Lendvaiet al., 2012

SupplementaryTable 2 – List of markers used for immunofluorescence labelling

Marker / Source / Host / IH dilution / WB dilution / Reference
Anti-human CRTL-1 (hHAPLN1) / RD Systems Inc. / Goat, pc2 / 1:200 / 1:400 / Carulli et al. ( 2007)
Anti-aggrecan HAG7D4 / Acris / Mouse, mc1 / 1:10 / 1:50 / Brückner et al (2008)
Anti-aggrecan AB1031 / Millipore / Rabbit, pc2 / 1:1,000 / 1:2,000 / Giamanco et al (2010)
Brevican / Santa Cruz / Goat, pc2 / n.a. / 1:500 / this report
Brevican (50kDa fragment) / R. T. Matthews / Rabbit, pc2 / 1:2,000 / n.a. / Matthews et al (2000)
Cat-301 (MAB1581) / Millipore / Mouse, mc1 / 1:1,000 / 1:1,000 / Matthews et al (2002)
Wisteria floribunda agglutinin / Sigma / lectin / 25 µg/ml / n.a. / Morawskiet al (2011)

β-Actin

/ Sigma / Mouse, mc1 / n.a. / 1:10,000 / Bouchard et al (2007)
β-Amyloid / Millipore / Mouse, mc1 / 1:200 / n.a. / Thal et al (1999)
Calbindin / SWant / Mouse, mc1 / 1:2,000 / n.a. / Celio et al (1990)

Calretinin

/ SWant / Goat, pc2 / 1:2,000 / n.a. / Schwaller et al (2003)
Glutamate decarboxylase (GAD65/67) / Sigma / Rabbit, pc2 / 1:5,000 / n.a. / Brückner et al (2008)
MAP2 / Sigma / Mouse, mc1 / 1:200 / n.a. / Binder et al (1996)

Parvalbumin

/ SWant / Rabbit, pc2 / 1:5,000 / n.a. / Celio et al (1990)
PHF tau / Pierce / Mouse, mc1 (AT8) / 1:1,000 / n.a. / Braak et al (1994)
Vesicular GABA transporter (VGAT) / Synaptic Systems / Rabbit, pc2 / 1:500 / n.a. / Hartig et al (2003)
Vesicular glutamate transporter type 1 (VGLUT1) / Synaptic Systems / Rabbit, pc2 / 1:5,000 / n.a. / Kaneko et al (2002)

1monoclonal antibody

2polyclonal antibody