“Development and validation of a multiplex reaction analyzing 8 miniSTRs of the X chromosome for identity and kinship testing with degraded DNA”, International Journal of Legal Medicine, María Castañeda, Adrián Odriozola¹, Javier Gómez², María T. Zarrabeitia

Corresponding author: María Teresa Zarrabeitia, Unit of Legal Medicine, University of Cantabria, Av Herrera Oria s/n, Santander 39011, Cantabria, Spain.

Supplementary Table S5a.Haplotypes frequencies on ChrX linkage group DXS7132-DXS10079-DXS10074-DXS10075 (N=22 males from Pas valley).

DXS7132 / DXS10079 / DXS10074 / DXS10075 / Number / Frequency
H1 / 12 / 19 / 19 / 18 / 2 / 0.09091
H2 / 13 / 15 / 17 / 16 / 1 / 0.04545
H3 / 13 / 18 / 19 / 18 / 1 / 0.04545
H4 / 13 / 19 / 17 / 17 / 1 / 0.04545
H5 / 13 / 20 / 16 / 17 / 1 / 0.04545
H6 / 13 / 20 / 17 / 17 / 1 / 0.04545
H7 / 13 / 20 / 18 / 17 / 1 / 0.04545
H8 / 13 / 21 / 18 / 14 / 1 / 0.04545
H9 / 13 / 21 / 18 / 18 / 1 / 0.04545
H10 / 13 / 21 / 20 / 17 / 1 / 0.04545
H11 / 13 / 22 / 13 / 17 / 1 / 0.04545
H12 / 13 / 22 / 18 / 18 / 1 / 0.04545
H13 / 13 / 23 / 13 / 17 / 1 / 0.04545
H14 / 14 / 17 / 16 / 16 / 1 / 0.04545
H15 / 14 / 20 / 7 / 13 / 1 / 0.04545
H16 / 14 / 20 / 15 / 17 / 1 / 0.04545
H17 / 14 / 20 / 18 / 17 / 1 / 0.04545
H18 / 15 / 20 / 19 / 17 / 1 / 0.04545
H19 / 15 / 22 / 14 / 17 / 1 / 0.04545
H20 / 16 / 20 / 16 / 18 / 1 / 0.04545
H21 / 16 / 22 / 14 / 17 / 1 / 0.04545

Supplementary Table S5b. Haplotypes frequencies on ChrX linkage group DXS7132-DXS10079-DXS10074-DXS10075 (N=45 females from Pas Valley).

DXS7132 / DXS10079 / DXS10074 / DXS10075 / Number / Frequency
H1 / 15 / 20 / 19 / 17 / 3 / 0.06667
H2 / 12 / 19 / 19 / 18 / 2 / 0.04444
H3 / 12 / 21 / 17 / 18 / 2 / 0.04444
H4 / 12 / 22 / 16 / 18 / 2 / 0.04444
H5 / 13 / 19 / 17 / 17 / 2 / 0.04444
H6 / 13 / 20 / 17 / 17 / 2 / 0.04444
H7 / 13 / 21 / 18 / 18 / 2 / 0.04444
H8 / 13 / 22 / 13 / 17 / 2 / 0.04444
H9 / 14 / 20 / 7 / 13 / 2 / 0.04444
H10 / 14 / 20 / 18 / 17 / 2 / 0.04444
H11 / 12 / 20 / 17 / 17 / 1 / 0.02222
H12 / 12 / 21 / 17 / 17 / 1 / 0.02222
H13 / 12 / 22 / 17 / 17 / 1 / 0.02222
H14 / 13 / 15 / 17 / 16 / 1 / 0.02222
H15 / 13 / 18 / 19 / 18 / 1 / 0.02222
H16 / 13 / 20 / 15 / 18 / 1 / 0.02222
H17 / 13 / 20 / 16 / 17 / 1 / 0.02222
H18 / 13 / 20 / 18 / 17 / 1 / 0.02222
H19 / 13 / 21 / 18 / 14 / 1 / 0.02222
H20 / 13 / 21 / 20 / 17 / 1 / 0.02222
H21 / 13 / 22 / 18 / 18 / 1 / 0.02222
H22 / 13 / 23 / 13 / 17 / 1 / 0.02222
H23 / 13 / 24 / 19 / 17 / 1 / 0.02222
H24 / 14 / 17 / 16 / 16 / 1 / 0.02222
H25 / 14 / 19 / 16 / 18 / 1 / 0.02222
H26 / 14 / 19 / 18 / 17 / 1 / 0.02222
H27 / 14 / 20 / 15 / 17 / 1 / 0.02222
H28 / 14 / 21 / 17 / 17 / 1 / 0.02222
H29 / 14 / 22 / 19 / 17 / 1 / 0.02222
H30 / 15 / 17 / 19 / 17 / 1 / 0.02222
H31 / 15 / 22 / 14 / 17 / 1 / 0.02222
H32 / 16 / 20 / 8 / 17 / 1 / 0.02222
H33 / 16 / 20 / 16 / 18 / 1 / 0.02222
H34 / 16 / 22 / 14 / 17 / 1 / 0.02222

Supplementary Table S5c. Haplotypes frequencies on ChrX linkage group DXS6801-6809-6789-6799 (N=22 males from Pas Valley).

