BIOT
DIVISION OF BIOCHEMICAL TECHNOLOGY
Final Program, 234th ACS National Meeting, Boston , MA , August 19-23, 2007
W. Chen, W. Wang, and A. S. Rathore, Program Chairs
SUNDAY MORNING
Section A
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Stem Cells: Quantifying, Modeling and Controlling Stem Cell Fate
J. M. Piret, Organizer
8:20 —1. Behavior-activated cell selection: Application to stem cell expansion. B. Dykstra, J. Ramunas, D. Kent, C. J. Eaves, E. Jervis
8:50 —2. Tracking stem cell fate using a cell-based microscale platform. T. G. Fernandes, S. J. Kwon, M -Y. Lee, M. M. Diogo, C. Lobato da Silva, J. M. Cabral, J. S. Dordick
9:05 —3. Exploiting the synergy between lysate and acoustic standing wave fields to increase the retroviral transduction of suspension cells. P. R. Beauchesne, V. S. Tayi, B. D. Bowen, J. M. Piret
9:20 — Intermission.
9:40 —4. Self-assembly of multipotent adult progenitor cells (MAPCs) and differentiation to the hepatic lineage. K. Subramanian, K. Pauwelyn, C. Verfaillie, W -S. Hu
9:55 —5. 3-D differentiation model of ex vivo megakaryopoiesis. Y. Leysi-Derilou, N. Pineault, C. Duchesne, J -F. Boucher, A. Garnier
10:10 —6. P53 tumor suppressor protein affects hematopoietic stem cell differentiation in the megakaryocyte compartment. P. G. Fuhrken, P. Apostolidis, S. Lindsey, W. M. Miller, E. T. Papoutsakis
10:25 —7. Differentiation of adult human stem cells guided by mechano-sensing of matrix elasticity. F. Rehfeldt, A. J. Engler, D. E. Discher
Section B
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Biophysical and Biomolecular Symposium: Protein Stability
E. J. Fernandez, N. Rathore, C. R. Middaugh, A. Blake-Haskins, and A. Blake-Haskins, Organizers
8:00 — Introductory Remarks.
8:05 —8. Use of biophysical techniques for manufacturability assessment of therapeutic proteins. C. Li, Y. Jiang, J. Svitel, J. Wen, R. Ramachander, S. Wang, J. Li, L. Narhi
8:25 —9. A novel bioseparation approach: Use of chaotropic agents for HIC and ion exchange chromatography to dissociate noncovalent multimers during purification of an Fc fusion protein. A. B. Magill II
8:45 —10. Aggregation of a monoclonal antibody induced by adsorption to microparticle surfaces. J. Bee, J. Stevenson, K. Chatterjee, E. Freund, J. F. Carpenter, T. W. Randolph
9:05 —11. Assessment of protein stability during freeze-thaw process. K. Gupta, N. Rathore, L. Barron, W. Ji, F. Jameel, K. Murphy
9:25 — Intermission.
9:45 —12. Identifying optimal solution conditions for high concentration protein formulations: Use of self-interaction chromatography. M. C. Manning, C. S. Henry, J. J. Valente, R. W. Payne, W. W. Wilson
10:05 —13. Stability of high concentration rhuMAb VEGF. H. Liu, M. E. M. Cromwell
10:25 —14. Stability of protein antigens in vaccine delivery formulations. J. D. Chesko, J. Kazzaz, P. Malyala, K. Patton, M. Ugozzoli, D. O'Hagan, M. Singh
10:45 —15. Tungsten, prefilled syringes and protein aggregation. R. Swift, Y. Nashed-Samuel, W. Liu, L. Narhi, J. Davis
11:05 — Concluding Remarks.
Section C
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Biophysical and Biomolecular Symposium: Protein Folding & Characterization
K. Mallela and T. Cellmer, Organizers
8:00 —16. Hidden folding intermediates in small proteins: Implications for the folding energy landscape, cooperativity, and evolution of protein structures. Y. Bai
8:25 —17. Electrostatic properties of the unfolded state: What can we learn from modeling? N. C. Fitzkee, B. García-Moreno E
8:45 —18. Ultrafast folding of the villin subdomain. T. Cellmer, W. A. Eaton, J. Hofrichter
9:05 —19. Simultaneous and systematic evaluation of multiple protein refolding conditions. P. A. Leland
9:25 — Intermission.
