Darrell Jay Grimes

DARRELL JAY GRIMES

The University of Southern Mississippi2325 Starfish Road

Gulf Coast Research LaboratoryGautier, MS 39553

300 Laurel Oak Drive228-497-2953

Ocean Springs, MS 39564Cell: 228-806-7700

Education:

Pre-med, The University of Iowa, 1962-1964

B.A., Biology, Drake University, 1966

M.A., Biology, Drake University, 1968

Ph.D., Microbiology, Colorado State University, 1971

Professional Recognition:

Fellow, American Academy of Microbiology

Fellow, American Association for the Advancement of Science

Professional Positions:

1/97-Professor, Department of Coastal Sciences (Marine Microbiology), The University of Southern Mississippi, Ocean Springs, MS (tenured 1998)

10/07-10/09Chairman, Board of Directors, The University of Southern Mississippi Research Foundation

7/04-8/07Provost and Vice President for Academic Affairs, The University of Southern Mississippi (Hattiesburg and Gulf Coast positions were combined in 7/04)

7/03-7/04Provost – Gulf Coast Campus and Sites, The University of Southern Mississippi

7/02-6/03Provost – Hattiesburg Campus, The University of Southern Mississippi

2/01-6/02Dean, College of Marine Sciences, The University of Southern Mississippi

10/99-2/01Dean, Institute of Marine Sciences, The University of Southern Mississippi

1/97-10/99Director, Institute of Marine Sciences, The University of Southern Mississippi

1/97-7/07Director, Gulf Coast Research Laboratory, The University of Southern Mississippi, Ocean Springs, MS

8/95-12/95Senior Partner, Interagency Environmental Technologies Office, National Science and Technology Council, Executive Office of the President, Washington, D.C. (on detail from DOE, IETO terminated 12/95 for lack of funding)

1/91-8/93Executive Secretary, Biotechnology Research Subcommittee, Committee on Life Sciences and Health, FCCSET, Washington, D.C.

10/90-1/97Microbiologist (GS-15/10), Environmental Sciences Division (ER-74), Office of Health and Environmental Research, Office of Energy Research, U.S. Department of Energy, Germantown, MD (Founder and Manager, Microbial Genome Program; Founder and Co-Manager, Natural and Accelerated Bioremediation Research Program)

6/87-10/90 Professor, Department of Microbiology, University of New Hampshire, Durham, NH

6/89-7/90 Director, Jackson Estuarine Laboratory, University of New Hampshire

6/87-7/90 Director, Sea Grant College Program, University of New Hampshire

6/87-6/89Director, Institute of Marine Science and Ocean Engineering, University of New Hampshire (IMSOE terminated 4/90)

1/80-6/87Research Associate Professor, Department of Microbiology, University of Maryland, College Park, MD

9/76-9/82Associate Professor, Department of Biology, University of Wisconsin-La Crosse, La Crosse, WI

8/71-8/76Assistant Professor, Department of Biology, UW-LaCrosse (tenured 1975)

6/68-9/68Inspector, Economy Forms Corp., Des Moines, IA

9/66-6/68Teaching Assistantship (Biology, Field Botany, Microbiology, Nurse's Anatomy and Physiology, Nurse's Microbiology), Drake University, Des Moines, IA

Adjunct and Visiting Positions:

6/09Visiting Professor, National University of Singapore, Singapore

3/09-6/10Adjunct Professor, Department of Environmental Sciences, Louisiana State University, Baton Rouge

7/81-9/83Visiting Associate Professor, Department of Microbiology, University of Maryland

1/81-6/81Research Associate, Department of Microbiology, University of Maryland

Research Interests:

Biology and ecology of Vibrio

Systematics of the generaChromobacterium, Pseudomonas, Vibrio, and Vogesella

Microbiological quality of water resources

Bacteriology of marine fish, especially elasmobranchs

Long-term survival of bacteria in aquatic habitats

Microbiology and bioremediationof ocean and estuarine wastes, especiallypetroleum

Theses:

Grimes, D.J. 1968. Microbial degradation of chlorinated hydrocarbon insecticides. M.A. Thesis. Drake University, Des Moines, IA. (Dr. Jerrell F. Fair, Advisor)

Grimes, D.J. 1971. Bioconcentration of chlorinated hydrocarbon insecticides by aquatic bacteria. Ph.D. Dissertation, Colorado State University, Fort Collins, CO. (Dr. Sumner M. Morrison, Major Professor, deceased)

Publications (refereed journal articles):

1. Grimes, D.J. 1975. Release of sediment-bound fecal coliforms by dredging. Appl. Microbiol. 29:109-111.

2. Grimes, D.J. and S.M. Morrison. 1975. Bacterial bioconcentration of chlorinated hydrocarbon insecticides from aqueous systems. Microb. Ecol. 2:43-59.

