ProFI- Research Project Proposal1/317/04/19

Project Code: / PROXXX
Researcher:
Contact person:
Contact e-mail:
Contact phone:
Date submitted:
1. Project title:
2. Biology background (2 sentences/ also submit 1-2 PDF files-preferably review papers):
3. Biological problem addressed by this ProFI project (2 sentences):
4. Proteomics effort - aim :
I. Intact protein analysis
  • Native mass
  • PTMs
Specify / IV. ID of subunits of protein complexes
  • Native page
  • Gel filtration
  • Other

V. Interacting protein ID after affinity purification
  • Dna Oligo bait
  • His tagged Protein
  • GST tagged Protein
  • Other

II. Interacting protein ID after immunoprecipitation
III. ID ofPTMs on a protein
Specify
5. Protein target amounts(ng, µg):
OR:
can you see your protein of interest on a gel by:
Coomassie
Silver
Western (ECL)
IMPORTANT: if you can only see your protein by western you need to scale up your prep
6. Preliminary protein characterization:
(Submit a 1-3 slide Powepoint file with gels-westerns on protein expression, purification, westerns etc plus a short description)
7. Proteomics sample complexity
  • expected no of protein targets to be identified

  • expected no of background proteins

8. Protein target nature/features :
  1. Cell localization: Cytoplasmic, Membrane, Other
  2. pI:
  3. MW (native):
  4. MW (with added recombinant tags):
  5. Cysteine Content:
  6. Potential isoforms:

for intact protein analysis only
9. Protein target buffer composition:
  1. Buffering Species:
  2. Detergent(s):
  3. pH:
  4. Ionic Strength:

10. Known protein post translational modifications of target protein(s)
Experimentally determined (reference/PDF) / Predicted by informatics (programme/url)
I. / I.
II. / II.
III. / III.
IV. / IV.
for protein interactor analysis only
11. Known Protein Interactors of Target protein (Submit corresponding PDF):
12. Previous proteomics studies (if available):
(Submit relevant PDFs)
13. In silico digestion of individual protein(s):
(perform in silico digestion of your protein with trypsin at http://prospector.ucsf.edu/prospector/cgi-bin/msform.cgi?form=msdigest and paste derived peptides in an excel file and send it with the form)
14. Access codes/Names of Protein(s) of interest (if available):
Protein Name(s):
Protein synonyms (consult: ):
Accession number (UNIPROT):
Gene name:
Locus name:
15. FASTA Sequences
A. Single protein wild type (paste sequence):
  1. Single protein recombinant (i.e. with hisTags, mutations etc; paste sequence):

  1. Whole Organism Proteome (add ftp/url below):
(IMPORTANT: make sure your protein is present in this database with an appropriate name)
  1. Sub-proteome (add ftp/url below):
(IMPORTANT: make sure your protein is present in this database with an appropriate name)
16. Notes: