Supplemental Instruction
Iowa State University / Leader: / Shanna
Course: / BIOL 212
Instructor: / Kukday
Date: / 03/03/14
DNA helicase / Single-strand binding protein / Topoisomerase
DNA primase / DNA polymerase I / DNA ligase
Leading strand / Lagging strand / Okazaki fragments
DNA Polymerase III
Separates double-stranded DNA into single strands
Fragments created for segments of the lagging strand
Covalently attaches adjacent okazaki fragments in the lagging strand
Synthesizes short RNA primers
Removes tightened coils ahead of the replication fork
Strands that copy from the 3’-5’
Synthesizes DNA in the leading and lagging strand, removes RNA primers, and fills in gaps
Binds to single-stranded DNA and prevents it from re-forming a double helix
1060 Hixson-Lied Student Success Center v 515-294-6624 v v http://www.si.iastate.edu
Strand that copies from the 5’-3’Removes primers
1. The purine bases in DNA are—
a. Cytosine, thymine and uracil
b. Adenine and guanine
c. Cytosine and thymine
d. Thymine, guanine and cytosine
e. Adenine, uracil and guanine
2. Nitrogen comes in a common light form (14N) and a rare heavy form (15N). In 1958,
Matthew Meselson and Franklin Stahl grew E.coli in medium with only 15N and then
switched to medium with only 14N. They collected samples after each generation,
isolated DNA, and subjected it to centrifugation to differentiate between DNA labeled
with the common light form (14N) and the rare heavy form (15N). Original parental
strands would contain 15N while newly made strands would contain 14N.
How many bands of DNA would you expect to see in the cesium gradient above
after 3 generations if the mechanism of DNA replication was semi-conservative?
a. 1 band that is half heavy
b. 2 bands, one is light and the other half heavy
c. 3 bands, one is heavy, one is half-heavy, and one is light
d. 2 bands, one is heavy and one is light
3. A drug that increases the number of NADH & FADH2 molecules produced
through the citric acid cycle/Krebs cycle and has no other effects on
the cell would theoretically
a. decrease the activity of ATP synthase.
b. decrease the cell’s ability to manufacture glycerol.
c. increase the number of ATPs produced per glucose.
d. increase lactic acid production.
e. decrease the cell’s need for oxygen.
4. Photosystem II strips electrons from chlorophyll and delivers them to
the chloroplast electron transport (cytochrome) chain. Where do the
replacement electrons come from?
a. splitting O2
b. photosystem I
c. splitting H2O
d. splitting CO2
e. NADPH
5. A reaction will proceed spontaneously when -
a. it is endergonic.
b. It is exergonic
6. In the following graph, the change in free energy is represented by –
a. A + C or B + C
b. A or B
c. C
d. C – A or B
7. In the following graph, the same chemical reaction is represented. The different
curves either have no inhibitor present, a non-competitive inhibitor, or a competitive
inhibitor. Which curve represents when a non-competitive inhibitor is present?
a. A
b. B
c. C
8. What is the function of topoisomerase during DNA replication?
a. It separates the two strands of the double helix.
b. It relieves the overwinding of DNA ahead of the replication fork due to
DNA strand separation.
c. It synthesizes the RNA primers used to initiate DNA replication.
d. It joins the Okazaki fragments together.
9. Which of the following statements about DNA replication is NOT correct?
a. Leading strand synthesis is continuous, while lagging strand synthesis is
discontinuous.
b. Both leading and lagging strands are synthesized in the 5’ to 3’ direction.
c. Leading strand synthesis is conservative, while lagging strand synthesis
is semi-conservative.
d. Completion of the lagging strand requires ligation of Okazaki fragments
10. Why did the experimenters treat sample E with Protease?
a. To activate the DNA
b. To allow the DNA to enter the R strain bacteria.
c. To purify the DNA from other materials in the extract.
d. To demonstrate that protein is not the genetic material.
e. All of the above
11. In this very simple diagram of a DNA replication fork shown above, which red
strand(s) is the leading DNA strand?