3. Grimes, D.J. 1980. Bacteriological water quality effects of hydraulically dredging contaminated Upper Mississippi River bottom sediment. Appl. Environ. Microbiol. 39:782-789.

4. Grimes, D.J. 1982. Bacteriological water quality effects of clamshell dredging. J. Freshwater Ecol. 1:407-419.

5. LaLiberte, P. and D.J. Grimes. 1982. Survival of Escherichia coli in lake bottom sediment. Appl. Environ. Microbiol. 43:623-628.

6. Mach, P.A. and D.J. Grimes. 1982. R-plasmid transfer in a wastewater treatment plant. Appl. Environ. Microbiol. 44:1395-1403.

7. Xu, H.-S., N. Roberts, F.L. Singleton, R.W. Attwell, D.J. Grimes and R.R. Colwell. 1982. Survival and viability of nonculturable Escherichia coli and Vibrio cholerae in the estuarine and marine environment. Microb. Ecol. 8:313-323.

8. Grimes, D.J. and R.R. Colwell. 1983. Survival of pathogenic organisms in the Anacostia and Potomac Rivers and the Chesapeake Bay Estuary. J. Wash. Acad. Sci. 73:45-50.

9. Pellett, S., D.V. Bigley and D.J. Grimes. 1983. Distribution of Pseudomonas aeruginosa in a riverine ecosystem. Appl. Environ. Microbiol. 45:328-332.

10. Grimes, D.J., F.L. Singleton and R.R. Colwell. 1984. Allogenic succession of marine bacterial communities in response to pharmaceutical waste. J. Appl. Bacteriol. 57:247-261.

11. Grimes, D.J., F.L. Singleton, J. Stemmler, L.M. Palmer, P. Brayton and R.R. Colwell. 1984. Microbiological effects of wastewater effluent discharge into coastal waters of Puerto Rico. Water Res. 18:613-619.

12. Grimes, D.J., J. Stemmler, H. Hada, E.B. May. D. Maneval, F.M. Hetrick, R.T. Jones, M. Stoskopf and R.R. Colwell. 1984. Vibrio species associated with mortality of sharks held in captivity. Microb. Ecol. 10:271-282.

13. Roszak, D.B., D.J. Grimes and R.R. Colwell. 1984. Viable but non-recoverable stage of Salmonella enteritidis in aquatic systems. Can. J. Microbiol. 30:334-338.

14. Palmer, L.M., A.M. Baya, D.J. Grimes and R.R. Colwell. 1984. Molecular genetic and phenotypic alteration of Escherichia coli in natural water microcosms containing toxic chemicals. FEMS Microbiol. Lett. 21:169-173.

15. Hill, G.A. and D.J. Grimes. 1984. Seasonal study of a freshwater lake and migratory waterfowl for Campylobacter jejuni. Can J. Microbiol. 30:845-849.

16. West, P.A., G.C. Okpokwasili, P.R. Brayton, D.J. Grimes and R.R. Colwell. 1984. Numerical taxonomy of phenanthrene-degrading bacteria isolated from the Chesapeake Bay. Appl. Environ. Microbiol. 48:988-993.

17. Walker, P.J. and D.J. Grimes. 1984. A note on Yersinia enterocolitica in a swine farm watershed. J. Appl. Bacteriol. 58:139-143.

18. Grimes, D.J., R.R. Colwell, J. Stemmler, H. Hada, D. Maneval, F.M. Hetrick, E.B. May, R.T. Jones and M. Stoskopf. 1984. Vibrio species as agents of elasmobranch disease. Helgolander Meeresunters. 37:309-315.

19. Colwell, R.R. and D.J. Grimes. 1984. Vibrio diseases of marine fish populations. Helgolander Meeresunters. 37:265-287.

