Supplementary Table 1 s4

Supplementary Table 1

Random spore analysis data for genes exhibiting synthetic genetic interactions with mad1::NAT. Data is filtered for linkage to MAD1, MFA1, and CAN1

NAME / ORF / Number of Colonies / Colonies / χ2 P ≤ 0.05 /
G418(G) / NAT (N) / G418/ NAT (N) / G : GN 2 : 1 / N : GN 2 : 1 /
AOR1 / YBR231C / 64 / 67 / 0 / 13.037 / 13.648
APQ13 / YJL075C / 300 / 300 / 1 / 60.099 / 60.099
ATP15 / YPL271W / 36 / 58 / 0 / 7.333 / 11.815
BEM1 / YBR200W / 54 / 161 / 4 / 7.432 / 28.900
BIM1 / YER016W / 300 / 300 / 0 / 61.111 / 61.111
BUD30 / YDL151C / 30 / 150 / 0 / 6.111 / 30.556
CBF1 / YJR060W / 267 / 271 / 0 / 54.389 / 55.204
CDC40 / YDR364C / 109 / 122 / 0 / 22.204 / 24.852
CDH1 / YGL003C / 68 / 130 / 3 / 11.029 / 23.550
CHL1 / YPL008W / 41 / 40 / 12 / small / 1.111 / 1.003
CHL4 / YDR254W / 142 / 151 / 0 / 28.926 / 30.759
CIK1 / YMR198W / 257 / 273 / 0 / 52.352 / 55.611
CIN1 / YOR349W / 126 / 137 / 0 / 25.667 / 27.907
CIN2 / YPL241C / 166 / 167 / 0 / 33.815 / 34.019
CSE2 / YNR010W / 70 / 68 / 7 / small / 8.296 / 7.920
CTF18 / YMR078C / 138 / 160 / 0 / 28.111 / 32.593
CTF19 / YPL018W / 75 / 77 / 0 / 15.278 / 15.685
CTF3 / YLR381W / 177 / 154 / 31 / small / 12.952 / 9.320
CTF4 / YPR135W / 66 / 64 / 0 / 13.444 / 13.037
CTF8 / YHR191C / 102 / 127 / 0 / 20.778 / 25.870
ERG2 / YMR202W / 67 / 97 / 4 / small / 9.987 / 15.976
ERG3 / YLR056W / 49 / 44 / 0 / 9.981 / 8.963
GIM3 / YNL153C / 79 / 109 / 0 / 16.093 / 22.204
GIM4 / YEL003W / 54 / 67 / 0 / 11.000 / 13.648
HCM1 / YCR065W / 50 / 55 / 0 / 10.185 / 11.204
KAR3 / YPR141C / 47 / 134 / 0 / 9.574 / 27.296
KEM1 / YGL173C / 51 / 64 / 0 / 10.389 / 13.037
MCM21 / YDR318W / 80 / 127 / 0 / 16.296 / 25.870
MCM22 / YJR135C / 26 / 20 / 0 / 5.296 / 4.074
MDM20 / YOL076W / 48 / 54 / 0 / 9.778 / 11.000
NUP120 / YKL057C / 80 / 107 / 1 / 15.300 / 20.795
NUP133 / YKR082W / 21 / 19 / 0 / 4.278 / 3.870
PAC10 / YGR078C / 400 / 350 / 0 / 81.481 / 71.296
PFD1 / YJL179W / 40 / 54 / 0 / 8.148 / 11.000
PHO80 / YOL001W / 92 / 67 / 1 / 17.742 / 12.657
PHO85 / YPL031C / 300 / 300 / 0 / 61.111 / 61.111
PPA2 / YMR267W / 71 / 186 / 3 / small / 11.630 / 34.921
RIM1 / YCR028C-A / 22 / 22 / 0 / 4.481 / 4.481
RML2 / YEL050C / 30 / 260 / 0 / 6.111 / 52.963
RSM24 / YDR175C / 700 / 820 / 0 / 142.593 / 167.037
RSN1 / YMR266W / 29 / 41 / 0 / 5.907 / 8.352
SIC1 / YLR079W / 115 / 274 / 5 / 18.715 / 50.886
SIT4 / YDL047W / 42 / 200 / 1 / 7.580 / 39.731
SNF4 / YGL115W / 58 / 84 / 4 / 8.214 / 13.370
SNF7 / YLR025W / 49 / 80 / 0 / 9.981 / 16.296
SPT4 / YGR063C / 54 / 48 / 7 / 5.343 / 4.281
SPT10 / YJL127C / 100 / 350 / 0 / 20.370 / 71.296
SSE1 / YPL106C / 64 / 74 / 1 / 12.047 / 14.080
SSN3 / YPL042C / 46 / 35 / 0 / 9.370 / 7.130
TRF4 / YOL115W / 48 / 55 / 0 / 9.778 / 11.204
VPS64 / YDR200C / 19 / 33 / 0 / 3.870 / 6.722
YBP2 / YGL060W / 43 / 38 / 0 / 8.759 / 7.741
YDR433W / YDR433W / 120 / 210 / 0 / 24.444 / 42.778
YKE2 / YLR200W / 300 / 300 / 73 / small / 12.952 / 12.952
YKL053W / YKL053W / 96 / 88 / 0 / 19.556 / 17.926
YLR287C / YLR287C / 156 / 200 / 17 / small / 17.526 / 25.868
YLR312C / YLR312C / 313 / 522 / 34 / 35.237 / 75.515
YNL080C / YNL080C / 18 / 25 / 6 / small / 0.306 / 1.111
YPL017C / YPL017C / 450 / 350 / 0 / 91.667 / 71.296
YPL205C / YPL205C / 54 / 88 / 4 / small / 7.432 / 14.171

