Supplemental Data I
PROTEIN CONSERVED DOMAIN STRUCTURES, AND ALIGNMENTS WITH KNOWN PROTEINS
TaRSZ38
AtRSZ33 ACCESSION CAC03605
AUTHORS Lopato,S., Forstner,C., Kalyna,M., Hilscher,J., Langhammer,U.,
Indrapichate,K., Lorkovic,Z.J. and Barta,A.
TITLE Network of interactions of a novel plant-specific Arg/Ser-rich
protein, atRSZ33, with atSC35-like splicing factors
JOURNAL J. Biol. Chem. 277 (42), 39989-39998 (2002)
PUBMED 12176998
1 50
AtRSZ33 (1) MPRYDDRYGNTRLYVGRLSSRTRTRDLERLFSRYGRVRDVDMKRDYAFVE
TaRSZ38 (1) MPRYDDRYGNARLYVGRLSSRTRSRDLEYLFSKYGRIREVELKRDYAFIE
51 100
AtRSZ33 (51) FGDPRDADDARHYLDGRDFDGSRITVEFSRGAPRGS-----RDFDSRGPP
TaRSZ38 (51) YSDPRDADEARYNLDGRDVDGSRIIVEFAKGVPRGSGGSREREYVGRGPP
101 150
AtRSZ33 (96) PGAGRCFNCGVDGHWARDCTAGDWKNKCYRCGERGHIERNCKNQPKKLRR
TaRSZ38 (101) PGTGRCFNCGIDGHWARDCKAGDWKNKCYRCGERGHIERNCQNSPRSLRR
151 200
AtRSZ33 (146) SGSYSRSPVRSRSPRRRRSPSRSLSRSGSYSRSRS------PVRRRERSV
TaRSZ38 (151) ERSYSRSPS----PRRGRARSRSYSRSRSYSRSRSRSYSESPRGRRTERD
201 250
AtRSZ33 (190) EERSR------SPKR------MDDSLSPR-----ARDRSPVLDDEGS-
TaRSZ38 (197) ERRSRSISYSRSPRRSLSPGGKEMDRSPTPDRSRSPRRSISPVAKDNGDS
251 300
AtRSZ33 (220) PKIIDGSPPPSPKLQKEVGSDRDGGSPQDNG-----RNSVVSPVVGAGGD
TaRSZ38 (247) PRGRETSRSPSDGYRSPVANGRSPRSPVNNGSPSPTRDNRASPSLRGNNG
301 338
AtRSZ33 (265) S------SKEDRSPVDDDYEPNRTSPRG-SESP-
TaRSZ38 (297) SPSPKGNGNGGSPSPRGNGDDDGRRGSGSPRGRSVSP-
Consensus positions: 61,5%; Identity positions: 54.4%
TaDRH1 (partial)
AtDRH1 ACCESSION BAA28347
AUTHORS Okanami,M., Meshi,T. and Iwabuchi,M.
TITLE Characterization of a DEAD box ATPase/RNA helicase protein of
Arabidopsis thaliana
JOURNAL Nucleic Acids Res. 26 (11), 2638-2643 (1998)
NtDB10 ACCESSION P46942
AUTHORS Itadani,H., Sugita,M. and Sugiura,M.
TITLE Structure and expression of a cDNA encoding an RNA helicase-like
protein in tobacco
JOURNAL Plant Mol. Biol. 24 (1), 249-252 (1994)
Hsp72 ACCESSION AAC50787
AUTHORS Lamm,G.M., Nicol,S.M., Fuller-Pace,F.V. and Lamond,A.I.
TITLE p72: a human nuclear DEAD box protein highly related to p68
JOURNAL Nucleic Acids Res. 24 (19), 3739-3747 (1996)
1 50
AtDRH1 (1) -MAATAAASVVRYAPEDHTLPKPWKGLIDDRTGYLYFWNPETNVTQYEKP
Hsp72 (1) ------MRGGGFGDRDRDRDRGGFGARGGG-G------
NtDB10 (1) MAVVTASSAGPSYAPEDPTLPKPWKGLVDGTTGFIYFWNPETNDTQYERP
TaDRH1_partial (1) ------
51 100
AtDRH1 (50) TPSLPPKFSPAVSVSSSVQVQQTDAYAPPKDDDKYSRGSERVSRFSEGGR
Hsp72 (26) ------LPPK--KFG------
NtDB10 (51) VPSSHAVSAPAH-KSS---VFVSSSVE--KPSQGQ------RYDADGG
TaDRH1_partial (1) ------
101 150
AtDRH1 (100) SGPPYSNGAANGVGDSAYGAASTRVPLPSSAPASELSPEAYSRRHEITVS
Hsp72 (33) NPGERLRKKKWDLSELPKFEKNFYVEHPEVARLTPYEVDELRRKKEITVR
NtDB10 (87) HNRGSNNKIARSSSDRFHDGTSVHEGYGSLGVGSDISQESYCRRNEISVT
TaDRH1_partial (1) ------
151 200
AtDRH1 (150) GGQVP-PPLMSFEATGFPPELLREVLSAGFSAPTPIQAQSWPIAMQGRDI
Hsp72 (83) GGDVCPKPVFAFHHANFPQYVMDVLMDQHFTEPTPIQCQGFPLALSGRDM
NtDB10 (137) GGDVP-APLTSFEATGFPSEIVREMHQAGFSAPTPIQAQSWPIALQGRDI
TaDRH1_partial (1) ------
201 250
AtDRH1 (199) VAIAKTGSGKTLGYLIPGFLHLQRIRNDSR-MGPTILVLSPTRELATQIQ
Hsp72 (133) VGIAQTGSGKTLAYLLPAIVHINHQPYLERGDGPICLVLAPTRELAQQVQ
NtDB10 (186) VAIAKTGSGKTLGYLMPAFIHLQQRRKNPQ-LGPTILVLSPTRELATQIQ
TaDRH1_partial (1) ------
251 300
AtDRH1 (248) EEAVKFGRSSRISCTCLYGGAPKGPQLRDLERGADIVVATPGRLNDILEM
Hsp72 (183) QVADDYGKCSRLKSTCIYGGAPKGPQIRDLERGVEICIATPGRLIDFLES
NtDB10 (235) AEAVKFGKSSRISCTCLYGGAPKGPQLRELSRGVDIVVATPGRLNDILEM
