SupplementalTable 1. Protein Identities and Their Relative Changes under Cd Stress in Kandelia candel roots from 2D-Gel Analysis

Spot noa / Matched peptide sequences (m/z) / Matched peptides / MP/SCb
Protein metabolism: protein synthesis, protein folding and proteolysis
Protein folding
28 / ELPNAYVFVIDMPGLK(1821.9772)
SSHGPTR(741.3542)
AMAATPADVK(974.4849)
VMGLDSPLFSTLQHMIDAGNEEAEK(2764.4099) / 4 / 4 / 70
29 / ILQISGER (915.5171)
ADLPGLK(713.4171)
VEVEEGR(817.3918)
NDKWHR(855.4162)
FRLPENAK(974.566)
ETPEAHVFK(1057.5416)
FDWKETPEAHVFK(1633.8182) / 7 / 7 / 41
Protein synthsis
2 / LWGENFFDPATR (1452.7584)
GHVFEEMQRPGTPLYNIK (2132.2358)
FFAFGR (744.4054)
FSVSPVVR (890.5389)
SDPVVSFR (906.5015)
EVDAHPIR (936.5183)
GGGQVIPTAR (955.5659)
LWGENFFDPATRK (1580.8729)
NATLTNEKEVDAHPIR (1808.0537) / 9 / 9 / 12
3 / GHVFEELQRPGTPLYNIK (2098.2498)
FFAFGR (744.4052)
FSVSPVVR (890.541)
SDPVVSFR (906.5016)
EVDAHPIR (936.5184)
GGGQVIPTAR (955.5723)
NATLTNEKEVDAHPIR (1808.0295)
STLTDSLVAAAGIIAQEVAGDVR (2257.3704)
QGNEYLINLIDSPGHVDFSSEVTAALR(2945.7419) / 9 / 9 / 14
21 / DYGEESEGLNVVQQHIK (1945.0042) / 1 / 1 / 12
22 / GFGFVTFNNEK (1259.5973)
MSADIEYR (1000.4999)
GGGESDGNWKN (1120.5516)
NITVNEAQSR (1131.561)
SRGFGFVTFNNEK (1502.7352)
SMRDAIQGMNSQELDGR (1908.0511) / 6 / 6 / 36
45 / TYPQQAGTIR (1134.6379)
VVEVSTSKTGK (1134.6379)
LPTDDNLLTQIK (1370.8173)
GRPCKVVEVSTSK (1389.7277)
DDLRLPTDDNLLTQIK (1870.0563)
TDYQLIDISEDGFVSLLTENGNTK (2672.4792) / 6 / 6 / 47
Proteolysis
12 / LVVAPPNFVIK (1196.7235)
EYAWR (724.3215)
GELATALR (830.4543)
IVDKDGAR (873.4604)
VQFTEYIQK (1155.5874)
NGIPLSTAAAANFTR (1503.827)
GAFGYGSYFSLAMMDR (1772.7328) / 7 / 7 / 36
13 / SWTGTIIGPHNTVHEGR (1861.903)
LLEELER(901.4699)
EMAAPHNR(941.4017)
FGLLVNWQR(1132.5671)
LVQPPEGTYF(1150.6045)
DYPEKPPSVR(1187.5699)
LLEELERGEK(1215.6318)
KLVQPPEGTYF(1278.6766)
EYTMEDILTQLK(1499.7063)
EYTMEDILTQLKK(1627.8176)
GIGDGTVSYGMDDGDDIYMR(2153.0139) / 11 / 11 / 67
19 / NHLLPNPR (960.5216)
TYINPK(735.3813)
GHKEYLR(902.4568)
YREGLNR(907.4599)
VITALTLLK(960.5216)
EGLNRDEGIK(1130.567) / 6 / 6 / 15
44 / YNGGLETEEAYPYTGK (1791.9934) / 1 / 1 / 7
50 / HITIFSPEGR (1156.6309)
LLDQTSVTHLFPITK (1712.9999)
GSGGGYDR(768.3319)
TLVQQAR(815.4802)
NEAAEFR(836.398)
LFQVEYAFK(1144.6239)
EQEAINFLEK(1220.6418)
YLGLLATGMTADAR(1452.7994)
ATSAGLKEQEAINFLEK(1848.9635) / 9 / 9 / 42
Energy and carbohydrate metabolism
37 / AIIGKSIPLR (1067.6869)
KLEEVR(773.473) / 2 / 2 / 8
39 / VVDGLGVPIDGR (1196.704)
EVAAFAQFGSDLDAATQALLNR (1537.7957)
EAFPGDVFYLHSR (2308.2524)
QAVAYR(707.4092)
APGIIER(755.4629)
ELIIGDR(815.4863)
EGDLVKR(816.4935)
QMSLLLR(876.5275)
GALSDHER(884.4586)
LELAQYR(892.5234)
VVSVGDGIAR(972.5801)
AVDSLVPIGR(1026.6328)
VGSAAQLKAMK(1103.5441)
SVHEPMQTGLK(1242.6615)
QPQYAPLPIEK(1283.7479)
TAIAIDTILNQK(1300.811)
GIRPAINVGLSVSR(1438.8938)
AMLGRVVDGLGVPIDGR(1724.9622) / 18 / 18 / 42
43 / VVDLLAPYQR (1173.7021)
VGLTGLTVAEHFR (1399.813)
FTQANSEVSALLGR (1492.8171)
EGNDLYR(866.4281)
AHGGFSVFAGVGER(1390.7246)
AHGGFSVFAGVGERTR(1647.9658)
QISELGIYPAVDPLDSTSR(2061.1165)
NLQDIIAILGMDELSEDDKLTVAR(2688.4746) / 8 / 8 / 25
36 / IIGATNPADSAPGTIR (1553.8843)
NVVVTGR(744.4657)
KIIGATNPADSAPGTIR(1681.9858) / 3 / 3 / 28
52 / KVEFGLPDLESR (1389.7906)
FELLAR(748.4656)
YVGEGAR(751.409)
ELFQMAR(910.4898)
VEFGLPDLESR(1261.7137)
FDDGVGGDNEVQR(1407.6675)
TMLEIVNQLDGFDAR(1737.9554)
MREVVELPMLHPEK(1739.9673) / 8 / 8 / 34
1 / YYSLPALNDPR (1308.7255)
VHPLTR(722.4583)
LPYSIR(748.4611)
GTFANIR(778.4533)
ILLESAIR(914.6086)
DFNSYGSR(945.4512)
QVEIPFKPAR(1184.7386)
MFVDYSEPQIER(1513.7755)
SDETVSMIESYLR(1529.7996)
DGVTATDLVLTVTQMLR(1849.0972)
FVEFYGEGMSELSLADR(1966.0505)
SEGHDTVILAGAEYGSGSSR(1993.0845)
VLLQDFTGVPAVVDLACMR(2047.2061)
YKSEGHDTVILAGAEYGSGSSR(2284.2314)
INPLVPVDLVIDHSVQVDVAR(2298.4348) / 15 / 15 / 15
4 / EAQNAAFVLVAGGLGER (1701.9681)
LNIGGDFASSLPSLHK (1655.9216)
DIPFVIMTSDDTHAR (1717.9591)
AIPASLGVSATKQYHVNSLAVPR (2379.406) / 4 / 4 / 11
5 / RPQGLYISLK (1174.7533)
GLIYPPFSNIR (1276.7764)
MESTLK (724.3893)
LMNSIR (733.4261)
YGSIFR (742.4142)
LMNSIR (749.425)
QYQVPLQK (1003.5777)
KPWAHEHEPVK (1357.8088)
DIRDGASVLDLDPK (1513.8564)
VIKPTVLIGSSGVGKAFTK (1902.0551) / 10 / 10 / 10
7 / GEMLDLQHAAAFLPR (1684.946)
GYTSWAIGYSAANLAR (1700.9354)
LNLLQR (756.4901)
QIPGESR (786.4359)
LSGFPSNR (877.4791)
IHPVSVLAK (963.5626)
NVTLFKNIIPPLAK (1567.8308)
GFYGIDGGDVFLSLPAQLGR (2082.2578) / 8 / 8 / 28
16 / GFPISVYNR (1052.5353)
DYFGAHTYER (1258.5355)
AVFLDR (720.3875)
GGSGNFVK (765.3433)
FLSGLKEER (1078.5741)
LPANLVQAQR (1109.6244)
ICSYAQGMNLIR (1368.6831)
ERLPANLVQAQR (1394.761) / 8 / 8 / 14
24 / YLFEDGSR (986.4347)
SIFDFESIR (1113.5358)
VVHGDEFEYNDPVDGSISSHQGIR (2657.2952)
HYYTR (739.3337)
GATLVVSGDGR (1031.5215)
LYIEQYEK (1085.5303)
FFEVPTGWK (1110.5428)
LSGTGSEGATIR (1148.5813)
LSKMEEFTGR (1213.6042)
SIFDFESIRK (1241.6356)
LYIEQYEKDPSK (1512.7285)
EDFGGGHPDPNLTYAK (1717.7812)