a. A
b. B
c. A and B
d. Neither A nor B
12. Where is the chlorophyll located in a plant?
a. Cristae
b. Plasma membrane of the cell
c. Outer membrane of the chloroplast
d. Thylakoid membrane
e. Stroma
13. Which of the following is not considered a common level of gene regulation in prokaryotes?
a. transcriptional
b. RNA processing
c. translational
d. post-translational
e. All of the above are levels at which prokaryotes are able to
regulate gene expression
14. Which of the following best represents the central dogma of gene expression?
a. During transcription, DNA codes for polypeptides.
b. During transcription, DNA codes for mRNA, which codes for polypeptides during translation.
c. During translation, DNA codes for mRNA, which codes for polypeptides during transcription.
d. none of the above
15. A DNA strand in double-stranded DNA is based paired with its complementary strand. The strand complementary to a strand with the sequence 5’-GCAAGTCATGCCGAAT-3’ would read as follows:
a. 5’-CGTTCAGTACGGCTTA-3’
b. 5’-ATTCGGCATGACTTGC-3’
c. 5’- GCAAGTCATGCCGAAT-3’
d. 5’-ATGCAGTTACAATGCA-3
16. What supplies the electrons to the calvin cycle?
a. water
b. oxygen
c. electron transport from photosystem 1
d. NADPH
e. ATP
17. Stomata are sites of...
a. light reactions
b. calvin cycle
c. gas exchange
d. electron transport chain
e. carbon fixation
- 1.5 g of radish seeds in each of three dishes. The following conditions are created for each dish and incubated at room temperature:
The dry biomass of three treatments at the end of the experiment is shown above.
In the presence of no light with water the seedlings have decreased biomass because
a. photosynthesis does not occur.
b. plants use energy stored the seeds to sprout.
c. the release of CO2 into the air during respiration.
d. the incorporation of CO2 into carbohydrates during the Calvin cycle does not occur.
e. all of the above
19. A drug is designed to make the glycolytic pathway more productive. What part of the cell would the drug need to be delivered to for it to directly affect this pathway?
a. mitochondrial matrix
b. cytosol/cytoplasm
c. mitochondrial intermembrane space
d. nucleus
e. chloroplast
20. What are the common features of a promoter?
a. TATA box
b. Regulator
c. transcriptional start site
d. regulatory elements
e. A, C, D
f. All of the above
21. What is the purpose of gene regulation?
a. Make mRNA
b. Make proteins
c. Ensure that the cells make perfect protein
d. Create different type of cells within a multicellular organism
22. The functional product of a structural gene is
a. tRNA.
b. mRNA.
c. rRNA.
d. a polypeptide.
e. a, b, and c
23.
If the above reactions are coupled they will have a net ΔG of:
a. +4kcal/mol endergonic
b. -4kcal/mol exergonic
c. +4kcal/mol exergonic
d. -4kcal/mol endergonic
24. T/F the promoter region of a gene is transcribed
25. T/F One gene encodes one protein
26. What is true about the following graph?
a. It is exergonic & will occur spontaneously
b. It is exergonic & will not occur spontaneously
c. It is endergonic & will occur spontaneously
d. It is endergonic & will not occur spontaneously
27. The ATP produced during glycolysis and Kreb’s Cycle are synthesized via
a. Allosteric regulation
b. Substrate-level phosphorylation
c. Oxidative phosphorylation
d. ATP synthase
e. Phosphofructokinase
28. Glycolysis produces a net energy gain of ______from one glucose molecule.
A. 2 NADH, 2 ATP, 2 Pyruvate
b. 1 NADH, 1 ATP, 1 Pyruvate
c. 2 NADH, 2 ATP, 2 Citrate
d. 1 NADH, 1 ATP, 1 Citrate
e. None of the above
29. Leading strand synthesis is ______and lagging strand synthesis is ______.
a. discontinuous:continuous
b. conservative:semiconservative
c. semiconservative:conservative
d. rapid:slow
e. continuous:discontinuous
30. which of the following applies to both transciption and translation
a. promoter and terminator
b. intiation complex and open complex
c. activation, regulation and termination
d. initiation, elongation and termination
e. activation, processing and termination