TaDRH1_partial (1) ------GAPKGPQLRDLERGADIVVATPGRLNDILEM
301 350
AtDRH1 (298) RRISLRQISYLVLDEADRMLDMGFEPQIRKIVKEIPTKRQTLMYTATWPK
Hsp72 (233) GKTNLRRCTYLVLDEADRMLDMGFEPQIRKIVDQIRPDRQTLMWSATWPK
NtDB10 (285) RRVSLGQVSYLVLDEADRMLDMGFEPQIRKIVKEVPVQRQTLMYTATWPK
TaDRH1_partial (32) GKVSLRQVAYLVLDEADRMLDMGFEPQIRKIVKQVQPKRQTLMFTATWPR
351 400
AtDRH1 (348) GVRKIAADLLVNPAQVNIGNVDELVANKSITQHIEVVAPMEKQRRLEQIL
Hsp72 (283) EVRQLAEDFLRDYTQINVGN-LELSANHNILQIVDVCMESEKDHKLIQLM
NtDB10 (335) GVRKIAADLLVNSVQVNIGNVDELVANKSITQHIEVVLPMEKQRRVEQIL
TaDRH1_partial (82) EVRKIASDLLTNPVQVNIGNTDELVANKSITQHVEVTTSFEKGRRLDQIL
401 450
AtDRH1 (398) RSQEPG--SKVIIFCSTKRMCDQLTRNLTRQ-FGAAAIHGDKSQPERDNV
Hsp72 (332) EEIMAEKENKTIIFVETKRRCDDLTRRMRRDGWPAMCIHGDKSQPERDWV
NtDB10 (385) RSKEPG--SKIIIFCSTKKMCDQLSRNLTRN-FGAAAIHGDKSQGERDYV
TaDRH1_partial (132) RQQEPG--SKVIIFCSTKRMCDQLSRNLSRQ-YGASAIHGDKSQAERDSV
451 500
AtDRH1 (445) LNQFRSGRTPVLVATDVAARGLDVKDIRAVVNYDFPNGVEDYVHRIGRTG
Hsp72 (382) LNEFRSGKAPILIATDVASRGLDVEDVKFVINYDYPNSSEDYVHRIGRTA
NtDB10 (432) LSQFRAGRSPVLVATDVAARGLDIKDIRVVINYDFPTGIEDYVHRIGRTG
TaDRH1_partial (179) LSEFRTGRCPILVATDVAARGLDVKDIRVVVNYDFPTGVEDYVHRIGRTG
501 550
AtDRH1 (495) RAGATGQAFTFFGDQDSKHASDLIKILEGANQRVPPQIREMATR--GGGG
Hsp72 (432) RSTNKGTAYTFFTPGNLKQARELIKVLEEANQAINPKLMQLVDHRGGGGG
NtDB10 (482) RAGASGLAYTFFSDQDSKHALDLVKVLEGANQCVPTELRDMASR--GGGM
TaDRH1_partial (229) RAGATGIAYTFFCDQDSKYASDLVKILEGANQNVSPELRAMVGR--GGYG
551 600
AtDRH1 (543) MNKFSRWGPPSGGRG------R
Hsp72 (482) GGGRSRYRTTSSANNPNLMYQDECDRRLRGVKDGGRRDSASYRDRSETDR
NtDB10 (530) GRARNHWGSGPGGRGG------R
TaDRH1_partial (277) GRGPRRWASSNDSYGG------Q
601 650
AtDRH1 (559) GG------DSGYGGR--GSFASRDSRSSNGWGRERERSRSPERFNR
Hsp72 (532) AGYANGSGYGSPNSAFGAQAGQYTYGQGTYGAAAYGTSSYTAQEYGAGTY
NtDB10 (547) GGPY------NSSYVGRNGGHGYDRGSRDSDRYGHGTYNADAPRKRSR
TaDRH1_partial (294) GAYG------SQTRDGPSFQSSFNNSSSGNQYGGAPSFHTSSSNNQTS
651 700
AtDRH1 (597) APPP-SSTGSPPRSFHETMMMKHR------
Hsp72 (582) GASSTTSTGRSSQSSSQQFSGIGRSGQQPQPLMSQQFAQPPGATNMIGYM
NtDB10 (589) SRSPNIGSGWSGKKSRFTD------
TaDRH1_partial (336) GAASLPASGGSGEGLSFHDRFYSGNSRGGDRARSRSPPKAVGVSNW----
701 719
AtDRH1 (620) ------
Hsp72 (632) GQTAYQYPPPPPPPPPSRK
NtDB10 (608) ------
TaDRH1_partial (382) ------
Consensus positions: 40.6%; Identity positions: 36.5% (for TaDRH1-partial and AtDRH1)
TaLuc7
HsLuc7b ACCESSION NP_958815
AUTHORS Tufarelli,C., Frischauf,A.M., Hardison,R., Flint,J. and Higgs,D.R.
TITLE Characterization of a widely expressed gene (LUC7-LIKE; LUC7L)
defining the centromeric boundary of the human alpha-globin domain
JOURNAL Genomics 71 (3), 307-314 (2001)
ScLuc7p ACCESSION NP_010196
AUTHORS Jacq,C., Alt-Morbe,J., Andre,B., Arnold,W., Bahr,A., Ballesta,J.P.,
Bargues,M., Baron,L., Becker,A., Biteau,N., Blocker,H.,
Blugeon,C., Boskovic,J., Brandt,P., Bruckner,M., Buitrago,M.J.,
Coster,F., Delaveau,T., del Rey,F., Dujon,B., Eide,L.G., Garcia-
Cantalejo,J.M., Goffeau,A., Gomez-Peris,A., Zaccaria,P. et al.
TITLE The nucleotide sequence of Saccharomyces cerevisiae chromosome IV
JOURNAL Nature 387 (6632 Suppl), 75-78 (1997)
PUBMED 9169867
REFERENCE 2 (residues 1 to 261)
AUTHORS Goffeau,A., Barrell,B.G., Bussey,H., Davis,R.W., Dujon,B.,
Feldmann,H., Galibert,F., Hoheisel,J.D., Jacq,C., Johnston,M.,
Louis,E.J., Mewes,H.W., Murakami,Y., Philippsen,P., Tettelin,H. and
Oliver,S.G.
