Warwick Medical School

2ndInternational Symposium on

Advances in Human Metabolism Research

PROGRAMME

Thursday 16MAY 2013

Society for Endocrinology Seminar

‘Metabolism, Mitochondria and Fatty Acids’

08.30 REGISTRATION, COFFEE, EXHIBITION

ChairProfessor Jonathan Arch

Clore Laboratories, University of Buckingham, UK

09.15Welcome

Professor Sudhesh Kumar, Warwick Medical School, UK

09.30Plenary Session: Uncoupling proteins

Professor David Nicholls, Buck Institute for Research on Aging, USA

10.00Fatty acid-induced defects in the bioenergetics of INS-1 insulinoma and skeletal muscle cells

Dr Charles Affourtit, Plymouth University, UK

10.30Studying mitochondrial morphology and dynamics

Professor OrianShirihai, Boston University School of Medicine, USA

11.00 COFFEE, POSTER SESSION, EXHIBITION

ChairDr John Clapham

Warwick Medical School, UK

11.45Elucidation of the routes of triglyceride synthesis: implications for metabolic homeostasis

Professor Victor Zammit, Warwick Medical School, UK

12.10Regulation of lipogenesis in developing adipocytes

Dr Justin Rochford, Rowett Institute of Nutrition and Health,

University of Aberdeen, UK

12.40CIDEA-dependent lipid droplet enlargement: how cells get fat

Dr Mark Christian, Warwick Medical School, UK

13.10 LUNCH, POSTER SESSION, EXHIBITION

ChairProfessor Charles Hutchinson

Warwick Medical School & University Hospitals Coventry and Warwickshire, UK

14.40Quantifying brown adipose tissue in human adults

Dr Tom Barber, Warwick Medical School & University Hospitals Coventry

and Warwickshire, UK

15.00IDEAL-MRI for the imaging of brown adipose tissue in human adults

Professor Charles Hutchinson, Warwick Medical School & University Hospitals Coventry and Warwickshire, UK

15.20 MRI of pericardial fat and heart function: (i) Newly refined ventricle-

specific analyses and (ii) imaging pre-and post-bariatric subjects

Professor James A Hamilton, Boston University School of Medicine, USA

15.50 Closing remarks

Professor Victor Zammit, Warwick Medical School, UK

16.00 - 18.00 CHEESE & WINE, POSTER PRIZES, EXHIBITION

19.30 Official Dinner(for registered delegates only)

Friday 17 MAY 2013

Training Workshop: ‘Energetics: from eukaryotes to man’

(for workshop registered delegates only)

8.00REGISTRATION, COFFEE

8.15Welcome

Professor Victor Zammit, Warwick Medical School, UK

8.30Mitochondrial diagnosis

Professor OrianShirihai, Boston University School of Medicine, USA

9.15Cellular Bionergetics

Professor David Nicholls, Buck Institute for Research on Aging, USA

10.00Probing the insulin sensitivity of skeletal muscle cells with Seahorse extra cellular flux technology

Dr Charles Affourtit, Plymouth University, UK

10.50SHUTTLE BUS DEPARTS TO UNIVERSITY HOSPITAL, COVENTRY

11.15Introduction to the XFe Wave Software

Experimental design for intact cells, isolated mitochondria and permeabilized cells using the Seahorse XF Stress test kits and reagents

Mr Alex Liversage, Seahorse Bioscience, UK

12.00PRACTICAL SESSION: Running an experiment using adherent cells on the XF24 analyser

13.00LUNCH

13.40Tour of HMRU (optional)

Dr Tom Barber, Warwick Medical School & University Hospitals Coventry and Warwickshire, UK

14.00Assay Optimizations – Key items to ensure XF assay success

Dr Alex Liversage, Seahorse Bioscience, UK

15.30 COFFEE and QUESTIONNAIRE

16.00One-on-one session: Consulting participants about their specific needs for research projects

17.00 Closing remarks

Professor Victor Zammit, Warwick Medical School, UK

17.50SHUTTLE BUS DEPARTS TO SCARMAN HOUSE, COVENTRY