9:45 —20. Driving forces for protein folding on the ribosome as a function of polypeptide chain elongation: Experimental and computational approaches. S. Cavagnero, J. P. Ellis, B. C. Mounce, N. Kurt
10:10 —21. Joining NMR and simulation to capture Alzheimer's Aβ monomer and fibril structural ensembles. N. L. Fawzi, T. Head-Gordon
10:30 —22. Denaturant and isotopic effects on stability and folding of interleukin-1 receptor antagonist. R. F. Latypov, D. Liu, T. Kohno, D. N. Brems, A. A. Raibekas
10:50 —23. Protein folding principles: Foldons, sequential stabilization, and optional errors. K. Mallela, S. W. Englander
Section D
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Upstream Processing: Advances in Biocatalysis
P. C. Cirino and L. Sun, Organizers
8:00 —24. A blue fluorescent protein with oxidoreductase activity. K. M. Polizzi, D. A. Moore, A. S. Bommarius
8:20 —25. A novel laccase/redox mediator system increases space-time yields and operational stability in dehydrogenase reactions. R. Ludwig, D. Haltrich
8:40 —26. Alginate-derived nanovolume cytochrome P450 microarrays for high-throughput inhibition assays. S. M. Sukumaran, M -Y. Lee, D. S. Clark, J. S. Dordick
9:00 —27. Evolution in reverse: Engineering a xylose-specific xylose reductase. N. Nair, H. Zhao
9:20 — Intermission.
9:40 —28. Directed evolution of LuxI for enhanced synthesis of OHHL. P. K. R. Kambam, L. Sun
10:00 —29. Biocatalytic systems for aromatic oxidations. A. M. McIver, S. V. B. J. Garikapati, T. L. Peeples
10:20 —30. Design of an enzyme-chaperone chimera as a new approach to enzyme immobilization and self-renaturation. L. M. Bergeron, T. Tokatlian, D. S. Clark
10:40 —31. Protein dissection and shuffling for generating recombined proteins. Y. Chen, T. Chen, H. Hua, Z. Lin
Section E
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Alan S. Michaels Recovery Award Lecture
W. Zhou, Organizer
11:30 — Introductory Remarks.
11:35 —32. Early steps in protein recovery: The messy part of a clean industry. M -R. A. Kula
SUNDAY AFTERNOON
Section A
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Stem Cells: Stem Cell Based Tissue Engineering
T. McDevitt, T. McDevitt, and P. W. Zandstra, Organizers
2:00 —33. Generation and characterization of keratinocytes from human embryonic stem cells. C. M. Metallo, L. Ji, J. J. De Pablo, S. P. Palecek
2:30 —34. Characterization of embryonic stem cells' differentiation into insulin-producing endocrines cells in 3-D cultures. X. Wang, K. Ye
2:45 —35. A novel method to control differentiating human embryonic stem cell (hESC) aggregate size and composition: Effects on cardiomyocyte development. C. L. Bauwens, R. Peerani, S. Niebruegge, K. Kolodziejska, M. Husain, P. W. Zandstra
3:00 — Intermission.
3:15 —36. Serum-free expansion of embryonic stem cells in a bioreactor for tissue engineering applications. D. E. Kehoe, L. T. Lock, E. S. Tzanakakis
3:30 —37. Hypoxic effect on hematopoietic development in human embryonic stem cell-derived embryoid bodies. C. M. Cameron, W -S. Hu, D. S. Kaufman
Section B
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Downstream Processing: Primary Recovery - Membranes and Other Technologies
D. J. Roush and Y. Lu, Organizers
2:00 — Introductory Remarks.
2:05 —38. Engineering the adsorption of plasmid DNA. W. J. Kelly, P. Butts
2:25 —39. Creating a robust and reproducible recovery process using centrifugation for the recovery of a soluble fusion protein in Saccharomyces cerevisiae. K. Herzog, J. Barringer, C. Brady
2:45 —40. Ideal membrane cascades for downstream processing. E. N. Lightfoot, J. L. O'Dell
3:05 — Intermission.
