractionation of extract for anti-ulcer activity.
  • Effect of extract and fraction on free radical and anti-oxidant enzyme studies.
  • Extent of healing was checked by histopathological analysis.
  • Western blot analysis of COX-2 protein and prostaglandin of pure active compounds.
  • To identify lead molecules Gedunine and Photogedunine isolated from plants like Xylocarpusgranatumfruit for the development of anti-ulcer drug in future.

M.Sc. Dessertation-14th Februaryto 14th June2007, CSIR-Industrial Toxicology Research Centre, Lucknow. In the lab of Dr. B.N.Paul Head of Immunobiology Department on project titled “To Study the Effect of STAT3 Gene Silencing in Normal Mice by STAT3 si-RNA Transfection”.

M.Sc. Training-“Isolation of Micro organism and its Characterization”in Veer Bahadur Singh Purvanchal UniversityJaunpurunder supervision of Dr. S.P.Tiwari, Head of Microbiology, Faculty of Science,Jaunpur.

Research summary:

  • Preparation of Seed and production of media for culturing bacteria (antibiotic producing).
  • To estimate the concentration of Protein using Bioassay method.
  • Strain Improvement using U.V. mutation.
  • Estimation of secondary metabolite by using fermented Pilot plant.

Awards and Honours

●Recipient of Senior Research Fellowship (April 2011-to April 2014) from Council of Scientific Industrial Research (CSIR), Govt. of India.

●Recipient of Senior Research Fellowship (2010) from Indian Council of Medical Research (ICMR), Govt. of India.

●Three times CRET Qualified conducted by BHU.

●3rdtopper in M.Sc. Applied Microbiology, Veer Bahadur Singh Purvanchal University, Jaunpur (2007).

●2nd topper in Junior high school, BRP Inter College, Jaunpur (1995).

Publications

Research articles in National and International Journals:

  1. Neetu Singh, ShishirShrivastva, Sunil Kumar Mishra, PoonamDharmani, VaibhavMishra and GautamPalit. Gedunin and Photogedunin of Xylocarpusgranatum show significant antisecretory effects and protect the gastric mucosa of peptic ulcer in rats. Phytomed 2010, 17, Issues 8-9, 569-574. (Impact factor 3.3)
  2. NailaRasheed, Neetu Singh, Pratibha Singh, VaibhavMishra, MohtashimLohani, GautamPalit. Differential response of A68930 and sulpiride in stress-induced gastric ulcers in rats. Europ J Pharmacol 2010, 643, 121-128.(Impact factor 2.5)
  3. Onasanwo SAdetunji, Neetu Singh, Olaleyea SB, VaibhavMishra and GautamPalit. Antiulcer and antioxidant activities of Hedrantherabarteri {(Hook F.) Pichon} with possible involvement of H+, K+ ATPase inhibitory activity. Indian J Med Res 2010,132, 442-449.(Impact factor 2.0)
  4. VaibhavMishra,ManaliAgrawal,Samuel AdetunjiOnasanwo,GauravMadhur, PreetiRastogi, Haushila Prasad Pandey, GautamPalit,TadigoppulaNarender. Anti-secretaryand cyto-protective effects of chebulinic acid isolated from the fruits of Terminaliachebula on gastric ulcers. Phytomed2013, 20, 506–511.(Impact factor 3.3)
  5. Amit K. Gupta, KanikaVarshney,Neetu Singh, VaibhavMishra, MridulaSaxena, GautamPalit and Anil K. Saxena.Identification of Novel Amino Acid Derived CCK-2R AntagonistsPotential Antiulcer Agent: Homology Modeling, Design, Synthesis,and Pharmacology.J Chem Information and Modeling. 2013, 53, 173-87.(Impact factor 4.65)
  6. VaibhavMishra, Asthashukla, Sukanyapandeti, Manoj Kumar Barthwal, Haushila Prasad Pandey, GautamPalit and TadigoppulaNarender. Arbortristoside-A and 7-O-Trans-cinnamoyl- 6β- hydroxyloganin isolated from Nyctanthesarbortristis possess anti-ulcerogenic and ulcer-healing properties.Phytomed2013, 20, 1055–1063.(Impact factor 3.3)
  7. Vinay Kumar Singh, VaibhavMishra,SriniwasTiwari, TanvirKhaliq, Manoj Kumar Barthwal, Haushila Prasad Pandey,GautamPalit and TadigoppulaNarender.Anti-secretory and cyto-protective effects of Peganine hydrochloride isolated from the seeds of Peganumharmala on gastric ulcers. Phytomed2013, 20,1180–1185.(Impact factor 3.3)
  8. Samuel A. Onasanwo, Taiwo O. Oyagbemi, Ademola A. Oyagbemi, VaibhavMishra. Antihistaminic potentials of Parquetinanigrescens in laboratory rodents. Journal of Natural Pharmaceuticals, 2013, Issue 2, 93-98.
  9. RohitSingh,VaibhavMishra,SukanyaPandeti, GautamPalit, Manoj K. Barthwal, Haushila Prasad Pandey and TadigoppulaNarender.Cytoprotective and Anti-secretory Effects of Azadiradione Isolated from the Seeds of Azadirachtaindica (neem) on Gastric Ulcers in Rat Models.Phytother Res 2015,29,910-916. (Impact factor 2.66)
  10. Koneni V. Sashidhara, SrinivasaRaoAvula, VaibhavMishra, Neetu Singh Gopal Reddy palnati, YashpalS.Chhonker, Priyanka Swami, R.S. Bhatta, Gautampalit.Discovery and synthesis of Quinoline-based Chalcones as Potential Anti-Ulcer Agents.Europ Med Chem2015, 89, 238-253(Impact factor 3.4)

