Michael A. Ewing
(last updated: July 3, 2015)
3003 E Amy Lane
Bloomington, IN 47408
(317) 308-9680
Research Interests:
-Physical, analytical, and biological chemistry
-Applying gas-phase techniques to study populations of conformations ofbiological molecules
-Understanding the relationship between conformations across phase-transitions
-Understanding biomolecule motion in the absence of solvent
-Development of methods for rapid determination of chirality, especially at single siteswithin large molecules containing multiple chiral centers
Education:
Ph.D. Analytical Chemistry, Expected August2015
Indiana University, Bloomington, IN
Advisor: Professor David Clemmer
M.S. Analytical Chemistry, May 2008
University of Illinois at Urbana-Champaign, Urbana, IL
Thesis: “Novel Detection and Measurement Methods for D-Amino Acid-Containing Peptides”
Advisor: Professor Jonathan Sweedler
B.S. Chemistry, May 2003
B.S. Mathematics (Statistics Concentration), May 2003
Minor in Applied Biology
Minor in Electrical and Computer Engineering
Rose–Hulman Institute of Technology, Terre Haute, IN
Graduated magna cum laude
Professional Experience:
Indiana University, Bloomington, IN
Graduate Student2010 – Present
- Developed high resolution ion mobility spectrometers
- Studied methods for predicting cross-section of biomolecules
- Examined energetics and kinetics of solution- and gas-phase structural transitions
Lancaster Laboratories, Indianapolis, IN
Chemist III2008 – 2010
- Trace analysis of genotoxic impurities using GC-MS and LC-MS
- Contract work for a large pharmaceutical company
University of Illinois, Urbana-Champaign, IL2003 – 2008
Graduate Student
- Studied neuropeptides of Aplysia californica
- Developed method to examine chirality of residues within neuropeptides
Rose-Hulman Institute of Technology, Terre Haute, IN
Resident Tutor2000 – 2003
- Tutored math, science, and engineering subjects as part of the learning center
- Focused on sophomore classes but acted as lead tutor for a wide range of classes
Rose-Hulman Institute of Technology, Terre Haute, IN
Student Researcher2000 – 2003
- Wrote a program for calculating energies of a double well potential
Rose-Hulman Institute of Technology, Terre Haute, IN
Teaching Assistant2002 – 2003
- Graded homework and quizzes, supervised labs and worked on a curriculum in general chemistry and advanced statistics courses
Indiana University, Bloomington, IN
REU ParticipantSummer 2002
- Developed a method to modify proteins to reduce charge state
- Processed and analyzed data for IMS-MS (IM-MS) experiments
University of Pittsburgh, Pittsburgh, PA
REU ParticipantSummer 2001
- Studied model potentials for molecular mechanics including water
Pennsylvania State University, University Park, PA
Research Assistant in ChemistrySummer 1998
- Constructed ultramicroelectrodes down to 110 nm in radius
- Wrote and troubleshot LabVIEW programs for instrument control
Honors:
ACS Division of Analytical Chemistry Graduate Research Fellowship
by Eastman Chemical CompanySummer 2013
Department of Chemistry Teaching Award2011
National Science Foundation Graduate Research Fellowship2005 – 2007
University of Illinois Fellowship2003 – 2005
Department of Chemistry Teaching Award2004
William Albert Noyes Sr. Award in Chemistry2003
Clarence P. Sousley Award (Senior Math Award)2003
Putnam Commendation2003
Roger Louis Maness Memorial Scholarship (Chem Scholarship)2002
Theodore Paine Palmer Award (Freshman Math Award)2000
Presidential Scholarship (Rose-Hulman Scholarship)1999 – 2003
Professional Memberships:
American Chemical Society
American Society for Mass Spectrometry
Teaching Assignments:
Indiana University
CHEM C589/BIOC B541 Lecture – Enzyme MechanismsSpring 2015
CHEM C588/BIOC B540 Lecture –Fundamentals of Biomolecular CatalysisSpring 2015
CHEM B486 Lecture – Gene Expression and PhysiologySpring 2012
CHEM C582/BIOC B505 Lecture – Biomolecular CatalysisSpring 2012
CHEM C117 Discussion – Principles of Chemistry and Biochemistry ISpring 2011
CHEM C103 Discussion – Introduction to Chemical PrinciplesFall 2010
University of Illinois at Urbana-Champaign
Advanced Analytical Chemistry Laboratory and LectureFall 2004
Accelerated Chemistry Lab IISpring 2004
Accelerated Chemistry Lab IFall 2003
Rose-Hulman Institute of Technology
Laboratory – General Chemistry IIIWinter 2002-2003
Laboratory – Organic Chemistry IIISpring 2003
Lecture – Engineering Statistics IIFall 2002
Service:
2013 Gordon Research Conference on Biological Molecules in the Gas Phase & in Solution
Assistant to the chair
246th American Chemical Society National Meeting and ExpositionFall 2013
ANYL symposium organizer, New Techniques and Methods in Proteomics – cancelled
Clemmer lab webmaster2011 –Present
Peer Review Assignments:
Analytical Chemistry2014 – Present
Outreach:
Foundations in Science and Mathematics, Chemistry InstructorSummer 2013
Publications:
