Principles of Biology Lab Fall, 2006
Week Three: Microscopes and Cells - WITHOUT COMPUTERS
Pre-Lab Quiz:
Procedures: Complete in order; do not skip around or multi-task to try and save time. Note that some sections are to be completed in teams, and others individually.
Complete 3.1 As a Team
Your worksheet will have a diagram to label and questions.
Complete 3.2 In Pairs (no more than 2)
Note separate pair worksheet questions based on lab manual.
Complete 3.4 As a Team
Your "Tour of the Cell" text chapter features many amazing electron microscope images (also called TEM for transmission electron microscope). Look up the following figures and briefly explain featured cell structure(s) AND what each does: Figures 6.8a, 6.11, 6.12, 6.13. 6.14, 6.15, 6.17, 6.18, and 6.24.
Complete 3.5A As a Team
Complete only Trichonympha; note manual Fig. 3.6 and Color Plate 2 to assist you.
No computers available - view/show instructor at 40X; answer new worksheet questions.
Complete 3.5B As a Team
For this exercise, you will be examining 2 different photosynthetic organisms: Anabaena and Spyrogyra. It will be your job to determine which is prokaryotic vs. eukaryotic, and which is unicellular, colonial, or multicellular. For each, you will need to prepare a "wet mount" (see below). No computers available - view/draw at 40X and answer new worksheet questions.
General Wet Mount Procedures Using Liquid Samples
Using a pipette, withdraw SMALL sample - make sure you can see green filaments
Place no more than 1 drop - with filaments - on slide and place a coverslip on the drop
If you used too much liquid, the coverslip will move/float – NOT GOOD
Complete 3.6C - Plant Cells As a Team
No computers available - view/draw at 40X and answer new worksheet questions.
Complete 3.6C - Animal Cells Individually
Each person will prepare his/her own cheek cell - AND complete scientific drawings of them. Each person must complete all parts of this exercise on their own, including magnification. Your instructor may ask to watch you do this as a practical exercise.
Follow procedures in your manual to prepare your stained cheek cell slide preparation.
Using the worksheet provided, hand-draw the entire field of view at 10X objective AND 40X. Good scientific drawings are simple, done in pencil, use stipples and lines (not shading or smudging), and accurately represent the relative size and abundance of things. When complete, label THREE cell structures you can see in your diagram.