Table. 1 The ORFs and their annotation of phage PE3-1 genome from blastp

ORF / Position (bp) / NCBI Accession no. / Predicted function in NCBI database (E-value) / Predicted function in HHpred database (E-value)
01 / 142..300 / AIB06956.1 / Unknown [YP_002003788.1,
Enterobacteria phage EcoDS1, 3E-20]
02 / 1472..1804 / AIB06957.1 / DNA mimic ocr [YP_002003736.1,
Enterobacteria phage EcoDS1, 1E-64] / Gene 0.3 protein [all helical, gene regulation][Enterobacteria phage T7, 1.2E-40]
03 / 1801..1953 / AIB06958.1 / Unknown [YP_009007138.1,
Citrobacter phage CR44b, 3E-24]
04 / 2022..2189 / AIB06959.1 / Unknown [YP_338092.1,
Enterobacteria phage K1F, 6E-26]
05 / 2279..2476 / AIB06960.1 / Unknown [YP_002003739.1,
Enterobacteria phage EcoDS1, 7E-33]
06 / 2476..2868 / AIB06961.1 / Unknown [YP_002003740.1,
Enterobacteria phage EcoDS1, 2E-86]
07 / 2966..5647 / AIB06962.1 / RNA polymerase [YP_338094,
Enterobacteria phage K1F, 0.0] / DNA-directed RNA polymerase [Enterobacteria phage T7, 1.8E-204]
08 / 5660..5875 / AIB06963.1 / Unknown [YP_338095.1,
Enterobacteria phage K1F, 2E-37]
09 / 6044..6223 / AIB06964.1 / Unknown [YP_009005118.1,
Cronobacter phage Dev2, 5E-31]
10 / 6226..6513 / AIB06965.1 / Unknown [YP_009007144.1,
Citrobacter phage CR44b, 3E-35]
11 / 6531..7553 / AIB06966.1 / DNA ligase [YP_338096,
Enterobacteria phage K1F, 0.0] / ATP-dependent DNA ligase [Psychromonas SP, 1.1E-47]
12 / 7711..7944 / AIB06967.1 / Unknown [NP_052076.1,
Yersinia phage phiYeO3-12, 3E-33] / HNH homing endonuclease [Bacillus phage SPO1, 1.1E-16]
13 / 8071..8331 / AIB06968.1 / Unknown [YP_009007146.1,
Citrobacter phage CR44b, 3E-51]
14 / 8444..8764 / AIB07002.1 / Unknown [YP_009005122.1,
Cronobacter phage Dev2, 4E-50]
15 / 8825..9001 / AIB07003.1 / Host RNAP inhibitor [YP_338101,
Enterobacteria phage K1F, 8E-27] / Bacterial RNA polymerase inhibitor [Enterobacteria phage T7, 3.1E-30]
16 / 9059..9754 / AIB06969.1 / Predicted helix-destabilizing protein [YP_009007148.1,
Citrobacter phage CR44b, 5E-159] / Helix-destabilizing protein [Enterobacteria phage T7, 2.4E-47]
17 / 10007..10204 / AIB06970.1 / Putative HNH endonuclease [YP_002308399,
Kluyvera phage Kvp1, 3E-29] / TC3 transposase [Caenorhabditis elegans, 2.8E-05]
18 / 10188..10637 / AIB06971.1 / Endonuclease [YP_009007150,
Citrobacter phage CR44b, 9E-87] / Endonuclease [Enterobacteria phage T7, 4.4E-40]
19 / 10637..11107 / AIB06972.1 / Unknown [YP_009007151.1,
Citrobacter phage CR44b, 5E-85]
20 / 11097..11555 / AIB06973.1 / Lysozyme [YP_338105.1,
Enterobacteria phage K1F, 1E-103] / T7 lysozyme [Enterobacteria phage T7, 3.4E-36]
21 / 11580..11795 / AIB06974.1 / Unknown [YP_338106.1,
Enterobacteria phage K1F, 2E-28]
22 / 11992..13692 / AIB06975.1 / Primase/helicase [YP_338107.1,
Enterobacteria phage K1F, 0.0] / DNA primase/helicase [Enterobacteria phage T7, 1.8E-57]
23 / 13735..13899 / AIB06976.1 / Unknown [YP_009007157.1,
Citrobacter phage CR44b, 9E-30]
24 / 13963..16134 / AIB06977.1 / DNA polymerase [YP_338108.1, Enterobacteria phage K1F, 0.0] / DNA polymerase [Enterobacteria phage T7, 1.5E-97]
