OIE RL for « Rabies » – « Changchun Tu » – « China »

OIE Reference Laboratory Reports
Activities in 2012

Name of disease (or topic) for which you are a designated OIE Reference Laboratory: / Rabies
Address of laboratory / Changchun Veterinary Research Institute
Chinese Academy of Agriculture Sciences
666 Liuying West Rd., Jingyue Economic Development Zone, Changchun,
CHINA
Tel.: / +86-431-86985921
Fax: / +86-431-81032022
e-mail address: /
website: / --
Name (including Title) of Head of Laboratory (Responsible Official): / Dr Changchun Tu, Professor
Name(including Title and Position) of OIE Reference Expert: / Dr Changchun Tu, Professor
Date of submission to the OIE / January 22th 2013

ToR:To use, promote and disseminate diagnostic methods validated according to OIE Standards

To investigate and analyze the status and characteristics of animal rabies, surveillance measures including:

(i) Surveillance on apparently healthy animals, (ii)Laboratory confirmation of suspected animals, (iii) Wildlife (mainly bats) rabies surveillance, (iv) Investigation on immunization coverage of the domestic dogs in rural areas.

Test recommended by the OIE / Total number of test performed last year
Indirect diagnostic tests / Nationally / Internationally
Fluorescent antibody virus neutralisation (FAVN)* / 257 / 2
Immunochromatographic test strip (ICTS) / 500
Direct diagnostic tests / Nationally / Internationally
Fluorescent antibody test (FAT) / 14 (suspected dogs) / 10** (suspected animals，including dog,goat, buffalo and alpaca)
Cell culture inoculation test (RTCIT) / Above 14 (suspected dogs) / Above 10 suspected animals
Reverse transcription-PCR (RT-PCR) *** / 442 (apparently healthy dog samplesfor pathogen surveillance, including 300 brain tissues and 142 saliva swabs)
2225 bat brain tissues (wildlife surveillance) / --
Real time TaqManRT-PCR(FQ-PCR)*** / 442 (apparently healthy dog samplesfor pathogen surveillance, including 300 brain tissues and 142 saliva swabs)
2225 bat brain tissues (wildlife surveillance) / --
G gene and N gene Sequencing / -- / Above 10 suspected animals

*FAVN was carried out to assess vaccinationefficacy ofrural and companiondogs.

**10 suspected animal samples were from Nepal.

*** RT-PCR and FQ-PCR isused to screen large number of the samples collected from apparently healthy dogs. PCR positive brain tissues are further confirmed by FAT. All the brain samples of apparently healthy dogs were collected from the restaurants selling dog meat. Saliva samples were collected from apparently healthy rural dog in rabies endemic areas.

ToR:To develop reference material in accordance with OIE requirements, and implement and promote the application of OIE Standards.

Tostore and distribute to national laboratories biological reference products and any other reagents used in the diagnosis and control of the designated pathogens or disease.

2.Did your laboratory produce or store imported standard reference reagentsofficially recognised by the OIE or other international bodies?

YesNo

3.Did your laboratory supply standard reference reagentsto OIE Member Countries?

YesNo

Type of reagent available / Related diagnostic test / Produced/
stored / Amount supplied nationally
(ml, mg) / Amount supplied internationally
(ml, mg) / Name of recipient OIE Member Countries and of institutions
Fluorescent antibody conjugate / FAT / Stored / 5ml / Rabies Vaccine Production Laboratory of Nepal
Newborncalf serum / RTCIT / Stored / 200 ml / Rabies Vaccine Production Laboratory of Nepal

4.Did your laboratory produce diagnostic reagents other than the OIE-approved standard reference reagents?

YesNo

  • Rabies virus RT-PCR kit and Real time TaqManRT-PCR kit were produced and donated to AnimalCenter for Disease Control and Prevention of BananDistrict of Chongqing Municipality, China, in order to promote the application of rabies diagnosis at county level.

5.Did your laboratory produce vaccines?

YesNo

6.Did your laboratory supply vaccines to OIE Member Countries?

YesNo

ToR:To develop, standardise and validate, according to OIE Standards, new procedures for diagnosis and control of the designated pathogens or diseases

7.Did your laboratory develop new diagnostic methods validated according to OIE Standards for the designated pathogen or disease?

Yes No

8.Didyour laboratory develop new vaccines according to OIE Standards for the designated pathogen or disease?