TITLE Life with 6000 genes
JOURNAL Science 274 (5287), 546-547 (1996)
1 50
HsLuc7b (1) ----MSAQAQMRALLDQLMGTARDG-----DETRQRVKFTDDRVCKSHLL
ScLuc7p (1) MSTMSTPAAEQRKLVEQLMGRDFSFRHNRYSHQKRDLGLHDPKICKSYLV
TaLuc7 (1) ------MDAMRKQLDVLMGANRNG-----DVREVNRKYFDRDVCRLFLA
51 100
HsLuc7b (42) DCCPHDILAGTRMDLGECTKIHDLALRADYEIASKER--DLFFELDAMDH
ScLuc7p (51) GECPYDLFQGTKQSLGKCPQMHLTKHKIQYEREVKQGKTFPEFEREYLAI
TaLuc7 (39) GLCPHDLFQLTKMDLGPCPKVHSLQLRKDYEEVKAKG--TENFDRELEDM
101 150
HsLuc7b (90) LESFIAECDRRTELAKKRLAETQEEISAEVSA----KAEKVHELNEEIGK
ScLuc7p (101) LSRFVNECNGQISVALQNLKHTAEERMKIQQVT-----EELDVLDVRIGL
TaLuc7 (87) IDRLIVECERKIQRALKRLADEDAKAAIAISVSEVTQSEEVAQLSKEIKE
151 200
HsLuc7b (136) LLAKAEQLGAEGNVDESQKILMEVEKVRAK------
ScLuc7p (146) MGQEIDSLIRADEVSMGMLQSVKLQELISKR------
TaLuc7 (137) KMKEADIFDFEGKTDDKIKTMELVEELRSKRADLQATLLLDAFNKDRAAI
201 250
HsLuc7b (166) ------KKEAE
ScLuc7p (177) ------KEVAK
TaLuc7 (187) PQPIPPPQMATLPAPPPPDARTQELINEKLSKAEALGEQGMVEEAQKALE
251 300
HsLuc7b (171) EEYRNSMPASSFQQ------QKLRVCEVCSAYLGLHDND
ScLuc7p (182) RVRNITENVGQSAQ------QKLQVCEVCGAYLSRLDTD
TaLuc7 (237) EAEALKKLAAARQEPVPDPSKYSVADVRITDQKLRLCDICGAFLSVYDND
301 350
HsLuc7b (204) RRLADHFGGKLHLGFIQIREKLDQLRKTVAEKQEKRNQDRLRRREERERE
ScLuc7p (215) RRLADHFLGKIHLGYVKMREDYDRLMK------N------
TaLuc7 (287) RRLADHFGGKLHLGYMLIREKLKELQ------EERN----KKRTEKPED
351 400
HsLuc7b (254) ERLSRRSGSRTRDRRRSRSRDRRRRRSRSTSRERRKLSRSRSRDRHRRHR
ScLuc7p (243) -----NR---TTNASKTATTLPGRRFV------
TaLuc7 (326) DRRSREN-SRDRNGRASRDRDAERKDRVEPRDSRRDHDRDRDR-RHDRDR
401 450
HsLuc7b (304) SRSRSHSRGHRRASRDRSAKYKFSRERASREESWESGRSERGPPDWRLES
ScLuc7p (262) ------
TaLuc7 (374) RHDRDRDRDHDRSSRGREHDRDRRRERS------RS------
451 468
HsLuc7b (354) SNGKMASRRSEEKEAGEI
ScLuc7p (262) ------
TaLuc7 (404) ---RDRSRRHERY-----
Consensus positions: 38.7%; Identity positions: 30.0% (for TaLuc7 and ScLuc7p)
TaPrp38
ScPrp38p ACCESSION NP_011589
DEFINITION Unique component of the U4/U6.U5 tri-snRNP particle, dispensable
for spliceosome assembly, but required for conformational changes
which lead to catalytic activation of the spliceosome; Prp38p
[Saccharomyces cerevisiae].
AUTHORS Tettelin,H., Agostoni Carbone,M.L., Albermann,K., Albers,M.,
Arroyo,J., Backes,U., Barreiros,T., Bertani,I., Bjourson,A.J.,
Bruckner,M., Bruschi,C.V., Carignani,G., Castagnoli,L.,
Cerdan,E., Clemente,M.L., Coblenz,A., Coglievina,M., Coissac,E.,
Defoor,E., Del Bino,S., Delius,H., Delneri,D., de Wergifosse,P.,
Dujon,B., Kleine,K. et al.
TITLE The nucleotide sequence of Saccharomyces cerevisiae chromosome VII
JOURNAL Nature 387 (6632 Suppl), 81-84 (1997)
PUBMED 9169869
AUTHORS Goffeau,A., Barrell,B.G., Bussey,H., Davis,R.W., Dujon,B.,
Feldmann,H., Galibert,F., Hoheisel,J.D., Jacq,C., Johnston,M.,
Louis,E.J., Mewes,H.W., Murakami,Y., Philippsen,P., Tettelin,H. and
Oliver,S.G.
TITLE Life with 6000 genes
JOURNAL Science 274 (5287), 546-547 (1996)
PUBMED 8849441
HsPrp38 ACCESSION NP_116253
AUTHORS Beausoleil,S.A., Jedrychowski,M., Schwartz,D., Elias,J.E.,
Villen,J., Li,J., Cohn,M.A., Cantley,L.C. and Gygi,S.P.
