Supplementary Table 1 Sample details with each sample number, species, sampling location, sex, B- chromosome (when observed), mtDNA haplotype number, Cytb Genbank accession number, 16S Genbank accession number, nuDNA haplotype number, tyrosinase Genbank accession number (Allele A and B), Geographical coordinates of the sampling location

Species / Location / Sample number / Sex / Bchr / mt DNA H / Acc.#Cytb / Acc.#16S / nu DNA H / Acc.#Tyr A / Acc.#Tyr B / Coordinates
L. hochstetteri / Brynderwyn / 3005 / F / 1 / 29 / FJ950380 / FJ950486 / NA / NA / NA / 36 5.778S,174 25.801E
L. hochstetteri / Brynderwyn / 3006 / M / 0 / 29 / FJ950381 / FJ950487 / NA / NA / NA / 36 5.778S,174 25.801E
L. hochstetteri / Brynderwyn / 3007 / F / 1 / 29 / FJ950382 / FJ950488 / 1,1 / FJ950246 / FJ950247 / 36 5.778S,174 25.801E
L. hochstetteri / Golden Cross / 3029 / F / 1 / 22 / FJ950388 / FJ950494 / 11,11 / FJ950258 / FJ950259 / 37 20.416S,175 46.466E
L. hochstetteri / Golden Cross / 3030 / M / 0 / 23 / FJ950389 / FJ950495 / 1,1 / FJ950260 / FJ950261 / 37 20.416S,175 46.466E
L. hochstetteri / Golden Cross / 3041 / 24 / FJ950397 / FJ950503 / NA / NA / NA / 37 20.416S,175 46.466E
L. hochstetteri / Golden Cross / 4224 / F / 1 / 25 / FJ950406 / FJ950512 / 11,11 / FJ950282 / FJ950283 / 37 20.416S,175 46.466E
L. hochstetteri / Golden Cross / 4225 / M / 0 / 26 / FJ950407 / FJ950513 / 11,12 / FJ950284 / FJ950285 / 37 20.416S,175 46.466E
L. hochstetteri / Golden Cross / 4226 / F / 1 / 27 / FJ950408 / FJ950514 / 12,12 / FJ950286 / FJ950287 / 37 20.416S,175 46.466E
L. hochstetteri / Great Barrier Is. / 2999 / F / 0 / 43 / FJ950376 / FJ950482 / 1,1 / FJ950240 / FJ950241 / 36 4.793S,175 22.806E
L. hochstetteri / Great Barrier Is. / 3000 / F / 0 / 43 / FJ950377 / FJ950483 / 1,1 / FJ950242 / FJ950243 / 36 4.793S,175 22.806E
L. hochstetteri / Great Barrier Is. / 3001 / F / 0 / 41 / FJ950378 / FJ950484 / NA / NA / NA / 36 4.793S,175 22.806E
L. hochstetteri / Great Barrier Is. / 3002 / M / 0 / 42 / FJ950379 / FJ950485 / 1,1 / FJ950244 / FJ950245 / 36 4.793S,175 22.806E
L. hochstetteri / Great Barrier Is. / 3031 / F / 0 / 42 / FJ950390 / FJ950496 / 1,8 / FJ950262 / FJ950263 / 36 4.793S,175 22.806E
L. hochstetteri / Great Barrier Is. / 3032 / F / 0 / 42 / FJ950391 / FJ950497 / NA / NA / NA / 36 4.793S,175 22.806E
L. hochstetteri / Hunua Mountains / 2121 / F / 4 / 36 / FJ950357 / FJ950463 / NA / NA / NA / 37 2.305S,175 12.376E
L. hochstetteri / Hunua Mountains / 2122 / M / 0 / 36 / FJ950358 / FJ950464 / 4,4 / FJ950214 / FJ950215 / 37 2.305S,175 12.376E
