Supplemental Data. Chen Et Al. Plant Cell. (2012). 10.1105/Tpc.112.095984

Supplemental Data. Chen et al. Plant Cell. (2012). 10.1105/tpc.112.095984

Supplemental Data Set 1. Multiple Sequence Alignment of SIP2- Homologs

AtMEK4 MRPIQSPPG-VSVPV------KSRPRRRPDLTLPLPQRDVSLAVPLPLPPTSGGSGGSSGSA 55

AtMEK5 MKPIQSPPGGVASPT------KNRSRKRPDLSLPLPHRDVALAVPLPLPPPSSSSSAPASSS 56

LjSIP2 MRPIQLPPPSTSATGSPGAAANNNINRDQRPQRRRKDHLTLPLPQRDTNLAVPLPLPPSGGSGGSGGSGG 70

MsSIMKK MRPIQLPPP--TATGSATASGSPNNNNNSRPQRRRKDHLTLPLPQRDTNLAVPLPLPPSGGSGGSGGGGN 68

AtMEK7 MALV------RKRRQINLRLPVPP------LSVHLP--WFSFASSTAPV 35

AtMEK9 MALV------RERRQLNLRLPLPP------ISDR----RFSTSSSSATT 33

AtMEK8 MVRV------RDHRFLNLKLSPIQ------APTTIPPCRLPIVAATKAS 37

AtMEK10 MTLV------RERRHQEPLTLSIP------PLLYHRTAFSVASSSS-- 34

LjMKK10 MTLVI------RERRHKQALTLSLP------KPKIPPSDFPYQTHTPPL 37

AtMEK2 MKKGGFSNN-LK------LAIPVAGEQSITKFLRKC-FCSLCRTQSG-----TFKDGDLRVNKDGVRI 55

LjMKK2 MNRGNFGLN-LK------LSVPAPNEATYAKFL------TQSG-----TFKDGDLLVNRDGVRI 46

AtMEK1 MNRGSLCPN--P------ICLP-PLEQSISKFL------TQSG-----TFKDGDLRVNKDGIQT 44

AtMEK6 MVKIKSNLKQLK------LSVP-AQESPISSFL------TASG-----TFHDGDFLLNQKGLRL 46

AtMEK3 MAALEELKKKLSPLFDAEKGFSSSSSLDPNDSYLLSDGGTVNLLSRSYG-----VYNFNELGLQKCTSSH 65

AtMEK4 PSSGGSASSTNTNSSIEAKNYSDLVRGNRIGSGAGGTVYKVIHRPSSRLYALKVIYGNHEETVRRQICRE 125

AtMEK5 AISTNISA------AKSLSELERVNRIGSGAGGTVYKVIHTPTSRPFALKVIYGNHEDTVRRQICRE 117

LjSIP2 SGGGGGLSQLQQQLVI---PFSELERLNRIGSGSGGTVYKVVHRTTGRVYALKVIYGHHEESVRRQIHRE 137

MsSIMKK GSGSGGAS---QQLVI---PFSELERLNRIGSGSGGTVYKVVHKINGRAYALKVIYGHHEESVRRQIHRE 132

AtMEK7 IN------N------GISASDVEKLHVLGRGSSGIVYKVHHKTTGEIYALKSVNGDMSPAFTRQLARE 91

AtMEK9 TTVAGC--N------GISACDLEKLNVLGCGNGGIVYKVRHKTTSEIYALKTVNGDMDPIFTRQLMRE 93

AtMEK8 ATVSSCASN------SFSVANLDRINVLGSGNGGTVFKVKDKTTSEIYALKKVKENMDSTSP---CRE 96

AtMEK10 -SSPDSSPVET------INDLEKLAVLGQGSGGTVYKTRHRRTKTLYALKVLRSNLNTTTTTTTAVE 94

LjMKK10 FSSPSSHLINSCSYI---RNISDLEKVAVLGHGYGGTVYKVHHKENHSFYALKVRRFK------TLE 95

AtMEK2 ISQSEPEVLSPIKPADDQLSLSDLDMVKVIGKGSSGVVQLVQHKWTGQFFALKVIQLNIDEAIRKAIAQE 125

LjMKK2 VSQSEVEAPPPIKAVDNQLSLADIDVIKVVGKGSGGVVQLVQHKWTSQFFALKIIQMNIEESIRKQIAQE 116

AtMEK1 VSLSEPGAPPPIEPLDNQLSLADLEVIKVIGKGSSGNVQLVKHKLTQQFFALKVIQLNTEESTCRAISQE 114

AtMEK6 MSDEKQSRQSDSKELDFEITAEDLETVKVIGKGSGGVVQLVRHKWVGKFFAMKVIQMNIQEEIRKQIVQE 116

AtMEK3 VDESESS------ETTYQCASHEMRVFGAIGSGASSVVQRAIHIPNHRILALKKINI-FEREKRQQLLTE 128

AtMEK4 IEI-LRDVNHPNVVKC-HEMF------DQN--GEIQVLLEFMDKGSLEGA---H-VWKEQQLADLSRQ 179