3:15 —41. Optimization of cell culture harvest clarification using an ultrascale down model for centrifugation. M. Menon, A. Hamilton, F. Riske
3:35 —42. Effect of solid ejection size on continuous centrifugation during mammalian cell culture product recovery. X. Zhao, J. Zhou
3:55 —43. Case study: Optimization of harvest stream clarification steps of a commercially-relevant fusion protein mammalian cell culture using charged depth filters. R. Bates, J. Ma, C. Mason
Section C
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Biophysical and Biomolecular Symposium: Protein Folding & Characterization
K. Mallela and T. Cellmer, Organizers
2:00 —44. All-atom computer simulation of amyloid fibrils disaggregation. C. Tan, H -F. Chen, R. Luo
2:25 —45. Dynamics of intramolecular contact formation in islet amyloid polypeptide (IAPP). S. Vaiana, W. A. Eaton, R. Ghirlando, J. Hofrichter
2:45 —46. Folding of the β-helix domain of P22 tailspike protein. M. L. Spatara, C. J. Roberts, A. S. Robinson
3:05 — Intermission.
3:15 —47. Folding and insertion of melittin in membrane mimics. M. R. Hartings, H. B. Gray, J. R. Winkler
3:35 —48. Understanding the molecular mechanism of Hsp90: The role of nucleotide hydrolysis in the chaperone cycle. K. A. Krukenberg, F. Foerster, A. Sali, D. A. Agard
3:55 —49. Breaking the symmetry of a homodimeric enzyme: Engineering and characterization of monomeric disulfide isomerases. S. A. Arredondo, G. Georgiou
Section D
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Emerging Technologies: Nanobiotechnology
Cosponsored with ANYL, and BIOHW
J. R. Cochran and L. Malmberg, Organizers
2:00 —50. Co-assembly of genetically-modified bacteriophages and inorganic nanoparticles into silica-stabilized nanoarchitectures. C. E. Ashley, E. C. Carnes, L. T. White, Z. Yuan, D. R. Dunphy, D. N. Petsev, P. Atanassov, D. Peabody, C. J. Brinker
2:20 —51. Biofabrication with genetically modified viral nanotemplates. H. Yi, G. W. Rubloff, G. F. Payne, W. E. Bentley, J. N. Culver
2:40 —52. Engineering stability and self-assembly in a caged protein scaffold. S. Lim, M. Dalmau Mallorqui, C. I. Ruiz, S -W. Wang
3:00 — Intermission.
3:10 —53. Nanoscale reservoirs for spatially and temporally controlled biointerfaces. N. A. Melosh, J. J. VanDersarl, E. Hagar-Barnard, E. Yenilmez
3:30 —54. Biological fabrication of nanostructured silicon-germanium photonic crystals possessing unique photoluminescent and electroluminescent properties. G. L. Rorrer, C. Jeffryes, D. Lee, T. Qin, C -H. Chang, T. Gutu, J. Jiao, R. Solanki
3:50 —55. Biologically programmed synthesis of hybrid semiconductor nanocrystals. S. Singh, N. Myung, A. Mulchandani, W. Chen
Section E
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Commercialization of Biologics: Case Studies: Post-Approval Changes & Comparability Challenges
S. Lee and V. Paradkar, Presiding
2:00 — Introductory Remarks.
2:05 —56. Post approval changes for a commercial process: An historical look. C. Heath, H. Madani
2:25 —57. Case study: Downstream purification support of a cell culture raw material change for a licensed mAb product. F. Maslanka
2:45 —58. Rituximab post approval changes and comparability assessments. R. Kiss, S. R. Chary, S. Meier, J. C. Goodrick, D. C. Andersen
3:05 — Intermission.
3:15 —59. Demonstrating comparability for post approval CMC changes. R. Harris
3:35 —60. Comparability protocol to support a chromatography resin change. P. J. Kramer, R. Frankenberg, R. Bhat, P. Burd, A. Hetherington
3:55 —61. Investigation of an ultrafiltration yield problem for a conjugate vaccine. S -M. Lee, B. Kruse, A. Robinson, J. Davis, C. Donaldson
4:15 — Concluding Remarks.
