Haiqiu (Jason) Huang October 2016
Haiqiu (Jason) Huang
| (240) 472-1329 | North Bethesda, MD
HIGHLIGHTS
§ Experienced in cell biology, molecular biology, microbiology, and analytical chemistry analyses
§ Expertise on diet-induced cardiovascular disease and xenograft tumor animal models
§ Demonstrated success in designing and delivering projects assessing safety and bioactivity of nutraceuticals
§ Published 16 refereed journal articles, 3 book chapters, 15 conference presentations over the past 6 years
§ Team-player, diligent and quick learner, with a good sense of humor
§ Passionate about a career in dietary supplement industry and developing health promoting products
EDUCATION
Ph.D., Nutrition and Food Science, GPA: 4.0/4.0 December 2014
University of Maryland, College Park, Maryland, USA
Bachelor of Science, Biotechnology and Pharmacology, Top 5% June 2009
Fudan University, Shanghai, China
EXPERIENCE
John A. Milner Post-Doctoral Research Fellow Jan. 2015-present
A merit-based fellowship jointly awarded and hosted by
Beltsville Human Nutrition Research Center, USDA-ARS, and Beltsville, MD
Office of Dietary Supplements (ODS), National Institute of Health (NIH) Bethesda, MD
Research Duties and Accomplishments:
§ Designed novel studies on effects of microRNAs and phytochemicals on gut microbiota.
o Identified candidate plant microRNAs targeting human gut microbiota genome using in-silico/bio-informatics analyses; Invited guest speaker at Beltsville Area Research Center, USDA-ARS (June 2015);
o Developed in-vitro models and animal feeding studies to evaluate plant microRNAs and phytochemicals’ effect on the population and composition of gut microbiota;
o Prepared bacterial DNA for metagenomics and qRT-PCR of gut microbiota analysis;
o Improved and validated method for microRNA methylation status analysis in plant and animal matrices.
§ Evaluated the safety and efficacy of functional foods and phytochemicals in reducing diet induced metabolic and gut microbiome changes, chronic inflammation, and cancer prevention using multiple rodent models.
o Developed animal study protocols and diet for diet-induced obesity and xenograft tumor models, balanced calorie and supplementation levels of phytochemicals;
o Analyzed expression levels of critical genes in cholesterol and lipid metabolism, inflammation, tumor growth and progression, and transcription factor in liver, adipose, spleen, tumor etc.;
o Identified acute toxicity of I3C resulting in morphological changes in intestine and determined the molecular mechanisms using Immunohistochemistry and gene expression assays;
o Used pathway analyses and bioinformatics tools for constructing cholesterol metabolism pathway in a hamster model and correlation analysis of gene expressions and physiological outcomes.
Regulatory Duties and Accomplishments (at ODS):
§ Trained in the translation of nutrition science to policies, and facilitated communications of evidence-based information of dietary supplements researches to the public.
o Identified gaps in government endorsed, up-to-date information of dietary supplements on regulation, efficacy, safety, and interaction with food/drug/other botanicals;
o Conducted a survey on healthcare professionals’ awareness of dietary supplement use among patients/clients and the need for information from ODS, and presented summary report to senior staff;
o Participated and contributed to 2015 and 2016 ODS scholar intramural grant application review.
Graduate Research Fellow May 2010-Dec. 2014
Nutraceuticals and Functional Food Lab, University of Maryland, and College Park, MD
Beltsville Human Nutrition Research Center, USDA-ARS Beltsville, MD
Research Roles and Accomplishments:
§ Participated in four animal feeding studies using athymic nude mouse xenograft models to determine cancer preventative effects of phytochemicals.
o Prepared human prostate cancer cells for tumor injection and established xenograft models;
o Processed tumor and liver RNA, and constructed dsDNA library for RNA-Seq analysis;
o Analyzed plasma concentrations of phytochemicals using HPLC and mass spectrometry.
§ Conducted three studies on effects of botanical extracts and phytochemicals in reducing lipid and cholesterol level using golden Syrian hamster feeding model.
o Modified and improved fermentation conditions to increase levels of soy phytochemicals, and isolated three compounds using fractionation extraction and preparative HPLC;
o Determined lipoprotein levels in plasma, cholesterol and lipid levels in liver and fecal samples, and designed and validated gene expression assays for hamster.
§ Optimized and validated lipopolysaccharide (LPS) stimulated inflammatory response in J774A.1 mouse macrophage, THP-1 human monocyte and differentiated monocyte to simulate physiological conditions.
o Characterized the temporal and dose-dependent response of inflammatory cytokines and chemokines in transcriptional and translational levels;
o Identified treatment conditions and time points of maximum activation in different cell models for optimal response in anti-inflammation analysis.