L. hochstetteri / Hunua Mountains / 2152 / F / 2 / 36 / FJ950363 / FJ950469 / 22,22 / FJ950222 / FJ950223 / 37 2.305S,175 12.376E
L. hochstetteri / Hunua Mountains / 3024 / M / - / 36 / FJ950386 / FJ950492 / 5,6 / FJ950254 / FJ950255 / 37 2.305S,175 12.376E
L. hochstetteri / Hunua Mountains / 3025 / F / - / 36 / FJ950387 / FJ950493 / 25,25 / FJ950256 / FJ950257 / 37 2.305S,175 12.376E
L. hochstetteri / Manganuku / 1000 / 9 / FJ950322 / FJ950428 / 1,1 / FJ950150 / FJ950151 / 38 16.720S,177 23.202E
L. hochstetteri / Manganuku / 1001 / 9 / FJ950323 / FJ950429 / 1,1 / FJ950152 / FJ950153 / 38 16.720S,177 23.202E
L. hochstetteri / Manganuku / 1002 / 9 / FJ950324 / FJ950430 / 1,1 / FJ950154 / FJ950155 / 38 16.720S,177 23.202E
L. hochstetteri / Manganuku / 1003 / 8 / FJ950325 / FJ950431 / 1,1 / FJ950156 / FJ950157 / 38 16.720S,177 23.202E
L. hochstetteri / Manganuku / 1004 / 6 / FJ950326 / FJ950432 / NA / NA / NA / 38 16.720S,177 23.202E
L. hochstetteri / Manganuku / 1005 / - / - / 10 / FJ950327 / FJ950433 / 1,1 / FJ950158 / FJ950159 / 38 16.720S,177 23.202E
L. hochstetteri / Manganuku / 1006 / - / - / 10 / FJ950328 / FJ950434 / 1,1 / FJ950160 / FJ950161 / 38 16.720S,177 23.202E
L. hochstetteri / Mt Maungatautari / MMT / 33 / FJ950415 / FJ950521 / NA / NA / NA / 38 1.464S,175 34.454E
L. hochstetteri / Mt. Moehau / 2124 / F / 13 / 37 / FJ950359 / FJ950465 / 10,21 / FJ950216 / FJ950217 / 36 32.408S,175 24.118E
L. hochstetteri / Mt. Moehau / 2208 / F / 12 / 37 / FJ950365 / FJ950471 / 11,11 / FJ950226 / FJ950227 / 36 32.408S,175 24.118E
L. hochstetteri / Mt. Moehau / 2995 / F / 12 / 37 / FJ950375 / FJ950481 / NA / NA / NA / 36 32.408S,175 24.118E
L. hochstetteri / Mt. Moehau / 5132 / M / 15 / 37 / FJ950410 / FJ950516 / 10,21 / FJ950290 / FJ950291 / 36 32.408S,175 24.118E
L. hochstetteri / Mt. Moehau / 5186 / F / - / 38 / FJ950413 / FJ950519 / 7,7 / FJ950296 / FJ950297 / 36 32.408S,175 24.118E
L. hochstetteri / Mt. Ranginui / 3034 / F / 1 / 33 / FJ950392 / FJ950498 / 1,1 / FJ950264 / FJ950265 / 38 21.932S,175 28.650E
L. hochstetteri / Mt. Ranginui / 3050 / M / - / 33 / FJ950399 / FJ950505 / NA / NA / NA / 38 21.932S,175 28.650E
L. hochstetteri / Mt. Ranginui / 3056 / F / 1 / 33 / FJ950400 / FJ950506 / 1,1 / FJ950274 / FJ950275 / 38 21.932S,175 28.650E
L. hochstetteri / Mt. Ranginui / 3064 / F / 1 / 32 / FJ950401 / FJ950507 / 1,8 / FJ950276 / FJ950277 / 38 21.932S,175 28.650E
L. hochstetteri / North Kaimai / NK1 / 21 / FJ950416 / FJ950522 / 11,12 / FJ950300 / FJ950301 / 37 29.128S,175 45.915E
L. hochstetteri / North Kaimai / NK2 / 19 / FJ950417 / FJ950523 / 11,12 / FJ950302 / FJ950303 / 37 29.128S,175 45.915E