18. Ewing, M.A.; Glover, M.S.; Clemmer, D.E. Hybrid Ion Mobility and Mass Spectrometry as a Separation Tool. Submitted.
17. Jung, J.E.; Ewing, M.A.; Valentine, S.J.; Przybylski, M.; Clemmer, D.E. Structural Insights Into Linkage-Specific Ubiquitin Chains Using Ion Mobility-Mass Spectrometry. Submitted.
16. Shi, L.; Holliday, A.E.; Glover, M.S.; Ewing, M.A.; Russell, D.H.; Clemmer, D.E. A Balance of Energy and Entropy Across Multiple Intermediates Enables Solution-Induced Folding of Polyproline Helices. Submitted.
15. Larriba-Andaluz, C.; Fernandez-Garcia, J.; Ewing, M.A.; Hogan, C.; Clemmer, D.E. Gas Molecule Scattering & Ion Mobility Measurements for Organic Macro-ions in He versus N2 Environments. Phys. Chem. Chem. Phys.2015,17, 15019-15029.
14. Ewing, M.A.; Conant, C.R.P.; Zucker, S.M.; Griffith, K. J.; Clemmer, D.E. Selected Overtone Mobility Spectrometry. Anal. Chem.2015, 87, 5132-5138.
13. Shi, L.; Holliday, A.E; Shi, H.; Zhu, F.; Ewing, M.A.; Russell, D.H.; Clemmer, D.E. Characterizing Intermediates along the Transition from Polyproline I to Polyproline II using Ion Mobility Spectrometry-Mass Spectrometry, J. Am. Chem. Soc.2014,136, 12702-12711.
12. Zucker, S.M.; Ewing, M.A.; Clemmer, D.E. A Gridless Ion Elimination Mechanism for Attomole Detection with Overtone Mobility Spectrometry,Anal. Chem.2013, 85, 10174-10179.
11. Glaskin, R.S.; Ewing, M.A.; Clemmer, D.E. Ion Trapping for Improving Ion Mobility Spectrometry Measurements in a Cyclical Drift Tube, Anal. Chem.2013,85, 7003-7008. Editors Highlight.
10. Ewing, M.A.; Zucker, S.M.; Valentine, S.J.; Clemmer, D.E. Overtone Mobility Spectrometry: Part 5. Simulations and Analytical Expressions Describing Overtone Limits. J. Am. Soc. Mass Spectrom.2013, 24, 768-779.
9. Dilger, J.M.; Valentine, S.J.; Glover, M.S.; Ewing, M.A.; Clemmer, D.E. A Database of Alkali Metal-Containing Peptide Cross-Sections: Influence of Metals on Size Parameters for Specific Amino Acids. Int. J. Mass Spectrom.2012,330-332, 35-45.
8. Valentine, S.J.; Ewing, M.A.; Dilger, J.M.; Glover, M.S.; Geromanos, S.J.; Hughes, C.; Clemmer, D.E. Using Ion Mobility Data to Improve Peptide Identification: Intrinsic Amino Acid Size Parameters. J. Proteome Res.2011,10, 2318-2329.
7. Lee, S.; Ewing, M.A.; Nachtigall, F.M.; Kurulugama, R.T.; Valentine, S.J.; Clemmer, D.E.. Determination of cross sections by overtone mobility spectrometry: evidence for loss of unstable structures at higher overtones. J. Phys. Chem. B2010,114, 12406-12415.