§ Isolated and identified two phytochemicals from leaves of E. roxburghiana, and assessed in-vitro antiproliferative and anti-inflammatory effects.
Lab Management Roles and Accomplishments:
§ Trained and supervised students and visiting scholars on cell and molecular biology techniques.
§ Facilitated lab operations, and streamlined order procedures to reduce monetary and chemical waste.
§ Participated in drafting and preparing 6 grant proposals submitted to USDA NIFA and NIH.
Visiting Scholar Jan.-Feb. 2012 & May-Aug. 2013
School of Agriculture and Biology, Shanghai Jiao Tong University Shanghai, China
§ Led the initial set-up of a new scientific cell and molecular biology laboratory and an animal facility.
o Responsible for the procurement and placement of key equipment, supplies, biological grade reagents, and consumables to ensure efficient workflow and avoid contamination;
o Established and standardized animal care, housing, hygiene, feeding, and operating conditions to meet the AAALAC standards to ensure animal welfare and study quality and reproducibility;
o Standardized assay protocols and lab operations;
o Trained facility staff and students on good practice, contamination avoidance, and related techniques.
TEACHING
Teaching Assistant Sept. 2011-Dec. 2014
Department of Nutrition and Food Science, University of Maryland College Park, MD
NFSC421 – Food Chemistry, Fall 2011, 2012, 2013, 2014
o Lectured selected topics in Food Chemistry (16 lectures).
o Maintained course website to assist smooth communication.
o Collected and distributed Food Chemistry related information.
o Graded exams, term papers and presentations.
NFSC100 – Elements of Nutrition, Spring 2013
o Prepared materials and lectured discussion sections.
o Collected and distributed human nutrition related information.
SKILLS & TECHNIQUES
§ Molecular biology: DNA, RNA and microRNA isolation from mammalian, plant and bacterial cells, and animal tissues; DNA, RNA and microRNA quality analyses (Nanodrop, Bioanalyzer, Experion); PCR; Real-Time PCR; Microarray; Taqman Low Density Arrays (TLDA) analysis; Protein assay (Bicinchoninic acid assay; ELISA; Western Blot; Luminex® Assay); Immunohistochemistry assays; TUNEL assay; DNA ligation; E. Coli transformation; Gel electrophoresis.
§ Cell biology: Mammalian cell culture; siRNA and gene silencing; Transfection and stable cell line generation; Cell based anti-oxidant assay; Luminescence ATP detection assay; Sulforhodamine B colorimetric assay; Hemacytometer; Cryopreservation.
§ DNA and RNA sequencing: DNA and RNA sample preparation; Library construction; cDNA quality analysis; HiSeq 2500 Ultra-High-Throughput Sequencing System; MiSeq Sequencing System.
§ Animal model: Xenograft model; Tumor volume measurement; Intraperitoneal and tail vein injection; Cardiac puncture blood collection; Routine animal care; Plasma and tissue collection; Sample size and power calculation; Diet design and calorie calculation; Human-animal dose translation and calculation.
§ Microbiology: Aerobic and anaerobic bacterial cell culture; Sterilization; Aseptic techniques; Agar and broth inoculation; Streaking.
§ Analytical chemistry: HPLC (Shimadzu Prominence UFLC system, Agilent 1260 infinity HPLC); Mass spectrometry (Waters Quadrupole Time-of-flight Mass Spectrometry Xevo G2 QTOF, Tandem Quadrupole Mass Spectrometry Xevo TQS).
§ Pathway and statistical analysis: IPA (Ingenuity Pathway Analysis); CLC Genomic Workbench (QIAGEN Bioinformatics); RDP Pipeline; IBM SPSS Statistics; SAS (Statistical Analysis System); GraphPad Prism; BLAST (gene and primer); ABI PRISM Primer Express.
§ Others: Soxhlet extraction; Fractionation; Purification (thin-layer chromatography); Lipid and cholesterol extraction; Amplex® Red Cholesterol Assay; High-throughput colorimetric, fluorometric, and UV assays; Rotor evaporation; Nitrogen evaporation; Freeze-drying.
