Revised 6 November 2005

FIELD Limnology

PBIO/ZOOL 719/819

Fall 2005

Instructors: Prof. Alan Baker (Plant Biology) Office: Spaulding 126 (2-3845)

Prof. James Haney (Zoology) Office: Spaulding 116 (2-2105)

Graduate Teaching Assistant: Sonya Carlson (Zoology) Office: Spaulding 116 (2-2105)

Undergrad Teaching Assistant: Janet Kleiner

Texts & Materials: 1. Common Methods in Limnology, by Lind (recommended)

2.   Limnoecology (Lampert & Sommer) (required)

3.   Textbook of Limnology (Cole, 4th ed.) (required)

4.   A Short Guide to Writing about Biology, by Pechenek (required)

5.   USB data storage for PC (required)

6.   Data notebook (3 ring) with plastic sheet protectors (required)

7.   Clipboard/cover for recording field data (required)

8.   Sunny smile and positive attitude (required)

Aug 30 Introduction to course: Course Objectives; Research Theme: Diel Vertical Migration (DVM)

Learning laboratory techniques: Introduction to laboratory examination and counting of zooplankton.

Sep 1 Field Trip 1: Barbadoes Pond (Dover, NH).

FOR NEXT CLASS: Stich and Lampert (1981)

Sep 6 Lab: Intro to zooplankton and data logger downloading. Computer graphing with SigmaPlot Seminar: 1) PPt presentation & interpretation of lake profiles: Barbadoes Pond trip

2) Discuss Stich & Lampert (1981)

Sep 8 Field Trip 2: Barbadoes Pond (Dover, NH). DVM collection

Arrive at 3 pm/Return at 10 pm

Sep 13 Introduction: Personal gear check for SLCFT (Sawyer Ponds Camping Field Trip)

Lab: Zooplankton Identification/Counting

Lecture: Light & Temperature

Seminar: Discussion of vertical profile plots of DVM at Barbadoes

FOR NEXT CLASS:

Read: Hansson L.A. (2004) Plasticity in pigmentation induced by conflicting threats from predation and UV radiation. ECOLOGY 85 (4): 1005-1016.

Prepare answers: Book Questions #1: L&S Chapter 3: Questions 1, 6 & 7

Sep 15 Seminar: Discuss Book Questions #1& Hansson 2004

Lecture: Solutes

Lab: Upload & analyze data: SigmaPlot, zooplankton & phytoplankton counting

FOR NEXT CLASS: Read: TBA

Prepare answers: Book Questions #2: L&S Chapter 3: Question 4; Chapter 4: Questions 1 & 3

Sep 20 Lab: Prepare for SLCFT by labeling containers

Seminar: Discuss Book Questions #2

Sep 22 Field Trip 3: Sawyer Ponds (Upper Sawyer and Lower Sawyer Ponds) –

Leave Spaulding loading dock at 8 am.

Return to Durham campus Friday, September 23 at 2 pm

Prepare answers: Book Questions #3: L&S Chapter 4: Questions 7 & 8

Sep 27 Due: Book Questions #3: L&S Chapter 4: Questions 7 & 8

Lab: Upload & analyze data: SigmaPlot, zooplankton & phytoplankton counting

Sep 29 Field Trip 3: Mendums Pond

Oct 4 Discussion: Introduction to scientific writing and manuscript review (Handouts)

Lab: Data uploading and analysis from Mendums Pond

Seminar: Discussion of profiles from Mendums Ponds

FOR NEXT CLASS:

Prepare answers: Book Questions 4: L&S L&S Chapter 4: Questions 11 & 12

Oct 6 Field Trip 4: Russell Pond

Leave Spaulding loading dock at 8 am.

Return to Durham campus late night

Oct 11 Data anaylsis: (1) Fluorometry Data Analysis

(2) Epilimnion Chlorophyll Analysis

FOR NEXT CLASS:

Prepare answers: Book Questions 5: L&S Chapter 6: 7, 8 & 9; L&S Chapter 7: 1 & 2

Oct 13 Discussion: 1) Introduction to scientific writing and manuscript review (handouts), with emphasis on Methods (make list of all methods used)

2) Introduction to statistics: data analysis using parametric and non-parametric tests, means, modes, variance and tests of significance

3) Select lakes and “emphasis” for manuscripts

4) Peek at Russell Pond sonar

Lab: Plankton counting & data uploading and analysis from Mendums Pond

Seminar: Discuss Book Questions 5

Assignment: Prepare first draft of Methods section due October 18.