Supplementary Table 2

Random spore analysis data for genes exhibiting synthetic genetic interaction with mad2::NAT. Data is filtered for linkage to MAD2, MFA1, and CAN1

Gene / ORF / Number of Colonies / Colony size / χ2 P ≤ 0.05 /
G418 (G) / NAT (N) / G418/ NAT (GN) / G:GN 2:1 / N:GN 2:1 /
APL1 / YJR005W / 83 / 83 / 8 / 10.049 / 10.049
APQ13 / YJL075C / 34 / 47 / 0 / 6.926 / 9.574
ARC18 / YLR370C / 174 / 198 / 51 / small / 4.693 / 7.539
ASE1 / YOR058C / 37 / 51 / 1 / 6.567 / 9.406
BEM1 / YBR200W / 188 / 156 / 1 / 37.287 / 30.771
BEM2 / YER155C / 61 / 56 / 0 / 12.426 / 11.407
BIM1 / YER016W / 51 / 80 / 0 / 10.389 / 16.296
BNA3 / YJL060W / 162 / 145 / 35 / 8.752 / 6.366
BNI1 / YNL271C / 69 / 81 / 0 / 14.056 / 16.500
CBF1 / YJR060W / 77 / 64 / 2 / 13.741 / 11.111
CDH1 / YGL003C / 96 / 96 / 26 / small / 3.233 / 3.233
CHL1 / YPL008W / 29 / 28 / 7 / small / 1.273 / 1.141
CHL4 / YDR254W / 104 / 124 / 0 / 21.185 / 25.259
CIK1 / YMR198W / 88 / 65 / 0 / 17.926 / 13.241
CIN1 / YOR349W / 76 / 62 / 0 / 15.481 / 12.630
CIN2 / YPL241C / 132 / 146 / 0 / 26.889 / 29.741
CLB2 / YPR119W / 87 / 99 / 0 / 17.722 / 20.167
CSE2 / YNR010W / 25 / 13 / 4 / small / 2.030 / 0.300
CTF18 / YNR078C / 40 / 59 / 0 / 8.148 / 12.019
CTF19 / YPL018W / 112 / 122 / 0 / 22.815 / 24.852
CTF3 / YLR381W / 139 / 141 / 47 / small / 2.218 / 2.394
CTF4 / YPR135W / 80 / 99 / 0 / 16.296 / 20.167
CTF8 / YHR191C / 180 / 259 / 46 / 6.980 / 18.627
CTK2 / YJL006C / 150 / 158 / 0 / 30.56 / 32.19
DIA2 / YOR080W / 61 / 74 / 29 / small / 0.020 / 0.506
DOA4 / YDR069C / 26 / 28 / 0 / 5.296 / 5.704
ERG2 / YMR202W / 107 / 122 / 49 / small / 0.106 / 0.686
GCS1 / YDL226C / 101 / 79 / 34 / small / 1.643 / 0.218
GIM3 / YNL153C / 61 / 77 / 0 / 12.426 / 15.685
GIM4 / YEL003W / 65 / 274 / 0 / 13.241 / 55.815
HCM1 / YCR065W / 164 / 230 / 10 / 24.276 / 37.431
HPC2 / YBR215W / 74 / 65 / 0 / 15.074 / 13.241
HSP42 / YDR171W / 76 / 65 / 7 / 9.434 / 7.359
HTZ1 / YOL012C / 83 / 103 / 7 / 10.776 / 14.669
ISA1 / YLL027W / 56 / 101 / 0 / 11.407 / 20.574
KAR3 / YPR141C / 111 / 120 / 0 / 22.611 / 24.444
KCS1 / YDR017C / 27 / 32 / 0 / 5.500 / 6.519
MCM16 / YPR046W / 78 / 93 / 0 / 15.889 / 18.944
MCM19 / YBR107C / 61 / 77 / 5 / small / 8.028 / 11.152
MCM21 / YDR318W / 138 / 144 / 0 / 28.111 / 29.333
MCM22 / YJR135C / 71 / 88 / 0 / 14.463 / 17.926
MDM20 / YOL076W / 56 / 61 / 0 / 11.407 / 12.426
MSR1 / YHR091C / 45 / 29 / 1 / 8.188 / 4.950
NAT3 / YPR131C / 41 / 44 / 25 / small / 0.250 / 0.106
PAC10 / YGR078C / 70 / 102 / 27 / small / 0.538 / 3.638
PFD1 / YJL179W / 30 / 23 / 0 / 6.111 / 4.685
PLC1 / YPL268W / 121 / 103 / 22 / small / 8.446 / 5.673
RAD10 / YML095C / 49 / 51 / 9 / small / 3.375 / 3.697
RAD23 / YEL037C / 76 / 59 / 0 / 15.481 / 12.019
RSM24 / YDR175C / 37 / 31 / 0 / 7.537 / 6.315
RSN1 / YMR266W / 155 / 193 / 90 / small / 0.520 / 0.122
SAG1 / YJR004C / 120 / 95 / 11 / 14.934 / 10.241
SHM2 / YLR058C / 64 / 56 / 19 / small / 1.659 / 0.880
SIC1 / YLR079W / 104 / 130 / 0 / 21.185 / 26.481
SIN4 / YNL236W / 60 / 40 / 18 / small / 1.504 / 0.056
SLI15 / YBR156C / 121 / 99 / 8 / 17.410 / 13.115
SLK19 / YOR195W / 31 / 24 / 1 / 5.354 / 3.944
SSE1 / YPL106C / 78 / 82 / 0 / 15.889 / 16.704
TDH2 / YJR009C / 232 / 224 / 34 / small / 20.597 / 19.214
WHI5 / YOR083W / 163 / 157 / 0 / 33.204 / 31.981
YAF9 / YNL107W / 95 / 89 / 21 / 4.933 / 4.091
YBP2 / YGL060W / 206 / 190 / 1 / small / 40.953 / 37.695
YDR433W / YDR433W / 130 / 150 / 0 / 26.481 / 30.556
YJL058C / YJL058C / 43 / 35 / 1 / 7.782 / 6.162
YKE2 / YLR200W / 46 / 95 / 0 / 9.370 / 19.352
YKL053W / YKL053W / 96 / 107 / 10 / 11.100 / 13.178
YLR287C / YLR287C / 75 / 106 / 0 / 15.278 / 21.593
YLR312C / YLR312C / 20 / 20 / 0 / 4.074 / 4.074
YMR102C / YMR102C / 35 / 33 / 6 / small / 2.628 / 2.303

Supplementary Table 3

Random spore analysis data for genes exhibiting synthetic genetic interaction with bub3::NAT. Data is filtered for linkage to BUB3, MFA1, and CAN1