HONORS & AWARDS
§ 2016 First place in the American Society for Nutrition’s Emerging Leaders in Nutrition Science Competition, Experimental Biology Annual Meeting
§ 2015 John A. Milner Postdoctoral Research Fellowship, Beltsville Human Nutrition Research Center, USDA-ARS and Office of Dietary Supplements, NIH
§ 2014 First Place in Graduate Research Competition, School of Agriculture and Natural Resources, University of Maryland
§ 2009-2014 Full Graduate Assistantship, University of Maryland
§ 2013 International Graduate Research Fellowships, University of Maryland
§ 2013 Nutrition and Food Science Graduate Research Competition Award, University of Maryland & Capital Area Food Protection Association
§ 2012 Golden Key Research Grant Award, Golden Key International Honour Society
§ 2011, 2012, & 2014 Jacob K. Goldhaber Travel Award, University of Maryland
§ 2009 Thermo-Fisher Scientific Scholarship, Fudan University
§ 2006-2009 Renmin National Scholarship, Fudan University
PROFESSIONAL MEMBERSHIP
§ Institute of Food Technologists (ID # 00478583), 2009-present
§ Golden Key International Honour Society, University of Maryland Chapter (ID # 8681480), 2011-present
§ American Society of Nutrition (ID # 44668), 2013-present
§ American Association for the Advancement of Science (ID # 20335316), 2015-present
PUBLICATION
Refereed Journal Articles
1. Huang, H., Krishnan, H., Pham, Q., Yu, L., & Wang, T. (2016) Soy and Gut Microbiota: Interaction and Implication for Human Health. Journal of Agricultural and Food Chemistry, in revision.
2. Jiang, X., Huang, H., Xiao, Z., Yu, L., Pham, Q., Yokoyama, W., Yu, L., Luo, Y., & Wang, T. (2016) Red cabbage microgreen lower circulating LDL, liver cholesterol and inflammatory cytokines in mice fed a high fat diet. Journal of Agricultural and Food Chemistry, in revision.
3. Huang, H., Fletcher, A., Yu, L., Pham, Q., Yu, L., & Wang, T. (2016) Reversible toxic effects of the dietary supplement indole-3-carbinol in an immune compromised rodent model: intestine as the main target. Journal of Dietary Supplements, in press.
4. Liu, M., Liu, J., Wu, Y., Gao, B., Wu, P., Shi, H., Sun, X., Huang, H., Wang, T., & Yu, L. (2016) Preparation of five 3-MCPD fatty acid esters, and the effects of their chemical structures on acute oral toxicity in Swiss mice. Journal of the Science of Food and Agriculture, in press.
5. Huang, H., Xie, Z., Yokoyama, W., Yu, L., & Wang, T. (2016). Identification of liver CYP51 as a gene responsive to circulating cholesterol in a hamster model. Journal of Nutritional Science, 5, 1-12.
6. Yu, L., Huang, H., Yu, L., & Wang, T. (2014) Utility of hesperidinase for food function research: enzymatic digestion of botanical extracts alters cellular antioxidant capacities and anti-inflammatory properties. Journal of Agricultural and Food Chemistry, 62, 8640-8647.
7. Zhang, X., Shang, P., Qin, F., Zhou, Q., Gao, B., Huang, H., Yang, H., Shi, H., & Yu, L. (2013). Chemical composition and antioxidative and anti-inflammatory properties of ten commercial mung bean samples. LWT-Food Science and Technology, 54, 171-178.
8. Huang, H., Xie, Z., Boue, S., Bhatnagar, D., Yokoyama, W., Yu, L., & Wang, T. (2013). Cholesterol lowering activity of soy-derived glyceollins in the golden Syrian hamster model. Journal of Agricultural and Food Chemistry, 61, 5772−5782.
9. Shi, H., Yang, H., Zhang, X., Sheng, Y., Huang, H., & Yu, L. (2012). Isolation and Characterization of Five Glycerol Esters from Wuhan Propolis and Their Potential Anti-Inflammatory Properties. Journal of Agricultural and Food Chemistry, 60, 10041-10047.
10. Zhang, X., Gao, B., Shi, H., Slavin, M., Huang, H., Whent, M., Sheng, Y., & Yu, L. (2012). Chemical Composition of 13 Commercial Soybean Samples and Their Antioxidant and Anti-inflammatory Properties. Journal of Agricultural and Food Chemistry, 60, 10027-10034.
11. Xin, W., Man, X., Zheng, C., Jia, M., Jiang, Y., Zhao, X., Jin, G., Mao, Z., Huang, H., & Qin, L. (2012). Prenylated phloroglucinol derivatives from Hypericum sampsonii. Fitoterapia, 83(8), 1540–1547.
12. Huang, H., Fletcher, A., Niu, Y., Wang, T., Yu, L. (2012). Characterization of Lipopolysaccharide-stimulated Cytokine Expression in Macrophages and Monocytes. Inflammation Research, 61(12), 1329-1338.
