/503 / Public ID / Alternative Names / References of known
PU.1 target genes
1 / 2177 / NM_009972 / casein beta
2 / 1547 / NM_009892 / chitinase3 - like 3, neutrophil granules
3 / 1162 / NM_133246 / HTM4, hematopoietic kinase
4 / 1000 / AY065557 / chitinase 3-like 4, Ym2
5 / 727 / NM_008611 / neutrophil collagenase / (1)
6 / 594 / NM_011210 / Ly5, CD45 / (2)
7 / 545 / NM_011662 / Dap12, protein kinase tyrosine kinase / (3)
8 / 248 / BM224327 / CD32 / (4)
9 / 226 / D87867 / UDP glucuronosyltransferase 1 family
10 / 182 / NM_008935 / AC133, CD133
11 / 169 / NM_021364 / MDL-1, myeloid DAP12 asscociating lectin
12 / 166 / BC003855 / hypothetical gene
13 / 157 / BB324660 / hexokinase 3
14 / 150 / BB550124 / transglutaminase 2, TG2
15 / 143 / NM_010824 / myeloperoxidase / (5)
16 / 123 / AF143181 / immunoglobulin gamma Fc receptor I / (6)
17 / 100 / NM_030691 / immunoglobulin superfamily, member 6
18 / 81 / BB775785 / paired immunoglobin-like type 2 receptor
19 / 80 / L16462 / B-cell leukemia/lymphoma 2 related protein A1a
20 / 78 / BB800282 / complement factor properdin
21 / 77 / NM_031198 / transcription factor EC / (7)
22 / 76 / AW208566 / lysozyme / (8)
23 / 67 / AY042192 / MAS-related GPR, member A2
24 / 61 / NM_133209 / paired immunoglobin-like type 2 receptor beta
25 / 61 / U66888 / Ly71; F4/80 / (9)
26 / 52 / AK020608 / -
27 / 51 / BC026563 / leupaxin
Table 1. DNA microarray analysis of 503 PU.1-null cells compared to 503 PU.1 rescued cells. The PU.1null myeloid cell line 503 was originally isolated from a mouse neonatalPU.1 null liver and was maintained as described in (1). One microgram total RNA was used to synthesize cRNA. The cRNA was fragmented and hybridized to GNF1M Gene Chip arrays (10) according to standard Affymetrix protocols. Duplicate amplifications and hybridizations were performed from two total RNA preparations. Raw expression values were normalized within each Chip by dividingthe median expression value of the Chip. Results are given as n-fold induction of 503 PU.1 compared to 503 cells, and genes that were more than 50-fold induced are shown.
References
1.Anderson KL, Smith KA, Perkin H, Hermanson G, Anderson CG, Jolly DJ, et al. PU.1 and the granulocyte- and macrophage colony-stimulating factor receptors play distinct roles in late-stage myeloid cell differentiation. Blood. 1999 Oct 1;94(7):2310-8.
2.Anderson KL, Nelson SL, Perkin HB, Smith KA, Klemsz MJ, Torbett BE. PU.1 is a lineage-specific regulator of tyrosine phosphatase CD45. J Biol Chem. 2001 Mar 9;276(10):7637-42.
3.Henkel GW, McKercher SR, Maki RA. Identification of three genes up-regulated in PU.1 rescued monocytic precursor cells. Int Immunol. 2002 Jul;14(7):723-32.
4.Bonfield TL, Raychaudhuri B, Malur A, Abraham S, Trapnell BC, Kavuru MS, et al. PU.1 regulation of human alveolar macrophage differentiation requires granulocyte-macrophage colony-stimulating factor. Am J Physiol Lung Cell Mol Physiol. 2003 Nov;285(5):L1132-6.
5.Wang X, Scott E, Sawyers CL, Friedman AD. C/EBPalpha bypasses granulocyte colony-stimulating factor signals to rapidly induce PU.1 gene expression, stimulate granulocytic differentiation, and limit proliferation in 32D cl3 myeloblasts. Blood. 1999 Jul 15;94(2):560-71.
6.Aittomaki S, Yang J, Scott EW, Simon MC, Silvennoinen O. Molecular basis of Stat1 and PU.1 cooperation in cytokine-induced Fcgamma receptor I promoter activation. Int Immunol. 2004 Feb;16(2):265-74.
7.Rehli M, Lichanska A, Cassady AI, Ostrowski MC, Hume DA. TFEC is a macrophage-restricted member of the microphthalmia-TFE subfamily of basic helix-loop-helix leucine zipper transcription factors. J Immunol. 1999 Feb 1;162(3):1559-65.
8.Faust N, Bonifer C, Sippel AE. Differential activity of the -2.7 kb chicken lysozyme enhancer in macrophages of different ontogenic origins is regulated by C/EBP and PU.1 transcription factors. DNA Cell Biol. 1999 Aug;18(8):631-42.
9.O'Reilly D, Addley M, Quinn C, MacFarlane AJ, Gordon S, McKnight AJ, et al. Functional analysis of the murine Emr1 promoter identifies a novel purine-rich regulatory motif required for high-level gene expression in macrophages. Genomics. 2004 Dec;84(6):1030-40.
10.Su AI, Cooke MP, Ching KA, Hakak Y, Walker JR, Wiltshire T, et al. Large-scale analysis of the human and mouse transcriptomes. Proc Natl Acad Sci U S A. 2002 Apr 2;99(7):4465-70.