Gene / ORF / Number of Colonies / Colony size / χ2 P ≤ 0.05 /
G418 (G) / NAT (N) / G418/ NAT (GN) / G:GN 2:1 / N:GN 2:1 /
ALG6 / YOR002W / 184 / 126 / 9 / 29.084 / 17.600
AOR1 / YBR231C / 58 / 73 / 4 / 8.214 / 11.177
ARP1 / YHR129C / 45 / 59 / 5 / 4.991 / 7.642
ARP8 / YOR141C / 51 / 41 / 4 / 6.848 / 4.930
ASE1 / YOR058C / 76 / 62 / 11 / 6.828 / 4.465
ATP15 / YPL271W / 72 / 105 / 0 / 14.667 / 21.389
BEM1 / YBR200W / 90 / 256 / 11 / 9.326 / 41.775
BIK1 / YCL029C / 40 / 27 / 0 / 8.148 / 5.500
BIM1 / YER016W / 93 / 153 / 2 / 16.985 / 29.177
BNI1 / YNL271C / 104 / 102 / 5 / 16.513 / 16.114
BUD30 / YDL151C / 60 / 72 / 0 / 12.222 / 14.667
CBF1 / YJR060W / 42 / 29 / 0 / 8.556 / 5.907
CDH1 / YGL003C / 67 / 77 / 0 / 13.648 / 15.685
CHL4 / YDR254W / 79 / 54 / 1 / 15.097 / 10.015
CIK1 / YMR198W / 65 / 73 / 0 / 13.241 / 14.870
CIN1 / YOR349W / 97 / 84 / 0 / 19.759 / 17.111
CIN2 / YPL241C / 76 / 77 / 4 / 11.774 / 11.973
CLB1 / YGR108W / 200 / 200 / 21 / 23.010 / 23.010
CLB3 / YDL155W / 118 / 88 / 0 / 24.037 / 17.926
CLB5 / YPR120C / 24 / 29 / 0 / 4.889 / 5.907
CRN1 / YLR429W / 140 / 128 / 16 / 15.231 / 13.037
CSM3 / YMR048W / 61 / 55 / 4 / 8.803 / 7.627
CTF18 / YMR078C / 41 / 42 / 2 / 6.485 / 6.685
CTF19 / YPL018W / 52 / 40 / 3 / 7.837 / 5.476
CTF3 / YLR381W / 80 / 87 / 0 / 16.296 / 17.722
CTF4 / YPR135W / 209 / 107 / 9 / 34.089 / 13.910
CTF8 / YHR191C / 81 / 72 / 11 / 7.708 / 6.136
DIA2 / YOR080W / 70 / 79 / 0 / 14.259 / 16.093
DOA4 / YDR069C / 89 / 124 / 0 / 18.130 / 25.259
GCS1 / YDL226C / 36 / 46 / 1 / 6.364 / 8.391
GIM3 / YNL153C / 78 / 86 / 5 / 11.349 / 12.930
GIM4 / YEL003W / 107 / 108 / 0 / 21.796 / 22.000
HIR1 / YBL008W / 69 / 64 / 6 / 8.824 / 7.869
ISA1 / YLL027W / 59 / 160 / 6 / 6.923 / 26.879
KAR3 / YPR141C / 33 / 88 / 0 / 6.722 / 17.926
KCS1 / YDR017C / 58 / 63 / 3 / 9.030 / 10.028
KEM1 / YGL173C / 104 / 99 / 22 / 5.820 / 5.093
KIP3 / YGL216W / 87 / 88 / 0 / 17.722 / 17.926
LGE1 / YPL055C / 67 / 134 / 13 / 4.280 / 16.163
LSM1 / YJL124C / 151 / 153 / 11 / 20.925 / 21.316
LSM6 / YDR378C / 66 / 86 / 3 / 10.628 / 14.648
MCM16 / YPR046W / 137 / 123 / 0 / 27.907 / 25.056
MCM21 / YDR318W / 67 / 69 / 3 / 10.828 / 11.229