Abstract Publication:

  1. VaibhavMishra,VijaiLakshmi, Haushila Prasad Pandeyand GautamPalit. Anti-ulcer activity of andrographaloide: possible involvement of H+, K+ ATPase activityJ. of Biotechnol and Biomaterials. 2012, vol-2 issue -6 pp 327.

Papers/ Posters/ Invited Speakers presented in Conferences/ Workshops:

  1. VaibhavMishra and GautamPalit“Medicinal plantHedrantherabarteri {(Hook F.) Pichon} shows protection in gastric ulcer with involvement of H+, K+ ATPase activity”, In International conference on Emerging Trends in Biotechnology, at Banaras Hindu University, Varanasi, India. 4th – 6th December 2009.
  2. NeeleshGanguar,VaibhavMishra,Seema Singh,VijaiLakshmiand GautamPalit. “Anti anxiety effect of Rohitukine isolated fromDysoxylum Binectariferumin swiss mice ”, InIndian Academy of Neurosciences (IAN), at New Delhi, India. 12th – 14th December 2011.
  3. VaibhavMishra,KushalKesarwani, Neetu Singh,Haushila Prasad Pandey,VijaiLakshmiand GautamPalit. Catharenthusroseus showing gasteroprotection with possible involvement of H+, K+ ATPase activity. InInternational Congress of Ethnopharmacology organized by “School of Natural Product Studies” Jadavpur University, Kolkata, India. 12th – 15th February 2012.
  4. Kushal Kesarwani,Vaibhav Mishra, Vijai Lakshmi, Haushila Prasad Pandey and Gautam Palit. Anti-ulcer activity of Dysoxylembinectaiferum: possible involvement of H+, K+ ATPase activity.In Indian Pharma Convention, managed by Udoyag Development Foundation (UDF). Venue at, Delhi Institute of Pharmaceutical Sciences and Reseacrch (DIPSAR), New Delhi, India. 12th February 2012.
  5. VaibhavMishra,VijaiLakshmi, Haushila Prasad Pandeyand GautamPalit. Anti-ulcer activity of andrographaloide: possible involvement of H+, K+ ATPase activity. In 3rd World Congress on Biotechnology by OMICS group, venue at Hyderbad International Convention Centre (HICC), Cyberabad, Hyderabad-500081, Andhra Pradesh, India. 13th – 15th September 2012.
  6. Vinay Kumar Singh,VaibhavMishra, Haushila Prasad Pandey,TadigoppulaNarender and GautamPalit. Peganine Hydrochloride isolated from Peganumharmala seeds Protects gastric mucosa in rats.In19thISCB International Conference (ISCBC-2013). On “Recent Advances and Current Trends in Chemical and BiologicalSciences” Venue at MohanlalSukhadia University Udaipur, Rajasthan, India. 2-5th March, 2013.
  7. AkankshaSrivastava, VaibhavMishraand C.K.M. Tripathi. Production of Cholesterol oxidase enzyme in 3L Bioreactor. In Bangalore India Bio, India’s Biggest Biotech Show. On “Walkway of Discovery”Venue at Hotel Lalit Ashok, Bangalore, India. 10-12thFeb, 2014.
  8. AkankshaSrivastava, VaibhavMishraand C.K.M. Tripathi. Isolation, Production and purification of Cholesterol oxidase enzyme from a novel Streptomyces sp. In 6th NIPER (RBL) CSIR-CDRI symposium. On “Current scenario In Drug Discovery and Development”Venue at CSIR-CDRI, India. 20-22thFeb, 2014.

