Supplementary Table 1. 16S rRNA gene clones obtained from OMW-mastoidis: closest phylogenetic relatives and their respective habitats.

Clones / Number
of clones / GenBank numbers / Closest phylogenetic relative / Similarity
(%) / Habitat-
Ecological niche / References-
Accession numbers

-Proteobacteria

MS-7, -59, -88, -113, -117, -129, -131, -136, -183, -219, -220, -225, -226, -232, -236, -240 & -252 / 17 / GQ477808-GQ477824 /

clone J20/

clone AV_4S-K23/
Acetobacter aceti / 97.6-99.7%/
97.1-97.7%/
97.0-98.0% / anaerobic sludge/
aircraft cabin air/
vinegar, alcoholic beverages etc / DQ168837/
EU341169/
Kersters et al.2006
MS-137, -138 & -218 / 3 / GQ477825-GQ477827 / “Ameyamaea chiangmaiensis”/
Acetobacter aceti / 97.3-98%/
96.0-97.0% / flower of red ginger, Thailand/
vinegar, alcoholic beverages etc / AB303367/
Kersters et al.2006
MS-123, -237 & -250 / 3 / GQ477828-GQ477830 / Acetobacter orientalis / 99.4-99.6% / fruits & fermented foods, Indonesia / Lisdiyanti et al.2001
MS-118 / 1 / GQ477831 / Acetobacter peroxydans / 99.1% / ditch water / Kersters et al.2006
MS-139 / 1 / GQ477832 / Acetobacter pasteurianus/
“Ameyamaea chiangmaiensis” / 95.3%/
95.1% / vinegar, beverages, fermented foods/
flower of red ginger, Thailand / Kersters et al.2006/
AB303367
MS-247 / 1 / GQ477833 / clone NCH1312/NCH31/
Acetobacter senegalensis*/
Acetobacter pasteurianus / 94.2%/
92.2%/
91.1% / instinal -longicorn beetle/
mango fruit, Senegal/
vinegar, beverages, fermented foods / EU560828/
AY883036/
Kersters et al.2006
MS-145 / 1 / GQ477834 / Acetobacter syzygii/
Acetobacter ghanensis / 99.1%/
98.8% / flowers and fruits, Indonesia/
cocoa-bean heap fermentation, Ghana / Lisdiyanti et al.2001/
EF030713
MS-16, -34, -51, -85, -122, -142, -182, -203, -204 & -228 / 10 / GQ477835-GQ477844 / Acetobacter tropicalis / 98.7-99.8% / fruits, Indonesia / Lisdiyanti et al.2000
MS-215, -216 & -248 / 3 / GQ477845-GQ477847 / Gluconacetobacter liquefaciens / 99.3-100% / sugar rich biotopes / Kersters et al.2006
MS-1, -8, -28 & -86 / 4 / GQ477848-GQ477851 / Gluconacetobacter liquefaciens/
Gluconacetobacter sacchari / 98.1-98.9%/
98.4-98.9% / sugar rich biotopes/
sugar cane -pink sugar-cane mealy bug / Kersters et al.2006/
AF127407
MS-115 / 1 / GQ477852 / strain CC-88226/
Gluconacetobacter sacchari/
Gluconacetobacter diazotrophicus / 99.5%/
99.0%/
98.8% / NR/
sugar cane -pink sugar-cane mealy bug/
sugar cane, coffee & sweet potato / AY961985/
AF127407/
Kersters et al.2006
MS-82 / 1 / GQ477853 / isolate Y57/
isolate P610-1/
Sphingomonas oligophenolica / 94.7%/
94.0%/
93.7% / degrading strain/
pollutant degrading strain/
paddy soil -phenolic acids degrader / DQ092868/
EF025345/
AB018439
-Proteobacteria
MS-102 / 1 / GQ477854 / clone RS62/
isolate Da-11/
Rubrivivax gelatinosus / 93.1%/
93.0%/
92.7% / Brackish water-coastal lagoon/
gut of Dermolepida alborhirtum/
freshwater, sewage, activated sludge / DQ450189/
EU073950/
Imhoff, 2005
-Proteobacteria
MS-35 / 1 / GQ477855 / Azomonas macrocytogenes / 96.9% / nitrogen-fixing bacterium / EF100146
Bacteroidetes
MS-38, -44, -52, -80, -81, -90, -96, -120, -130, -132, -163, -168, -185, -212, -214, -221, -227, -230, -239, -242, - 245 & -249 / 22 / GQ477856-GQ477877 / clone J5/
clone 9B/
Prevotella copri / 94.1-92.9%/
94.0-92.7%/
86.6-87.5% / anaerobic sludge/
predigester slurry, India/
human faeces / DQ168827/
EU887831/
AB064923
MS-29, -31, -33, -41 & -231 / 5 / GQ477878-GQ477882 / clone MumMBB04/
clone J5/
clone 9B/
Prevotella copri / 93.8-95.7%/
87.4-89.3 %/
87.3-89.2%/
87.5-88.8% / Atacameno mummy muscle/
anaerobic sludge/
predigester slurry, India/
human faeces / FJ793721/
DQ168827/
EU887831/
AB064923
Firmicutes
MS-25, -26, -30, -32, -36, -40, -45, -114, -116, -119, -121, -162, -224, -234, -238 & -243 / 16 / GQ477883-GQ477898 / Lactobacillus pentosus/
Lactobacillus plantarum / 98.2-99.4%/
98.1-99.4% / silage, fermented olives & sewage/
diary products, fermented foods, intestinal tract & sewage / Hammes and Hertel 2006/
Hammes and Hertel 2006
MS-125 & MS-244 / 2 / GQ477899-GQ477900 / Lactobacillus camelliae / 95.5-96.1% / fermented tea leaves, Thailand / AB257864
MS-79 & MS-222 / 2 / GQ477901-GQ477902 / clone ECH_aai37a02/
clone B416/
Lactobacillus rhamnosus / 96.8-94.2%/
93.5-94.8%/
93.5-94.7% / short-beaked echidna feces/
healthy human stool/
human saliva, clinical etc / EU460721/
DQ325752/
Hammes and Hertel 2006
MS-211 / 1 / GQ477903 / Oenococcus kitaharae / 99.6% / composting distilled shochu mashes / AB221475
MS-84 / 1 / GQ477904 / clone G35_D8_L_B_A11/
Eubacterium contortum/
Clostridium populeti / 94.5%/
92.0%/
91.9% / mesophilic anaerobic digester/
mouth & intestinal tract/
woody-biomass digestor / EF559143/
Wade 2006/
Sleat and Mah 1985
MS-146 & MS-198 / 2 / GQ477905-GQ477906 / Clostridium tyrobutyricum / 99.7-100% / fermentation, cheese, silage etc / Cato et al.1986
MS-148 / 1 / GQ477907 / clone SJTU_G_10_77/
clone A31/
Dialister succinatiphilus / 99.6%/
99.1%/
95.6% / human faeces/
anaerobic digester, China/
human faeces / EF405492/
EU234120/
AB370249
MS-197 / 1 / GQ477908 / strain NML 00-0124/
Ruminococcus albus / 90.7%/
85.4% / blood culture/
rumen / EU815223/
Bryant, 1986

Bold-type case indicates similarity in 16S rRNA gene below 97% with validly-described species of Bacteria. NR, not reported.*, 1.3% of the nucleotides in 16S rRNA gene were unidentified.

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