Table S4. Proteomic features of Pf proteins associated with protection from uncomplicated malaria.

Gene ID / Protein Name / P * / Signal Peptide / # TM Domains / Mass Spec. Expression Evidence† / Affy Expression Evidence‡
PFI0510c / Hypothetical, conserved / 0.00002 / No / 0 / Spor / gam
PFD1060w / u5 small nuclear ribonucleoprotein-specific protein / 0.0004 / No / 0 / mero>spor>gam>troph / early troph
PFC0875w / ABC transporter / 0.0005 / No / 13 / unknown / early ring
PFA0510w / bromodomain containing protein / 0.0005 / No / 0 / gam>spor / early troph
PF14_0419 / Hypothetical, conserved / 0.0005 / No / 0 / gam>spor / early schiz
PF14_0170 / NOT family protein / 0.0008 / No / 0 / spor>mero / early schiz
PFL1545c / chaperonin cpn60 / 0.0008 / Yes / 1 / iRBCm>trop=schiz>gam / early troph
PFA0110w / RESA / 0.0009 / No / 0 / mero=troph=schiz>iRBCm>gam / mero
MAL13P1.148 / Pf myosin / 0.001 / No / 0 / troph>gam / early troph
PFL1620w / asparagine/aspartate rich protein / 0.001 / No / 0 / Spor / early troph
PF13_0179 / isoleucine--tRNA ligase / 0.001 / No / 0 / mero>gam>troph / early troph
PF11_0232 / Hypothetical, conserved / 0.001 / No / 0 / spor>gam / early troph
PF10_0356 / LSA-1 / 0.002 / Yes / 1 / liver[23] / mero
PF14_0384 / allantoicase / 0.002 / No / 0 / gam>troph / early troph
MAL13P1.278 / Ser/Thr protein kinase / 0.002 / No / 0 / spor>troph>mero / gam
PF13_0350 / signal recognition particle receptor alpha subunit / 0.002 / no / 0 / gam>mero>iRBCm / early troph
PF10_0177 / erythrocyte membrane-associated antigen / 0.002 / Yes / 1 / Troph / gam
PFI0925w / gamma-glutamylcysteine synthetase / 0.002 / No / 0 / Mero / early ring
MAL7P1.138 / Hypothetical, conserved / 0.002 / No / 5 / gam>spor / early ring
PFE0090w / chromosome assembly factor 1 / 0.002 / No / 0 / troph>mero / early schiz
PF11_0507 / antigen 332 / 0.002 / No / 0 / iRBCm=troph=schiz / early troph
PFE0055c / HSP / 0.003 / No / 1 / iRBCm>gam>troph=schiz / mero
PF14_0344 / Hypothetical, conserved / 0.003 / Yes / 0 / iRBCm>troph=schiz>mero>spor>gam / mero
MAL13P1.22 / DNA ligase 1 / 0.003 / Yes / 0 / troph>spor / early schiz
PF11_0008 / PfEMP1 / 0.003 / No / 1 / spor and iRBCm[14] / gam
PF11_0158 / Hypothetical / 0.004 / No / 0 / mero>troph=gam>spor / gam
PF11_0175 / HSP / 0.004 / Yes / 1 / troph=schiz>iRBCm>mero>gam>spor / mero
PF14_0632 / 26S proteasome subunit / 0.004 / No / 0 / iRBCm>mero=gam>spor / late troph
MAL13P1.323 / Hypothetical, conserved / 0.004 / No / 2 / spor>gam>mero=troph / early ring
PF14_0681 / diacylglycerol kinase / 0.004 / No / 0 / gam>mero / early schiz
PF13_0003 / PfEMP1 / 0.004 / No / 0 / spor and iRBCm[14] / early ring
MAL13P1.140 / Hypothetical, conserved / 0.004 / No / 0 / Spor / gam
PFI0345w / GTPase activator / 0.004 / No / 0 / Gam / early ring
PFL0470w / Hypothetical, conserved / 0.004 / No / 0 / mero>troph / late schiz
PF07_0006 / Starp antigen / 0.004 / Yes / 0 / spor[21]>gam / early ring
PF13_0190 / Hypothetical, conserved / 0.004 / No / 0 / gam>mero>iRBCm / early troph
PFB0115w / Hypothetical, conserved / 0.004 / No / 0 / troph>spor / early troph
PF13_0285 / inositol-polyphosphate 5-phosphatase / 0.004 / No / 3 / unknown / gam
PF11_0270 / threonine -- tRNA ligase / 0.004 / Yes / 0 / mero>troph=schiz>iRBCm>gam>spor / early troph
PFI0855w / Hypothetical, conserved / 0.004 / No / 0 / Gam / late troph
PF07_0035 / cg1 protein / 0.004 / Yes / 0 / iRBCm>spor / late troph
PFB0260w / proteasome 26S regulatory subunit / 0.005 / No / 0 / iRBCm>schiz=troph>mero>gam>spor / gam
PFL0545w / kinesin-related protein / 0.005 / No / 0 / spor>gam / gam
MAL13P1.56 / m1-family aminopeptidase / 0.005 / No / 1 / mero>troph=schiz>gam>iRBCm>spor / early troph
PF07_0126 / transcription factor with AP2 domain(s) / 0.005 / No / 0 / spor=troph>mero / early ring
PFL0590c / P-type ATPase / 0.005 / No / 8 / mero>spor>gam>troph / early troph
MAL6P1.131 / SET-domain protein / 0.005 / No / 0 / mero>gam>spor
PFE0060w / PIESP2 / 0.005 / Yes / 3 / iRBCm>troph=schiz>gam / early troph
PFD0430c / MAC/perforin / 0.005 / Yes / 0 / Spor / gam

*P value for the comparison of reactivity between malaria protected and susceptible children. The Benjamini-Hochberg method was used to correct for the false discovery rate.

†From PlasmoDB, Sequest algorithm ( Stage-specific expression evidence in this column that is not based on mass spectrometry evidence is noted. Abbreviations: spor=sporozoite, mero=merozoite, troph=trophozoite, schiz=schizont, iRBCm=infected red blood cell membrane, gam=gametocyte.

‡From PlasmoDB.