LSHEALAPLVEAALKLSK (1889.9482)
LVSVEDIVRQHWATYGR (2029.08) / 14 / 14 / 26
30 / VVPGFGHGVLR (1137.6783)
YWEPTYEDSLNLIAR (1869.9242)
ALGLPLERPK (1093.6962)
HLPDDPLFQLVSK (1508.8362)
GMTGLLWETSLLDPDEGIR (2119.0667) / 5 / 5 / 15
33 / LFGVTTLDVVR (1219.7314)
MSPLVSALHLYDIANVK (1887.0508)
KPGMTR (705.4056)
ANTFVAQK (878.4916)
KLFGVTTLDVVR (1347.826)
DDLFNINANIVK (1375.7441)
AGAGSATLSMAYAAAR (1484.7571)
VAILGAAGGIGQPLALLVK (1761.1547)
LIDVDVPVVGGHAGITILPLLSK (2326.4001) / 9 / 9 / 24
35 / VLVTGAAGQIGYALVPMIAR (2016.2155)
ALGQISER (873.5113)
TPSGEKPVR (970.5686) / 3 / 3 / 21
49 / VLVTGAAGQIGYALVPMIAR(2016.3203)
NVSIYK (723.4177)
EGMERK (765.379)
SQASALEK (833.4861)
ALGQVSER (859.4993)
VLVVANPANTNALILK (1650.097)
NVIIWGNHSSTQYPDVNHATVK (2480.2605)
KPVRVLVTGAAGQIGYALVPMIAR (2480.2605) / 8 / 8 / 27
Detoxification and antioxidation
32 / EHIAAYGEGNER (1345.6841)
GYFEDR(786.3666)
HKEHIAAYGEGNER(1610.8218)
HETANISTFKWGVANR(1830.9202) / 4 / 4 / 10
42 / EHIAAYGEGNER (1345.6547)
HREHIAAYGEGNER (1638.8203)
GYFEDR(786.363)
EHIAAYGEGNERR(1501.777) / 4 / 4 / 6
6 / AYLFPEGTAR (1124.6138)
LYNDIR(793.4417)
NIFYLR(825.4873)
LYNDIRR(949.5722)
VEHVDHAR(962.5355)
RVEHVDHAR(1118.6456)
VLEADIVVVGVGGRPLTTLFK(2183.3972) / 7 / 7 / 12
18 / YAADEDAFFADYAEAHLK(2046.9683)
DVFGAQMGLSDKDIVALSGGHTLGR(2560.4238)
DIVALSGGHTLGR(1295.6724)
IAWHSAGTYDVK(1347.676) / 4 / 4 / 22
46 / QGLFTSDQDLYTDSR (1745.8829)
FVVAMIK(807.4747)
DSVVLSGGPKYQVALGR(1745.8829) / 3 / 3 / 11
51 / KLPIHHIPGPEGVR (1549.9509)
ISITGAGGFIASHIAR (1570.9242)
INGIKR(700.4361)
EQYWPSENLK(1293.6559)
LPIHHIPGPEGVR(1421.8562)
VVGTQAPVQLGSLR(1424.8684)
SFTFIDECVEGVLR(1614.8734)
LPIHHIPGPEGVRGR(1634.8998) / 8 / 8 / 20
23 / APLLPSDPYQR (1256.663)
IYDLGR(736.3903)
EQDLMNK(877.4543)
LMEWANR(919.4234)
GEEQEGAKK(975.493)
FWADFVDK(1027.4725)
SPLLLQMNPVHK(1392.7585)
DKAPLLPSDPYQR(1499.803) / 8 / 8 / 28
53 / FWADFIDKK (1169.6986)
FWADFIDK(1041.5959)
SSLLLEMNPVHK(1367.8551)
ELIEILKTLESELGDK(1829.965) / 4 / 4 / 17
40 / SINGLVLPTK(1041.5991)
NVLMGKLGDR(1118.5934)
QGAVVFEVQR(1132.5958)
SFADRIIGLR(1147.5875)
CGMVFQQHHLIGR(1525.8768)
RCGMVFQQHHLIGR(1681.983)
LEALTVIERVGLLEK(1682.967)
GVSVRYVDSTVALHPTSLDVK(2243.1411) / 8 / 8 / 37
25 / ELNQVPHVIELNER (1689.8881)
TVPQVFIDGK(1103.5951)
RTVPQVFIDGK(1259.6718) / 3 / 3 / 19
15 / SPHKTR(725.3546)
EKELDAR(860.5261)
APFPTIKMWR(1262.7058)
MAAAHSSRSTATR(1362.7703)
DFAKEPATYDGDVK(1555.8391)
EPATYDGDVKDLDAIK(1749.9473)
SRYNFVMFYAPWDGHS(2121.1353)
