CURRICULUM VITAE
DARBHA NITHYANANDA SASTRY
620,Vinaya, Second Cross, Near garden,
Sector 5,Srinagar, Belgaum-590016
E-mail:
Mobile: 0 93422 50794
Objective: To pursue a challenging teaching and research career relevant to pharmaceutical sciences, and biotechnology.
Skills:
· Teaching biotechnology, bioprocess technology, pharmacy, microbiology, molecular pharmacology and related subjects, career guidance and motivation to students and faculty. Delivering lectures as resource person in quality improvement programs, seminars, journal clubs and workshops.
· Generation of new research ideas, projects relating to pharmaceutical, biotechnology and interdisciplinary research, literature and patent search, submission of project proposals.
Employment History
September 2005 –Till date:
Assistant Professor, Department of Pharmaceutical Biotechnology, K.L.E University’s College of Pharmacy, Belgaum.
My job involves
· Teaching principles of applied biochemistry, microbiology, pharmaceutical statistics for undergraduate programs, pharmaceutical biotechnology, molecular pharmacology, bioprocess technology for masters programs.
· Research relevant to pharmaceutical biotechnology with emphasis on screening of natural substrates for enzymes and bioactive compounds.
· Participating as resource person/delegate in quality improvement programs, seminars, journal clubs and workshops
Ongoing projects:
Isolation and Charecterization of Exopolysaccharide From Lactic acid bacteria and its Application in Formulation Development (M.Pharm Dissertation Project)
Use of Exopolysaccharide Isolated from Selected Microorganisms as Formulation Excipient. (M.Pharm Disertation Project)
Screening, Isolation and fermentative production of microbial pigments from fungi,bacteria and actinomycetes and their applications.
B.Tech biotechnology projects relevant to screening of alkalophiles, bioflocculant, biomineralization, biodegradation of PET plastic, biobutanol as co guide.
Sponsored research project guided: “Production of biocolours from endophytes of natural dye yielding plants & their applications “sponsored by Vision group of Science and Technology,Government of Karnataka Sponsored under (SPICE - 2011-12)
M.Pharm Projects Guided.
Name of the Project / Name of the M.Pharm StudentStudies on isolation and charecterization of the bacterial siderophore for antimicrobial activity / Mr. Bagde Parag Vijay Rao
Screening and charecterization of fungal siderophores for antimicrobial activity / Mr. Phadke Milind kashinath
Isolation and biological activities of secondary metabolites of endophytic fungi from Gymnema sylvestre / Miss. Tikole Sucheta Shivaji
Studies on production downstream processing and hypoglycemic effect of exopolysacccharides from isolated strains of Lactobacilli / Mr. Nikhil Kishore Potdukhe
Screening isolation and fermentative production of carotenoids from isolated microbial strains / Mr. Sambhasiva Rao Kolla
August 2004 - May 2005
Research Scientist, Reametrix India Pvt.Ltd, Bangalore. I was associated with a project relating to bioconjugations, expression & purification of immune system molecules of commercial and research interest in E.coli based expression system.
My job role involved
· Codon optimization, total gene synthesis, cloning of few optimized gene sequences.
· Refolding & purification of proteins using combination of chromatographic methods.
· Bioconjugation of few molecules, separation of bioactive conjugates and recovery of free protein using HPLC.
July 2002 - April 2004
Research assistant at Department of Biochemistry & Biophysics, Texas A&M University, Texas U.S.A. My project dealt with structural investigations of Chemokines.
My Job involved
· Bioinformatics of chemokine sequences, random, rational mutagenesis and selections
· Cloning, protein expression and purification of native and IL8, MIP1 beta mutants.
· General maintenance of lab supplies and instruments.
September 2001-April 2002
Guest Student Researcher at Department of Pharmaceutical biosciences, Biomedical Center, Uppsala University, Sweden. My responsibility was to conduct research relating to proteo-chemometrics and pharmaceutical pharmacology.
My job involved
· Literature search on G Protein coupled receptors, antigen & antibody sequences.
· Basic molecular biology techniques for construction of chimeric receptors.
· Transfections and membrane preparations.
July 1999 -June 2001
Project associate, Product Development Cell, National Institute of Immunology, New Delhi, India. Project title: 'Large Scale Insect cell Culture for the production of bioinsecticides and recombinant proteins using baculovirus'.
My job involved
· Maintenance of cell cultures and Cultivation of insect cells.
· Standardization and optimization of baculovirus-insect cell infection process.
· Purification of recombinant proteins from inclusion bodies of E.coli.
August 1998-July 1999:
Consultant for research and technical affairs, Yegna Envirochem Pvt. Ltd., Hyderabad, India. Responsibilities: Literature search, Guidance for research to develop new products and processes and commercialization of products and processes.