MCM22 / YJR135C / 177 / 138 / 9 / 27.687 / 19.955
MDM12 / YOL009C / 54 / 57 / 7 / 5.343 / 5.885
MRC1 / YCL060C / 88 / 91 / 7 / 11.742 / 12.324
MRPL17 / YNL252C / 108 / 114 / 15 / small / 10.076 / 11.142
NUP84 / YDL116W / 34 / 22 / 0 / 6.926 / 4.481
PAC2 / YER007W / 43 / 57 / 5 / 4.622 / 7.258
PAC10 / YGR078C / 80 / 88 / 0 / 16.296 / 17.926
PFD1 / YJL179W / 124 / 83 / 0 / 25.259 / 16.907
PHO80 / YOL001W / 200 / 200 / 11 / 30.588 / 30.588
PHO85 / YPL031C / 47 / 41 / 0 / 9.574 / 8.352
PPA2 / YMR267W / 105 / 123 / 0 / 21.389 / 25.056
PPZ1 / YML016C / 200 / 200 / 9 / 32.285 / 32.285
RIM21 / YNL294C / 143 / 96 / 11 / 19.366 / 10.425
RML2 / YEL050C / 200 / 200 / 3 / 37.766 / 37.766
RRP6 / YOR001W / 200 / 200 / 18 / 25.132 / 25.132
RTF1 / YGL244W / 113 / 126 / 28 / 4.694 / 6.481
SEM1 / YDR363W-A / 152 / 129 / 5 / 26.162 / 21.527
SIC1 / YLR079W / 62 / 46 / 0 / 12.630 / 9.370
SIN4 / YNL236W / 65 / 91 / 2 / 11.313 / 16.579
SIT4 / YDL047W / 52 / 204 / 1 / 9.609 / 40.546
SNF4 / YGL115W / 159 / 116 / 8 / 24.943 / 16.428
SNF7 / YLR025W / 32 / 67 / 3 / 3.934 / 10.828
SPO21 / YOL091W / 124 / 155 / 17 / 11.702 / 17.340
SPT10 / YJL127C / 59 / 81 / 0 / 12.019 / 16.500
SPT21 / YMR179W / 200 / 200 / 56 / 6.162 / 6.162
SSN3 / YPL042C / 21 / 33 / 0 / 4.278 / 6.722
TOF1 / YNL273W / 147 / 142 / 7 / 23.398 / 22.399
TOP1 / YOL006C / 176 / 168 / 26 / 15.506 / 14.129
TRF4 / YOL115W / 54 / 74 / 1 / 10.015 / 14.080
VIK1 / YPL253C / 97 / 81 / 0 / 19.759 / 16.500
VIP1 / YLR410W / 110 / 78 / 12 / 12.349 / 6.600
VPS64 / YDR200C / 183 / 176 / 2 / 35.280 / 33.856
YAF9 / YNL107W / 32 / 32 / 3 / 3.934 / 3.934
YDJ1 / YNL064C / 37 / 42 / 0 / 7.537 / 8.556
YDR433W / YDR433W / 26 / 42 / 1 / 4.346 / 7.580
YKE2 / YLR200W / 63 / 86 / 0 / 12.833 / 17.519
YLR287C / YLR287C / 85 / 91 / 0 / 17.315 / 18.537
YLR312C / YLR312C / 70 / 67 / 0 / 14.259 / 13.648
YNL080C / YNL080C / 65 / 91 / 0 / 13.241 / 18.537
YNL171C / YNL171C / 59 / 112 / 6 / 6.923 / 17.263
YOL014W / YOL014W / 77 / 65 / 12 / 6.429 / 4.447
YOR008C-A / YOR008C-A / 147 / 153 / 12 / 19.383 / 20.544
YPL017C / YPL017C / 56 / 43 / 5 / 7.066 / 4.622
YPL205C / YPL205C / 41 / 47 / 3 / 5.671 / 6.849

