Supplementary Document

Supplementary document

Spatio-temporal distribution of fecal indicators in three rivers of the Haihe River Basin, China

Yawei Wang1, Yanan Chen1, 2, Xiang Zheng2，*, Chengmin Gui1, Yuansong Wei1*,

1 State Key Joint Laboratory of Environmental Simulation and Pollution Control, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China

2 School of Environment and Natural Resources, Renmin University of China, Beijing 100872, China

*Corresponding author:Tel: +86-10-62849690,Fax: +86-10-62849690,E-mail address: (Y. Wei), (zhengxiang)

Table S1 water quality criteria in China (GB 3838-2002)

Grade1 / COD(≤mg/L) / NH3-N(≤mg/L) / FC(≤ CFU/L) / Designated Uses
I / 15 / 0.15 / 200 / Water sources and national nature reserves
II / 15 / 0.5 / 2000 / Primary reserve zones providing centralized domestic and drinking water; habitats of rare aquatic; Spawning grounds of shrimp; Feeding grounds of larvae larval.
III / 20 / 1.0 / 10000 / Secondary protection zone providing centralized domestic and drinking water; Wintering grounds of shrimp; Migration pathway; Aquaculture, fishing and swimming areas.
IV / 30 / 1.5 / 20000 / General Industrial water zones and water recreation areas where no direct contact with humans occurs.
V / 40 / 2.0 / 40000 / Agricultural water zones and scenic water areas.

I-the best, V-the worst.

Multi-purpose water zones are classified based on their primary function. Seasonal water zones are classified based on a seasonal basis.

Table S2 physical-chemical indexes in four seasons

(a)  December

T / pH / O2 / Conductivity / Turbidity / COD / DIP / NH3-N / NO3-N / Salinity / TDS
°C / mg/L / µS/cm / NTU / mg/L / mgP/L / mgN/L / mgN/L / PSU / ppm
Yang River / YD01 / 1.3 / 8.20 / 12.3 / 566.3 / 0.4 / 20 / 0.45 / 0.64 / 12.34 / 0.17 / 285.92
YD02 / 6.1 / 7.85 / 11.2 / 492.7 / 0.67 / 19 / 0.00 / 1.76 / 9.87 / 0.12 / 249.17
YD03 / 6.5 / 8.25 / 10.3 / 603.6 / 1.29 / 16 / 0.05 / 0.00 / 4.35 / 0.19 / 304.55
YD04 / 4.4 / 8.27 / 12.2 / 876 / 0.41 / 21 / 0.69 / 0.13 / 15.94 / 0.35 / 440.59
YD05 / 5.7 / 8.40 / 10.8 / 1330 / 1.34 / <5 / 0.31 / 0.42 / 9.09 / 0.63 / 667.31
YD06 / 9.2 / 8.45 / 11.1 / 1432 / 0.95 / 25 / 0.04 / 0.45 / 4.31 / 0.69 / 718.25
Wenyu River / WY01 / 4.8 / 8.03 / 8.58 / 833.5 / 2.22 / 31 / 1.06 / 11.94 / 6.70 / 0.33 / 419.36
WY02 / 5.7 / 7.85 / 8.55 / 900.8 / 1.3 / 21 / 1.35 / 15.05 / 5.76 / 0.37 / 452.97
WY03 / 6.2 / 8.25 / 5.8 / 935.9 / 6.1 / 62 / 1.48 / 17.52 / 5.09 / 0.39 / 470.50
WY04 / 11.3 / 7.50 / 0.6 / 1340 / 2.14 / 46 / 1.30 / 16.30 / 3.72 / 0.63 / 672.31
WY05 / 8.1 / 7.49 / 6.34 / 950.6 / 1.37 / 32 / 0.98 / 13.06 / 5.91 / 0.40 / 477.84
WY06 / 10.3 / 7.01 / 5.9 / 966.2 / 0.74 / 36 / 1.59 / 10.75 / 8.34 / 0.41 / 485.63
WY07 / 3.9 / 8.32 / 2.7 / 973.1 / 1.79 / 31 / 1.55 / 17.04 / 6.07 / 0.41 / 489.08
Beiyun River / BY01 / 6.9 / 7.86 / 8.4 / 1080 / 1.82 / 32 / 1.28 / 15.56 / 8.03 / 0.48 / 542.46
BY02 / 7.6 / 8.24 / 11.2 / 1654 / 1.96 / 27 / 0.69 / 4.49 / 12.34 / 0.82 / 829.12
BY03 / 5.7 / 8.29 / 16.8 / 1840 / 1.44 / 22 / 0.03 / 1.80 / 4.70 / 0.93 / 922.01
BY04 / 5.7 / 8.10 / 9.1 / 2176 / 1.54 / 30 / 0.01 / 3.05 / 4.39 / 1.13 / 1089.81
BY05 / 4.4 / 8.41 / 11.3 / 1850 / 1.92 / 26 / 0.18 / 6.61 / 6.23 / 0.94 / 927.00
BY06 / 7.2 / 8.32 / 11.2 / 2150 / 6.59 / 36 / 0.14 / 5.78 / 8.38 / 1.12 / 1076.82
BY07 / 9.3 / 7.87 / 11.7 / 3616 / 0.12 / N.D / 0.01 / 0.61 / 4.27 / 2.00 / 1808.94