TITLE Large-scale characterization of HeLa cell nuclear phosphoproteins
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 101 (33), 12130-12135 (2004)
PUBMED 15302935
1 50
HsPrp38 (1) -----MANRTVKDAHSIHGTNPQYLVEKIIRTRIYESKYWKEECFG--LT
ScPrp38p (1) MAVNEFQVESNISPKQLNNQSVSLVIPRLTRDKIHNSMYYKVNLSNESLR
Ta Prp38 (1) -----MANRTDPRARSIHGTNPQNLVEKIVRAKIYQSNYWKEQCFG--LT
51 100
HsPrp38 (44) AELVVDKAMELRFVGGVYGGN------IKPTPFLCLTLKMLQIQP---
ScPrp38p (51) GNTMVELLKVMIGAFGTIKGQNGHLHMMVLGGIEFKCILMKLIEIRPNFQ
Ta Prp38 (44) AETLVDKAMELDYTGGTHGGN------RRPTPFLCLALKMLQIQP---
101 150
HsPrp38 (83) --EKDIIVEFIKNEDFKYVRMLGALYMRLTGTAIDCYKYLEPLYNDYRKI
ScPrp38p (101) QLNFLLNVKNENGFDSKYIIALLLVYARLQYYYLNGNNKNDDDENDLIKL
Ta Prp38 (83) --DKEIVVEFIKDEDYKYVRVLGAFYLRLTGTVADVYQYLEPLYNDYRKI
151 200
HsPrp38 (131) K------SQNRNGEFELMHVDEFIDELLHSER
ScPrp38p (151) FKVQLYKYSQHYFKLKSFPLQVDCFAHSYNEELCIIHIDELVDWLATQDH
Ta Prp38 (131) R------QKLSDGKFTLTHVDEFIDELLTKDY
201 250
HsPrp38 (157) VCDIILPRLQKRYVLEEAEQLEPRVSALEEDMDDVESSEEE------
ScPrp38p (201) IWGIPLGKCQWNKIYNSDEESSSSESESNGDSEDDNDTSSES------
Ta Prp38 (157) SCGTALPRIQKRWILEASGTLEPRRSALEDDFEEEEEDKEDGQPMDVDEP
251 300
HsPrp38 (198) --EEEDEKLERVPSPDHRR------RSYRDLDKPR-RSPTLRYRR
ScPrp38p (243) ------
Ta Prp38 (207) NTHEKDHLRGRSPTKERDRERERDRDRKHERHHRDRDHDRDRDHDRDYGR
301 350
HsPrp38 (234) SRSRSPRRRSRSPKRRSPSPRRERHRSKSPRRHRSRSRDRR------
ScPrp38p (243) ------
Ta Prp38 (257) GRERDRDRDRGRERDRERDRERDRHRIRDDDYHRDRDRDGRERERRDRDR
351 400
HsPrp38 (275) --HRSRSKSP------GHHRSHRHRSHSKSP------ERSK
ScPrp38p (243) ------
Ta Prp38 (307) GRHRSRSGSRSRDRRERDREVGELRKRRGRGSASPPRGRAEDGPREEPKK
401 438
HsPrp38 (302) KSHKKSRRGNE------
ScPrp38p (243) ------
Ta Prp38 (357) RKEKKEKKGSGNGPDPNDPEIIEMNKLRASIGLGPLK-
Consensus positions: 52.2%; Identity positions: 44.0% (for TaPrp38 and HsPrp38)
TaRSZ22
AtRSZ22 ACCESSION T05112
AUTHORS Golovkin,M. and Reddy,A.S.
TITLE The plant U1 small nuclear ribonucleoprotein particle 70K protein
interacts with two novel serine/arginine-rich proteins
JOURNAL Plant Cell 10 (10), 1637-1648 (1998)
PUBMED 9761791
AtRSZp22 ACCESSION CAA05352
AUTHORS Lopato,S., Gattoni,R., Fabini,G., Stevenin,J. and Barta,A.
TITLE A novel family of plant splicing factors with a Zn knuckle motif:
examination of RNA binding and splicing activities
JOURNAL Plant Mol. Biol. 39 (4), 761-773 (1999)
PUBMED 10350090
AtRSZp21 ACCESSION CAA05351
AUTHORS Lopato,S., Gattoni,R., Fabini,G., Stevenin,J. and Barta,A.
TITLE A novel family of plant splicing factors with a Zn knuckle motif:
examination of RNA binding and splicing activities
JOURNAL Plant Mol. Biol. 39 (4), 761-773 (1999)
1 50
AtRSZ22 (1) MSRVYVGNLDPRVTERELEDEFRAFGVVRSVWVARRPPGYAFLDFEDPRD
AtRSZp21 (1) MTRVYVGNLDPRVTERELEDEFKAFGVLRNVWVARRPPGYAFLEFDDERD
AtRSZp22 (1) MSRVYVGNLDPRVTERELEDEFRAFGVVRSVWVARRPPGYAFLDFEDPRD
TaRSC22a (1) MARLYVGNLDARVTAGELEDEFRVFGILRSVWVARKPPGFAFIDFDDKRD
TaRSZ22 (1) MARVYVGSLDPAVTARELEDEFRVFGVLRSVWVARKPPGFAFVDFDDRRD
51 100
AtRSZ22 (51) ARDAIRALDGKNGWRVEQSHNRGERGGGGRGGDRGGGGGGRGGRGGSDLK
AtRSZp21 (51) ALDAISALDRKNGWRVELSHKDKG-GRGGGGGRRGG------IEDSK
AtRSZp22 (51) ARDAIRALDGKNGWRVAQSHNRGERGGGGRGGDRGGGGAGRGGRGGSDLK
TaRSC22a (51) AEDALRDLDGKNGWRVELSRNDRG-DRGGRGGGGGGGGGGRG-RGGSDMK
TaRSZ22 (51) AQDAIKDLDGKNGWRVELSRNASS-GRGGRDRSGG------SEMK
101 150
AtRSZ22 (101) CYECGETGHFARECRNRGG-----TGRRRSKSRSRTPP---RYRRSPSYG
AtRSZp21 (91) CYECGELGHFARECRRGRG-----SVRRRSPSPRRRRS------PDYGYA
AtRSZp22 (101) CYECGETGHFARECRNRGG-----TGRRRSKSRSRTPP---RYRRSPSYG
TaRSC22a (99) CYECGESGHFARECRLRIGAGGLGSGRRRSRSRSRSRS--PRYRRSPSYG
TaRSZ22 (89) CYECGESGHFARECRLRIGSGGLGSGRRRSRSRSRSRSRSPRYRRSPSYS
151 200
AtRSZ22 (143) RRSYSPRARSPPPPRRRSPSPPPARGRSYSRSPPPYRAREEVPYANGNGL
AtRSZp21 (130) RRSISPSGRRS-PPRRR--SVTPPR--RYSRSPPYRGSRRDSPRRRDSPY
AtRSZp22 (143) RRSYSPRARSPPPPRRRSPSPPPARGRSYSRSPPPYRAREEVPYANGNGL
TaRSC22a (147) RRSYSPRDR---SPRRRSASPAPARGRSYSKSP--VRARDDSPDAKG---
TaRSZ22 (139) RRSYS--R----SPRRR--SVSPARGRSVSRSP--VRGT------
201
AtRSZ22 (193) KERRRSRS--
AtRSZp21 (175) GRRSPYANGV
AtRSZp22 (193) KERRRSRS--
TaRSC22a (189) --YRRSRS--
TaRSZ22 (168) ------
Consensus positions: 76.7%; Identity positions: 68.0% (for TaRSZ22a and AtRSZ22)
TaRSZ38BP1
No conserved domains
No similar proteins from dicotyledonous plants
No sequence orthologues in rice
However, several wheat ESTs were identified:
gi|9420538|gb|BE422695.1| WHE0058_H05_P10ZS Wheat endosperm cDNA library Triticum aestivum
cDNA clone WHE0058_H05_P10, mRNA sequence.
