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Mode of Presentation: / Oral Presentation / Poster Presentation
Topic: / Soil Fertility and Plant Nutrition / Soil Chemistry
Soil and Water Conservation / Soil Salinity
Soil Microbiology and Biochemistry
Soil Physics / Litho-/Hydro-/Atmo-sphere Pollution
Other
Title: (Font: Times New Roman; Size: 10 Bold)
Improving the efficacy of Rhizobium inoculation in mung bean through co-inoculation with rhizobacteria containing ACC-deaminase
Author(s): (Font: Times New Roman ; Size: 10 Italic) Indicate the presenting author with an asterisk (*)
Maqshoof Ahmad*1, Zahir Ahmad Zahir1, Muhammad Abubakar Siddique2 and Muhammad Usman3
Affiliation(s): (Font: Times New Roman ; Size: 8 Normal)
1Institute of Soil and Environmental Sciences, University of Agriculture, Faisalabad, Pakistan
2University of Punjab, Lahore
3 Ayub Agricultural Research Institute, Faisalabad
Corresponding Author:
Email: / Phone: +92-41-9201092 / Fax: +92-41-2409585
Abstract: (Font: Times New Roman; Size: 10 Normal) The abstract should not exceed 300 words and must include a clear definition of the problem with objectives, methodology and expected results. Please, save the document as Microsoft Word format (No PDF please)
It is well established that higher levels of ethylene are produced in plants in response to biotic and abiotic stresses, which inhibit root growth and nodulation. Some PGPR are capable of lowering ethylene levels through an enzyme ACC-deaminase and therefore, result in better growth and nodulation in legumes. In the present study, 25 strains each of PGPR containing ACC-deaminase and rhizobia were isolated from the rhizosphere soil samples and nodules of mung bean plants on DF-minimal salt media containing ACC-deaminase as sole source of N and yeast extract manitol agar media, respectively. The first experiment was conducted on mung bean under axenic conditions to screen rhizobacteria containing ACC-deaminase for their growth promoting activity. On the basis of growth promoting activities for different growth variables (shoot length, root length, and fresh weight of root and shoot), five most effective rhizobacterial isolates (R6, K6, M2, F2 and F6), were selected for further experimentation. In the second experiment rhizobial isolates were screened for their growth promoting activity and nodulation potential in mung bean under axenic conditions. Results revealed that separate application of plant growth promoting rhizobacteria containing ACC-deaminase and rhizobia enhanced the plant growth. However, their combined application produced more pronounced effects and the combination MN6F2 increased the root length, root fresh weight, root dry weight, shoot length, shoot fresh weight, shoot dry weight of mung bean seedlings grown under axenic conditions compared with un-inoculated control. The co-inoculation also significantly enhanced the nodulation in mung bean and the maximum number of nodules plant-1, nodule fresh weight and nodule dry weight were observed in case of the combined application of rhizobial isolate MN6 and PGPR strain F2. The results imply that combined application of PGPR containing ACC-deaminase and rhizobia could be a useful approach for improving growth and nodulation in mung bean.

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17th International Congress of Soil Science, March 13-15, 2018, Faisalabad-Pakistan

Organized by: Soil Science Society of Pakistan.