Supplementary Table 1: Mass Spectroscopy Data of Selected Proteins Identified in the -Carotene

Supplementary Table 1: Mass spectroscopy data of selected proteins identified in the -carotene (crtB) enhanced Camelina seeds with/without the SSP 2S suppression (RNAi2S/crtB). Data is presented by relative amounts of crtB proteins compared to nontransgenic, RNAi2S/crtB compared to nontransgenic and RNAi2S/crtB compared to crtB proteome. For full dataset see Suppl Table 2 and dataset identifier PXD002330 and 10.6019/PXD002330. The targeted 2S albumin SSP is severely reduced in the RNAi2S/crtB seeds while the other SSP, cruciferin, is notably increased during the seed protein rebalancing.

Accession / Protein name / crtB/
nontransgenic / RNAi2S/crtB / nontransgenic / RNAi2S/crtB/
crtB
NP_194581.1 / SSP cruciferin 3 / 0.89 / 1360.74 / 1513.36
NP_001078459.1 / SSP cruciferin 3 / 0.89 / 288.98 / 321.01
NP_849464.1 / SSP cruciferin 3 / 0.89 / 288.98 / 321.40
NP_849465.1 / SSP cruciferin 3 / 0.89 / 1107.94 / 1232.21
NP_171885.1 / SSP 12S / 0.70 / 3.20 / 2.26
NP_199225.1 / SSP 12S / 0.84 / 1.06 / 1.25
NP_194444.1 / SSP albumin 1 / 1.09 / 0.35 / 0.32
NP_194445.1 / SSP albumin 2 / 0.82 / 0.30 / 0.37
NP_194446.1 / SSP albumin 3 / 0.72 / 0.27 / 0.38
NP_001078112.1 / 60S ribosomal / 557.28 / 516.22 / 0.92
NP_177417.1 / Eukaryotic initiation factor 4a-3 / 492.82 / 612.01 / 1.24
NP_171974.1 / tubulin alpha 4 chain / 679.93 / 0.76 / 0.00
NP_175423.1 / tubulin alpha 2 chain / 678.41 / 0.76 / 0.00
NP_175945.1 / Chaperonin 60 subunit beta 1 / 503.19 / 547.03 / 1.08
NP_850563.1 / Universal stress protein-like protein / 638.36 / 0.76 / 0.00
NP_195235.1 / catalase 2 / 0.89 / 588.90 / 654.95
NP_001077564.1 / Dehydroascorbate reductase / 0.89 / 748.35 / 832.28
NP_176768.1 / NADP+ dependent isocitrate dehydrogenase / 0.89 / 604.71 / 672.53
NP_171936.1 / Malate dehydrogenase / 0.89 / 427.04 / 474.93
NP_173386.1 / Glutathione S-transferase like protein / 0.89 / 732.88 / 815.08
NP_190861.1 / Fructose bisphosphate aldolase / 0.89 / 1286.50 / 1430.80
NP_188144.1 / Putative protein phosphatase 2C 39 / 0.89 / 723.08 / 804.18
NP_194150.1 / HSP90-like protein GRP94 / 0.89 / 615.53 / 684.57