Qingqing Huang, Qi Wang, Zhang Luo, Yao Yu, Rongfeng Jiang, Huafen Li*

Effects of root iron plaque on selenite and selenate speciation in rhizosphere and uptake by rice (Oryza sativa)

Qingqing Huang, Qi Wang, Zhang Luo, Yao Yu, Rongfeng Jiang, Huafen Li*

Supporting data

Tables = 3

Figs. = 4

Table S1 Results of analysis of variance for the water management and Se treatment

Rice cultivar / Se treatment / Cultivar × Se
F ratio / Sig. (P) / F ratio / Sig. (P) / F ratio / Sig. (P)
Seedling stage / Root yield / 31.87 / 0.0001 / 0.54 / 0.5953 / 1.18 / 0.3410
Shoot yield / 0.43 / 0.5250 / 0.2 / 0.8227 / 0.79 / 0.4759
Mature stage / Root yield / 173.05 / < 0.0001 / 10.87 / 0.0020 / 26.03 / <0.0001
Straw yield / 188.77 / <0.0001 / 8.11 / 0.0059 / 40.58 / <0.0001
Husk yield / 62.58 / <0.0001 / 14.41 / 0.0006 / 31.86 / <0.0001
Grain yield / 0.22 / 0.6510 / 2.78 / 0.1017 / 11.88 / 0.0014
Seedling stage / Root Se concentration / 6.33 / 0.0215 / 12.84 / 0.0003 / 1.94 / 0.1723
Shoot Se concentration / 0.30 / 0.5903 / 69.61 / <0.0001 / 1.81 / 0.1924
Seedling stage / Ratios of shoot-Se to root-Se / 6.06 / 0.0241 / 13.83 / 0.0002 / 0.07 / 0.9323
Mature stage / Root Se concentration / 5.19 / 0.0419 / 6.49 / 0.0123 / 0.73 / 0.5032
Straw Se concentration / 0.19 / 0.6732 / 11 / 0.0019 / 0.46 / 0.6427
Husk Se concentration / 20.57 / 0.0007 / 6.28 / 0.0136 / 4.18 / 0.0419
Grain Se concentration / 4.56 / 0.054 / 5.09 / 0.0251 / 0.27 / 0.7645
Seedling stage / Se in DCB extracts / 108.81 / <0.0001 / 1.63 / 0.2226 / 0.05 / 0.9511
Mature stage / Se in DCB extracts / 9.85 / 0.0086 / 11.17 / 0.0018 / 1.57 / 0.2475
Seedling stage / Fe in DCB extracts / 108.81 / <0.0001 / 1.63 / 0.2226 / 0.05 / 0.9511
Mature stage / Fe in DCB extracts / 62.98 / <0.0001 / 0.76 / 0.4817 / 0.57 / 0.5738
Seedling stage / Root-Se% / 9.68 / 0.0090 / 1.79 / 0.2081 / 3.17 / 0.0784
Shoot-Se% / 1.92 / 0.1915 / 89.44 / <0.0001 / 3.23 / 0.0753
DCB-Se% / 19.18 / 0.0009 / 148.1 / <0.0001 / 9.04 / 0.004
Mature stage / Root-Se% / 28.12 / 0.0002 / 3.59 / 0.0600 / 0.52 / 0.6050
Shoot-Se% / 24.7 / 0.0003 / 3.56 / 0.0610 / 2.98 / 0.0889
DCB-Se% / 5.54 / 0.0365 / 10.77 / 0.0021 / 1.60 / 0.2424

Table S2 Biomass of rice at seedling stage and maturity (g pot-1) and the analysis of variance

Cultivar / Treatment / Seedling stage / Maturity
root / shoot / root / straw / grain / husk
Longjing21 / Control / 0.48±0.11 b / 1.81±0.28 a / 0.79±0.07 c / 3.05±0.29 e / 0.98±0.24 c / 0.26±0.07 c
Selenite / 0.57±0.05 b / 1.95±0.20 a / 1.04±0.09 c / 5.11±0.49 d / 1.87±0.51 ab / 0.33±0.09 c
Selenate / 0.49±0.04 b / 1.63±0.24 a / 1.03±0.20 c / 5.67±0.79 d / 1.88±0.38 ab / 0.44±0.08 c
Zhunliangyou608 / Control / 0.86±0.07 a / 1.62±0.07 a / 2.89±0.07 a / 13.00±0.31 a / 2.92±0.49 a / 1.23±0.07 a
Selenite / 0.85±0.06 a / 1.68±0.10 a / 1.65±0.10 b / 7.28±0.41 c / 1.72±0.15 b / 0.78±0.08 b
Selenate / 1.03±0.14 a / 1.79±0.09 a / 1.83±0.12 b / 9.12±0.48 b / 0.48±0.04 c / 0.33±0.09 c
Analysis of variance*
Rice cultivar (A) / P=0.0001 / P=0.5250 / P=< 0.0001 / P=<0.0001 / P=0.6510 / P=<0.0001
Se treatment (B) / P=0.5953 / P=0.8227 / P=0.0020 / P=0.0059 / P=0.1017 / P=0.0006
A×B / P=0.3410 / P=0.4759 / P=<0.0001 / P=<0.0001 / P=0.0014 / P=<0.0001

* The different letters within the same column indicate the significant differences according to LSD multiple comparison test (P < 0.05). Data are mean ± SE (n =4).

Table S3 Ratios of shoot-Se to root-Se at the seedling stage (Data are mean ± SE (n =4)).

Treatment / Longjing21 / Zhunliangyou608
Control / 0.30±0.03 b / 0.54±0.09 b
Selenite / 0.48±0.05 b / 0.73±0.05 ab
Selenate / 0.90±0.12 a / 1.07±0.21 a
Analysis of variance*
Rice cultivar (A) / P=0.0241
Se treatment (B) / P=0.0002
A×B / P=0.9323

Transfer factor=Shoot concentration/Root concentration. *The different letters within the same column indicate the significant differences according to LSD multiple comparison test (P < 0.05). Data are mean ± SE (n = 3).

Fig.S1 Changes in soil solution pH in the pot experiment: control (a), +selenite treatment (b), +selenate treatment (c). Data are mean ± SE (n =4).

Fig. S2 Dynamics of Fe concentrations in soil solution in the pot experiment: control (a), +selenite treatment (b), +selenate treatment (c). Data are mean ± SE (n =4).

Fig. S3 Dynamics of total Se concentrations in soil solution in the pot experiment: control (a), +selenite treatment (b), +selenate treatment (c). Data are mean ± SE (n = 4).

Fig. S4 Effects of rice cultivar and Se addition on the total Se concentration in rice straw (a) and husk (b) at grain maturity. The different letters indicate the significant differences according to LSD multiple comparison test (P < 0.05). Data are mean ± SE (n =4).