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Table S1Biochemical properties of 33 antimicrobial Streptomyces strains isolated from Tawang, India. ‘+’ positive for the test; ‘-’ negative for the test.

Table S2Phylogenetic affiliation of the antimicrobial Streptomyces strains as recorded after NCBI-BLAST search.

Fig.S3Antifungal radial growth inhibition assay of Streptomyces strains TSR23, TSR22, TSR18, TSR24, TSR12 and TSR28 against F.oxysporum f. sp. ciceri. Exponential culture filtrates were either (a) treated with or (b) without allosamidin for comparative halo forming ability. Complete to partial decline in activity is observed with chitinase family 18 inhibitor treated extract. Zone diameter in mm was recorded. Similar results were obtained against test phytopathogen of F. moniliforme and R.solani (data not shown).

Table S1

Streptomyces strain / Melanoid pigment / L- Arabinose / Sucrose / D-Xylose / D-Fructose / D-Mannitol / Raffinose / L- Rhamnose / Caseine / Tyrosine / Urea
Streptomyceskanamyceticus TSR1
Creamy whitish and reverse is yellow brown / + / + / + / + / + / - / - / - / + / - / +
Streptomycescirratus TSR2 (spore mass is grey colour) / - / - / + / - / - / - / - / - / + / - / +
Streptomycesindigoferus TSR3 (spore mass is grey) / + / + / - / - / - / - / - / - / + / - / -
Streptomycesxanthocidicus TSR4 (spore mass is white to grey, spore chains are rectiflexibiles) / - / - / - / - / - / + / - / - / + / + / +
Streptomycespurpeofuscus TSR6 (spore mass is grey-black and reverse is greyish yellow) / + / - / - / - / - / + / - / - / + / - / +
Streptomycessporoverrucosus TSR7
The spore chains were in the form of terminal, closed, or compact spirals. The mature aerial; Spore mass colours is light green colour. The reverse side of the colonies were faint yellow. / - / + / - / - / - / - / - / - / + / + / +
Streptomycessporoverrucosus TSR9
Spore mass colours are light green colour. The reverse sides of the colonies were faint yellow. / - / + / + / - / - / - / + / - / + / + / +
Streptomycespurpeofuscus TSR11
(Spore mass is grey-black and reverse is greyish yellow) / + / - / + / - / - / - / + / - / + / - / -
Streptomyces roseochromogenus TSR12 / + / - / - / - / - / - / + / - / + / - / -
Streptomycescirratus TSR13
Spores mass is grey colour) / + / - / - / - / - / - / - / - / + / - / +
StreptomycesvirginiaeTSR16
(Spore mass is white abundant grey; pink pigment produced) / + / - / - / + / + / - / - / - / + / - / -
Streptomycesspororaveus TSR18
Spore mass is white, reverse is yellow brown. / - / - / - / + / - / - / - / - / + / - / -
Streptomycesaureus TSR19
Spore mass is white and brick red colour / + / - / - / + / - / + / - / + / + / + / -
StreptomycescirratusTSR20
(Spores mass is grey colour) / - / - / - / - / - / + / - / + / + / + / +
Streptomyceschartreusis TSR21
Spore mass is grey, reverse is yellow brown, Spore chains are spiral. / - / + / - / - / - / + / - / + / + / + / +
Streptomycesbadius TSR22
Spore mass is white olive green, reverse is yellow. / + / + / - / - / - / + / - / + / + / + / +
Streptomycesgriseoplanus TSR23
Spore mass is light yellow. / - / + / - / - / - / + / - / - / + / - / +
Streptomyces roseochromogenus TSR24
Spore mass is pink in initial stage and later turned to grey colour. / - / - / - / - / + / - / + / - / + / - / +
Streptomycesaureus TSR25
Spore mass is white and brick red colour / - / - / - / + / - / - / + / - / + / - / -
Streptomycessanglieri TSR27
Spore mass is black grey / + / - / - / - / - / - / + / - / + / - / +
StreptomycesspororaveusTSR28
Spore mass is white, reverse is yellow / - / - / - / - / - / - / + / + / + / - / +
StreptomycessanglieriTSR29
Spore mass is black grey / + / + / - / - / - / - / - / - / + / - / -
StreptomycessanglieriTSR30
Extensively branched. Spore mass is black grey. / + / - / + / - / - / - / - / - / + / - / -
Streptomycescirratus TSR36
(Spores mass is grey colour) / - / - / - / - / - / - / - / + / + / - / +
Streptomycesvirginiae TSR37
(Spore mass is white abundant grey; Pink pigment produced) / + / - / - / + / + / - / - / + / + / + / +
Streptomycesgoshikiensis TSR38
Spore mass is pink to grey / - / + / - / - / - / + / - / + / + / + / +
Streptomycesvinaceus TSR39
Spore mass is brick red / - / + / + / - / - / + / - / - / + / + / +
StreptomycesrishiriensisTSR41
Spore mass is pale grey colour / - / + / - / - / - / - / - / - / + / + / +
Streptomyceshumidus TSR43
(Spore mass is whitish colour) / - / - / - / - / - / - / - / - / + / + / -
Streptomycescirratus TSR45
(Spores mass is grey colour) / - / - / - / - / - / - / - / - / + / + / +
Streptomycespurpeofuscus TSR46
(Spore mass is light grey and reverse is greyish yellow) / + / - / - / - / - / - / - / - / + / + / -
Streptomycescirratus TSR48
(Spores mass is grey colour) / - / - / + / - / - / - / - / - / + / - / -
StreptomycesshaanxiansisTSR50
(Spores mass is grey colour) / + / - / + / - / - / - / - / - / + / - / +

