Supplementary Figure legend

Fig. S1. The lack of HYL1 suppresses inhibition of bacterial growth mediated by RPM1. Growth of Pst DC3000 (avrRpm1) on plants with differing expression levels for HYL1. Asterisks indicate a significant difference from wild-type plants using a t-test (** = p < 0.01). Similar results were obtained in two independent experiments.

Table S1. Genes > 3.0 fold up- regulated in hyl1 plants

Locus / Gene Description / Fold a
hyl1/WT
At5g44420 / plant defensin protein, putative (PDF1.2a) / 17.7
At2g26020 / plant defensin-fusion protein, putative (PDF1.2b) / 14.7
At1g54020 / myrosinase-associated protein, putative / 14.2
At2g26560 / patatin, putative / 11.3
At1g64160 / disease resistance-responsive family protein / dirigent family protein / 11.0
At1g52040 / myrosinase-binding protein, putative (F-ATMBP) / 10.5
At4g37990 / mannitol dehydrogenase, putative (ELI3-2) / 10.3
At3g55970 / oxidoreductase, 2OG-Fe(II) oxygenase family protein / 9.6
At3g23550 / MATE efflux family protein / 9.3
At5g39110 / germin-like protein, putative / 8.9
At1g26380 / FAD-binding domain-containing protein / 8.7
At1g80320 / oxidoreductase, 2OG-Fe(II) oxygenase family protein / 8.4
At3g49620 / 2-oxoacid-dependent oxidase, putative (DIN11) / 8.1
At2g29460 / glutathione S-transferase, putative / 8.1
At2g39330 / jacalin lectin family protein / 7.5
At5g38930 / germin-like protein, putative / 7.1
At4g29770 / expressed protein / 7.0
At3g21520 / expressed protein / 6.8
At1g63150 / pentatricopeptide (PPR) repeat-containing protein / 6.6
At1g26420 / FAD-binding domain-containing protein / 6.6
At3g21500 / 1-deoxy-D-xylulose 5-phosphate synthase, putative / 1-deoxyxylulose-5-phosphate synthase, putative / DXP-synthase, putative / 6.5
At2g39030 / GCN5-related N-acetyltransferase (GNAT) family protein / 6.5
At1g72260 / thionin (THI2.1) / 6.4
At3g26830 / cytochrome P450 71B15, putative (CYP71B15) / 6.4
At1g26390 / FAD-binding domain-containing protein / 6.2
At4g33720 / pathogenesis-related protein, putative / 6.1
At1g67980 / caffeoyl-CoA 3-O-methyltransferase, putative / 6.0
At1g31280 / PAZ domain-containing protein / piwi domain-containing protein / 5.8
At5g52390 / photoassimilate-responsive protein, putative / 5.8
At5g24420 / glucosamine/galactosamine-6-phosphate isomerase-related / 5.6
At2g29350 / tropinone reductase, putative / tropine dehydrogenase, putative / 5.6
At2g43590 / chitinase, putative / 5.5
At1g52820 / 2-oxoglutarate-dependent dioxygenase, putative / 5.4
At1g44350 / IAA-amino acid hydrolase 6, putative (ILL6) / IAA-Ala hydrolase, putative / 5.3
At1g72830 / CCAAT-binding transcription factor (CBF-B/NF-YA) family protein / 5.2
At1g62590 / pentatricopeptide (PPR) repeat-containing protein / 5.2
At1g52800 / oxidoreductase, 2OG-Fe(II) oxygenase family protein / 5.1
At1g30135 / expressed protein / 5.1
At2g43570 / chitinase, putative / 5.0
At3g57230 / MADS-box protein (AGL16) / 4.8
At3g47340 / asparagine synthetase 1 (glutamine-hydrolyzing) / glutamine-dependent asparagine synthetase 1 (ASN1) / 4.8
At2g47770 / benzodiazepine receptor-related / 4.8
At3g21720 / isocitrate lyase, putative / 4.8
At1g52410 / caldesmon-related / 4.8
At5g06900 / cytochrome P450 family protein / 4.7
At2g46530 / transcriptional factor B3 family protein / auxin-responsive factor AUX/IAA-related / 4.7
At1g51820 / leucine-rich repeat protein kinase, putative / 4.7
At1g02060 / pentatricopeptide (PPR) repeat-containing protein / 4.7
At3g18250 / expressed protein / 4.7