L. hochstetteri / North Kaimai / NK3 / 18 / FJ950418 / FJ950524 / 11,12 / FJ950304 / FJ950305 / 37 29.128S,175 45.915E
L. hochstetteri / Otawa / OT1 / 35 / FJ950419 / FJ950525 / 11,11 / FJ950306 / FJ950307 / 37 49.538S,176 14.116E
L. hochstetteri / Otawa / OT2 / 35 / FJ950420 / FJ950526 / 11,11 / FJ950308 / FJ950309 / 37 49.538S,176 14.116E
L. hochstetteri / Otawa / OT3 / 35 / FJ950421 / FJ950527 / 11,11 / FJ950310 / FJ950311 / 37 49.538S,176 14.116E
L. hochstetteri / Otawa / OT4 / 35 / FJ950422 / FJ950528 / 11,11 / FJ950312 / FJ950313 / 37 49.538S,176 14.116E
L. hochstetteri / Ruatoria / 1030 / - / - / 4 / FJ950334 / FJ950440 / 1,1 / FJ950172 / FJ950173 / 37 49.490S,178 3.841E
L. hochstetteri / Ruatoria / 1031 / 5 / FJ950335 / FJ950441 / 1,1 / FJ950174 / FJ950175 / 37 49.490S,178 3.841E
L. hochstetteri / Ruatoria / 1032 / 1 / FJ950336 / FJ950442 / NA / NA / NA / 37 49.490S,178 3.841E
L. hochstetteri / Ruatoria / 1033 / 1 / FJ950337 / FJ950443 / 1,1 / FJ950176 / FJ950177 / 37 49.490S,178 3.841E
L. hochstetteri / Ruatoria / 1035 / 1 / FJ950338 / FJ950444 / 1,1 / FJ950178 / FJ950179 / 37 49.490S,178 3.841E
L. hochstetteri / Ruatoria / 1036 / 1 / FJ950339 / FJ950445 / NA / NA / NA / 37 49.490S,178 3.841E
L. hochstetteri / Ruatoria / 1037 / 1 / FJ950340 / FJ950446 / 1,1 / FJ950180 / FJ950181 / 37 49.490S,178 3.841E
L. hochstetteri / Ruatoria / 1038 / 1 / FJ950341 / FJ950447 / 1,1 / FJ950182 / FJ950183 / 37 49.490S,178 3.841E
L. hochstetteri / Ruatoria / 1039 / 1 / FJ950342 / FJ950448 / 1,1 / FJ950184 / FJ950185 / 37 49.490S,178 3.841E
L. hochstetteri / Ruatoria / 1040 / 1 / FJ950343 / FJ950449 / 1,1 / FJ950186 / FJ950187 / 37 49.490S,178 3.841E
L. hochstetteri / Ruatoria / 1050 / 2 / FJ950344 / FJ950450 / 1,1 / FJ950188 / FJ950189 / 37 49.490S,178 3.841E
L. hochstetteri / Ruatoria / 1051 / 1 / FJ950345 / FJ950451 / 20,2 / FJ950190 / FJ950191 / 37 49.490S,178 3.841E
L. hochstetteri / Ruatoria / 1052 / - / - / 3 / FJ950346 / FJ950452 / 1,1 / FJ950192 / FJ950193 / 37 49.490S,178 3.841E
L. hochstetteri / Ruatoria / 1053 / - / - / 2 / FJ950347 / FJ950453 / 1,1 / FJ950194 / FJ950195 / 37 49.490S,178 3.841E
L. hochstetteri / Ruatoria / 1054 / - / - / 2 / FJ950348 / FJ950454 / 1,1 / FJ950196 / FJ950197 / 37 49.490S,178 3.841E
L. hochstetteri / South Kaimai / SK1 / 20 / FJ950423 / FJ950529 / 11,11 / FJ950314 / FJ950315 / 37 41.652S,175 54.882E
L. hochstetteri / South Kaimai / SK2 / 20 / FJ950424 / FJ950530 / 11,11 / FJ950316 / FJ950317 / 37 41.652S,175 54.882E
L. hochstetteri / South Kaimai / SK3 / 20 / FJ950425 / FJ950531 / 11,11 / FJ950318 / FJ950319 / 37 41.652S,175 54.882E
