Supplementary Table 1. Bacterial Strains and Plasmids Used in This Study

Supplementary Table 1. Bacterial strains and plasmids used in this study.

Strains or plasmids / Relevant characteristic(s)a / Reference
E. coli
DH5α / supE44ΔlacU169(Φ80 lacZΔM15) hsdR17 recA1 endA1 gyrA96 thi-1 relA1. / TaKaRa
PEDRP0 / Apr, Cmr, Kmr, E. coli DH5α contains two plasmids pET28a and pPR9P-14. / This study
PEDRP14 / Apr, Cmr, Kmr, E. coli DH5α contains two plasmids pEDR and pPR9P-14. / This study
A. calcoaceticus
NCIB8250 / Phenol degrader, wild type. / Schirmer et al. (1997)
PHEA-2 / Phenol degrader, wild type, Apr. / Xu et al. (2003)
Plasmids
pWH841 / Apr, Pmop::lacZ, contains the mopR gene of A. calcoaceticus NCIB8250. / Schirmer et al. (1997)
pET28a / Kmr, 5.1 kb expression vector. / Stratagene
pEDR / Kmr, 6.8 kb pET28a contains 1683 bp of the mopR gene placed under control of the T7 promoter. / This study
pPR9TT / Apr Cmr, 9.3 kb lacZ-based promoter-probe vector. / Santos et al. (2000)
pPR9P-14 / Apr Cmr, 9.75 kb pPR9TT derivative with P14 derivative of PmphK. / This study
pPR9P-24 / Apr Cmr, 9.6 kb pPR9TT derivative with P24 derivative of PmphK. / This study
pPR9P-34 / Apr Cmr, 9.6 kb pPR9TT derivative with P34 derivative of PmphK. / This study
pPR9P-44 / Apr Cmr, 9.6 kb pPR9TT derivative with P44 derivative of PmphK. / This study
pPR9P-54 / Apr Cmr, 9.6 kb pPR9TT derivative with P54 derivative of PmphK. / This study
pPR9P-64 / Apr Cmr, 9.6 kb pPR9TT derivative with P64 derivative of PmphK. / This study
pPR9P-74 / Apr Cmr, 9.6 kb pPR9TT derivative with P74 derivative of PmphK. / This study

Apr, ampicillin resistant; Cmr, chloramphenicol resistant; Kmr, kanamycin resistant.

Supplementary Table 2. Primers used in this study.

Primers / Sequencesa
P-up1 / 5’-CGCCTCGAGGAGTGATCTGTTTTAATCC-3’
P-up2 / 5’-CGCCTCGAGTAAAGCCTCAAACAATCT-3’
P-up3 / 5’-CGCCTCGAGTTTACGACCCTATTCATCAA-3’
P-up4 / 5’-CGCCTCGAGGACCCTATTCATCAAATCAT-3’
P-up5 / 5’-CGCCTCGAGTCATCAAATCATGAATATCTC-3’
P-up6 / 5’-CGCCTCGAGTCAAATCATGAATATCTCTA-3’
P-up7 / 5’-CGCCTCGAGTCTCTATTTCACCATTCA-3’
P-down4 / 5’-CCTTCTGCAGTCGAATTCGATAAACTCTG-3’
MOPR-F / 5’-AACTGCAGGATATGAACTACGACAG-3’
MOPR-R / 5’-CGGAATTCCAAGCACATTTCTATG-3’

a The bolded sequences in italics represent the EcoRI (GAATTC), PstI (CTGCAG), and XhoI (CTCGAG) restriction sites.