6. Ewing, M.A.; Wang, J.; Sheeley, S.A.; Sweedler, J.V.. Detecting D-amino acid-containing peptides using selective enzymatic digestion. Anal. Chem.2008,80, 2874-2880.
5. Hummon, A.B.; Richmond, T.A.; Verleyen, P.; Baggerman, G.; Huybrechts, J.; Ewing, M.A.; Vierstraete, E.; Rodriguez-Zas, S.L.; Schoofs, L.; Robinson, G.E.; Sweedler, J.V. From the genome to the proteome: uncovering peptides in the Apis brain. Science2006,314, 647-649.
4. Honeybee Genome Sequencing Consortium. Insights into social insects from the genome of the honeybee Apis melifera. Nature2006,443, 931-949.
3. Sheeley, S.A.; Miao, H.; Ewing, M.A.; Rubakhin, S.S.; Sweedler, J.V. Measuring D-amino acid-containing neuropeptides with capillary electrophoresis. Analyst2005,130, 1198-1203.
2. Myung, S.; Lee, Y.J.; Moon, M.H.; Taraszka, J.; Sowell, R.; Koeniger, S.; Hilderbrand, A. E.; Valentine, S. J.; Cherbas, L.; Cherbas, P.; Thomas, C.; Miller, D. F.; Mechref, Y.; Novotny, M. V.; Ewing, M. A.; Sporleder, C. R.; Clemmer, D. E. Development of high-sensitivity ion trap ion mobility spectrometry time-of-flight techniques: A high-throughput nano-LC-IMS-TOF separation of peptides arising from a Drosophila protein extract. Anal. Chem.2003,75, 5137-5145.
1. Meyerhoff, J.B.; Ewing, M.A.; Ewing, A.G. Ultrasmall enzyme electrodes with response times less than 100 milliseconds. Electroanalysis1999, 11, 308-312.
Patents:
Clemmer, D.E.; Ewing, M.A. Hybrid ion mobility spectrometer e.g. single-pass ion mobility spectrometer, for investigating properties of e.g. ions, has set of ion gates controlled between open and closed positions to selectively pass ions into multiple-pass drift tube. WO2015048014-A1.
Clemmer, D.E.; Ewing, M.A.; Zucker, S.M.; Conant, C.R.P. Ion Mobility Spectrometer and Method of Operating Same. US8872102-B2, US 20130292562 A1, US20150041637 A1.
Invited Talks:
Ewing, M.A.; Zucker, S.M.; Glover, M.S.; Clemmer. D.E. “Zoom Mode Ion Mobility Spectrometry.” 65th Pittsburgh Conference for Analytical Chemistry and Applied Spectroscopy, Chicago, IL, March 3, 2014.
Ewing, M.A.; Zucker, S.M.; Glover, M.S.; Clemmer. D.E. “Towards Zoom Mode Ion Mobility Spectrometry.” Eastman Chemical Company, Kingsport, TN, October 21, 2013.
Oral Presentations:
Shi, L; Holliday, A.; Glover, M.; Ewing, M.; Russell, D.; Clemmer, D. “Ion Mobility-Mass Spectrometry Reveals the Energy Landscape of Polyproline Folding.”63rd ASMS Conference on Mass Spectrometry and Allied Topics, St. Louis, MO, June 3, 2015.
Clemmer, D.E.; Shi, L.; Pierson, N.; Shi, H.; Ewing, M. “Developing IMS-MS techniques as a means of following structural transitions of biopolymers in solution.” 246th American Chemical Society National Meeting and Exposition, Indianapolis, IN, September 11, 2013.
Ewing, M.A.; Griffith, K.J.; Zucker, S.M.; Conant, C.R.P.; Clemmer, D.E. “Overtone mobility spectrometry: Selection of overtones.” 246th American Chemical Society National Meeting and Exposition, Indianapolis, IN, September 12, 2013.
Ewing, M.A.; Wang, J.; Sweedler, J.V. “Using mAAP to Search for D-Amino Acid-Containing Peptides in the Aplysia californica Brain.” 57th Pittsburgh Conference for Analytical Chemistry and Applied Spectroscopy, Orlando, FL, March 12 – 17, 2006.
Ewing, M.A.; Wang, J.; Sweedler, J.V. “A new method for finding D-amino acid-containing Peptides.” Turkey Run 2005 Analytical Chemistry Conference, Marshall, IN, November 5, 2005.
