Supplementary Table 1. Primers Used for the Different Experiments

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Supplementary Table 1. Primers used for the different experiments.

Primers used for the generation of probes for the RNA in situ hybridizations. The T7 RNA polymerase binding sites are underlined.
TaVRN1T7-F / 5’- GCGAAATTAATACGACTCACTATAGGGCGAAGCTGAAGGGCTTCCAGCCCATATAAG -3’
TaVRN1T7-R / 5’- GCGAAATTAATACGACTCACTATAGGGCGAATACATGGTAAATTGAGCCCAGCTGGG -3’
Primers used for the generation of bait constructs used for the two-hybrid screening.The restriction enzyme cutting sites used for cloning are underlined.
TaVRN1(IKC)-F / 5’- accatgggaTGGTGTCACGAATATAGG -3’
TaVRN1(IKC)-R / 5’- gaattcTCAGCCGTTGATGTGGCTAAC -3’
Primers used for RT-PCR analysis of genes identified by two-hybrid screening.
TaRLK- F / 5’- AGGTTCATGTGGTCGCTGAT -3’
TaRLKA- R / 5’- ATCCAAGACGCATGGAAGAG -3’
TaRNAb- F / 5’- CATCCATGCCCAAAGTTTCT -3’
TaRNAb-R / 5’- CATACCCACCTCACATGCAG -3’
TaCyclo- F / 5’- Tttctgaggtgacgcacaag -3’
TaCyclo-R / 5’- Tgccgtagattgattcacca -3’
TabHLH-F / 5’- Caccacaaccaccactaccc -3’
TabHLH-R / 5’- Ttcccgagtccaagtcctta -3’
TaWWRKY-F / 5’- Gaggccgagaagaaggtagg -3’
TaWRKY-R / 5’- Ctgcccgtacttcctccata -3’
TaVRN1-F2 / 5’- GCTGAAGGGCTTCCAGCCCATATAAG -3’
TaVRN1-R2 / 5’- TACATGGTAAATTGAGCCCAGCTGGG -3’
Ta18-F / 5’- AGTTAAAAAGCTCGTAGTTGGACCT -3’
Ta18-R / 5’- GTTTATGGTTGAGACTAGGACGGTA -3’
Primers used for the generation of the Pro35S:TaVRN1 and Pro35S:TaVRT2 constructs used to transform Arabidopsis. The restriction enzyme cutting sites used for cloning are underlined.
TaVRN1-xt-f / 5’- tAAGCTTATGGGGCGCGGGAAGGTGCA -3’
TaVRN1-xt-r / 5’- agaattcTCAGCCGTTGATGTGGCTAACCA-3’
TaVRT2-xt f2 / 5’- CTCTAGAtATGGCGCGGGAGAG-3’
TaVRT2-xt r2 / 5’- GAGCTCtCTTCCAAGGTAACGCTAGT-3’
Primers used for RT-PCR analyses of TaVRN1-overexpressing and TaVRT2-overexpressing Arabidopsis plants.
Actin3-F / 5’- GGCTCCAAGCAGCATGAAGATCAA-3’
Actin3-R / 5’- TGGCGGTGCTTCTTCTCTGAAAAAT-3’
AGL24-F / 5’- GAATGAGAGACATATTGGGAAGGTA -3’
AGL24-R / 5’- AAGTGTCGGAGTCATCCTCAAG -3’
AP1-F / 5’- ATTGCACCTGAGTCCGACGTCAATA -3’
AP1-R / 5’- TCATGCGGCGAAGCAGCCAAGGTT -3’
AXR1-F / 5’- TGGTCTCAGCTGAACTGTCAT -3’
AXR1-R / 5’- ATATTCTTCTTAGAGCTGCGG -3’
AXR3-F / 5’- CGATCCTTTCATGAGACGTAA -3’
AXR3-R / 5’- ACTCATGACGTCGTGACTTTT -3’
CAL-F / 5’- ACCTGACTCTCACGTTAATGCACAG -3’
CAL-R / 5’- TCAAGCGGCGTAACAGCCAAGGTAA -3’
FCA-F / 5’- AATGTACCTGGACCGAGCATACCT -3’
FCA-R / 5’- CTGCTGAACTTGTTGTGGTTGTTG -3’
FLC-F / 5’- GATCCTTGATCGATATGGGAAACAG -3’
FLC-R / 5’- TCTGCTCCCACATGATGATTATTCTCCAT -3’
FT-F / 5’- TAGTAAGCAGAGTTGTTGGAGACG -3’
FT-R / 5’- GGGAAGGCCGAGATTGTAGAT -3’
FUL-F / 5’- GATCGCTATTTATATTCAGACAAACAA -3’
FUL-R / 5’- ACTCGTTCGTAGTGGTAGGACGTAA -3’
FY-F / 5’- GCCAACCTGATAATTTCCAACCAT -3’
FY-R / 5’- ATGGAACCTGGAAGAGGCTGTTTA -3’
LD-F / 5’- CTTGATGAACGAAGAATTGCTGCT -3’
LD-R / 5’- AACTTCGACCCTTTCTTCAACCTG -3’
LFY-F / 5’- TCATTTGCTACTCTCCGCCGCT -3’
LFY-R / 5’- CATTTTTCGCCACGGTCTTTAG -3’
MAX1-F / 5’- GAGAAGACTAATGCAAGACTG -3’
MAX1-R / 5’- TAGAACTCCTAGTGCTAACCA -3’
MAX2-F / 5’- TAAACGTCTACACACGATCTC -3’
MAX2-R / 5’- TCAATGCCTCTAAAGCTACAC -3’
MAX3-F / 5’- gtccacgtcttcagctttcc -3’
MAX3-R / 5’- aatctgacggcttgttccac -3’
MAX4-F / 5’- ctcggaggatcttcggtgta -3’
MAX4-R / 5’- gggacaccactcgaacttgt -3’
SOC1-F / 5’- ACCATAGATCGTTATCTGAGGCAT -3’
SOC1-R / 5’- GAAGAACAAGGTAACCCAATGAAC -3’
SVP-F / 5’- GAAGGAAGTCCTAGAGAGGCATAAC -3’
SVP-R / 5’- CGTTAGTAATAGACTCCGACGACTG -3’
TaVRN1-F / 5’- GCTGAAGGGCTTCCAGCCCATATAAG -3’
TaVRN1-R / 5’- TACATGGTAAATTGAGCCCAGCTGGG -3’
TaVRT2-F / 5’- GGACCGGCAATTCATGCAACA -3’
TaVRT2-R / 5’- TCCTGCGAGCTTCCCGAATG -3’
Primers used for the generation of the TaVRN1 recombinant protein in E. coli. The restriction enzyme cutting sites used for cloning are underlined.
TaVRN1-F1 / 5’-AAGGATCCGATGGGGCGCGGGAAGGTGCAGCTGAAGCGGAT -3’
TaVRN1-R1 / 5’- GTGAATTCCCTTCAGCCGTTGATGTGGCT -3’