YesNo

Name of the new test or diagnostic method or vaccine developed / Description and References (Publication, website, etc.)
Real time TaqManRT-PCR / YunbinXu, et al. (2012). Evaluation and application of one step real-time TaqMan RT-PCR kit for detection of classic rabies virus, Chin J Vet Sci,32(3):420-26.
YunbinXu, et al. (2011).Establishment of the one-step real--time RT-PCRassay for detection of genotype 1 lyssaviru，Chin J Zoonoses，27(4)：297-302.
Oligonucleotide microarray, LyssaChip / Jin Xi, Huancheng Guo, Changchun Tu, et al. (2012). Differentiation of the seven major lyssavirus species by oligonucleotide microarray, J ClinMicrobiol, 50(3): 619-25
Rapid immunochromatographic test strip (ICTS) / Wang et al. (2010).A rapid immunochromatographic test strip for detecting rabies virus antibody. Journal of Virological Methods, 170 (2010) 80-85.
Recombinant vaccine (ERA) / Manuscript in preparation

ToR:To provide diagnostic testing facilities, and, where appropriate, scientific and technical advice on disease control measures to OIE Member Countries

9.Did your laboratory carry out diagnostic testing for other OIE Member Countries?

Yes No

Name of OIE Member Country seeking assistance / Date
(dd/mm) / No. samples received for provision of diagnostic support (i.e. from surveillance campaign) / No. samples received for provision of confirmatory diagnoses
Nepal / 15 August / -- / 10

10.Did your laboratory provide expert advice in technical consultancies on the request of an OIE Member Country?

Yes No

Name of the OIE Member Country receiving a technical consultancy / Purpose / How the advice was provided
Rabies Vaccine Production Laboratory of Nepal / Monitor epidemicsituationof rabies in the country / Meeting discussion

ToR:To carry out and/or coordinate scientific and technical studies in collaboration with other laboratories, centres or organisations

11.Did your laboratory participate in international scientific studies in collaboration with OIE Member Countries other than the own?

YesNo

ToR:To collect, process, analyse, publish and disseminate epizootiological data relevant to the designated pathogens or diseases

12.Didyour Laboratory collect epizootiologicaldata relevant to international disease control?

YesNo

13.Didyour laboratory disseminate epizootiologicaldata that had been processed and analysed?

Yes No

14.What method of dissemination of information is most often used by your laboratory?

(Indicate in the appropriate box the number by category)

a)Articles published in peer-reviewed journals:...... 5

  • Jin Xi, Huancheng Guo, Changchun Tu, et al. (2012). Differentiation of the seven major lyssavirus species by oligonucleotide microarray, J ClinMicrobiol, 50(3): 619-25.
  • Yunbin Xu, et al. (2012). Evaluation and application of one step real-time TaqMan RT-PCR kit for detection of classic rabies virus, Chin J Vet Sci,32(3):420-26.
  • PingsenZhao, Hualei Wang, Songtao Yang, et al. (2012) Changes in microRNA expression induced by rabies virus infection in mouse brains. MicrobPathog 52: 47-54.
  • PingsenZhao, Hualei Wang, Songtao Yang, et al. (2012) Infection with street strain rabies virus induces modulation of the microRNA profile of the mouse brain. Virol J 9: 159.
  • HongruLiang, Hualei Wang, Songtao Yang, et al. (2012) Isolation of ssDNAaptamers that inhibit rabies virus. IntImmunopharmacol 14: 341-347.

b)International conferences:...... 4

  • Changchun Tu. Laboratory diagnosis of rabies. Regional Animal Health Laboratory Technical Advisory Group Meeting. 30-31 July2012. Bangkok, Thailand
  • Changchun Tu. Rabies in China. Compendium of the OIE Global Conference on Rabies Control. 7-9th Sepertamber 2011, Incheon-Seoul, Kora.
  • Ye Feng.Rabies control demonstration in Bana District, Chongqing City,United Nations Theme Group on Health (UNTGH)4thSub-working Group on Diseases at the Human-Animal InterfaceMeeting.14th September 2012, Beijing, China.
  • Hualei Wang, Songtao Yang. Serological survey of parvovirus in carnivores, China, WDA-EWDA conference, 22-27th July 2012, Lyon, France

