LOCUS Exported 3776 bp ds-DNA circular SYN 12-JUN-2014
DEFINITION .
ACCESSION .
VERSION .
KEYWORDS pCMV Flag-C
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 3776)
AUTHORS Trial User
TITLE Direct Submission
JOURNAL Exported Thursday, Jun 12, 2014 from SnapGene 2.4.0
FEATURES Location/Qualifiers
source 1..3776
/organism="synthetic DNA construct"
/mol_type="other DNA"
enhancer 27..330
/note="CMV enhancer"
/note="human cytomegalovirus immediate early enhancer"
promoter 331..534
/note="CMV promoter"
/note="human cytomegalovirus (CMV) immediate early
promoter"
intron 672..768
/note="SV40 intron"
/note="modified SV40 intron with splice donor and acceptor
sites"
gap 838..847
/estimated_length="10"
misc_feature 838..847
/note="ORF insert"
CDS 877..900
/codon_start=1
/product="FLAG epitope tag, followed by an enterokinase
cleavage site"
/note="FLAG tag"
/translation="DYKDDDDK"
polyA_signal 915..1049
/note="SV40 poly(A) signal"
/note="SV40 polyadenylation signal"
primer_bind complement(1160..1176)
/note="M13 rev"
/note="common sequencing primer, one of multiple similar
variants"
rep_origin complement(1562..2150)
/direction=LEFT
/note="ori"
/note="high-copy-number colE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(2321..3181)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(3182..3286)
/gene="bla"
/note="AmpR promoter"
primer_bind 3760..3776
/note="M13 fwd"
/note="common sequencing primer, one of multiple similar
variants"
ORIGIN
1 gagttcgagcttgcatgcctgcaggtcgttacataacttacggtaaatggcccgcctggc
61 tgaccgcccaacgacccccgcccattgacgtcaataatgacgtatgttcccatagtaacg
121 ccaatagggactttccattgacgtcaatgggtggagtatttacggtaaactgcccacttg
181 gcagtacatcaagtgtatcatatgccaagtacgccccctattgacgtcaatgacggtaaa
241 tggcccgcctggcattatgcccagtacatgaccttatgggactttcctacttggcagtac
301 atctacgtattagtcatcgctattaccatggtgatgcggttttggcagtacatcaatggg
361 cgtggatagcggtttgactcacggggatttccaagtctccaccccattgacgtcaatggg
421 agtttgttttggcaccaaaatcaacgggactttccaaaatgtcgtaacaactccgcccca
481 ttgacgcaaatgggcggtaggcgtgtacggtgggaggtctatataagcagagctcgttta
541 gtgaaccgtcagatcgcctggagacgccatccacgctgttttgacctccatagaagacac
601 cgggaccgatccagcctccggactctagaggatccggtactagaggaactgaaaaaccag
661 aaagttaactggtaagtttagtctttttgtcttttatttcaggtcccggatccggtggtg
721gtgcaaatcaaagaactgctcctcagtggatgttgcctttacttctaggcctgtacggaa
781 gtgttacttctgctctaaaagctgcggaattgtacccgcgggcccaggcccgaattcnnn
841 nnnnnnnggtcgaccgagatctctcgaggtaccggcgactacaaggacgacgatgacaag
901 taagcggccgcggggatccagacatgataagatacattgatgagtttggacaaaccacaa
961 ctagaatgcagtgaaaaaaatgctttatttgtgaaatttgtgatgctattgctttatttg
1021 taaccattataagctgcaataaacaagttaacaacaacaattgcattcattttatgtttc
1081 aggttcagggggaggtgtgggaggttttttcggatcctctagagtcgatctgcaggcatg
1141 ctagcttggcgtaatcatggtcatagctgtttcctgtgtgaaattgttatccgctcacaa
1201 ttccacacaacatacgagccggaagcataaagtgtaaagcctggggtgcctaatgagtga
1261 gctaactcacattaattgcgttgcgctcactgcccgctttccagtcgggaaacctgtcgt
1321 gccagctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgct
1381 cttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtat
1441 cagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaaga
1501 acatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgt
1561 ttttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggt
1621 ggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgc
1681 gctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaa
1741 gcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgct
1801 ccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggta
1861 actatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactg
1921 gtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggc
1981 ctaactacggctacactagaaggacagtatttggtatctgcgctctgctgaagccagtta
2041 ccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtg
2101 gtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctt
2161 tgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttgg
2221 tcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagtttta
2281 aatcaatctaaagtatatatgagtaaacttggtctgacagttaccaatgcttaatcagtg
2341 aggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcg
2401 tgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgc
2461 gagacccacgctcaccggctccagatttatcagcaataaaccagccagccggaagggccg
2521 agcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccggg
2581 aagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgccattgctacag
2641 gcatcgtggtgtcacgctcgtcgtttggtatggcttcattcagctccggttcccaacgat
2701 caaggcgagttacatgatcccccatgttgtgcaaaaaagcggttagctccttcggtcctc
2761 cgatcgttgtcagaagtaagttggccgcagtgttatcactcatggttatggcagcactgc
2821 ataattctcttactgtcatgccatccgtaagatgcttttctgtgactggtgagtactcaa
2881 ccaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatac
2941 gggataataccgcgccacatagcagaactttaaaagtgctcatcattggaaaacgttctt
3001 cggggcgaaaactctcaaggatcttaccgctgttgagatccagttcgatgtaacccactc
3061 gtgcacccaactgatcttcagcatcttttactttcaccagcgtttctgggtgagcaaaaa
3121 caggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaatgttgaatactca
3181 tactcttcctttttcaatattattgaagcatttatcagggttattgtctcatgagcggat
3241 acatatttgaatgtatttagaaaaataaacaaataggggttccgcgcacatttccccgaa
3301 aagtgccacctgacgtctaagaaaccattattatcatgacattaacctataaaaataggc
3361 gtatcacgaggccctttcgtctcgcgcgtttcggtgatgacggtgaaaacctctgacaca
3421 tgcagctcccggagacggtcacagcttgtctgtaagcggatgccgggagcagacaagccc
3481 gtcagggcgcgtcagcgggtgttggcgggtgtcggggctggcttaactatgcggcatcag
3541 agcagattgtactgagagtgcaccatatgcggtgtgaaataccgcacagatgcgtaagga
3601 gaaaataccgcatcaggcgccattcgccattcaggctgcgcaactgttgggaagggcgat
3661 cggtgcgggcctcttcgctattacgccagctggcgaaagggggatgtgctgcaaggcgat
3721 taagttgggtaacgccagggttttcccagtcacgacgttgtaaaacgacggccagt
//