20. Grimes, D.J., S.H. Gruber and E.B. May. 1985. Experimental infection of lemon sharks, Negaprion brevirostris (Poey), with Vibrio species. J. Fish Dis. 8:173-180.

21. Grimes, D.J., P. Brayton, R.R. Colwell and S.H. Gruber. 1985. Vibrios as autochthonous flora of neritic sharks. Syst. Appl. Microbiol. 6:221-226.

22. Colwell, R.R., P.R. Brayton, D.J. Grimes, D.B. Roszak, S.A. Huq and L.M. Palmer. 1985. Viable but non-culturableVibrio cholerae and related pathogens in the environment: Implications for release of genetically engineered microorganisms. Bio/Technology 3:817-820.

23. Baya, A.M., P.R. Brayton, V.L. Brown, D.J. Grimes, E. Russek-Cohen and R.R. Colwell. 1986. Coincident plasmids and antimicrobial resistance in marine bacteria isolated from polluted and unpolluted Atlantic Ocean samples. Appl. Environ. Microbiol. 51:1285-1292.

24. Brayton, P.R., R.B. Bode, R.R. Colwell, M.T. MacDonell, H.L. Hall, D.J. Grimes, P.A. West and T.N. Bryant. 1986. Vibrio cincinnatiensis sp. nov., a new human pathogen. J. Clin. Microbiol. 23:104-108.

25. Okpokwasili, G.C., C.C. Somerville, M. Sullivan, D.J. Grimes and R.R. Colwell. 1986. Plasmid mediated degradation of hydrocarbons in estuarine bacteria. Oil Chem. Pollut. 3:117-129.

26. Grimes, D.J. and R.R. Colwell. 1986. Viability and virulence of Escherichia coli suspended by membrane chamber in semitropical ocean water. FEMS Microbiol. Lett. 34:161-165.

27. Grimes, D.J., P.R. Brayton, P.A. West, F.L. Singleton and R.R. Colwell. 1986. The probabilistic identification of Vibrio spp. isolated from surface seawater with special reference to Vibrio campbellii. Lett. Appl. Microbiol. 2:93-95.

28. Grimes, D.J., R.W. Attwell, P.R. Brayton, L.M. Palmer, D.M. Rollins, D.B. Roszak, F.L. Singleton, M.L. Tamplin and R.R. Colwell. 1986. The fate of enteric pathogenic bacteria in estuarine and marine environments. Microbiological Sciences 3:324-329.

29. Huq, A., S.A. Huq, D.J. Grimes, M. O'Brien, K.H. Chu, J.M. Capuzzo and R.R. Colwell. 1986. Colonization of the gut of the blue crab (Callinectes sapidus) by Vibrio cholerae. Appl. Environ. Microbiol. 52:586-588.

30. Attaway, H., J.O. Nelson, A.M. Baya, M.J. Voll, W.E. White, D.J. Grimes and R.R. Colwell. 1987. Bacterial detoxification of diisopropyl fluorophosphate. Appl. Environ. Microbiol. 53:1685-1689.

31. Chapelle, F.H., J.L. Zelibor, Jr., D.J. Grimes and L.L. Knobel. 1987. Bacteria in deep coastal plain sediments of Maryland: a possible source of CO2 to groundwater. Water Resources Res. 23:1625-1632.

32. Zelibor, J.L., Jr., M.W. Doughten, D.J. Grimes and R.R. Colwell. 1987. Testing for bacterial resistance to arsenic in monitoring well water by the direct viable counting method. Appl. Environ. Microbiol. 53:2929-2934.

33. Toranzo, A.E., A.M. Baya, B.S. Roberson, J.L. Barja, D.J. Grimes and F.M. Hetrick. 1987. Specificity of slide agglutination test for detecting bacterial fish pathogens. Aquaculture 61:81-97.

34. Knight, I.T., D.J. Grimes and R.R. Colwell. 1988. Bacterial hydrolysis of urea in the tissues of carcharhinid sharks. Can. J. Fish. Aquatic Sci. 45:357-360.

35. Youngren-Grimes, B.L., D.J. Grimes and R.R. Colwell. 1988. Growth of Vibrio cholerae, Vibrio parahaemolyticus, and Vibrio vulnificus under strict anaerobic conditions. Syst. Appl. Microbiol. 11:13-15.

