Table S1

A list of 42 LOXs retrieved from public databases and TaLOX3 reported in this work a

Species / LOX b / UniProt accession number / Number of residues
Rice (Oryza sativa ssp japonica) / LOX1_ORYSJ, (Os03g49260) / Q76122 / 863
LOX2_ORYSJ, (Os03g52860) / P29250 / 870
LOXC1_ORYSJch, (Os08g39840) / P38419 / 924
LOX6_ORYSJch, (Os03g08220) / Q8H016 / 918
LOX4_ORYSJ, (Os03g49380) / Q53RB0 / 877
LOX3_ORYSJ, (Os03g49350) / Q7G794 / 866
LOXC2_ORYSJch, (Os08g39850) / Q84YK8 / 941
Q2QNN5_ORYSJch, (Os12g37260) / Q2QNN5 / 922
Q0IS17_ORYSJ, (Os11g36719) / Q01S17 / 868
Q6H7Q6_ORYSJch, (Os02g10120) / Q6H7Q6 / 926
Q5W6Y6_ORYSJ, (Os05g23880) / Q5W6Y6 / 395
Brachypodium distachyon / LOX1_BRADI / 689
LOX2_BRADI / 879
LOX3_BRADI / 946
LOX4_BRADI / 837
LOX5_BRADI / 894
LOX6_BRADIch / 779
LOX7_BRADIch / 900
LOX8_BRADIch / 935
LOX9_BRADI / 864
LOX10_BRADIch / 954
Barley (Hordeum vulgare) / LOX1_HORVU (LOX-1) / P29114 / 862
Q42846_HORVU / Q42846 / 876
LOX21_HORVUch / P93184 / 936
LOX22_HORVUch / Q8GSM3 / 932
LOX23_HORVUch / Q8GSM2 / 896
Q42847_HORVD (LOX-2) / Q42847 / 864
Q53HZ3_HORVDch / Q53HZ3 / 864
Maize (Zea mays) / Q9LKL4_MAIZE / Q9LKL4 / 873
A1XCI2_MAIZEch / A1XCI2 / 921
A1XCI3_MAIZEch / A1XCI3 / 941
Q06XS3_MAIZEch / Q06XS3 / 905
A1XCI6_MAIZEch / A1XCI6 / 911
A1XCI5_MAIZEch / A1XCI5 / 905
Q06XS2_MAIZEch / Q06XS2 / 911
A1XCI4_MAIZEch / A1XCI4 / 922
A1XCH8_MAIZE / A1XCH8 / 871
A1XCH9_MAIZE / A1XCH9 / 877
Q9AXG8_MAIZE / Q9AXG8 / 864
A1XCI0_MAIZE / A1XCI0 / 887
Q8W0V2_MAIZE / Q8W0V2 / 864
B6U297_MAIZE / B6U297 / 873
A1XCI7_MAIZE / A1XCI7 / 850
A1XCI1_MAIZE / A1XCI1 / 892
Common wheat (Triticum aestivum) / TaLOX1 (LOX1_WHEAT) / C6K7G4 / 861
TaLOX2 (LOX2_WHEAT) / C6K7G3 / 864
TaLOX3 (LOX3_WHEAT) / 878

a The searches for LOX protein sequences reported here were conducted in the protein database UniProt using the key world “lipoxygenase”. LOX proteins were checked for the elements important for activity, and LOXs without these elements were not used in this study. The majority of the LOXs is referred by their Swiss-Prot entry names. Owing to the lack of Swiss-Prot entry names, the LOXs from B. distachyon are tentatively named for the purpose of this work. The common wheat LOX reported in this work are tentatively named as TaLOX3. They are also referred as LOX3_WHEAT, to facilitate phylogenetic analysis. The UniProt accession numbers are provided for those LOXs with Swiss-Prot entry names. Also given are the numbers of amino acid residues in the deduced proteins of the all the LOXs.

b LOX labeled with superscript “ch” indicates the presence of a predicted chloroplast targeting peptide. LOX1_HORVU and Q42847_HORVD represent the two barley seeds LOXs, LOX-1 and LOX-2, respectively (van Mechelen et al., 1999). The chromosomal loci of the LOXs from rice are provided in brackets.