DXS6801 / DXS6809 / DXS6789 / DXS6799 / Number / Frequency
H1 / 11 / 35 / 20 / 11 / 3 / 0.13636
H2 / 12 / 33 / 20 / 12 / 3 / 0.13636
H3 / 12 / 35 / 20 / 12 / 2 / 0.09091
H4 / 11 / 30 / 21 / 11 / 1 / 0.04545
H5 / 11 / 30 / 21 / 12 / 1 / 0.04545
H6 / 11 / 31 / 20 / 12 / 1 / 0.04545
H7 / 11 / 31 / 22 / 12 / 1 / 0.04545
H8 / 11 / 32 / 21 / 12 / 1 / 0.04545
H9 / 11 / 33 / 20 / 13 / 1 / 0.04545
H10 / 11 / 33 / 21 / 10 / 1 / 0.04545
H11 / 11 / 34 / 21 / 12 / 1 / 0.04545
H12 / 11 / 35 / 21 / 11 / 1 / 0.04545
H13 / 12 / 30 / 15 / 12 / 1 / 0.04545
H14 / 12 / 34 / 15 / 11 / 1 / 0.04545
H15 / 12 / 35 / 24 / 12 / 1 / 0.04545
H16 / 12 / 35 / 24 / 13 / 1 / 0.04545
H17 / 13 / 34 / 21 / 11 / 1 / 0.04545

Supplementary Table S5d. Haplotypes frequencies on ChrX linkage group DXS6801-6809-6789-6799 (N=45 females from Pas Valley).

DXS6801 / DXS6809 / DXS6789 / DXS6799 / Number / Frequency
H1 / 12 / 33 / 20 / 12 / 5 / 0.11111
H2 / 11 / 34 / 21 / 12 / 4 / 0.08889
H3 / 11 / 35 / 20 / 11 / 3 / 0.06667
H4 / 11 / 32 / 23 / 13 / 2 / 0.04444
H5 / 12 / 35 / 20 / 12 / 2 / 0.04444
H6 / 10 / 31 / 22 / 12 / 1 / 0.02222
H7 / 10 / 33 / 20 / 11 / 1 / 0.02222
H8 / 10 / 33 / 20 / 13 / 1 / 0.02222
H9 / 11 / 24 / 20 / 10 / 1 / 0.02222
H10 / 11 / 30 / 21 / 11 / 1 / 0.02222
H11 / 11 / 30 / 21 / 12 / 1 / 0.02222
H12 / 11 / 31 / 20 / 12 / 1 / 0.02222
H13 / 11 / 31 / 22 / 11 / 1 / 0.02222
H14 / 11 / 31 / 22 / 12 / 1 / 0.02222
H15 / 11 / 32 / 21 / 12 / 1 / 0.02222
H16 / 11 / 33 / 20 / 13 / 1 / 0.02222
H17 / 11 / 33 / 21 / 10 / 1 / 0.02222
H18 / 11 / 33 / 21 / 11 / 1 / 0.02222
H19 / 11 / 33 / 21 / 12 / 1 / 0.02222
H20 / 11 / 34 / 21 / 10 / 1 / 0.02222
H21 / 11 / 35 / 21 / 11 / 1 / 0.02222
H22 / 11 / 35 / 23 / 12 / 1 / 0.02222
H23 / 12 / 30 / 15 / 12 / 1 / 0.02222
H24 / 12 / 31 / 20 / 11 / 1 / 0.02222
H25 / 12 / 32 / 20 / 11 / 1 / 0.02222
H26 / 12 / 33 / 21 / 12 / 1 / 0.02222
H27 / 12 / 34 / 15 / 11 / 1 / 0.02222
H28 / 12 / 34 / 20 / 11 / 1 / 0.02222
H29 / 12 / 34 / 21 / 11 / 1 / 0.02222
H30 / 12 / 34 / 21 / 12 / 1 / 0.02222
H31 / 12 / 35 / 24 / 12 / 1 / 0.02222
H32 / 12 / 35 / 24 / 13 / 1 / 0.02222
H33 / 13 / 34 / 21 / 11 / 1 / 0.02222
H34 / 13 / 34 / 21 / 12 / 1 / 0.02222