13. Whent, M., Huang, H., Xie, Z., Lutterodt, H., Yu L., Fuerst, E. P., Morris, C. F., Yu, L., & Luthria, D. (2012). Phytochemical Composition, Anti-inflammatory, and Antiproliferative Activity of Whole Wheat Flour. Journal of Agricultural and Food Chemistry, 60, 2129-2135.
14. Lv, J., Huang, H., Lu, Y., Whent, M., Niu, Y., Shi, H., Wang, T., Luthria, D., Charles, D., Liu, L., & Yu, L. (2011). Phenolic composition and nutraceutical properties of organic and conventional cinnamon and peppermint. Food Chemistry, 132(3), 1442-1450.
15. Xin, W., Huang, H., Yu, L., Shi, H., Sheng, Y., Wang, T., & Yu, L. (2011). Three New Flavanonol Glycosides from Leaves of Engelhardtia roxburghiana, and their Anti-inflammation, Antiproliferative and Antioxidant Properties. Food Chemistry, 132(2), 788-798.
16. Xie, Z., Huang, H., Zhao, Y., Shi, H, Wang, S., Wang, T., Chen, P., & Yu, L. (2011). Chemical Composition and Anti-proliferative and Anti-inflammatory Effects of the Leaf and Whole-plant Samples of Diploid and Tetraploid Gynostemma pentaphyllum. Food Chemistry, 132(1), 125-133.
17. Huang, H., Cheng, Z., Shi, H., Xin, W., Wang, T., & Yu, L. (2011). Isolation and Characterization of Two Flavonoids, Engeletin and Astilbin, from the Leaves of Engelhardia roxburghiana and Their Potential Anti-inflammatory Properties. Journal of Agricultural and Food Chemistry, 59(9), 4562-4569.
18. Xie, Z., Liu, W., Huang, H., Slavin, M., Zhao, Y., Whent, M., Blackford, J., Lutterodt, H., Zhou, H., Chen, P., Wang, T., Wang, S., & Yu, L. (2010). Chemical Composition of Five Commercial Gynostemma pentaphyllum Samples and Their Radical Scavenging, Antiproliferative, and Anti-inflammatory Properties. Journal of Agricultural and Food Chemistry, 58(21), 11243-11249.
Refereed Book Chapters
1. Huang, H., and Yu, L. (2014) Food safety chemistry-an overview, in Food Safety Chemistry (eds Yu, L., Sun, B., and Wang, S.), Taylor & Francis, London, UK. ISBN 9781466597945.
2. Reddy Jala, R. G., Zhang, X., Huang, H., Gao, B., Yu, L., and Xu, X. (2014) 3-MCPD Fatty Acid Esters: Chemistry, Safety, and Technological Approaches for Their Reductions, in Food Safety Chemistry (eds Yu, L., Sun, B., and Wang, S.), Taylor & Francis, London, UK. ISBN 9781466597945.
3. Luthria, D., Ghatak, R. and Huang, H. (2012) Phenolic Phytochemicals from Rye (Secale Cereale L), in Cereals and Pulses: Nutraceutical Properties and Health Benefits (eds Yu, L., Tsao, R., and Shahidi, F.), Wiley-Blackwell, Oxford, UK. doi: 10.1002/9781118229415.ch6
Refereed Conference Presentations
1. Huang, H., Davis, C., Yu, L., Wang, T. (2016) Dietary Small RNA Modulation of Gut Microbiota Composition: in-silico and in-vitro Analyses. Experimental Biology Annual Meeting 2016, April 1-6, San Diego, CA.
2. Huang, H., Roman Arocho1, G. M, Davis, C., Yu, L., Wang, T. (2016) Consumption of Selected Cruciferous Vegetables and Soy Phytochemical Dietary Supplements Can Alter Gut Microbiome Composition. Experimental Biology Annual Meeting 2016, April 1-6, San Diego, CA.
3. Jiang, X., Huang, H., Xiao, Z., Yu, L., Pham, Q., Yu, L., Luo, Y., Wang, T. (2016) Lipids and Cholesterol-Lowering Activity of Red Cabbage Microgreens. Experimental Biology Annual Meeting 2016, April 1-6, San Diego, CA.
4. Huang, H., Xie, Z., Yokoyama, W., Boue, S., Yu, L., Wang, T. (2015) Molecular Analysis of Cholestyramine Treatment in Hamster Model Identified CYP51 as Cholestyramine-Responsive Gene. Experimental Biology Annual Meeting 2015, March 28-31, Boston, MA.