Length=592
Score = 179 bits (453), Expect = 2e-44
Identities = 86/93 (92%), Positives = 87/93 (93%), Gaps = 2/93 (2%)
Frame = -1
Query 387 MVGCCCCDPRVASPSQPMKYPAGNQQLTSISREANRICQTLVCQSGWTVTFNPRWCQRTL 446
MVGCCCCD RVA+PSQPMK PAGNQQLTSISREANRICQTLVCQSGWTVTFNPRWCQRTL
Sbjct 556 MVGCCCCDTRVANPSQPMKNPAGNQQLTSISREANRICQTLVCQSGWTVTFNPRWCQRTL 377
Query 447 TSVFDVGLGS--LPINRNDLLSLRFGCWSVLVL 477
TSVFDVGLGS L INRNDLLSLRF CWSVLVL
Sbjct 376 TSVFDVGLGSCYLSINRNDLLSLRFFCWSVLVL 278
Score = 28.9 bits (63), Expect = 2e-44
Identities = 11/11 (100%), Positives = 11/11 (100%), Gaps = 0/11 (0%)
Frame = -2
Query 375 VRVDGYLPDYP 385
VRVDGYLPDYP
Sbjct 591 VRVDGYLPDYP 559
gi|49520332|emb|AL814631.1| AL814631 h:116 Triticum aestivum cDNA clone G04_h116_plate_13,
mRNA sequence.
Length=612
Score = 147 bits (372), Expect = 1e-34
Identities = 81/129 (62%), Positives = 89/129 (68%), Gaps = 19/129 (14%)
Frame = +3
Query 213 SGNFGTSLSPPSHPTTEYGASRRH------GTTEY------VLPEHAANVSR----G 253
SG+ G ++P +Y RR EY VLPE A+V+ G
Sbjct 225 SGSRGPYMNPRGSGPADYEMERRSVPLHHDVPNVEEYTGRPLNMVLPEGIASVNTYSILG 404
Query 254 PYMNPRGSGPADYEMERRSVPLHHDVPNVEEYTGQPLNMVLAEGIAPVNTYSLRGESPGA 313
PY+NPRGSGPADYEMERRSVPLHHDVPNVEEYTGQ LNMVLAEGIAPV+TYSLRGESPGA
Sbjct 405 PYINPRGSGPADYEMERRSVPLHHDVPNVEEYTGQALNMVLAEGIAPVDTYSLRGESPGA 584
Query 314 YGPGTDAGM 322
Y PGTDAGM
Sbjct 585 YRPGTDAGM 611
Score = 145 bits (366), Expect = 5e-43
Identities = 74/100 (74%), Positives = 80/100 (80%), Gaps = 3/100 (3%)
Frame = +3
Query 220 LSPPSH-PTTEYGASRRHGTTEYVLPEHAANVSRGPYMNPRGSGPADYEMERRSVPLHHD 278
+S P H TEYGASRRHG+ Y EHAA+ SRGPYMNPRGSGPADYEMERRSVPLHHD
Sbjct 135 VSVPLHIRPTEYGASRRHGSPPYQRSEHAASGSRGPYMNPRGSGPADYEMERRSVPLHHD 314
Query 279 VPNVEEYTGQPLNMVLAEGIAPVNTYSLRGE--SPGAYGP 316
VPNVEEYTG+PLNMVL EGIA VNTYS+ G +P GP
Sbjct 315 VPNVEEYTGRPLNMVLPEGIASVNTYSILGPYINPRGSGP 434
Score = 57.4 bits (137), Expect = 5e-43
Identities = 26/26 (100%), Positives = 26/26 (100%), Gaps = 0/26 (0%)
Frame = +2
Query 202 RRLSPQQSALPSGNFGTSLSPPSHPT 227
RRLSPQQSALPSGNFGTSLSPPSHPT
Sbjct 83 RRLSPQQSALPSGNFGTSLSPPSHPT 160
TaSRp30
ZmSRP31 ACCESSION AAU29336
AUTHORS Gao,H., Gordon-Kamm,W.J. and Lyznik,L.A.
TITLE ASF/SF2-like maize pre-mRNA splicing factors affect splice site
utilization and their transcripts are alternatively spliced
JOURNAL Gene 339, 25-37 (2004)
PUBMED 15363843
AtSRp30 ACCESSION CAB42557
AUTHORS Lopato,S., Kalyna,M., Dorner,S., Kobayashi,R., Krainer,A.R. and
Barta,A.
TITLE atSRp30, one of two SF2/ASF-like proteins from Arabidopsis
thaliana, regulates splicing of specific plant genes
JOURNAL Genes Dev. 13 (8), 987-1001 (1999)
PUBMED 10215626
AtSR1 ACCESSION AAD52609
AUTHORS Lazar,G. and Goodman,H.M.