AtMEK5 IEI-LRSVDHPNVVKC-HEMF------DHN--GEIQVLLEFMDQGSLEGA---H-VWQEQELADLSRQ 171

LjSIP2 IQI-LRDVDDVNVVKC-HEMY------DHN--AEIQVLLEFMDGGSLEGK---H-IPQEQQLADVARQ 191

MsSIMKK IQI-LRDVDDVNVVKC-HEMY------DHN--AEIQVLLEYMDGGSLEGK---H-IPQENQLADVARQ 186

AtMEK7 MEI-LRRTDSPYVVRC-QGIF------EKPIVGEVSILMEYMDGGNLESL---RGAVTEKQLAGFSRQ 148

AtMEK9 MEI-LRRTDSPYVVKC-HGIF------EKPVVGEVSILMEYMDGGTLESL---RGGVTEQKLAGFAKQ 150

AtMEK8 IEI-LRVVNSPYVVKC-HDIF------QNPS-GEVSILMEYMDLGTLESL---SG-VSENQLALMARQ 151

AtMEK10 VDI-LKRIKSSFIVKC-YAVFLNSS------DLCFVMELMEKGSLHDALLAQQVFTEPMISTLANR 152

LjMKK10 AEI-LKRVDSPYIVKC-HAVFDNGACSESDNNGGGDLSFVMEYMERGSLDDVLREHHRLPEEVISVMANR 163

AtMEK2 LKINQ-SSQCPNLVTSYQS-FY------DNGAISLILEYMDGGSLADFLKSVKTIPDSYLSAIFRQ 183

LjMKK2 LKINQ-SAQCPYLVVCYQS-FY------DNGVISIILEYMDGGSLADLLKKVKTIPEPYLAAICKQ 174

AtMEK1 LRINL-SSQCPYLVSCYQS-FY------HNGLVSIILEFMDGGSLADLLKKVGKVPENMLSAICKR 172

AtMEK6 LKINQASSQCPHVVVCYHS-FY------HNGAFSLVLEYMDRGSLADVIRQVKTILEPYLAVVCKQ 175

AtMEK3 IRT-LCEAPCHEGLVDFHGAFYS------PDSGQISIALEYMNGGSLADILKVTKKIPEPVLSSLFHK 189

AtMEK4 ILSGLAYLHS-RHIVHRDIKPSNLLINSAKNVKIADFGVSRILAQTMDPCNSSVGTIAYMSPERINTDLN 248

AtMEK5 ILSGLAYLHR-RHIVHRDIKPSNLLINSAKNVKIADFGVSRILAQTMDPCNSSVGTIAYMSPERINTDLN 240

LjSIP2 ILRGLAYLHR-RHIVHRDIKPSNLLINSRKQVKIADFGVGRILNQTMDPCNSSVGTIAYMSPERINTDIN 260

MsSIMKK ILRGLAYLHR-RHIVHRDIKPSNLLINSRKQVKIADFGVGRILNQTMDPCNSSVGTIAYMSPERINTDIN 255

AtMEK7 ILKGLSYLHS-LKIVHRDIKPANLLLNSRNEVKIADFGVSKIITRSLDYCNSYVGTCAYMSPERFDS-AA 216

AtMEK9 ILKGLSYLHA-LKIVHRDIKPANLLLNSKNEVKIADFGVSKILVRSLDSCNSYVGTCAYMSPERFDSESS 219

AtMEK8 VLEGLNYLHE-HKIVHRDIKPANLLRSSKEEVKITDFGVSKIVVKSFNKCNSFVGTYAYMSPERLDSEAD 220

AtMEK10 ILQGLRYLQE-MRIVHGDIKPSNLLINNKGEVKIADFGASRIVAGGDY---GSNGTCAYMSPERVDPEKW 218

LjMKK10 VLEGLKYLHG-MKIVHRDIKPSNLLVNDKGEVKIADFGVSHVVEEEAD---SNDGTYAYMSPERFDLSRW 229

AtMEK2 VLQGLIYLHHDRHIIHRDLKPSNLLINHRGEVKITDFGVSTVMTNTAGLANTFVGTYNYMSPERIIGN-- 251

LjMKK2 VLKGLMYLHHERHIIHRDLKPSNLLINHIGEVKITDFGVSTIMESTSGQANTFIGTYNYMSPERINGS-- 242

AtMEK1 VLRGLCYIHHERRIIHRDLKPSNLLINHRGEVKITDFGVSKILTSTSSLANSFVGTYPYMSPERISGS-- 240

AtMEK6 VLLGLVYLHNERHVIHRDIKPSNLLVNHKGEVKISDFGVSASLASSMGQRDTFVGTYNYMSPERISGS-- 243

AtMEK3 LLQGLSYLHGVRHLVHRDIKPANLLINLKGEPKITDFGISAGLENSMAMCATFVGTVTYMSPERI----- 254

AtMEK4 ----QGKYDGYAGDIWSLGVSILEFYLGRFPF-PVSRQGDWAS---LMCAICMSQPPEAPATA-SPEFRH 309

AtMEK5 ----HGRYDGYAGDVWSLGVSILEFYLGRFPF-NVSRQGDWAS---LMCAICMSQPPEAPVTA-SQEFRH 301