25 / 16134..16433 / AIB06978.1 / Unknown [YP_002003761.1,
Enterobacteria phage EcoDS1, 2E-60]
26 / 16430..16639 / AIB06979.1 / Unknown [YP_338112.1,
Enterobacteria phage K1F, 2E-40]
27 / 16636..17502 / AIB06980.1 / Exonuclease [YP_009007164,
Citrobacter phage CR44b, 0.0] / 5'-exonuclease [Enterobacteria phage T5, 1.7E-41]
28 / 17713..17985 / AIB06981.1 / Unknown [YP_009005136.1,
Cronobacter phage Dev2, 1E-28]
29 / 18000..18224 / AIB06982.1 / Unknown [YP_338115.1,
Enterobacteria phage K1F, 1E-42]
30 / 18265..18633 / AIB06983.1 / Unknown [YP_009007168.1,
Citrobacter phage CR44b, 2E-85] / Putative acetyl transferase [Pyrococcus furiosus
, 4.8E-4]
31 / 18626..18874 / AIB06984.1 / Conserved phage virion protein [YP_009007169.1,
Citrobacter phage CR44b, 6E-43]
32 / 18886..20454 / AIB06985.1 / Head to tail connector [YP_338118.1,
Enterobacteria phage K1F, 0.0] / Head-to-tail joining protein [Enterobacteria phage T7, 1.1E-64]
33 / 20560..21444 / AIB06986.1 / Scaffold protein [YP_338119.1,
Enterobacteria phage K1F, 0.0]
34 / 21571..22620 / AIB06987.1 / Predicted minor capside protein [YP_009007173.1,
Citrobacter phage CR44b, 0.0] / Major capsid protein 10A [Enterobacteria phage T7, 1.7E-32]
35 / 22620..22883 / AIB06988.1 / Unknown [YP_009005143.1,
Cronobacter phage Dev2, 4E-29]
36 / 22942..23508 / AIB06989.1 / Tail tube [YP_338121.1,
Enterobacteria phage K1F, 3E-134] / Tail tubular protein A [Enterobacteria phage T7, 1.8E-36]
37 / 23508..23735 / AIB06990.1 / RNA-binding protein [WP_017494337,
Gamma proteobacterium WG36, 3E-22] / RNA binding protein [Pyrococcus horikoshii, 2E-17]
38 / 23745..26102 / AIB06991.1 / Tail tube [YP_338122.1,
Enterobacteria phage K1F, 0.0]
39 / 26179..26649 / AIB06992.1 / Internal virion protein [YP_338123,
Enterobacteria phage K1F, 3E-107] / Ribosomal-protein-S5-alanine N-acetyltransferase [Vibrio fischeri, 4.3E-16]
40 / 26658..27221 / AIB06993.1 / Internal virion protein [YP_338124.1,
Enterobacteria phage K1F, 1E-132]
41 / 27233..29515 / AIB06994.1 / Internal virion protein [YP_338125.1,
Enterobacteria phage K1F, 0.0]
42 / 29520..33407 / AIB06995.1 / Internal virion protein [YP_338126.1,
Enterobacteria phage K1F, 0.0] / Lysozyme-like chitinolytic enzyme [Ralstonia, 1.5E-4]
43 / 33473..35659 / AIB06996.1 / Hypothetical protein [WP_050438815.1, Escherichia coli, 0.0] / Tailspike protein [Escherichia phage CBA120, 2.5E-17]
44 / 35731..35925 / AIB06997.1 / Type II holing [YP_009005151.1,
Cronobacter phage Dev2, 3E-35]
45 / 35922..36185 / AIB06998.1 / predicted DNA packaging protein [YP_009007186.1,
Citrobacter phage CR44b, 3E-53]
46 / 36335..36736 / AIB06999.1 / phage lambda Rz-like lysis protein [YP_338130.1,
Enterobacteria phage K1F, 9E-88]
47 / 36766..38493 / AIB07000.1 / Putative DNA maturation [CAJ29395.1,
Enterobacteria phage K1F, 0.0] / DNA maturase B [Enterobacteria phage T7, 2.2E-46]
48 / 38510..39052 / AIB07001.1 / HNH endonuclease [WP_050486085.1,
Enterobacteriaceae, 3E-43] / HNH homing endonuclease [Bacillus phage SPO1, 3.8E-20]