Oct 18 Field Trip: Stonehouse Pond DVM

Meet at 2 pm, Return at 9:30 pm

Methods MS due

Assignment: Review two METHODS manuscripts (due October 20)

Oct 20 Lab: Plankton counting & data uploading and analysis from Russell Pond

Data analysis techniques: Regressions Introduction to the use of cladistics

Lecture: Diel Vertical Migration (Sonya Carlson)

MS Writing: Peer reviews of METHODS MS due

Assignments: 1) Write INTRODUCTION (due October 27) 2) Read Papers for Oct 27

Oct 25 Lecture: Origins and Diversity of Lakes and Watersheds

Lab: Plankton counting & data uploading and analysis from Stonehouse Pond

Data analysis: Examination of fluorescence data

Seminar: TBA

Oct 27 Lecture: Eutrophication: Causes, consequences and trophic Classification of Lakes

Lab: Chaoborus crop analysis and calculation of feeding electivity indicies

MS Writing: MS INTRODUCTION: first draft due for peer review

Seminar: Impact of Chaoborus predation

Nov 1 Lecture: Phytoplankton: Diversity and Competition

Lab: Sample processing: Computer Image analysis of plankton body lengths

Graphing of plankton profiles

MS Writing: Peer Reviews of INTRODUCTION due

Assignment: Prepare preliminary manuscript tables and figures (due Nov 8)

Nov 3 Lecture: Zooplankton Grazing: Mechanisms and Impact on Phytoplankton

Manuscript preparation: Data analysis, graphics and sample processing:

Seminar: TBA

Nov 8 Lecture: Vertebrate and Invertebrate Predation in the Pelagic Zone

Manuscript preparation: Data analysis, graphics and sample processing:

Nov 10 Lecture: Biomanipulation as a Lake Restoration Tool

Lab: Group projects on Lake Model Videos (fish selective feeding & thermal stratification)

Assignment: Review test manuscript (due Nov 17)

Manuscript tables and figures due (hardcopies, or PowerPoint files)

Nov 15 Lecture: Seasonal and Long-term Succession in Lakes

Student DVM Presentations*: Barbadoes Pond & Mendums Pond

* 15-min PowerPoint presentations of results (graphs, tables, photos, etc) from phytoplankton and zooplankton emphasis, i.e. two presentations per lake

Nov 17 Lecture: Fish and Pelagic Food Webs

Manuscript preparation

Student DVM Presentations: Russell Pond & Stonehouse Pond

Seminar: Comparision of class reviews of the test MS

Nov 22 Manuscript preparation

Seminar: TBA

Nov 24 Thanksgiving Holiday

Nov 30 Manuscript preparation

Seminar: TBA

Dec 1 *Submit DVM manuscripts to editors at 2:00 PM
Pick CLS manuscripts to review at 3:00 PM

Dec 6 1:00 PM: Reviewers return manuscripts to editors
2:10 PM Authors pick up reviewed manuscripts.

2:20 PM List what manuscript needs: Major changes, minor changes, typographical corrections

2:35 PM List graphs, information, or references needed to revise your manuscript

3:00 PM Conference with instructors to review your progress on your manuscript

4:30 PM Authors revise manuscripts and change to co-authored (CFB journal format)

*By the end of class, you are expected to have the abstract, introduction, methods, and 1 table & figure embedded into a new document.*

Dec 8 Continue to revise manuscript into co-authored DVM manuscript (CFB journal format)

Complete student faculty evaluations

Dec 15 Submit Revised DVM manuscript (CFB journal format) to CFB on-line journal (Center for Freshwater Biology Research)


Summary of Grading:

Manuscript draft

Introduction 5%

Methods 5%

Single Author Manuscript* 15%

Co-Authored Manuscript 15%

MS reviews

Introduction 5%

Methods 5%

Single Author Manuscript 10%

Student Presentations on Lake 5%

Student Lectures 10%

Book Questions @ 2 pts per hand-in 10%

Seminars 10%

Class & field trip participation 5%

TOTAL 100%

*=Single Author Manuscript has emphasis on either phytoplankton or zooplankton

Writing Timeline:--

Oct 18 Draft 1 of Methods due

Oct 20 Review of Methods due

Oct 27 Draft 1 of Introduction due

Nov 1 Review of Introduction due

Nov 22 Single Author DVM Manuscript due

Dec 2 Review of Single Author Manuscript due

Dec 15 Co-Authored Manuscript due

Text topics for student lectures (30 min):

1.  Sep 22: Regulation of Phytoplankton (Janet)

2.  Oct 6: Lakes and Watersheds

3.  Oct 11: Trophic Classification of Lakes

4.  Oct 13: Zooplankton Grazing

5.  Oct 18: Predation in the Pelagic Zone

6.  Oct 20: DVM

7.  Oct 25: Seasonal and Long-term Succession in Lakes

8.  Oct 27: Pelagic Food Webs and Microbial Loops

9.  Nov 1: Fluorescence & Chlorophyll in lakes

3