Table S2

Organisms name and GenBank Accession no. / Length (bp) / Match region / Significant Blast hit match / Accession no. / Percentage identity
Streptomyceskanamyceticus TSR1 (JF707861) / 1345 / 76 – 1416 / Streptomycesaureus strain HBUM174596 / EU841581 / 98%
Streptomycescirratus TSR2 (JF338857) / 1392 / 40-1424 / Streptomycesvinaceus strain NBRC 13425 / AB222069 / 99%
Streptomycesindigoferus TSR3 (JF707862) / 1415 / 9-1419 / Streptomycesindigoferus strain 30-4 / EU054371 / 98%
Streptomycesxanthocidicus TSR4 (JF707863) / 1426 / 20-1440 / Streptomycestsukiyonensis / AB184594 / 98%
Streptomycespurpeofuscus TSR6 (JF707864) / 1390 / 22-1415 / Streptomycespurpeofuscus / AB184234 / 99%
Streptomycessporoverrucosus TSR7
(JF707865) / 1382 / 59-1423 / Streptomycessporoverrucosusstrain NRRL B-16379 / NR_043837 / 99%
Streptomycessporoverrucosus TSR9 (JF707866) / 1366 / 32-1390 / Streptomyceslavendulae strain HD-8 / JN609386 / 99%
Streptomycespurpeofuscus TSR11 (JF707867) / 1345 / 52-1385 / Streptomycespurpeofuscus / AB184234 / 98%
Streptomyces roseochromogenus TSR12
(JF707868) / 1379 / 46-1404 / Streptomyceslavendulae strain HD-8 / JN609386 / 99%
Streptomycescirratus TSR13 (JF707869) / 1413 / 35-1447 / Streptomycescirratus / AB184377 / 99%
StreptomycesvirginiaeTSR16 (JF707870) / 1372 / 46-1398 / Streptomycesvirginiae strain SZQNH15 / JN999924 / 99%
Streptomycesspororaveus TSR18 (JF755879) / 1402 / 47-1441 / Streptomycesspororaveus strain 173620 / EU570406 / 99%
Streptomycesaureus TSR19 (JF755880) / 1417 / 48-1437 / Streptomycesaureus strain HBUM174596 / EU841581 / 98%
StreptomycescirratusTSR20 (JF932302) / 1297 / 48-1345 / Streptomycescirratus / AB184377 / 99%
Streptomyceschartreusis TSR21 (JF755881) / 1368 / 18-1375 / Streptomycesnatalensis strain 20-4 / EU367983 / 99%
Streptomycesbadius TSR22 (JF755882) / 1339 / 30-1365 / Streptomycesbadius strain HBUM175013 / FJ486408 / 99%
Streptomycesgriseoplanus TSR23 (JF755883) / 1431 / 10-1390 / Streptomycessporovirgulis strain TGNBSA5 / JQ654447 / 99%
Streptomyces roseochromogenus TSR24 (JF755884) / 1384 / 32-1406 / Streptomycesgoshikiensis strain XZYM25 / HQ844511 / 99%
Streptomycesaureus TSR25 (JF755885) / 1375 / 25-1389 / Streptomycesaureus strain HBUM174596 / EU841581 / 99%
Streptomycessanglieri TSR27 (JF755886) / 1416 / 5-1421 / Streptomycessanglieri strain NBRC 100784 / NR_041417 / 99%
StreptomycesspororaveusTSR28 (JF755887) / 1342 / 41-1374 / Streptomycesspororaveus strain HBUM174519 / EU877063 / 99%
StreptomycessanglieriTSR29 (JF755888) / 1381 / 49-1423 / Streptomycesnigrogriseolus / AB184460 / 99%
StreptomycessanglieriTSR30 (JF825427) / 1398 / 36-1426 / Streptomycessanglieri strain NBRC 100784 / NR_041417 / 99%
Streptomycescirratus TSR36 (JF825428) / 1382 / 53-1431 / Streptomycesvinaceus strain NBRC 1342 / NR_041131 / 99%
Streptomycesvirginiae TSR37 (JF825429) / 1401 / 35-1417 / Streptomycesvirginiae strain ZJHG2 / JN999923 / 99%
Streptomycesgoshikiensis TSR38 (JF825430) / 1361 / 69-1410 / Streptomycesgoshikiensis strain HBUM173360 / EU841663 / 99%
Streptomycesvinaceus TSR39 (JF825431) / 1466 / 5-1465 / Streptomycesvinaceus strain NBRC 13425 / NR_041131 / 99%
StreptomycesrishiriensisTSR41 (JF825432) / 1402 / 15-1417 / Streptomycesrishiriensis strain NRRL B-3239 / NR_044141 / 99%
Streptomyceshumidus TSR43 (JF825433) / 1333 / 65-1398 / Streptomyceshumidus / AB184339 / 98%
Streptomycescirratus TSR45 (JF825434) / 1353 / 35-1387 / Streptomycescirratus / AB184377 / 99%
Streptomycespurpeofuscus TSR46 (JF825435) / 1384 / 25-1413 / Streptomycespurpeofuscus / AB184234 / 99%
Streptomycescirratus TSR48 (JF825436) / 1292 / 92-1379 / Streptomycesvinaceus strain NBRC 13425 / NR_041131 / 99%
StreptomycesshaanxiansisTSR50 (JF338142) / 1148 / 59-1206 / Streptomyces viridochromogenes
subsp. komabensis / AB184536.1 / 98%

Fig. S3