LjSIP2 ----DGQYDAYAGDIWSFGVSILEFYMGRFPF-AVGRQGDWAS---LMCAICMSQPPEAPPTA-SLEFRD 321

MsSIMKK ----DGQYDAYAGDIWSLGVSILEFYMGRFPF-AVGRQGDWAS---LMCAICMSQPPEAPTTA-SPEFRD 316

AtMEK7 G----ENSDVYAGDIWSFGVMILELFVGHFPLLPQGQRPDWAT---LMCVVCFGEPPRAPEGC-SDEFRS 278

AtMEK9 G----GSSDIYAGDIWSFGLMMLELLVGHFPLLPPGQRPDWAT---LMCAVCFGEPPRAPEGC-SEEFRS 281

AtMEK8 GVTEEDKSNVYAGDIWSFGLTMLEILVGYYPML 253

AtMEK10 GF---GGEVGFAGDVWSLGVVVLECYIGRYPLTKVGDKPDWAA---LICAICCNEKVEIPVSG-SPEFRD 281

LjMKK10 GG---ENVDEFAGDVWSLGVVMLECFLGHFPFIDPGQKPDLVT---LVCAICFGEKLEMPEEA-SPEFQS 292

AtMEK2 ----K---YSNKSDIWSLGLVVLECATGKFPYAPPNQEETWTSVFELMEAIVDQTPPALPSGNFSPELSS 314

LjMKK2 ----HEHGYNYKSDIWSLGLILLECAMGRFPYAPPDQSETWESIFELIETVVDKPPPSAPSEQFSTEFCS 308

AtMEK1 ----L---YSNKSDIWSLGLVLLECATGKFPYTPPEHKKGWSSVYELVDAIVENPPPCAPSNLFSPEFCS 303

AtMEK6 ------TYDYSSDIWSLGMSVLECAIGRFPYLESEDQQNPPSFYELLAAIVENPPPTAPSDQFSPEFCS 306

AtMEK3 ----RNDSYSYPADIWSLGLALFECGTGEFPYIANEGP------VNLMLQILDDPSPTPPKQEFSPEFCS 314

AtMEK4 FISCCLQREPGKRRSAMQLLQHPFILRASPSQNRSPQNLHQLLPPP-R------356

AtMEK5 FVSCCLQSDPPKRWSAQQLLQHPFILKAS-----GGPNLRQMLPPP-R------343

LjSIP2 FIGRCLQRDPSRRLSASRLLAHPFLAQSSPHNNQPPPNLHQLLPPPPR------369

MsSIMKK FVSRCLQRDPSRRWTASRLLSHPFLVRNGSNHNQSPPNMHQLLPPPPR------364

AtMEK7 FVDCCLRKESSERWTASQLLGHPF------LRESL 307

AtMEK9 FVECCLRKDSSKRWTAPQLLAHPF------LREDL 310

AtMEK8 253

AtMEK10 FVGRCLEKDWRKRGTVEELLRHSFVKNR 309

LjMKK10 FVKRCLEKDWRKRATVLELLHHPFVNRNCCCSSRG---LEDCV------332

AtMEK2 FISTCLQKDPNSRSSAKELMEHPFLNKYDYSGINLASY------352

LjMKK2 FISACLQKDPKDRLSAQELMRLPFTSMYDDLDVDLSAY------346

AtMEK1 FISQCVQKDPRDRKSAKELLEHKFVKMFEDSDTNLSAY------341

AtMEK6 FVSACIQKDPPARASSLELLSHPFIKKFEDKDIDL------341

AtMEK3 FIDACLQKDPDARPTADQLLSHPFITKHEKERVDLATFVQSIFDPTQRLKDLADMLTIHYYSLFDGFDDL 384

AtMEK4 ------PLSSSSSPTT 366

AtMEK5 ------PLPSAS 349

LjSIP2 ------SLSS 373

MsSIMKK ------SLSS 368

AtMEK7 307

AtMEK9 310

AtMEK8 253

AtMEK10 309

LjMKK10 ------LCG 335

AtMEK2 ------FTDAGSPLATL------363

LjMKK2 ------FSNAGSPLATL 357

AtMEK1 ------FTDAGSLIPPL------352

AtMEK6 ------GILVGTL------348

AtMEK3 WHHAKSLYTETSVFSFSGKHNTGSTEIFSALSDIRNTLTGDLPSEKLVHVVEKLHCKPCGSGGVIIRAVG 454

AtMEK4 366

AtMEK5 349

LjSIP2 373

MsSIMKK 368

AtMEK7 307

AtMEK9 310

AtMEK8 253

AtMEK10 309

LjMKK10 335

AtMEK2 ------GNLSGTF------SV 372

LjMKK2 357

AtMEK1 ------AN 354

AtMEK6 ------EP-PVNY------LR 356

AtMEK3 SFIVGNQFLICGDGVQAEGLPSFKDLGFDVASRRVGRFQEQFVVESGDLIGKYFLAKQELYITNLD 520