Table S2. Genes > 3.0 fold down- regulated in hyl1 plants

Locus / Gene Description / Fold a
hyl1/WT
At1g01190 / cytochrome P450, putative / 0.10
At2g16005 / MD-2-related lipid recognition domain-containing protein / ML domain-containing protein / 0.14
At3g01260 / aldose 1-epimerase family protein / 0.15
At1g15550 / gibberellin 3-beta-dioxygenase / gibberellin 3 beta-hydroxylase (GA4) / 0.16
At1g27140 / glutathione S-transferase, putative / 0.17
At1g54970 / proline-rich family protein / 0.18
At2g33790 / pollen Ole e 1 allergen and extensin family protein / 0.19
At2g04460 / expressed protein / 0.19
At1g53680 / glutathione S-transferase, putative / 0.20
At5g60660 / major intrinsic family protein / MIP family protein / 0.20
At5g02760 / protein phosphatase 2C family protein / PP2C family protein / 0.21
At4g25250 / invertase/pectin methylesterase inhibitor family protein / 0.21
At1g72240 / expressed protein / 0.21
At4g18510 / Clavata3 / ESR-Related-2 (CLE2) / 0.21
At1g62510 / protease inhibitor/seed storage/lipid transfer protein (LTP) family protein / 0.22
At1g58340 / MATE efflux protein-related / 0.22
At2g47460 / myb family transcription factor (MYB12) / 0.23
At1g74500 / bHLH family protein / 0.23
At5g57530 / xyloglucan:xyloglucosyl transferase, putative / xyloglucan endotransglycosylase, putative / endo-xyloglucan transferase, putative / 0.23
At1g09155 / SKP1 interacting partner 3-related / 0.24
At5g35490 / expressed protein (MRU1) / 0.24
At5g11540 / FAD-binding domain-containing protein / 0.26
At5g35190 / proline-rich extensin-like family protein / 0.26
At3g30160 / hypothetical protein / 0.26
At1g73830 / basic helix-loop-helix (bHLH) family protein / 0.27
At3g05980 / expressed protein / 0.27
At4g39675 / expressed protein / 0.27
At3g49840 / proline-rich family protein / 0.28
At4g40090 / arabinogalactan-protein (AGP3) / 0.28
At5g56870 / beta-galactosidase, putative / lactase, putative / 0.28
At2g14610 / pathogenesis-related protein 1 (PR-1) / 0.29
At1g67110 / cytochrome P450, putative / 0.29
At2g47270 / expressed protein / 0.30
At3g59930 / expressed protein / 0.30
At4g11780 / hypothetical protein / 0.30
At3g25830 / myrcene/ocimene synthase, putative / 0.30
At5g54790 / hypothetical protein / 0.30
At1g02340 / long hypocotyl in far-red 1 (HFR1) / reduced phytochrome signaling (REP1) / basic helix-loop-helix FBI1 protein (FBI1) / reduced sensitivity to far-red light (RSF1) / bHLH protein 26 (BHLH026) (BHLH26) / 0.31
At5g65140 / trehalose-6-phosphate phosphatase, putative / 0.31
At5g08640 / flavonol synthase 1 (FLS1) / 0.31
At1g59940 / two-component responsive regulator / response regulator 3 (ARR3) / 0.31
At1g43330 / myb family protein-related / 0.32
At5g66060 / oxidoreductase, 2OG-Fe(II) oxygenase family protein / 0.32
At2g39040 / peroxidase, putative / 0.32
At3g59340 / expressed protein / 0.32
At3g50560 / short-chain dehydrogenase/reductase (SDR) family protein / 0.32

Table S3. Primers used in this study

Primer / Forward primer / Reverse primer
HYL1-OE / TCGGATCCATGACCTCCACTGATGTTTC / TGGTCGACTTATGCGTGGCTTGCTTCTG
HYL1 / GATCTCTCTGGCAATTGGCA / GGTTGTGAAACACATTGGCT
DRB2 / AAAGTGGTGGCGGAGGAAGC / TGTTTCCTAGGACCGGGACC
DRB3 / GCAGACGACTCTTCCAACGG / TTTCCGGCGAAACTAAATTG
DRB4 / CCAACAGCAACTGCAAGAGA / TTTATTCAGCCCTGCTGTAG
DRB5 / ATATCAGCGACGTACAAGTA / TTCCTGTTCCTGCTTTTGAC
FRK1 / GCGTATAGTGAGTTCAGAATACA / AGTACAAATTCACCAGGTGGTTC
WRKY30 / TCTCAAGGATGTGAAGCCACTA / TGGATAGTGAAGGTTGTGATGC
NHO1 / AGAACTCTCGGGAGGAAGATCC / AGAATGAAAGCACCTGTGCCAT
AtVSP / ACGTCCAGTCTTCGGCATCC / GAGCTTAAAAACCCTTCCAG
Thi2-1 / GTGATCAAACAAGTAAACCAT / AACAAACCTTCTACGACACAT
PDF1-2 / AAGTTTGCTTCCATCATCACC / ATACACACGATTTAGCACCAA
Actin / AGTGTGTCTTGTCTTATCTGGTTCG / AATAGCTGCATTGTCACCCGATACT

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