36. Grimes, D.J., J. Burgess, J.A. Crunkleton, Jr., P.R. Brayton and R.R. Colwell. 1989. Potential invasive factors associated with Vibrio carchariae, an opportunistic pathogen for sharks. J. Fish Dis. 12:69-72.

37. Grimes, D.J. 1990. Procaryotes and chondrichthyans: an ancient relationship with public health implications for an expanding fishery. Chondros 2(3):1-2.

38. O'Neill, K.R., S.H. Jones and D.J. Grimes. 1990. Incidence of Vibrio vulnificus in northern New England water and shellfish. FEMS Microbiol. Lett. 72:163-168.

39. Grimes, D.J. 1991. Ecology of estuarine bacteria capable of causing human disease: a review. Estuaries 14:345-360.

40. O'Neill, K.R., S.H. Jones, and D.J. Grimes. 1992. Seasonal incidence of Vibrio vulnificus in the Great Bay Estuary of New Hampshire and Maine. Appl. Environ. Microbiol. 58:3257-3262.

41. Grimes, D.J., D. Jacobs, D.G. Swartz, P.R. Brayton and R.R. Colwell. 1993. Numerical taxonomy of gram-negative, oxidase-positive rods from carcharhinid sharks. Int. J. Syst. Bacteriol. 43:88-98.

42. Attaway, D.H. and D.J. Grimes. 1995. Perspectives on marine biotechnology in the United States government. J. Marine Biotechnol. 2:105-107.

43. Grimes, D.J., C.R. Woese, M.T. MacDonell and R.R. Colwell. 1997. Systematic study of the genus Vogesella gen. nov. and its type species Vogesella indigofera comb. nov. Int. J. Syst. Bacteriol. 47:19-27.

44. Harvell, C.D., K. Kim, J.M. Burkholder, R.R. Colwell, P.R. Epstein, D.J. Grimes, E.E. Hoffmann, E.K. Lipp, A.D.M.E. Osterhaus, R.M. Overstreet, J.W. Porter, G.W. Smith and G.R. Vasta. 1999. Emerging marine diseases - climate links and anthropogenic factors. Science 285:1505-1510.

45. Grimes, D.J., R.M. Atlas and S. Wald. 2001. Biological resource centers: OECD calls for developing a global network. ASM News 67:598-599.

46. Grimes, D.J. 2004. So many microbes, so little time, and so little money: needs and challenges in developing a global network of biological resource centers. Oceanography 17(3): 52-59.

47. Grimes, D.J. 2006. Koch’s postulates – then and now. Microbe 1:223-228.

48. Phillips, A.M.B., A. DePaola, J. Bowers, S. Ladner and D.J. Grimes. 2007. An evaluation of the use of remotely sensed parameters for prediction of incidence and risk associated with Vibrio parahaemolyticus in Gulf Coast oysters (Crassostrea virginica). J. Food Protection70:879-884.

49. Zimmerman, A.M., A. DePaola, J.C. Bowers, J.A. Krantz, J.L. Nordstrom, C.N. Johnson and D.J. Grimes. 2007. Variability of total and pathogenic Vibrio parahaemolyticus densities in northern Gulf of Mexico water and oysters. Appl. Environ. Microbiol.73:7589-7596.

50. Johnson, C.N., Y-J. Chang, A.D. Peacock, L. Kline, S. Barnes, J. Ogle and D.J. Grimes. 2008. Microbial community analysis of water, foregut and hindgut during growth of Pacific white shrimp, Litopenaeus vannamei, in closed-system aquaculture. J. World Aquacul. Soc.39:251-258.

51. Johnson, C.N., A.R. Flowers, V.C. Young, N. Gonzalez-Escalona, A. DePaola and D. J. Grimes. 2009. Genetic relatedness among tdh+ and trh+Vibrio parahaemolyticus cultured from Gulf of Mexico oysters (Crassostrea virginica) and surrounding water and sediment. Microb. Ecol. 57:437-443.

52. Zimmerman, A.M., D.M. Rebarchik, A.R. Flowers, J.L. Williams and D.J. Grimes. 2009. Escherichia coli detection using mTEC agar and fluorescent antibody direct viable counting on coastal recreational water samples. Lett. Appl. Microbiol. 49:478-483.