Sweedler, J.; Miao, H.; Ewing, M.; Scanlan, C.; Rubakhin, S. “Chirality in your Brain: Detecting D-Amino Acids and D-Amino Acid-Containing Peptides in Neurons.” ACS National Meeting, Washington, DC, August 2005.
Ewing, M.A.; Wang, J. Sweedler, J.V. “Detecting D-Amino Acid-Containing Peptides.” 56th Pittsburgh Conference for Analytical Chemistry and Applied Spectroscopy, Orlando, FL, Feb 27 – March 4, 2005.
Ewing, M.A.; Wang, J.; Sweedler, J.V. “Detecting D-amino acid-containing neuropeptides in the CNS of Aplysia californica.” 36th ACS Great Lakes Regional Meeting, Peoria, IL, Oct 18, 2004.
Ewing, M.A.; Sheeley, S.A.; Sweedler, J.V. “Detecting D-Amino Acid-Containing Peptides.” 55th Pittsburgh Conference for Analytical Chemistry and Applied Spectroscopy, Chicago, IL, March 8 – 12, 2004.
Ewing, M.; Jelski, D. “A Model for Displacive Anharmonic Vibrational Motions.” Butler University 14th Annual Undergraduate Research Conference, Indianapolis, IN, April 12, 2002.
Ewing, M.; Nei, P.; Schneider, T. “Rookies in the Mathematical Modeling Contest.” 17th Annual Rose-Hulman Undergraduate Math Conference, Terre Haute, IN, March 18, 2000.
Poster Presentations:
Ewing, M.; Glover, M.; Zucker, S.; Clemmer, D. “Zoom Mode Ion Mobility Spectrometry.” 63rd ASMS Conference on Mass Spectrometry and Allied Topics, St. Louis, MO, June 1, 2015.
Ewing, M. A.; Zucker, S. M.; Glover, M. S.; Clemmer, D. E. “Zoom Mode Ion Mobility Spectrometry.” NOBCChE Research Symposium, Bloomington, IN, September 27, 2014.
Zucker, S.M.; Ewing, M.A.; Clemmer, D.E. “Gridless Overtone Mobility Spectrometry.” Biological Molecules in the Gas Phase & in Solution Gordon Research Conference, Holderness, NH, July 24, 2013.
Ewing, M.A.; Conant, C.R.P.; Zucker, S.M.; Griffith, K.J.; Clemmer, D.E. “Overtone Mobility Spectrometry for Examining Structural Transitions in the Gas-Phase.” Biological Molecules in the Gas Phase & in Solution Gordon Research Conference, Holderness, NH, July 22, 2013.
Ewing, M.A.; Zucker, S.M.; Valentine, S.J.; Clemmer, D.E.. “Insights into Overtone Mobility Spectrometry.” Turkey Run 2012 Analytical Chemistry Conference, Marshall, IN, Nov 2, 2012.
Conant, C.R.P.; Ewing, M.A.; Clemmer, D.E. “Selected Overtone Mobility Spectrometry: Expanding the Range of Overtone Mobility Spectrometry.” 2012 Turkey Run Analytical Chemistry Conference, Marshall, IN, Nov 2, 2012
Zucker, S.M.; Ewing, M.A.; Glaskin, R.S.; Valentine, S.J.; Clemmer, D.E. “Gridless Overtone Mobility Spectrometry with Continuous RF Confinement.” Turkey Run 2012 Analytical Chemistry Conference, Marshall, IN, Nov 2, 2012
Ewing, M.A.; Valentine, S.J.; Dilger, J.M.; Glover, M.S.; Geromanos, S.; Hughes, C.; Clemmer, D.E. “The Use of a Synapt-Generated Cross Section Database and Intrinsic Amino Acid Size Parameters for the Improvement of Peptide Identification.” 59th Annual ASMS Conference on Mass Spectrometry and Allied Topics, Denver, CO, June 7, 2011.
Ewing, M.A.; Wang, J.; Sweedler, J.V. “Detecting D-Amino Acid-Containing Neuropeptides in the CNS of Aplysia californica.” 53rd ASMS Conference on Mass Spectrometry, San Antonio, TX, June 5 – 9, 2005.
Ewing, M.A.; Jordan, K. D. “A Model Potential for Water.” Duquesne 2001 Summer Research Symposium, Pittsburgh, PA, July 9, 2001