TITLE The Arabidopsis splicing factor SR1 is regulated by alternative
splicing
JOURNAL Plant Mol. Biol. 42 (4), 571-581 (2000)
PUBMED 10809003
1 50
AtSR1 (1) MSSRSSRTVYVGNLPGDIREREVEDLFSKYGPVVQIDLKVPPRPPGYAFV
AtSRp30 (1) MSSRWNRTIYVGNLPGDIRKCEVEDLFYKYGPIVDIDLKIPPRPPGYAFV
TaSRp30 (1) MSRRNGRTIYVGNLPEDIREREIEDLFCKYGPIVDIDLKIPPRPPVYAFV
TaSRp30a (1) MSRRWSRTIYVGNLPGDIREREVEDLFYKYGRIVEIDLKVPPRPPGFAFV
ZmSRP31 (1) MTRRNGCTIYVGNLPGDIREREVDDLFYKYGRIVEIDLKIPPRPPGFAFV
51 100
AtSR1 (51) EFDDARDAEDAIHGRDGYDFDGHRLRVELAHGGRRSS-DDTRGSFNGGGR
AtSRp30 (51) EFEDPRDADDAIYGRDGYDFDGCRLRVEIAHGGRRFSPSVDRYSSSYSAS
TaSRp30 (51) EFEDPRDADDAIYGRDGYDFDGCKLRVELAHGGKGPS-FDRPNSYTSSGR
TaSRp30a (51) EFEDPRDAEDAIHGRDGYNFDGNRLRVELAHGGRANS-SSLPNSYGGGGR
ZmSRP31 (51) EFEDARDAEDAIYGRDGYNFDGHRLRVELAHGGRGTSSFDRSSSYSSAGQ
101 150
AtSR1 (100) GGGRGRGDGRGDGGSRGPSRRSEFRVLVTGLPSSASWQDLKDHMRKGGDV
AtSRp30 (101) RAP------SRRSDYRVLVTGLPPSASWQDLKDHMRKAGDV
TaSRp30 (100) RG------ALRRSDYRVIVTGLPSSASWQDLKDHMRRAGDV
TaSRp30a (100) RGG------VSRHTEYRVLVTGLPSSASWQDLKDHMRKAGDV
ZmSRP31 (101) RG------ASKRSDYRVMVTGLPSSASWQDLKDHMRRAGDV
151 200
AtSR1 (150) CFSQVYRDARGTTGVVDYTCYEDMKYALKKLDDTEFRNAFSNGYVRVREY
AtSRp30 (136) CFSEVFPDRKGMSGVVDYSNYDDMKYAIRKLDATEFRNAFSSAYIRVREY
TaSRp30 (135) CFSDVYPGAGAITGIVEFPNYEDMKHAIRKLDDSEFRNAFSRTYIRVREY
TaSRp30a (136) CFSEVYREGGGTIGIVDYTNYDDMKYAIRKLDDTEFKNAFSRAPIRVKEY
ZmSRP31 (136) CFTDVYREAGATIGIADYTNYEDMKHAIRKLDDSEFRNAFSRTYVRVREY
201 250
AtSR1 (200) DSRKDSRSPSRGRSYSKSRSRSRGRSVSRSRS------RSRSRSRSPKA
AtSRp30 (186) ESRSVSRSP-DDSKSYRSRSRSRGPSCSYS------SKSRS-VS
TaSRp30 (185) NARGS------RSYSRSRSRSCSYSRSRS------HSYSRSRSPRS
TaSRp30a (186) AGKSS------RSYSRSRSRSRSGSYSRSPSPKKKPSRRSASRSRSRSV
ZmSRP31 (186) DARRS------RSRSRGRNRSKSRSRSHS------HSYSRSRS-CS
251 300
AtSR1 (243) KSSRRSPAKSTSRSPGPRSKSRSPSPRRSRSRSRSPLPSVQKEGSKSPSK
AtSRp30 (222) PARSISPRS------RPLSRSRSLYSSVSRSQSRSKSRSRSRSNSPVS
TaSRp30 (219) SSRSLSPAAP---A---RDKSASRSPIRSRSLPRSQSPVKSE------
TaSRp30a (229) SSHSRSPSKERSPS---RSPAKSRSPVAASPVVNGEAASPKRDPSKSPSR
ZmSRP31 (219) YSKSRSPRS------RSASESKSPVKASCTTETRKLWPCWTISIHVGY
301 315
AtSR1 (293) PSPAKSPIHTRSPSR
AtSRp30 (264) PVISG------
TaSRp30 (255) ------
TaSRp30a (276) SRSPDAKSE------
ZmSRP31 (261) IFVC------
Consensus positions: 69.5%; Identity positions: 60.0% (for TaSRp30 and AtSRp30)
TaTra2
MpTra2-like ACCESSION JC7299
AUTHORS Nishiyama,R., Yamato,K.T., Miura,K., Sakaida,M., Okada,S., Kono,K.,
Takahama,M., Sone,T., Takenaka,M., Fukuzawa,H. and Ohyama,K.
TITLE Comparison of expressed sequence tags from male and female sexual
organs of Marchantia polymorpha
JOURNAL DNA Res. 7 (3), 165-174 (2000)
PUBMED 10907846
NtTra2-like ACCESSION CAA70700
AUTHORS Petitot,A.S., Blein,J.P., Pugin,A. and Suty,L.
TITLE Cloning of two plant cDNAs encoding a beta-type proteasome subunit
and a transformer-2-like SR-related protein: early induction of the
corresponding genes in tobacco cells treated with cryptogein
JOURNAL Plant Mol. Biol. 35 (3), 261-269 (1997)
PUBMED 9349250
HsTra2 ACCESSION AAD19277
AUTHORS Beil,B., Screaton,G. and Stamm,S.
TITLE Molecular cloning of htra2-beta-1 and htra2-beta-2, two human
homologs of tra-2 generated by alternative splicing
JOURNAL DNA Cell Biol. 16 (6), 679-690 (1997)
PUBMED 9212162
DmTra2 ACCESSION P19018
AUTHORS Heinrichs,V., Ryner,L.C. and Baker,B.S.