53. Grimes, D.J., C.N. Johnson, K.S. Dillon, A.R. Flowers, N.F. Noriea III and T. Berutti. 2009.What genomic sequence information has revealed about Vibrio ecology in the ocean – a review. Microb. Ecol. 58:447-460.

54. Noriea, N.F., III, C.N. Johnson, K.J. Griffitt and D. J. Grimes. 2010. Distribution of type III secretion systems in Vibrioparahaemolyticus from the Northern Gulf of Mexico. J. Appl. Microbiol. 109:953-962.

55. Johnson, C.N., A.R. Flowers, N.F. Noriea, III, A.M. Zimmerman, J. Bowers, A. DePaola and D.J. Grimes. 2010. Relationships between environmental factors and pathogenic vibrios in the northern Gulf of Mexico. Appl. Environ. Microbiol. 76:7076-7084.

56. Griffitt, K.J., N.F. Noriea III, C.N. Johnson and D.J. Grimes. 2011. Enumeration of Vibrio parahaemolyticus in the viable but nonculturable state using direct plate counts and recognition of individual gene fluorescence in situ hybridization. J. Microbiol. Meth.85:114-118.

57. Johnson, C.N., J.C. Bowers, K.J. Griffitt, V. Molina, R.W. Clostio, S. Pei, E. Laws, R.N. Paranjpye, M.S. Strom, A. Chen, N.A. Hasan, A. Huq, N.F.Noriea III, D.J. Grimes and R.R. Colwell.2012. Ecology of Vibrio parahaemolyticus and Vibrio vulnificus in the coastal and estuarine waters of Louisiana, Maryland, Mississippi, and Washington(United States). Appl. Environ. Microbiol. 78:7249-7257.

58. Griffitt, K.J. and D.J. Grimes. 2013. Abundance and distribution of Vibrio cholerae, V. parahaemolyticus, and V. vulnificus following a major freshwater intrusion into the Mississippi Sound. Microb. Ecol.65:578-583.DOI 10.1007/s00248-013-0203-659.

59. Griffitt, K.J. and D.J. Grimes. 2013. A novel agar formulation for isolation and direct enumeration of Vibrio vulnificus from oyster tissue. J. Microbiol. Meth. 94:98-102. doi: 10.1016/j.mimet.2013.04.0121.

60. Berutti, T.R., R.E. Williams, S. Shen, M.M. Taylor and D.J. Grimes. 2014. Prevalence of urease in Vibrio parahaemolyticus from the Mississippi Sound. Lett. Appl. Microbiol. 58:624-628. doi: 10.1111/lam.12237

61. Grimes, D.J., T.E. Ford, R.R. Colwell, C. Baker-Austin, J. Martinez-Urtaza, A. Subramaniam and D.G. Capone. 2014. Viewing marine bacteria, their activity and response to environmental drivers from orbit. Microb. Ecol. 67:489-500. doi: 10.1007/s00248-013-0363-4

62. Hasan, N.A., T. Rezayat, P.J. Blatz, S.Y. Choi, K.J. Griffitt, S.M. Rashed, A. Huq, N.G. Conger, R.R. Colwell and D.J. Grimes. 2015. Nontoxigenic Vibrio cholerae non-Ol/O139 isolate from a case of human gastroenteritis in the U.S. Gulf Coast. J. Clin. Microbiol.53:9-14. doi: 10.1128/JCM.02187-14

63. Torralba, M.G., J. Franks, A. Gomez, S. Yooseph, K.E. Nelson and D.J. Grimes. 2017. Effect of Macondo Prospect 252 oil on microbiota associated with pelagic Sargassum in the northern Gulf of Mexico. Microb. Ecol. 73:91-100. doi:10.1007/s00248-016-0857-y

Publications (journal articles in preparation and/or submitted):

Russo, C.D., M. Torralba, K.E. Nelson, D. Revie, J. Badger, R.S. Wellsand D.J. Grimes. 2017. A viromic study of serum collected from wild Tursiops truncatus (bottlenose dolphin) and report of the first marine system lentivirus with high relatedness to HIV-1. (mss. in preparation for Lancet)

Russo, C.D., D. Weller, K.E. Nelson, S. Chivers, M. Torralba and D.J. Grimes. 2017. (mss. submitted, reviewed and resubmitted to Microb. Eccol.)