FEIEEYPTLVLFRDGVPK(2152.0627)
THQMAGTEVTFGLVDATREK(2191.0244)
LLDRLFQGPIAVNIFVFLPEER(2586.2981) / 10 / 10 / 25
20 / GNVQPEAVLQTVSK (1469.8192) / 1 / 1 / 35
31 / DQADYISVPIEGPYKPPHYR (2345.2529)
DGLKADPK(843.4724)
HSLPDGLMR(1025.5792)
ITIKPQTDR(1071.6718)
VKDMSQADFGR(1269.6635)
LVGVSEETTTGVKR(1475.8719)
RLYQMQANGTLLFPAINVNDSVTK(2693.481)
ITGSLHMTIQTAVLIETLTALGAEVR(2754.6221) / 8 / 8 / 25
34 / DQXDYVSIPIEGPYKPPHYR (2345.2529)
DSAAVFAWK(994.5468)
HSLPDGLMR(1025.5792)
ITIKPQTDR(1071.6718)
GCAAAMKTAGAR(1107.6068)
VKDMSQADFGR(1269.6635)
LVGVSEETTTGVKR(1475.8719)
TGQVPDPTSTDNPEFQIVLSIIK(2499.4082)
ITGSLHMTIQTAVLIETLTALGAEVR(2754.6221) / 9 / 9 / 32
38 / TAAYGHFGR (979.523)
TIFHLNPSGR (1141.6696)
FVIGGPHGDAGLTGR (1453.8308)
SGAYIVR(765.4619)
TNLVMVFGEITTK(1452.838)
ENFDFRPGMISINLDLK(2009.1265)
ENFDFRPGMISINLDLKR(2165.3086)
VLVNIEQQSPDIAQGVHGHLTK(2383.4072) / 8 / 8 / 24
Signal transduction
47 / DSTLIMQLLR(1205.7061)
SAQDIANAELAPTHPIR(1804.0283)
YLAEFK(770.4292)
LAEQAER(816.4493)
IISSIEQK(917.5622)
MKGDYHR(922.4554)
GNEDHVSLIR(1139.6722)
YEEMVEFMEK(1366.6185)
IISSIEQKEESR(1418.7595)
QAFDEAIAELDTLGEESYK(2129.1079)
LGLALNFSVFYYEILNSPDR(2331.3376) / 11 / 11 / 40
48 / DSTLIMQLLR (1205.7262)
NVIGSLR(758.488)
YLAEFK(770.4437)
LAEQAER(816.4622)
IVSSIEQK(903.5712)
MKGDYHR(922.4756)
YEEMVQFMQK(1364.6643)
IVSSIEQKEEGR(1374.7894)
LVLGSTPSGELTVEER(1686.9866) / 9 / 9 / 30
Other proteins
17 / MDFIPGIDGPSEGVPR (1702.8303)
IQFHR(700.4365)
INIKNDSR(959.4656)
EFYGEGYDDSDKR(1580.6471)
TSLWDLLESXLTYQHR(1948.978) / 5 / 5 / 15
41 / TIVGWAAR (873.5388)
DPSGILSPYTYTLR (1582.8796)
FVVDVAGSKLEP(1260.6381) / 3 / 3 / 10
10 / VQELSVYELNER (1478.8348)
GSPAYLR(763.4334)
DRGSPAYLR(1034.5839)
VQELSVYELNERDR(1749.9878) / 4 / 4 / 9
11 / LQQIVFPFK (1119.7054)
GSPAYLR(763.4334)
DRGSPAYLR(1034.5839)
SVNSLGDLVPFSNK(1476.8654) / 4 / 4 / 13
26 / SALDYLELQPDLSALVR (1903.0155)
LYIATDGR(908.4623)
MDDNYLR(926.3909)
ALVPFYPMAGR(1221.6062) / 4 / 4 / 8
27 / SALDYLEIQPDLSALVR (1903.0155)
MDNDYLR(926.3909)
ITPEQLSQLKAK(1355.7079) / 3 / 3 / 10
8 / GFTVSTVGEEMLYNPR (1799.951)
SIISKMSK(893.4717)
LTKDEETFK(1110.6007)
MAFTGDALLIR(1207.6907) / 4 / 4 / 17
9 / ITAGITDLIDVHFNVEK (1885.1412)
ITAGITDLIDVHFNVEKR (2041.248)
SAGLPWTVAK(1029.6235)
GQDTFTPISSVLSK(1479.8501) / 4 / 4 / 19

a Number of each protein spot of 2-DE.

b The number of matched peptides and the percentage of predicated protein sequence covered by matched sequence.