L. hochstetteri / Tapu / 2109 / F / 7 / 17 / FJ950352 / FJ950458 / 23,24 / FJ950204 / FJ950205 / 36 58.723S,175 30.944E
L. hochstetteri / Tapu / 2110 / 16 / FJ950353 / FJ950459 / 13,13 / FJ950206 / FJ950207 / 36 54.946S,174 31.146E
L. hochstetteri / Tapu / 2111 / 14 / FJ950354 / FJ950460 / 13,17 / FJ950208 / FJ950209 / 36 54.946S,174 31.146E
L. hochstetteri / Tapu / 2210 / F / 6 / 16 / FJ950367 / FJ950473 / NA / NA / NA / 36 58.723S,175 30.944E
L. hochstetteri / Tapu / 2230 / F / 13 / 15 / FJ950371 / FJ950477 / 18,19 / FJ950234 / FJ950235 / 36 58.723S,175 30.944E
L. hochstetteri / Toatoa / 2103 / F / 1 / 6 / FJ950349 / FJ950455 / 2,3 / FJ950198 / FJ950199 / 38 7.392S,177 31.372E
L. hochstetteri / Toatoa / 2106 / 6 / FJ950350 / FJ950456 / 1,1 / FJ950200 / FJ950201 / 38 7.392S,177 31.372E
L. hochstetteri / Toatoa / 2269 / 6 / FJ950372 / FJ950478 / 2,3 / FJ950236 / FJ950237 / 38 7.392S,177 31.372E
L. hochstetteri / Toatoa / 3490 / F / 1 / 6 / FJ950403 / FJ950509 / 1,1 / FJ950278 / FJ950279 / 38 7.392S,177 31.372E
L. hochstetteri / Toatoa / 4208 / 6 / FJ950405 / FJ950511 / NA / NA / NA / 38 7.392S,177 31.372E
L. hochstetteri / Tokatea Ridge / 2108 / F / 6 / 39 / FJ950351 / FJ950457 / 7,7 / FJ950202 / FJ950203 / 36 42.984S,175 30.751E
L. hochstetteri / Tokatea Ridge / 2112 / M / 5 / 40 / FJ950355 / FJ950461 / 7,7 / FJ950210 / FJ950211 / 36 42.984S,175 30.751E
L. hochstetteri / Tokatea Ridge / 2117 / F / 5 / 39 / FJ950356 / FJ950462 / 7,7 / FJ950212 / FJ950213 / 36 42.984S,175 30.751E
L. hochstetteri / Tokatea Ridge / 2209 / 39 / FJ950366 / FJ950472 / 4,4 / FJ950228 / FJ950229 / 36 42.984S,175 30.751E
L. hochstetteri / Tokatea Ridge / 2272 / F / 4 / 39 / FJ950373 / FJ950479 / NA / NA / NA / 36 42.984S,175 30.751E
L. hochstetteri / Tokatea Ridge / 3779 / - / - / 39 / FJ950404 / FJ950510 / 4,7 / FJ950280 / FJ950281 / 36 42.984S,175 30.751E
L. hochstetteri / Waipu / 3035 / F / 1 / 29 / FJ950393 / FJ950499 / 9,1 / FJ950266 / FJ950267 / 35 59.206S,174 27.868E
L. hochstetteri / Waipu / 3036 / M / 0 / 30 / FJ950394 / FJ950500 / 15,16 / FJ950268 / FJ950269 / 35 59.206S,174 27.868E
L. hochstetteri / Waipu / 3048 / M / 0 / 31 / FJ950398 / FJ950504 / 14,14 / FJ950272 / FJ950273 / 35 59.206S,174 27.868E
L. hochstetteri / Waitakere Mountains / 3018 / F / 2 / 11 / FJ950383 / FJ950489 / 1,1 / FJ950248 / FJ950249 / 36 54.946S,174 31.146E
L. hochstetteri / Waitakere Mountains / 3020 / F / 4 / 13 / FJ950384 / FJ950490 / 1,1 / FJ950250 / FJ950251 / 36 54.946S,174 31.146E