Russo, C.D., R. Griffitt, K. Lefebvre, M. Torralba, K.E. Nelsonand D.J. Grimes. 2017. Bacteriomic and transcriptomic regulation in Zebrafish exposed to varying concentrations of the harmful algal bloom toxin domoic acid. (mss. in preparation for )

Russo, C.D. and D.J. Grimes. 2017. A review of viral species reported to be infectious for Tursiops truncatus (bottlenose dolphin). (submitted, reviewed, in revision)

Shen, S., M. Taylor, S.L. Allen, H. Nguyen, R.C. Prince and D.J. Grimes. 2017. Hydrocarbon degradation by Vibrios. (mss. in preparation)

Shen, S. and D.J. Grimes. 2017. Vibrio dioxygenases. (mss. in preparation)

Grimes, D.J., S. Shen, A.R. Flowers, A. Bardon-Albaret, R.B. Blaylock and J.M. Lotz. 2017. Vibrio vulnificus mortality in cultured red snapper. (mss. in preparation for J. Aquatic Animal Health)

Publications (books, chapters, proceedings, newsletters & book reviews):

1. Burns, R.M. and D.J. Grimes. 1979. Laboratory Manual for General Bacteriology. Gorsuch Scarisbrick, Publ., Dubuque.

2. Colwell, R.R. and D.J. Grimes. 1983. Microbiological/chemical hazards to divers in polluted waters. In Protection of Divers in Waters Containing Hazardous Chemicals, Pathogenic Organisms, and Radioactive Materials, Undersea Medical Society, Publ. No. CR60 (CW), Bethesda, MD.

3. Singleton, F.L., D.J. Grimes and R.R. Colwell. 1984. Autochthony of bacterial communities in ocean surface waters as an indicator of pollution, p. 443-469. In H. White (ed.), Concepts in Marine Pollution Measurements. Maryland Sea Grant College, University of Maryland, College Park.

4. Singleton, F.L., D.J. Grimes, H.-S. Xu and R.R. Colwell. 1985. Microbiological studies of ocean dumping, p. 187-208. In D.R. Kester, W.V. Burt, J.M. Capuzzo, P.K. Park, B.H. Ketchum, and I.W. Duedall (ed.), Wastes in the Ocean, Vol. 5, Deep-Sea Waste Disposal. John Wiley & Sons, Inc., New York.

5. Grimes, D.J. 1985. Microbiological studies of municipal waste release to aquatic environments, p. 270-276. In P.J. Godfrey, E.R. Kaynor, S. Pelczarski, and J. Benforado (eds.), Ecological Considerations in Wetlands Treatment of Municipal Wastewaters. Van Nostrand Reinhold Co., New York.

6. Colwell, R., P. Brayton, M. Tamplin, S. Huq, M. Voll, D. Roszak, S. Muralidhar, C. Somerville and J. Grimes. 1985. Pathogenic potential, including production of cholera toxin, in viable but not recoverable Vibrio cholerae 01 in environmental microcosms. In: Proceedings of 21st Joint Conference on Cholera, National Institutes of Health, Bethesda, Maryland, October, 1985.

7. Colwell, R.R. and D.J. Grimes. 1986. Evidence for genetic modification of microorganisms occurring in natural aquatic environments, p. 222-230. In T.M. Poston and R. Purdy (ed.), Aquatic Toxicology and Environmental Fate, 9th vol., ASTM Special Technical Publ. 921, American Society for Testing and Materials, Philadelphia.

8. Okpokwasili, G.C., D.J. Grimes and R.R. Colwell. 1986. The role of plasmids in degradation of phenanthrene by bacteria isolated from the estuarine environment, p. 2-8. In S. Barry, D.R. Houghton, G.C. Llewellyn, and C.E. O'Rear (ed.), Biodeterioration 6. C.A.B. Inter-national, Farnham Royal, Slough, UK.

9. Okpokwasili, G.C., C.C. Somerville, D.J. Grimes and R.R. Colwell. 1986. Plasmid-associated phenanthrene degradation by Chesapeake Bay sediment bacteria, p. 601-610. In Deuxieme Colloque International de Bacteriologie Marine - CNRS, IFREMER, Acte de Colloques, 3, Brest, France.