TITLE Regulation of sex-specific selection of fruitless 5' splice sites
by transformer and transformer-2
JOURNAL Mol. Cell. Biol. 18 (1), 450-458 (1998)
PUBMED 9418892
1 50
DmTra2 (1) --MDREPLSSGRLHCSARYKHKRSASSSSAGTTSSGHKDRRSDYDYCGSR
HsTra2 (1) MSDSGEQNYGERESRSAS-RSGSAHGSGKSARHTPARSRSKEDSRRSRSK
MpTra2-like (1) ------SCFRVRECASLFPTYLVSQLSSWSVASIMADSPRHRSDRYSRSP
NtTra2-like (1) ------GFLVSWEMADSPRK---RYSRSP
TaTra2 (1) ---MSHSNEVRYTARSITPPADRDSGSKSPPPRRRAASKSPPPRRRAASK
51 100
DmTra2 (49) RHQ------RSSSRRRSRSRSSSESPPPEPRHRSGRS
HsTra2 (50) SRS------RSESRSRSRRSSRRHYTRSRSRSRSHRR
MpTra2-like (45) SPR------ERTERSRSRSPS---RRSPPPRERVE-R
NtTra2-like (21) SPW------EKNSRSKSPPES---YSRPRGRSRS--R
TaTra2 (48) SPPLPPPPPFPPMGVRTISRSPPPSSRRRSVSRSPPPKRRGRSRSRSRSR
101 150
DmTra2 (80) SRDRERMHK------SREHPQASRCIGVFGLNTNTS
HsTra2 (81) SRSRSYSRDYRRRHSHSHSPMSTRRRHVGNRANPDPNCCLGVFGLSLYTT
MpTra2-like (72) SRSRSPSR------RFRHDALNPGN--NLYVTGLSTRVN
NtTra2-like (47) SRSRSRSRS------RGRGEVSNPGN--TLYVTGLSTRVT
TaTra2 (98) NRSRSRSRD------KDDVRNPGN--NLYVTGLSTRTQ
151 200
DmTra2 (110) QHKVRELFNKYGPIERIQMVIDAQTQRSRGFCFIYFEKLSDARAAKDSCS
HsTra2 (131) ERDLREVFSKYGPIADVSIVYDQQSRRSRGFAFVYFENVDDAKEAKERAN
MpTra2-like (103) EKDLEEHFSREGKVLECRLVLDPRTRESRGFGFVTMEHLEGAERCIKYLN
NtTra2-like (79) ERDLEEHFSKEGKVKSVFLVVEPRSRISRGFAFITMDSLEDANRCIKHLN
TaTra2 (128) ETDLEKFFSKEGKVKDCRVVIDPRTKESRDFAFVTMENVEDARRCIKYLH
201 250
DmTra2 (160) GIEVDGRRIRVDFSITQRAHTPTPGVYLGRQPRGKAPRSFSPRRGRRVYH
HsTra2 (181) GMELDGRRIRVDFSITKRPHTPTPGIYMGRPTYGSSRRRDYYDRGYDRGY
MpTra2-like (153) RSTLEGRMITVEKAKRKRARTPTPGEYLGVRAMQSNRR---GGRGGGFRR
NtTra2-like (129) QSVLEGRYITVEKSRRKRARTPTPGHYLGLKNARGEGR---GDRGRYRDR
TaTra2 (178) RTVLEGRLISVAKAKRTRERTPTPGEYCGPRGGRSRVE---PRRSRSPRR
251 300
DmTra2 (210) DRSASPYDNYRDRYDYRN-----DRYDRNLRR-SPSRNRYTR------
HsTra2 (231) DDRDYYSRSYRGGGGGGGGWRAAQDRDQIYRRRSPSPYYSRG---G----
MpTra2-like (200) DARNSRHSPEYAPYSGG------RDRSPRY-SPYRGHQR------
NtTra2-like (176) EDYGYRRSPRHSPYRSR------RDYSPRR-SPYGEGQEGSVLGRILL
TaTra2 (225) SSRSSRDRS-RSPAARRD------RDSRDRKRD------
301 328
DmTra2 (246) ------NRSYSRSRSPQLRRTSSRY
HsTra2 (274) ------YRSRSRSRSYSPRRY------
MpTra2-like (232) ------DRSSSPQYAPYRR------
NtTra2-like (217) MQEAMLVVQDRSLSSPMNS------
TaTra2 (251) ------
Consensus positions: 26.9%; Identity positions: 21.7% (for TaTra2 and DmTra2)
TaTRN-SR
HsTRN-SR ACCESSION Q9Y5L0
AUTHORS Lai,M.C., Lin,R.I. and Tarn,W.Y.
TITLE Transportin-SR2 mediates nuclear import of phosphorylated SR
proteins
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 98 (18), 10154-10159 (2001)
PUBMED 11517331
ScMtr10p ACCESSION NP_014803
AUTHORS Dujon,B., Albermann,K., Aldea,M., Alexandraki,D., Ansorge,W.,
Arino,J., Benes,V., Bohn,C., Bolotin-Fukuhara,M., Bordonne,R.,
Boyer,J., Camasses,A., Casamayor,A., Casas,C., Cheret,G.,
Cziepluch,C., Daignan-Fornier,B., Dang,D.V., de Haan,M., Delius,H.,
Durand,P., Fairhead,C., Feldmann,H., Gaillon,L., Kleine,K. et al.
TITLE The nucleotide sequence of Saccharomyces cerevisiae chromosome XV
JOURNAL Nature 387 (6632 Suppl), 98-102 (1997)
PUBMED 9169874
1 50
HsTRN-SR (1) MEGAKPTLQLVYQAVQALYHDPDPSGKERASFWLGELQRSVHAWEISDQL
TaTRN-SR (1) --MEAQATATVKEALAALYHHPDDAIRAAADRWLQKFQHTLDAWQVADSL
ScMtr10p (1) --MDNLQVSDIETALQCISSTASQDDKNKALQFLEQFQRSTVAWSICNEI
51 100
HsTRN-SR (51) LQIR----QDVESCYFAAQTMKMKIQTSFYELPTDSHASLRDSLLTHIQN
TaTRN-SR (49) LHDES---SNLETLMFCSQTLRSKVQRDFEELPSEAFRPLQDSLYGLLKK
ScMtr10p (49) LSKEDPTNALLELNIFAAQTLRNKVTYDLSQL-ENNLPQFKDSLLTLLLS
101 150
HsTRN-SR (97) LKDLSPVIVTQLALAIADLALQMPSWKG----CVQTLVEKYSNDVTSLPF