(b)  March

T / pH / O2 / Conductivity / Turbidity / COD / DIP / NH3-N / NO3-N / Salinity / TDS
°C / mg/L / µS/cm / NTU / mg/L / mgP/L / mgN/L / mgN/L / PSU / ppm
Yang River / YD01 / 0.8 / 7.92 / 12.22 / 983.2 / 8.29 / 14 / 0.02 / 0.79 / 96.58 / 0.46 / 490.10
YD02 / 0.1 / 7.93 / 12.36 / 1.713 / 9.09 / 11 / N.D / 1.54 / 5.21 / 0.00 / 0.78
YD03 / 0.3 / 8.01 / 14.37 / 1.677 / 18.2 / 22 / N.D / 1.10 / 1.61 / 0.00 / 0.70
YD04 / 2.6 / 7.98 / 12.73 / 1714 / 1.66 / 20 / 0.23 / 1.12 / 10.06 / 1.01 / 855.20
YD05 / 1.8 / 8.05 / 11.97 / 1732 / 16.5 / 11 / 0.01 / 1.07 / 3.53 / 0.30 / 987.30
YD06 / 3.9 / 8.16 / 12.09 / 717.7 / 1.76 / 23 / N.D / 1.19 / 1.42 / 0.35 / 395.40
Wenyu River / WY01 / 6.9 / 7.81 / 8.5 / 534.6 / 10.6 / 38 / 0.30 / 6.55 / N.D / 0.26 / 267.60
WY02 / 9.3 / 7.61 / 5.47 / 543.2 / 5.99 / 27 / 0.35 / 8.25 / 2.86 / 0.27 / 272.10
WY03 / 7.8 / 7.55 / 1.04 / 519.3 / 7.11 / 42 / 0.38 / 6.81 / 0.13 / 0.25 / 260.10
WY04 / 12 / 7.48 / 0.29 / 601.1 / 3.7 / 35 / 0.12 / 7.83 / 4.44 / 0.29 / 301.20
WY05 / 14.2 / 7.84 / 14.05 / 582.2 / 3.37 / 51 / 0.15 / 6.17 / 4.77 / 0.29 / 292.80
WY06 / 10.9 / 7.51 / 1.11 / 598.5 / 3.11 / 39 / 0.03 / 5.94 / 2.49 / 0.29 / 300.00
WY07 / 10.8 / 7.71 / 3.54 / 602.7 / 3.37 / 42 / 0.18 / 6.73 / 3.68 / 0.29 / 301.50
Beiyun River / BY01 / 8.7 / 7.93 / 13.66 / 667.6 / 5.79 / 36 / 0.27 / 8.18 / 4.51 / 0.33 / 334.20
BY02 / 6.9 / 7.93 / 14.38 / 1136 / 6.78 / 36 / 0.13 / 4.80 / 6.05 / 0.57 / 563.60
BY03 / 8.4 / 8.13 / 16.48 / 1054 / 10.3 / 38 / N.D / 1.75 / 0.31 / 0.54 / 496.60
BY04 / 8.8 / 8.07 / 21.21 / 1756 / 8.97 / 49 / 0.03 / 2.60 / 1.24 / 0.63 / 2673.00
BY05 / 9.3 / 7.82 / 15.99 / 1095 / 17.5 / 45 / 0.10 / 6.25 / 8.71 / 0.54 / 545.60
BY06 / 9.4 / 7.74 / 15.19 / 1287 / 36.1 / 51 / 0.07 / 4.91 / 1.18 / 0.65 / 645.50
BY07 / 6.4 / 7.52 / 13.41 / 20360 / 29.8 / N.D / 0.00 / 1.74 / 0.85 / 12.15 / 10170.00