Workshop/ Symposium attended:

  • Attended Pre-conference workshop on “Publishing Your Research Works in Impact Journals” In 12th International Congress of Ethnopharmacology organized by School of Natural Product Studies Jadavpur University, Kolkata, India. 12thFebruary, 2012.
  • Attended National Symposium on Human Welfare: The Commercial & Ethical Considerations held at Department of Psychology, STPG College, Kadipur.13th-14th December, 2010.
  • Attended a Seminar based on biological techniques organized by IITR,Lucknow, India.2010, (2 Times).
  • Attended a Mini Symposium oncardiovascular disease organized by CDRI, Lucknow, India. 1st week of March, 2010.
  • Attended a conference based on Improvement of science by using new technology organized by IITR, Lucknow, India. 2007.

CHAPTER /REVIEW ARTICLES IN BOOKS :

  • Mishra V and Palit G. Helicobactor pylori and peptic ulcer: cause that transform disease risk. In: Recent Advances in Microbiology. Inc Nova Science Publishers New York, USA, Vol I, 2012.
  • Mishra V, Srivastava A,Pandey HP and Palit G.Microorganisms cause infection in living beings brain regions. In: Recent Advances in Microbiology. Eds. S P Tiwari and Rahul Singh Inc Nova Science Publishers New York, USA,Vol-III (In press).
  • Pandey HPandMishra V. Natural products in ulcers. Book Published in Lambert Academy Publisher Germany 2015 (978-3-659-75999-4).

Technical Expertise:

Animal Handling: Worked with rat, mice and guinea pigs; Dosing routes: oral, i.p, s.c. & im; Collection of blood by cardiac puncture, blood plasma and serum separation, perfusion methods.

Development of animal models: Induction of various models of gastric (cold restraint, alcohol, aspirin and pyloric ligation) and duodenal (Histamine induced duodenal) ulcer in rats and guineapigs; Acetic acid induced chronic gastric ulcer model in rats;

Experimental Reflux Esophagitis in rats;

Depression and Anxiety model in swiss mice;

Ketamine induced Psychosis model in C576/ J mice;

Stress induced acute and chronic model in SD rats.

MACO modelsin SD rats

Surgical procedures: General surgical procedures of dissection of the major biological systems in mammalian and non-mammalian species.

Tissue processing: Membranous fraction, cytosolic fraction and nuclear fraction preparation. Homogenization & sonication procedure for tissue disruption and sample preparation, gastric microsome preparation, Brain parts isolation.

Molecular Biology Techniques:

  • RNA isolation.
  • cDNA synthesis.
  • Polymerase chain reaction (RT-PCR).
  • Agarose gel electrophoresis and software based densitometric analysis of PCR products
  • Western Blotting.

Immunological Techniques:

  • Enzyme linked immunosorbent assay (ELISA).

Biochemical analysis:

  • Spectrophotometricquantitation of RNA and protein.
  • Isolation of gastric microsomes and estimation of inorganic phosphate from gastric microsomes.
  • Isolation of gastric mucosal cells and Intracellular calcium monitoring (Fura-2 labeling) flourimetrically.
  • Enzymatic assays:MPO activity, COX-1 & 2 activity assay, H+K+ATPase activity, Colorimetric assay: MDA, GSH, PGE2.
  • Invitro antioxidants assays.
  • Level of Corticosterone in brain samples by HPLC (UV Detector).

Cell & Tissue Culture:Primary cell isolation using enzymatic digestion method, cell culture preparation & maintenance.

Cell based Assay: MTT assay, Microscopic counting, Trypan blue assay, Ca2+ level and cAMP measurement.

Histological techniques: Formalin fixation, block preparation, sectioning using microtome, paraffin embedding, HE staining & mounting. Know to capture image in bright field microscope.

Other skills:

  • Analytical skills: Statistical analysis using Graph pad Prism software.
  • Computer skills:Microsoft Office (Word, Excel, PowerPoint) and Adobe Photoshop, Endnote library.
  • Proficiency in internet searching and downloading.

Personal details

  • Date of Birth: 05thOct1982
  • Father’s Name: Mr. R. V. Mishra
  • Gender: Male
  • Marital Status: Married
  • Nationality: Indian
  • Permanent address : C/O Mr. R. V. Mishra

House No-1078, Bankers Colony, Parmantpur

Jaunpur, Uttarpradesh, India.

Referees:

Dr. GautamPalit

M.D., M.A.M.S., F.C.C.P., F.C.A.I., F.I.P.S.

Ex- Senior Deputy Director & Head,

Division of Pharmacology

CSIR-Central Drug Research Institute

Lucknow-226031

E-mail:

Mob: +91-9838944441

Dr. Haushila Prasad Pandey

Professor

Department of Biochemistry,

Banaras Hindu University,

Varanasi-221005

Email:

Mob: +91-9453305493

Dr. Manoj Kumar Barthwal

Scientist “C”

Division of Pharmacology

CSIR-Central Drug Research Institute

Lucknow 226031

Email:

Mob: +91-9838541867