TaTRN-SR (96) FNKGPPKVRTQICIAIAALAVHVPVEDWGGGGIVDWLGDEMKSQQEFIPS
ScMtr10p (98) HNQ--KLIITQLNVALARLAIQFLEWQN------PIFEIISLLNSSPSI
151 200
HsTRN-SR (143) LLEILTVLPEEVHSRSLRIGANRR--TEIIEDLAFYSSTVVSLLMTCVEK
TaTRN-SR (146) FLELLIILPQETSSYRIAARPERR--NQFENDLCSSANVALSLLTACLGF
ScMtr10p (139) LLNFLRILPEETLDIASTSLTEVEFNSRIHELIDPIAEDVLKFLVSCIDL
201 250
HsTRN-SR (191) ------AGTDEKMLMKVFRCLGSWFNLGVLDSNFMANNKLLALLFEVLQQ
TaTRN-SR (194) ------DELKEQVLEGFASWLR--FCHGITAATLASHPLVHTALSSLNTD
ScMtr10p (189) LQNTDGNSSSSISLEQILRCLNSWSYEFPVEQLLTVQPLINLVFETISNG
251 300
HsTRN-SR (235) DKTSSNLHEAASDCVCSALYAIENVETNLPLAMQLFQGVLTLET------
TaTRN-SR (236) QFLEAAVNVTSELIHFTVSRDSCGITEQFPLIQILIPHVMGLK------
ScMtr10p (239) NESDMEAFDSAIDCLCVILRESRDTTNEQLISALFHQLMLLQEKLLPTLF
301 350
HsTRN-SR (279) AYHMAVAREDLDKVLNYCRIFTELCETFLEKIVCTPGQGLGDLRTLELLL
TaTRN-SR (279) -EQLKDSSKDEEDVKAIARLFADMGDSYADLIATGSG---DAMQIVNALL
ScMtr10p (289) TDHPLNDEYDDDLLEGMTRLFVEAGEAWSVVISKNPD--FFKPMVLVLLM
351 400
HsTRN-SR (329) ICAGHPQYEVVEISFNFWYRLGEHLYKTNDEVIHG---IFKAYIQR----
TaTRN-SR (325) EVTSHSEFDISSMTFNFWHHLKRNLTVRDSYTSCGSEVSIEAERNRRMQL
ScMtr10p (337) LTCKNEDLDVVSYTFPFWFNFKQSLVLPRYQESRKAYSDIFVKLIN----
401 450
HsTRN-SR (372) ------LLHALARHCQLEPDHEGVPEETDDFGEFRMRVSDLVKDLIFL
TaTRN-SR (375) FRPPFEVLVSLVSSRVEYPEDYHTFSEEDRRDFRYARYAVSDVLLDATDV
ScMtr10p (383) ------GIITHLQYPSGQFSSKEEEDKFKDFRYHMGDVLKDCTAV
451 500
HsTRN-SR (414) IGSMECFAQLYSTLK------EG-NPPWEVTEAVLFIMAAIAKSVDPKKP
TaTRN-SR (425) LGGDSTLKILFMKLIQACGSGAEQNQNWQPLEAALFCIQAIAKSLSIEE-
ScMtr10p (422) VGTSEALSQPLIRIKSA----IENNNSWQIMEAPLFSLRTMAKEISLTE-
501 550
HsTRN-SR (457) FSNAACHHSLLFGQNITSEISNCEYLPPVLRENNPTLVEVLEGVVRLPET
TaTRN-SR (474) ------KEILPQVMPLLPRFPHQEQL
ScMtr10p (467) ------NTILPEIIKIICNLPEQAKI
551 600
HsTRN-SR (507) VHTAVRYTSIELVGEMSEVVDRNPQFLDPVLGYLMKGLCEKP------L
TaTRN-SR (494) LQTVC--STIGAFSKWIDAAPAELPILPPLVDILNKGMSTSED------T
ScMtr10p (487) RYAST---LVLGRYTEWTAKH--PELLEVQLQYIFNGFQLHEGSSDMQSI
601 650
HsTRN-SR (550) ASAAAKAIHNICSVCRDHMAQHFNGLLEIARSLDS----FLLSPEAAVGL
TaTRN-SR (536) AAAASVAFKYICEDCRGKFSGSLDGLFQIYHVAISGVGGYKVSSEDSLHL
ScMtr10p (532) ITASSHALMFFCSDCSKLLVGYIDQLINFFLNVQS-----SIDIESQFEL
651 700
HsTRN-SR (596) LKGTALVLARLPLDKITECLSELCSVQVMALKKLLSQ---EPSNGISSDP
TaTRN-SR (586) VEALSVVITTLPQDHARRALELICMPIINSLQEIIQQGESAPQQVPARHL
ScMtr10p (577) CQGLSAVINNQPEAKVSVIFQKLVDDNLRQIEALIPQWK-ANPTLLAPQI
701 750
HsTRN-SR (643) TVFLDRLAVIFRHTNPIVE--NGQTHPCQKVIQEIWPVLSETLNKH--RA
TaTRN-SR (636) TVHIDRLSTIFSNVK------LPEVVAEAVNRYWSTLKIIFDHR--AW
ScMtr10p (626) ADKIDLLYALFEELKPRYNYPQQGSEPLLPRIEFIWKALRTLLVDAGAMT
751 800
HsTRN-SR (689) DNRIVERCCRCLRFAVRCVGKGSAALLQPLVTQMVNVYHVHQHSCFLYLG
TaTRN-SR (676) DTRTMESLCRSCKFAVRTCGRSMGITIGAMLLEIQTLYQQHNQSCFLYLS
ScMtr10p (676) DSIIVERVAKLLRRIFERFHVFCEPILPSVAEFLIQGYLTTGFGSYLWCS
801 850
HsTRN-SR (739) SILVDEYGMEEG------CRQGLLDMLQALCIPTFQLLEQQNGLQNH
TaTRN-SR (726) SEVIKIFGSDPS------CASYLTCLIQTLFNHTIQLLRTIQDFTAR
ScMtr10p (726) GSLIVIFGDDESFPISPSLKDAVWKFALSQCETFILNFNKFDKLQLNDYH
851 900
HsTRN-SR (780) PDTVDDLFRLATRFIQRSPVTLLRSQVVIPILQWAIASTT-LDHRDANCS
TaTRN-SR (767) PDIADDCFLLASRCIRYCPDLFVPTEIFPRLVDCAMAGVT-IQHREACKS
ScMtr10p (776) EAIIDFFSLISDLIMFYPGAFLNSTELLGPVLNVALECVNKLDNYDAYIC
901 950
HsTRN-SR (829) VMRFLRDLIHTGVAND------HEEDFELRKELIGQVMNQLGQQLVSQL
TaTRN-SR (816) ILCFLSDTFDLAKS------PEGEKYRDLINTIVLQRGATLARIM
ScMtr10p (826) ILRCLDDIISWGFKTPPISTVSIEIVPDEWRKQVINEVVIAHGNQLILVL
951 1000
HsTRN-SR (872) LHTCCFCLPPYTLPDVAEVLWEIMQVDRPTFCRWLENSLKGLPKETTVGA
TaTRN-SR (855) IASLTGALPSGRLEEASYVLLSLNRAFGGNMLNWTRDCIALIPPQALTDS