Section F
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Perlman Lecture
W. Zhou, Organizer
4:30 —62. Prion proteins and how they fold. S. Lindquist
Chemical Approaches to Protein Function
Sponsored by BIOL, Cosponsored with ANYL, BIOT, MEDI, BTEC, and BIOHW
MONDAY MORNING
Section A
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Emerging Technologies: Nanobiotechnology
Cosponsored with ANYL, and BIOHW
J. R. Cochran and L. Malmberg, Organizers
8:00 —63. Inhibiting bacterial biofilm formation by self-assembled monolayers of functional alkanethiols on gold. S. Hou, E. A. Burton, K. A. Simon, D. Blodgett, Y -Y. Luk, D. Ren
8:20 —64. Development of electrospun scaffolds for application to medical devices. R. Delaporte, H. Yesilalan, R. Skowyra, G. Cadd
8:40 —65. Polymersome encapsulated hemoglobin: A novel type of hemoglobin-based oxygen carrier. A. F. Palmer
9:00 —66. A novel, biomimetic hydrogel construct to repair the cornea: Molecular design and biological response. D. Myung, J. R. Cochran, J. Noolandi, C. N. Ta, C. W. Frank
9:20 — Intermission.
9:35 —67. Multifunctional titanium-binding peptides for rapid modification of implant surfaces. X. Khoo, D. J. Kenan, M. W. Grinstaff
9:55 —68. Effects of platinum and gold nanoparticles on osteoblast cells: A study of nanoparticles' cancer treatment potential. Y. Sun, D. Katz, T. Fraczak, N. Pernodet, M. Rafailovich, J. Sokolov
10:15 —69. Degradable nanoparticle gene carriers for efficient and versatile nonviral gene delivery. S. Lu, Y. J. Kwon
10:35 —70. Assembly of PEI/DNA nanoparticles for gene delivery by microfluidic hydrodynamic focusing. C -G. Koh, Z. Fei, J. Guan, Y. Xie, L. J. Lee
Section B
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Downstream Processing: Advances in Chromatography
A. M. Lenhoff and S. Ghose, Organizers
8:00 —71. Are high capacity and high selectivity mutually exclusive? New approaches to the chromatographic resin game. J. Rasmussen
8:20 —72. Microstructure and retention properties of dextran-grafted agarose media. H. Koku, B. D. Bowes, A. M. Lenhoff
8:40 —73. Use of displacement chromatography for proteomic applications. S. T. Evans, A. S. Freed, M. Platt, S. M. Cramer
9:00 —74. High performance chromatofocusing as a novel analytical method to support process comparability and product characterization. X. Kang, J. Kutzko, M. Hayes, D. Frey
9:20 — Intermission.
9:40 —75. Membrane adsorber technology for trace impurity removal applications. M. W. Phillips, C. Teske, R. Specht, M. Kozlov, W. Moya
10:00 —76. Validation of host cell protein removal in purification processes of biopharmaceutical manufacturing. C. Jiang, J. Ma, M. Rubacha, S. Lee, A. A. Shukla
10:20 —77. Profiling metal binding E. coli proteins: Its use in purification process development. R. Varakala, R. Haley, R. Henry, M. M. Ataai, R. R. Beitle
10:40 —78. Affinity adsorption of viruses using small peptide ligands. C. L. Heldt, P. V. Gurgel, L -A. Jaykus, R. G. Carbonell
Section C
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Upstream Processing: Advances in Microbial Fermentation Process Development
D. Ren, M. Laird, and X. Yang, Organizers
8:00 — Introductory Remarks.
8:10 —79. Alleviation of extracytoplasmic stress for improving cell physiology and recombinant protein production in Escherichia coli. C. P. Chou
8:30 —80. Metabolic activities of novel inducible fermentations for recombinant therapeutic proteins. M. Berge, R. Georgescu
8:50 —81. Fast track first-in-human development of novel therapeutic proteins with a robust microbial fermentation platform. X. Yang, B. Pendleton
9:10 —82. Using the two population theory to design an inducer feeding schedule to maximize recombinant DNA protein expression in Escherichia coli. J. M. Gonzalez, J. T. Hsu
9:30 —83. Mixture design for optimization of fermentation medium for cutinase production from Colleotrichum lindemuthianum. V. Shah, F. J. Rispoli
9:50 —84. Efficient antibody production with suppressing O-Glycosylation in yeast. K. Kobayashi, K. Kuroda, H. Tsumura, T. Komeda, Y. Chiba, Y. Jigami, K. Ichikawa, K. Nonaka, T. Suzuki
10:10 — Concluding Remarks.