c)National conferences:...... 5

  • Changchun Tu, et al. Animal rabies surveillance in China. 2012 Rabies Conference in China, 17-18th May 2012, Beijing, China.
  • Jiang Ma,et al.Virus shedding and tissue distribution following challenge of dogs with two strains of street rabies viruses, 2012 Rabies Conference in China, 17-18th May 2012, Beijing, China.
  • Yan Zhu, et al.An outbreak of sheep rabies in Shanxi province, China, 2012 Rabies Conference in China, 17-18th May 2012, Beijing, China.
  • Songtao Yang, Hualei Wang, XianzhuXia, et al. (2012) Study on comprehensive prevention and control of rabies. 2012 Rabies Conference in China, 17-18th May 2012, Beijing, China.
  • Hualei Wang, Songtao Yang, XianzhuXia,et al. (2012) Recombinant rabies viruses expressing chemokines/cytokines can be used effectivelyto prevent the development of rabies in a mouse model. 2012 Rabies Conference in China, 17-18th May 2012, Beijing, China.

d)Other:...... 2

Report of rabies work in 2012

  • The annual report of our laboratory submitted to Chinese Ministry of Agriculture.
  • Summary on animal rabies control programme in Banan district of Chongqing Municipalitysubmitted to Chinese Ministry of Agriculture.

ToR: To provide scientific and technical training for personnel from OIE Member Countries

To recommend the prescribed and alternative tests or vaccines as OIE Standards

15.Did your laboratory provide scientific and technical training to laboratory personnel from other OIE Member Countries?

Yes No

a)Technical visits:...... 1

b)Seminars:...... --

c)Hands-on training courses:...... --

d)Internships (>1 month):...... --

Type of technical training provided (a, b,c or d) / Country of origin of the expert(s) provided with training / No. participants from the corresponding country
a / Nepal / 1

ToR:To maintain a system of quality assurance, biosafety and biosecurity relevant for the pathogen and the disease concerned

16.Does your laboratory have a Quality Management System certified according to an International Standard?

Yes No

Quality management system adopted
ISO 17025

17.Is your laboratory accredited by an international accreditation body?

Yes No

18.Does your laboratory maintain a “biorisk management system” for the pathogen and the disease concerned? (See Manual of Diagnostic Tests and Vaccines for Terrestrial Animals 2012, Chapter 1.1.3 orManual of Diagnostic Tests for Aquatic Animals 2012, Chapter 1.1.1)

Yes No

ToR:To organise and participate in scientific meetings on behalf of the OIE

19.Did your laboratory organise scientific meetings on behalf of the OIE?

Yes No

20.Did your laboratory participate in scientific meetings on behalf of the OIE?

YesNo

Title of event / Date
(mm/yy) / Location / Role (speaker, presenting poster, short communications) / Title of the work presented
Regional Animal Health Laboratory Technical Advisory Group Meeting / 30-31 July / Bangkok, Thailand / Speaker / Laboratory diagnosis of rabies

ToR:To establish and maintain a network with other OIE Reference Laboratories designated for the same pathogen or disease and organise regular inter-laboratory proficiency testing to ensure comparability of results

21.Did your laboratory exchange information with other OIE Reference Laboratories designated for the same pathogen or disease?

Yes No

22.Was your laboratory involved in maintaining a network with OIE Reference Laboratories designated for the same pathogen or disease by organising or participating in proficiency tests?

Yes No

Purpose of the proficiency tests: (validation of a diagnostic protocol: specify the test; quality control of vaccines: specify the vaccine type, etc.) / Role of your Reference Laboratory (organiser/participant) / No. participants / Participating OIE Ref. Labs/ organising OIE Ref. Lab.
Seroneutralisation Proficiency Test / Participant / 2 / European Union Reference Laboratory ANSES (Organiser)

23.Did your laboratory collaborate with other OIE Reference Laboratories for the same disease on scientific research projects for the diagnosis or control of the pathogen of interest?

Yes No

ToR:To organise inter-laboratory proficiency testing with laboratories other than OIE Reference Laboratories for the same pathogens and diseases to ensure equivalence of results.

24.Did your laboratory organiseor participate in inter-laboratory proficiency tests with laboratories other than OIE Reference Laboratories for the same disease?

YesNo

ToR:To place expert consultants at the disposal of the OIE

25.Did your laboratory place expert consultants at the disposal of the OIE?

Yes No

Kind of consultancy / Location / Subject (facultative )
OIE ad hocGroup Meeting / Paris / Update and revision of Section C (Requirements for Vaccines) of Chapter 2.1.13 Rabies of the Terrestrial Manual

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Annual reports of OIE Reference Laboratories, 20121