Siobhan M. Deis

735 S. Lafayette Dr., Apt. 230

Lafayette, CO 80026

317-331-6183

EDUCATION

Ph.D., Interdisciplinary Graduate Program in Biochemistry

Indiana University, Bloomington, IN, June 2016

Bachelor of Arts in Biochemistry

DePauw University, Greencastle, IN, May 2011

Cum Laude

RESEARCH EXPERIENCE

Graduate Research Assistant, Department of Chemistry

Indiana University, Bloomington, INJanuary 2012-June 2016

  • Designed and executed structural and biochemical analyses of enzyme inhibition and folate receptor specific transportfor a set of novel antifolate drugsto drive informed, hypothesis-based drug design
  • Mentored four undergraduate students to be independent scientists

Science Research Fellow,

DePauw University, Greencastle, IN 2009-2010

  • Synthesizedmetallo-organic networks in ionic liquids and characterizedthe products
  • Trained fellow undergraduates in lab techniques and experimental design

LAB/TECHNICAL SKILLS

  • Macromolecular x-ray crystallography including crystal growth using automation robotics, crystal freezing, data-collection, data processing, phasing, and structure refinement of protein-ligand complexes
  • Characterization of receptor-ligand binding interactions via isothermal titration calorimetry (ITC), stopped-flow, surface-plasmon resonance (SPR), and microscale thermophoresis (MST)
  • Measurement of small-molecule enzyme inhibition via a spectrophotometric assay
  • Expression of human proteins in bacteria, stable mammalian cell lines, and a transient BacMam system
  • Protein purification via affinity and size-exclusion chromatography (FPLC)
  • Western blotting
  • Cloning of recombinant genes for protein expression

PUBLICATIONS

Wang, L., Wallace, A., Raghavan, S., Deis, S.M.,et al. 6-Substituted Pyrrolo[2,3-d]pyrimidine Thienoyl Regioisomers as Targeted Antifolates for Folate Receptor α and the Proton-coupled Folate Transporter in Human Tumors. Journal of Medicinal Chemistry. 2015.

Deis, S.M., et al.Structural and enzymatic analysis of glycinamide ribonucleotide formyltransferase inhibitors that transport specifically via human folate receptors. In review.

Golani, L.K., Wallace-Povirk, A., Deis, S.M., et al.Tumor Targeting with Novel 6-Substituted Pyrrolo [2,3-d] Pyrimidine Antifolates with Heteroatom Bridge Substitutions Via Cellular Uptake by Folate Receptor α and the Proton-coupled Folate Transporter and Inhibition of De Novo Purine Nucleotide Biosynthesis. In review.

PRESENTATIONS

Presented research entitled “Analysis of Antifolate Drugs with Disease Tissue Specificity” at the following venues:

  • Indiana Clinical and Translational Sciences Institute Structural Biology Forum, Indianapolis, IN, 2016
  • Indiana University Chemistry Career Development Symposium, Bloomington, IN, 2015
  • Biophysical Society Annual Meeting, Baltimore, MD, 2015
  • BMB Seminar Series, Bloomington, IN, 2015
  • Genetics, Cellular, and Molecular Sciences Training Grant Symposium, Bloomington, IN, 2014, 2013
  • Watanabe Symposium in Chemical Biotechnology, Bloomington, IN 2014, 2013
  • Mid-Atlantic Graduate Student Symposium in Medicinal Chemistry,Columbus, OH, 2013
  • FASEB: Folic Acid, Vitamin B12 and One Carbon Metabolism, Crete,Greece, 2012

HONORS

Felix Haurowitz Award, Indiana University, 2014

Mid-Atlantic Graduate Student Symposium in Medicinal Chemistry Oral Presentation Award: 3rd place, 2013

NIH Genetics, Cellular and Molecular Sciences (GCMS) Training Grant, Indiana University, 2012-2014

Attended the Cold Spring Harbor Laboratory course on X-Ray Methods in Structural Biology, Cold Spring Harbor, NY, 2012

Indiana University Graduate School Fellowship (merit-based), 2012

SERVICE

Biochemistry Graduate Representative Committee, Co-Founder and President, 2015-present

Graduate Recruitment Weekend Volunteer, Molecular and Cellular Interdisciplinary Biochemistry Program, 2012-2016

Indiana Science Olympiad State Tournament Volunteer, 2015, 2012