(c)  June

T / pH / O2 / Conductivity / Turbidity / COD / DIP / NH3-N / NO3-N / Salinity / TDS
°C / mg/L / µS/cm / NTU / mg/L / mgP/L / mgN/L / mgN/L / PSU / ppm
Yang River / YD01 / 19.6 / 7.78 / 7.96 / 550.5 / 14.7 / 46 / 0.18 / 0.16 / 12.38 / 0.26 / 275.10
YD02 / 23.3 / 8.62 / 6.84 / 174.1 / 26.2 / 63 / 0.30 / 1.27 / 0.00 / 0.08 / 87.02
YD03 / 26.2 / 7.99 / 7.16 / 597.6 / 73.9 / 24 / 0.06 / 0.30 / 2.95 / 0.28 / 298.80
YD04 / 23.1 / 8.17 / 11.69 / 873.6 / 13 / 6 / 1.12 / 0.68 / 4.25 / 0.42 / 436.60
YD05 / 14.2 / 7.7 / 8.12 / 1036.0 / 107 / 30 / 0.15 / 0.61 / 5.21 / 0.51 / 518.20
YD06 / 27.3 / 8.33 / 11.52 / 1213.0 / 11 / 48 / 0.58 / 0.43 / 0.00 / 0.60 / 606.50
Wenyu River / WY01 / 28.4 / 8.13 / 13.86 / 781 / 16.5 / 56 / 1.69 / 11.70 / 0.05 / 0.38 / 390.40
WY02 / 30.5 / 7.79 / 9.34 / 709.8 / 9.19 / 63 / 1.82 / 13.22 / 0.00 / 0.34 / 354.70
WY03 / 29.8 / 8.07 / 13.79 / 755.4 / 12.3 / 9 / 1.86 / 10.79 / 0.12 / 0.37 / 377.60
WY04 / 28.0 / 7.56 / 0.07 / 844.4 / 86.4 / 27 / 0.27 / 6.38 / 4.67 / 0.41 / 422.40
WY05 / 30.0 / 7.64 / 5.6 / 851.2 / 3.9 / 47 / 2.40 / 9.39 / 0.92 / 0.41 / 425.50
WY06 / 27.1 / 7.5 / 1.05 / 809.5 / 23.4 / 49 / 0.69 / 7.16 / 4.05 / 0.39 / 404.80
WY07 / 29.4 / 7.56 / 2.44 / 875.3 / 6.7 / 45 / 0.41 / 9.39 / 0.86 / 0.43 / 437.60
Beiyun River / BY01 / 28.7 / 7.61 / 3.17 / 870.1 / 15.7 / 49 / 0.76 / 11.62 / 0.84 / 0.42 / 435.10
BY02 / 28.4 / 8.51 / 13.23 / 762.8 / 25.9 / 53 / 0.85 / 3.50 / 1.95 / 0.37 / 381.40
BY03 / 30.3 / 7.73 / 19.7 / 1411.0 / 70.3 / 20 / 0.19 / 1.43 / 0.00 / 0.70 / 705.80
BY04 / 30.6 / 8.17 / 21.69 / 1362.0 / 14.4 / 38 / 0.91 / 4.93 / 1.46 / 0.68 / 681.10
BY05 / 32.3 / 7.76 / 8.85 / 920.2 / 56.9 / 15 / 1.96 / 9.94 / 1.20 / 0.45 / 459.40
BY06 / 29.2 / 7.78 / 5.9 / 1162.0 / 215 / 54 / 0.51 / 6.85 / 1.05 / 0.57 / 577.30
BY07 / 31.0 / 7.39 / 6.39 / 36970.0 / 206 / N.D / 0.06 / 2.21 / 0.17 / 23.37 / 18500.00