June1994-June1995:
Trainee in the Quality Assurance Department of Parke-Davis (India) Ltd. Uppal road, Hyderabad, India. Responsibilities: Analysis of Raw material, bulk in process, finished products and R&D sample by chemical and instrumental methods.
August 1992-May1994:
Product Executive in Cosmos Global Enterprises, Hyderabad, India. Responsibilities: Product identification, product Techno/commercial information, market researches and survey.
Educational Qualifications:
Enrolled for PhD program in Biotechnology, at JNTU, Hyderabad. Cleared pre-PhD exam with
General and industrial microbiology and bioprocess engineering& technology as electives.
PhD Topic: Isolation, fermentative production and characterization of microbial pigments
Aug1996- Aug1998
M.Pharm, with distinction from Department of Pharmaceutical Sciences, Andhra University, Vishakapatnam, Andhra Pradesh, India. Major: Pharmaceutical Biotechnology.
Project: 'Studies on the production parameters of an antibiotic with lytic properties from a streptomycete' with emphasis on screening of actinomycetes for secondary metabolites.
Aug1988- Aug1992
B.Pharm, from University college of Pharmaceutical sciences, Kakatiya University, Warangal, India. Major: Pharmaceutical Sciences.
Aug1995-Aug1996
OYPC Course in statistical methods and applications, cleared with first class from Hyderabad chapter of Indian Statistical Institute.
Publications:
Title: Investigation of CC and CXC chemokine quaternary state mutants.
Jin H, Hayes GL, Nithyananda S Darbha, Meyer E, Liwang PJ.
Biochem Biophys Res Commun. 2005 Dec 16;338(2):987-99. Epub 2005 Oct 21
Title: Evaluation of Plumbago indica callus for its plumbagin content and antimicrobial activity
Karadi R V, Kavatagimath S A, Gaviraj E N, Sastry D N, Chandrashekara S,
J Cell Tissue Res 2007, 7(2), 1131-6.
Paper Presentations:
Attended and presented many posters at various international and national conferences
Paper Title: ‘Isolation and characterization of a Carotenoid from a soil isolate’ at second international conference and Indo-Canadian satellite symposium on pharmaceutical sciences and technology, February 24-26, 2007, Ooty, India.
Paper Title: ‘Screening for microbial production of natural flavors and fragrances’ at
Indo-Australian conference on human variations and pharmacogenomics, March 17-19, 2007
Manipal, India.
Paper Title: ‘Fermentative performance of yeast strain for Xylitol production from D-Xylose’
at second international conference and Indo-Canadian satellite symposium on pharmaceutical sciences and technology, February 24-26, 2007, Ooty, India.
Paper Title: 'Culture of insect cells for production of baculoviruses, growth kinetics and metabolism' at a national symposium 'Emerging trends in biotechnology for the new millennium' February 26-27, 2000 Hyderabad, India.
Paper Title: 'Studies on production parameters of an antibiotic with lytic Properties' at Indian Pharmaceutical Congress December 18-21, 1998 Trivandrum, India.
Guest lectures delivered
Delivered lectures on selected topics of Medicinal Plant Biotechnology at Goa College of Pharmacy for M.Pharm Students.
Delivered Guest lecturers as resource person on
Challenges and Opportunities in Biotechnology product and process Development
Issues and Challenges in Protein Formulations
Trends, Opportunities & Challenges in Pharmacology & Toxicology research
Therapeutic antibodies:Trends, Challenges & Opportunities
Natural Dyes From Plants & Microbes
Endophytes from medicinal plants: A source of bioactive phytochemicals
Isothermal Titration Calorimetry (ITC) & Its Applications
Participated in Various other Quality Improvement Programs and workshops as a resource person and delivered lectures on
A) Statistical Process Control
B) Analytical techniques for biotechnological products
C) Receptor–ligand binding assays
D) Protein Kinase Inhibitors & Therapeutic targets
E) Molecular Approaches to Antiarrythmic drug research
G) Quality assurance of Vaccines: Technical and regulatory considerations
H) Quality assurance & control measures for biological and biotechnological products
I) An overview of Biopharmaceuticals
J) New approaches in vaccine development and delivery
L) Role of Drug Metabolism & Pharmacokinetics in Drug Research
M) Applications of Modern Analytical Equipments in pharmacological research
N) Molecular and cell based approaches for study of drug receptor interaction.
O) Design of experiments in pharmacological research.
P) Analysis of Dose-Response Data
Q) Phyto-pharmaceuticals Opportunities for drug discovery
R) Twentyone- Herbs of Ganesha festival.
