B7 Biology Across the Ecosystem Advance Planning Page 1

B7 Biology across the ecosystem • advance planning page 1

TWENTY FIRST CENTURY SCIENCE B7 Biology across the ecosystemTechnicians’ advance planning sheets

This table shows items that may need to be ordered in advance for this module. It does not list standard items, for example, test tubes, OHT pens etc.

Full details of the resources needed for each activity are given in the Requirements section of the Teacher & Technician notes, which also include safety information for the activity.

Activity / Item(s) / Quantity per student / group of students, or for the teacher / Notes
AB7.1 / Elodea
Microscope with a 40x objective / Per group / Elodea is used by the leaf rather than the whole plant so less is needed
AB7.2 / Geranium
Potassium hydroxide
Ethanol
Iodine solution
Limewater / Per group / Geranium needs to be destarched for at least 24 hours before experiment
AB7.3 / Molymod models / Per group / Sufficient models to make 6 carbon dioxide and
6 water molecules
AB7.4 / Young nettle or spinach leaves
Chromatography paper
Hairdryer (optional)
Petroleum ether (all boiling points)
Acetone (propanone) / Per group / The petroleum ether and the acetone are to produce a chromatography solvent. The lower boiling point mixtures give better results but are more flammable
AB7.7 / Visking tubing
Capillary tubing
Starch solution
Glucose solution / Per group / Visking tubing needs to soaked at least an hour before use
AB7.8 / Elodea
Sodium hydrogen carbonate
Lamp
Light meter (optional) / Per group / Cabomba which can be obtained from aquarium shops works as well if not better than Elodea
Audus micropipettes can be used in this experiment
AB7.9 / Datalogging software & relevant computer equipment
Light sensor
Oxygen sensor
pH sensor (optional)
Temperature sensor (optional)
Magnetic stirrer
Elodea
Sodium hydrogen carbonate / Per class / You need at least 6-12 sprigs of Elodea
IB7.4 / Computer access with Excel / Per group
AB7.12 / Tin lid
Gas jar and lid
Damp soil
Digital balance
(1 decimal place is acceptable) / Per group
AB7.18 / Yeast suspension
Sucrose
Lactose
Fructose
Yeast nutrient
Phenolphthalein solid
Ethanol
Sodium hydroxide
Liquid paraffin
Alkaline phenolphthalein solution / Per group / 1g of Yeast nutrient can be replace by 0.5g of ammonium dihydrogen orthophosphate and 0.5g of ammonium sulphate
Dataloggers, magnetic stirrers and bubble counters may be used
AB7.19 / Bacterial transformation kit
Access to incubator
Small polystyrene container of crushed ice
If pGLO kit used, access to long wave UV light / Per group / The Bacterial transformation kits are available from Bio-Rad or the NCBE. Details are in the C21 Teacher & Technician guide for B7.
AB7.21 / Lambda DNA protocol or DNA Fingerprinting Kit
Gel electrophoresis tanks
Micropipettes
Other laboratory materials as required by kits / Per group / The kits are available from
NCBE (lambda DNA)
Bio-Rad (DNA fingerprinting)
Economatics (DNA fingerprinting)
Some kits contain gel electrophoresis tanks and micropipettes
AB7.27 / Metronome
Access to stairs or 30cm high bench / Per class
Per pair/group
AB7.29 / ATP solution in a 1cm syringe
1% glucose in 1cm syringe
Distilled water in a 1cm syringe
Strips of fresh pork or beef
Dissecting needle / Per class
Per group / Meat needs to be very fresh and cut parallel to the fibres, so that each group has a 1cm wide by 3cm long strip
AB7.30 / Chicken wings
Disposable plates
Dissecting scissors
Blunt probe / Per group / Raw chicken should be used