(d)  September

T / pH / O2 / Conductivity / Turbidity / COD / DIP / NH3-N / NO3-N / Salinity / TDS
°C / mg/L / µS/cm / NTU / mg/L / mgP/L / mgN/L / mgN/L / PSU / ppm
Yang River / YD01 / 15.7 / 8.16 / 9.29 / 2416.0 / 15.20 / 32 / 0.14 / 0.09 / 10.50 / 1.30 / 1250.00
YD02 / 19.1 / 8.71 / 7.59 / 255.6 / 8.19 / 18 / 0.03 / 1.10 / 0.67 / 0.12 / 127.50
YD03 / 19.5 / 8.18 / 9.21 / 656.1 / 9.13 / 6 / 0.06 / 0.57 / 3.24 / 0.32 / 251.70
YD04 / 18.5 / 8.36 / 11.02 / 469.1 / 3.90 / 98 / 0.57 / 0.63 / 7.74 / 0.23 / 235.60
YD05 / 20.4 / 8.07 / 9.29 / 707.1 / 4.04 / 37 / 0.35 / 0.71 / 6.19 / 0.35 / 354.00
YD06 / 20.7 / 8.16 / 5.68 / 884.4 / 4.08 / 48 / 0.02 / 0.41 / 0.77 / 0.44 / 442.10
Wenyu River / WY01 / 22.2 / 7.39 / 4.97 / 410.8 / 19.40 / 10 / 1.64 / 3.50 / 5.26 / 0.20 / 205.30
WY02 / 23.6 / 8.07 / 13.13 / 688.9 / 16.40 / 17 / 1.04 / 0.72 / 4.93 / 0.34 / 346.50
WY03 / 22.4 / 7.74 / 5.48 / 706.4 / 14.40 / 17 / 1.40 / 4.37 / 1.69 / 0.35 / 354.10
WY04 / 23.6 / 7.48 / 5.64 / 841.5 / 10.20 / 11 / 0.37 / 8.11 / 6.21 / 0.41 / 417.60
WY05 / 23.3 / 7.50 / 5.07 / 762.5 / 8.17 / 16 / 0.65 / 6.56 / 4.07 / 0.38 / 380.60
WY06 / 22 / 7.40 / 1.38 / 915.4 / 9.10 / 34 / 0.40 / 10.21 / 5.11 / 0.45 / 455.00
WY07 / 22.8 / 7.38 / 3.25 / 904.9 / 3.73 / 21 / 0.57 / 7.33 / 4.26 / 0.45 / 452.10
Beiyun River / BY01 / 22.4 / 7.48 / 2.83 / 867.8 / 5.88 / 17 / 1.39 / 8.19 / 3.94 / 0.37 / 378.60
BY02 / 23.2 / 7.81 / 13.85 / 764.5 / 7.33 / 14 / 0.50 / 0.59 / 5.19 / 0.28 / 286.50
BY03 / 23.7 / 7.92 / out / 1389.0 / 36.20 / 19 / 0.05 / 0.63 / 0.70 / 0.43 / 566.00
BY04 / 24.7 / 8.08 / 13.04 / 1381.0 / 6.69 / 21 / 0.69 / 2.35 / 2.53 / 0.69 / 687.00
BY05 / 23.3 / 7.62 / 1.42 / 1370.0 / 11.60 / 28 / 0.78 / 4.31 / 1.49 / 0.67 / 668.20
BY06 / 22.4 / 7.79 / 3.40 / 2366.0 / 16.60 / 35 / 0.76 / 2.92 / 3.26 / 1.23 / 1183.00
BY07 / 23 / 7.57 / 6.47 / 36530.0 / 18.30 / N.D / 0.08 / 2.21 / 1.43 / 23.11 / 18320.00

Figure S1 Total coliforms and fecal coliforms in four seasons