Supplementary Table 4

Random spore analysis data for genes exhibiting synthetic genetic interaction with mad3::NAT. Data is filtered for linkage to MAD3, MFA1 and CAN1

Gene / ORF / Number of Colonies / Colony size / χ2 P ≤ 0.05
G418 (G) / NAT (N) / G418/ NAT (GN) / G:GN 2:1 / N:GN 2:1
APQ13 / YJL075C / 47 / 46 / 0 / 9.574 / 9.370
BIM1 / YER016W / 122 / 154 / 0 / 24.852 / 31.370
CIK1 / YMR198W / 57 / 71 / 0 / 11.611 / 14.463
CSE2 / YNR010W / 63 / 56 / 23 / small / 0.685 / 0.258
CTF18 / YMR078C / 57 / 74 / 49 / small / 3.230 / 0.954
CTF19 / YPL018W / 118 / 110 / 13 / small / 13.161 / 11.686
KAR3 / YPR141C / 77 / 122 / 0 / 15.685 / 24.852
MCK1 / YNL307C / 52 / 57 / 3 / 7.837 / 8.831
RIM13 / YMR154C / 164 / 131 / 0 / 33.407 / 26.685
SAT4 / YCR008W / 102 / 40 / 20 / small / 6.418 / 0.000
SIC1 / YLR079W / 220 / 238 / 0 / 44.815 / 48.481
YDR433W / YDR433W / 110 / 190 / 0 / 22.407 / 38.704

7