10. Brayton, P.R., D.B. Roszak, L.M. Palmer, S.A. Huq, D.J. Grimes and R.R. Colwell. 1986. Fluorescent antibody enumeration of Vibrio cholerae in the marine environment, p. 507-514. In Deuxieme Colloque International de Bacteriologie Marine - CNRS, IFREMER, Acte de Colloques, 3, Brest, France.

11. Grimes, D.J. 1986. Human pathogens associated with elasmobranchs and other marine animals, p. 417-421. In F. Megusar and M. Gantar (eds.), Perspectives in Microbial Ecology, Proceedings of the Fourth International Symposium on Microbial Ecology, Ljubljana, Yugoslavia.

12. Grimes, D.J., C.C. Somerville, W. Straube, D.B. Roszak, B.A. Ortiz-Conde, M.T. MacDonell and R.R. Colwell. 1988. Plasmid mobility in the ocean environment, p. 37-42. In W.J. Adams, G.A. Chapman, and W.G. Landis (ed.), Aquatic Toxicology and Hazard Assessment, l0th vol., ASTM Special Technical Publ. 971, American Society for Testing and Materials, Philadelphia.

13. Grimes, D.J. and R.R. Colwell. 1989. Ocean discharge of industrial and domestic wastewater at Barceloneta, Puerto Rico: bacteriological considerations, (p. 139-148). In D. Hood, A. Schoener, and P.K. Park (eds.), Ocean Processes in Marine Pollution, Vol. 4, Robert E. Krieger Publ. Co., Malabar, FL.

14. Grimes, D.J. 1990. Review of human pathogenic bacteria in marine animals with emphasis on sharks, p. 63-69. In H.L. Pratt, S.H. Gruber, and T. Taniuchi (eds.), Elasmobranchs as Living Resources: Advances in Biology, Ecology, Systematics, and the Status of the Fisheries. NOAA Tech. Rep. NMFS 90.

15. Grimes, D.J. and R.R. Colwell. 1990. Estuarine science and public health. Marine Division, National Association of State Universities & Land-Grant Colleges, Washington, D.C.

16. O'Neill, K.R., S.H. Jones, T.L. Howell and D.J. Grimes. 1991. Occurrence of Vibrio vulnificus in water and shellfish from Maine and New Hampshire, p. 189-193. In W.S. Otwell, G.E. Rodrick, and R.E. Martin (eds.), Molluscan Shellfish Depuration. CRC Press, Inc., Boca Raton, FL.

17. Grimes, D.J. 1994. Issues and opportunities in bioremediation research. Proceedings of the Fallen Leaf Lake Conference on Microbial Ecology and Biological Control. Molec. Ecol. 3:617-618.

18. Grimes, D.J. and G. Heufelder. 1994. Pathogen issues in Buzzards Bay, p. 1-58. In J.E. Costa, V. Gibson and J.M. Pederson (eds.), A Synthesis of Pollutant Inputs to Buzzards Bay. Buzzards Bay Project, Technical Report Series (BBP 94-30). U.S. Environmental Protection Agency and Massachusetts Executive Office of Environmental Affairs, Boston, MA.

19. Grimes, D.J. 1995. Culture collections and nonculturable cells. USFCC Newsletter 25(1):1-3.

20. Grimes, D.J. 1995. Validation of reliability and predictability of environmental biotechnologies, p. 309-315. In H. Uchida (Chair), Bioremediation: the Tokyo '94 Workshop (Proceedings of an OECD Workshop held in Tokyo, Japan, November, 1994). Organisation for Economic Co-operation and Development, Paris, France.

21. Grimes, D.J. 1997. Environmental biotechnology issues in the federal government, p. 147-152. In G.S. Sayler, J. Sanseverino, and K.L. Davis (eds.), Biotechnology in the Sustainable Environment. Plenum Publ. Corp., New York.

22. Grimes, D.J. 1998. Biotechnology and the environment, p. 731-741. In A. Altman (ed.), Agricultural Biotechnology. Marcel Dekker, Inc., New York.

23. Grimes, D.J. 1999. Forum: Water we count on. New Scientist 161(2171):46.

24. Colwell, R.R. and D.J. Grimes (eds.). 2000. Nonculturable Microorganisms in the Environment. ASM Press, Washington, D.C.