S) Statistical methods and applications in Phamacy
T) Natural product drug discovery
U) Understanding Molecular pharmacology: An insight into receptor biology
V) Quality Assurance, Regulatory Affairs and Validation considerations for biotechnology derived products,
W) Estimation of biomarkers in saliva
X) Overview of molecular biology applications
Y) Molecular biology and drug discovery
Z) Statistical Control Charts
A1) Bio-Informatics, B1) Recombinant DNA techniques
Workshops/Conferences attended
DST sponsored national workshop on Taxonomy, EX-Situ Conservation and Bioprospecting of Fungi National Fungal Culture Collection Center of India(NFCCI) Agharkhar Research Institute, PUNE, from 25th May-8th June 2012.
AICTE sponsored Staff development programme on Managing Biotechnologies and Emerging Issues in Research and Development at Institute for Public Enterprise , Osmania University, Hyderabad from7th -14th February 2012.
Biochemistry CME-2011 on Nutrition in Health and Diseases held at KLE University, Belgaum on 27th August 2011
International Conference on Molecular Medicine "MOLMED-2012 Genes, Genomes and Chronic Disorders Vellore Institute of Technology,(VIT)Vellore, Tamilnadu from 19th to 22nd February 2012
Participated in“15th ADNAT Convention” a three day symposium on “Genomics and Biodiversity” from 23-25th February 2011 held at Centre for Cellular and Molecular Biology, Hyderabad, India
Participated in 15th Annual Conference of the Society of Pharmacognosy and First International Convention being held at KLE University’s College of Pharmacy, Belgaum from 18-20th February 2011
Participated in RMRC sponsored workshop on Molecular, Biological and Biotechnological advances in Herbal Medicine on 27th September 2010 organized by RMRC,ICMR, Belgaum
Participated as delegate in a workshop on “Principles of evaluation & question paper setting” held at KLE University, Belgaum on 6th October 2010
Participated as delegate in a workshop on “Teaching methodology and Education technology” held at KLE University, Belgaum from 10th – 11th June 2010
Participated in faculty development program of AICTE sponsored Biotechnology Teaching Excellence program (BTEP-10) held at School of Biotechnology, National Institute of Technology (NIT), Calicut from 24th – 29th May 2010
Participated as delegate in a CME on Clinical research by Indian Pharmacological society (IPS) 20th February, 2010 at KLE University Belgaum
Participated as delegate at 61st Indian Pharmaceutical Congress 2009 from 11th -13th December2009 at NIRMA University Ahmedabad.
Participated as scientific committee member for posters and delegate at 3rd Congress on Cosmetic Technology, held at KLE University Belgaum from 28th &29th August 2009,
Participated as delegate at National workshop on Survey of Medicinal Plants in Western Ghats” held at KLES College of Pharmacy, Belgaum, and field trip conducted at Jomboti forest area of Belgaum District.
Participated in CEP& Quality improvement program on “Rational methods for microbial development” at IIT Bombay during April 9-13, 2007.
Participated in DST sponsored six-day SERC School on “Fermentation Process Modeling, Optimization and Simulation methods” at IIT Bombay during April 10-15, 2006.
Professional memberships
Member APTI, Membership number: KA/LM-896
Member Indian Pharmaceutical Association. Membership number:KTK/BEL/LM/045
Member ADNAT Society (Association for the Promotion of DNA Fingerprinting
and other DNA Technologies)
Member International Carotenoid Society.
Additional Information
Personal Details: Date of Birth: 04-08-1971, Marital Status: Married
Submitted Research, Project proposals to DBT, AICTE, ICMR, VGST
Participated in Pre-workshop as an expert committee member for design of syllabus for M.Pharm(Pharmaceutical Biotechnology), Rajiv Gandhi University of Health sciences University, Banglore.
Participated as expert committee member for design of syllabus at KLE University for M.Pharm(Pharmaceutical Biotechnology),MSc Biotechnology, Post Graduate Diploma in Nanomedicine and Post Graduate Diploma in Medical Biotechnology, Applied Biochemistry, Pharmaceutical Biotechnology for B.Pharm semester sytem, and for M.Pharma Pharmaceutical biotechnology
Performed professional academic duties such as question Paper setting, evaluation, and conducting examinations as external and internal examiner for UG/PG B.Pharm Examinations at KLE University,JSS University, GOA University, RUGHS and Manipal university.
Performed the assigned examination duties as invigilator, room supervisor, custodian and other confidential jobs of KLE University. Also I am Internal MCQ in charge for KLE University’s College of Pharmacy, Belgaum.
I am exposed to theoretical concepts and working knowledge of research techniques and methodologies required for the field of pharmaceutical sciences and biotechnology.