L. hochstetteri / Waitakere Mountains / 3021 / F / 5 / 13 / FJ950385 / FJ950491 / 1,1 / FJ950252 / FJ950253 / 36 54.946S,174 31.146E
L. hochstetteri / Waitakere Mountains / 3037 / F / - / 11 / FJ950395 / FJ950501 / 1,1 / FJ950270 / FJ950271 / 36 54.946S,174 31.146E
L. hochstetteri / Waitakere Mountains / 5127 / F / - / 12 / FJ950409 / FJ950515 / 1,1 / FJ950288 / FJ950289 / 36 54.946S,174 31.146E
L. hochstetteri / Warkworth / 2143 / M / 0 / 31 / FJ950360 / FJ950466 / 14,14 / FJ950218 / FJ950219 / 36 26.327S,174 39.171E
L. hochstetteri / Warkworth / 2144 / F / 1 / 31 / FJ950361 / FJ950467 / 14,14 / FJ950220 / FJ950221 / 36 26.327S,174 39.171E
L. hochstetteri / Warkworth / 2154 / 31 / FJ950364 / FJ950470 / 14,14 / FJ950224 / FJ950225 / 36 26.327S,174 39.171E
L. hochstetteri / Warkworth / 2214 / 31 / FJ950369 / FJ950475 / NA / NA / NA / 36 26.327S,174 39.171E
L. hochstetteri / Warkworth / 2229 / 28 / FJ950370 / FJ950476 / 14,14 / FJ950232 / FJ950233 / 36 26.327S,174 39.171E
L. hochstetteri / Whanarua / 1011 / - / - / 1 / FJ950329 / FJ950435 / 1,1 / FJ950162 / FJ950163 / 37 41.128S,177 47.172E
L. hochstetteri / Whanarua / 1012 / 4 / FJ950330 / FJ950436 / 1,1 / FJ950164 / FJ950165 / 37 41.128S,177 47.172E
L. hochstetteri / Whanarua / 1013 / - / - / 1 / FJ950331 / FJ950437 / 1,1 / FJ950166 / FJ950167 / 37 41.128S,177 47.172E
L. hochstetteri / Whanarua / 1015 / - / - / 4 / FJ950332 / FJ950438 / 1,1 / FJ950170 / FJ950171 / 37 41.128S,177 47.172E
L. hochstetteri / Whanarua / 3040 / 1 / FJ950333 / FJ950439 / NA / NA / NA / 37 41.128S,177 47.172E
L. hochstetteri / Whanarua / 3075 / F / 1 / 1 / FJ950396 / FJ950502 / NA / NA / NA / 37 41.128S,177 47.172E
L. hochstetteri / Whanarua / 1014 / 1 / FJ950402 / FJ950508 / 1,1 / FJ950168 / FJ950169 / 37 41.128S,177 47.172E
L. hochstetteri / Whareorino / 27 / - / - / 34 / FJ950374 / FJ950480 / 8,8 / FJ950238 / FJ950239 / 38 24.707S,174 41.490E
L. hochstetteri / Whareorino / 69 / - / - / 34 / FJ950411 / FJ950517 / 1,1 / FJ950292 / FJ950293 / 38 24.707S,174 41.490E
L. hochstetteri / Whareorino / 5183 / M / 0 / 34 / FJ950412 / FJ950518 / 1,8 / FJ950294 / FJ950295 / 38 24.707S,174 41.490E
L. hochstetteri / Whareorino / 5185 / M / 0 / 34 / FJ950414 / FJ950520 / 8,8 / FJ950298 / FJ950299 / 38 24.707S,174 41.490E
L. hochstetteri / Whitikau / 2146 / M / 0 / 7 / FJ950362 / FJ950468 / NA / NA / NA / 38 8.807S,177 30.098E
L. hochstetteri / Whitikau / 2213 / M / 0 / 7 / FJ950368 / FJ950474 / 1,1 / FJ950230 / FJ950231 / 38 8.807S,177 30.098E
L. hamiltoni / Maud Island / 2222 / FJ950426 / X86275 / FJ950321
L. archeyi / Whareorino / 104 / FJ950427 / FJ950532 / FJ950320 / 38 24.707S,174 41.490E