Appendix for ONLINE PUBLICATION ONLY

Appendix for ONLINE PUBLICATION ONLY

Appendix FOR ONLINE PUBLICATION ONLY

Appendix Figure A1: Temporal patterns of soil CO2emissions, soil temperature and soil moisture. A: Average of all soil CO2emission, soil temperature and soil moisture measurements collected during a sampling week (regardless of treatment or vegetation type). B: Relationship between soil CO2emissions and soil temperature, C: relationship between soil CO2emission and soil moisture. Note that one soil moisture value for 2009 is not available as the TDR was broken. Dates represent start of a sampling week; data collection for all the 18 sites was completed within three to five days (depending on weather conditions) for each measuring campaign.

Appendix Figure A1

Appendix Figure A2: Treatment effects for the two different vegetation types separately. A) soil temperature, B) soil moisture, C) root biomass, and D) soil MBC. Values represent means ± standard error. Normal font letters indicate differences between the short-grass treatments; bold letters indicate differences for the tall-grass treatments. Different letters represent significantly different values (α = 0.05).

Appendix Figure A2

Appendix Table A1: Comparison of the Impact of Exclosure Construction versus Invertebrate Exclusion on Different Ecosystem Parameters Measured

Parameters measured / None / Micro-climate control / Invertebrates / F / p
Roof / Roof / No roof
No invertebrates / Invertebrates / Invertebrates
Aboveground biomass (g m-2) / 602.24 (50.05)a / 442.12 (49.99)b / 337.75 (30.54)b / 12.46 / <0.001
Max vegetation height (cm) / 47.39 (2.15)a / 31.53 (2.77)b / 32.95 (1.95)b / 19.06 / <0.001
Mean vegetation height (cm) / 15.21 (0.90)a / 7.24 (0.58)b / 9.08 (0.62)b / 33.28 / <0.001
Soil moisture (%) / 36.42 (1.30)a / 31.36 (1.37)b / 34.30 (1.46)b / 4.64 / 0.010
Air humidity (%) / 72.29 (0.49)a / 71.27 (0.82)a / 70.49 (0.72)a / 2.26 / 0.123
Air temperature (˚C) / 10.85 (0.17)a / 10.94 (0.29)a / 11.08 (0.25)a / 0.27 / 0.762
Soil temperature (˚C) / 12.36 (0.25)b / 12.77 (0.27)ab / 13.17 (0.27)a / 3.54 / 0.030
PAR (μmol m-2 s-1) / 611.70 (51.42)b / 712.10 (57.21)ab / 903.16 (72.24)a / 6.62 / 0.002
UV (μmol m-2 s-1) / 37.99 (3.10)b / 42.04 (3.03)b / 56.71 (4.13)a / 8.49 / <0.001

For detailed description of the “None“,”Micro-climate control“ and“Invertebrates“ exclosures see text. Values represent means (and SE) of all measurements collected. Superscript letters represent statistically different values (α = 0.05).

Appendix Table A2: Average Number of Ungulate Pellet Groups 100 m-2, Number of Marmots Counted, and Average Number of Grasshoppers m-2 at Each Site for Summer 2009 and 2010

Pellet groups 100 m-2 / Marmots site-1 / Grasshoppers m-2
Site / Vegetation type / 2009 / 2010 / 2009 / 2010 / 2009 / 2010
1 / Short-grass / 13.7 / 12.4 / 5.0 / 1.0 / 0.5 / 5.3
2 / Tall-grass / 6.0 / 8.3 / 5.0 / 1.0 / 0.7 / 5.0
3 / Short-grass / 9.3 / 12.1 / 5.0 / 1.0 / 0.5 / 1.5
4 / Tall-grass / 7.7 / 10.1 / 5.0 / 1.0 / 0.9 / 5.9
5 / Short-grass / 17.8 / 11.9 / 3.0 / 2.0 / 0.6 / 2.7
6 / Tall-grass / 8.3 / 8.5 / 3.0 / 2.0 / 0.9 / 3.8
7 / Short-grass / 1.8 / 8.9 / 9.0 / 1.0 / 0.2 / 0.4
8 / Tall-grass / 15.2 / 10.6 / 9.0 / 1.0 / 0.6 / 1.6
9 / Short-grass / 1.7 / 4.4 / 2.0 / 2.0 / 0.1 / 1.0
10 / Tall-grass / 6.0 / 9.4 / 2.0 / 2.0 / 0.2 / 0.3
11 / Short-grass / 13.2 / 13.2 / 17.0 / 21.0 / 0.5 / 2.8
12 / Tall-grass / 15.2 / 21.0 / 17.0 / 21.0 / 1.6 / 4.4
13 / Short-grass / 17.3 / 15.0 / 17.0 / 21.0 / 1.1 / 3.6
14 / Tall-grass / 26.0 / 13.6 / 17.0 / 21.0 / 0.8 / 3.5
15 / Short-grass / 20.3 / 6.9 / 11.0 / 11.0 / 0.5 / 0.4
16 / Tall-grass / 9.2 / 6.5 / 11.0 / 11.0 / 0.2 / 0.1
17 / Short-grass / 28.3 / 14.2 / 11.0 / 11.0 / 0.3 / 0.7
18 / Tall-grass / 10.5 / 5.6 / 11.0 / 11.0 / 0.3 / 0.3

Appendix table A3: Statistical Results from the Linear Mixed Model Approach

Model / Numerator df / Denominator df / F / p
Short-grass vegetation
Intercept / 1 / 0.752 / 14.425 / 0.225
Year / 1 / 515.902 / 105.209 / <0.001
Treatment / 4 / 515.902 / 5.276 / <0.001
Year × treatment / 4 / 515.902 / 0.269 / 0.898
Sampling date / 6 / 515.902 / 22.382 / <0.001
Tall-grass vegetation
Intercept / 1 / 0.009 / 1.400 / 0.971
Year / 1 / 512.622 / 119.093 / <0.001
Treatment / 4 / 512.622 / 7.261 / <0.001
Year × treatment / 4 / 512.622 / 0.471 / 0.757
Sampling date / 6 / 512.622 / 39.196 / <0.001

df = degrees of freedom