Atxi-A LLKCVSKNIG FSHGKPVAAL TIYKCLMRWK IFEAEKTSIF DRIVPVFGSA

Supplemental Figure 1. Multiple sequence alignment of myosin XI C-terminal regions. Sequences homologous to amino acid residues 1109-1512 of myosin XI-2 from N. benthamiana were aligned in Clustal W 2.0 (Larkin et al., 2007) and gaps (other than one-nucleotide gaps) were removed manually to produce a more conservative alignment. Only sequences that began and ended with conserved regions were accepted. The alignment in Phylip format is given below. The sequences were AtXI-A (arabidopsis XI-A, AT1G04600.1), AtXI-D (arabidopsis XI-D, AT2G33240.1), AtXI-H (arabidopsis XI-H, AT4G28710.1), AtXi-G (arabidopsis XI-G, AT2G20290.1), AtXI-B (arabidopsis XI-B, AT1G04160.1), AtMya2 (arabidopsis MYA2, AT5G43900.1), NbXI-2 (N. benthamiana myosin XI-2, DQ875135), Nt175 (N. tabacum Ntmy175, AB082121) and AtXI-I (arabidopsis XI-I, AT4G33200.1).

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AtXI-A LLKCVSKNIG FSHGKPVAAL TIYKCLMRWK IFEAEKTSIF DRIVPVFGSA

AtXI-D LLKCVSKNVG FSHGKPVAAF TIYKCLIHWK LFEAEKTSVF DRIVPIFGSA

AtXI-H LINCVVNNIG FNQGKPVAAF TIYKCLLHWK SFEAERTSVF DRLVQMIGSA

AtXI-G LINSVTKNVG FSQGKPVAAF TIYKCLLHWK SFEAERTNVF DRLVQMIGSA

AtXI-B LIDCVKENIG FSNGKPIAAF TIYKCLLHWK CFESEKTSAF DRLIEMIGSA

AtMya2 LIDCVKDNIG FSNGKPVAAF TIYKCLLHWK CFESEKTNVF DRLIQMIGSA

NbXI-2 LIDCVMKDVG FSQGKPVAAF TIYKCLLNWK SFEAERTSVF DRLIQMIGSA

Nt175 LIDCVMKDVG FSQGKPVAAF TIYKCLLNWK SFEAERTSVF DRLIQMIGSA

AtXI-I LSRCIKENLG FNDDKPLAAC VIYKCLLHWR AFESESTAIF NIIIEGINEA

IENQEDDNHL AYWLTNTSTL LFLLQRSLRV DARYPALLFK QQLTAYVETM

IENPEDDSNL AYWLTNTSTL LFLLQRSLKV DARYPALLFK QQLTAYIETI

IKDEGDNEHL AYWLSNTSTL LFMIQQSLKV VAKDPALLFK QQLTAYVEKI

IKDEDNDANL AYWLSNTSTL LFMLQQSLKV DAKDPALHFK QQLEAYVEKI

IENEDDNGHL AYWLTNTSAL LFLLQKSLKV EAKYPALLFK QQLAAYVEKI

IENEDDNSHL AYWLTSTSAL LFLLQKSLKV EAKYPALLFK QQLAAYVEKM

IENQESNDHM AYWLSNTSTL LFLIQKSLKV EAKYPALLFK QQLTAYVEKI

LKGGDENGVL PYWLSNASAL LCLLQRNLRI EARYPALLFK QQLTACVEKI

YGIIRENVKR EVSSLLSSCI QWQSIIEFLN YILITWKKNY VPLFLVQKMF

YGIFQENVKR KLAPVLSSCI QWQDVIGLLN QLLGTLKKNY VPLFLAQKIF

FGMIRDNLKN ELQTLLSLCI QWNGIYDGLN AILSTLQENF VPPVLIQNIF

LGIIWDNLKK ELNTVLALCI QWQDIIEGLD ALLSTLKESF VPPVLIQKIF

FGMIRDNLKK ELSALISMCI QWQSIIDGLN SLLAILKDNY VPLVLIQKIH

FGMVRDNLKR ELSTLLSLCI QWQSIIDGLN SLLVTLKENH VPLVLIQKIY

YGIIRDNLKK ELGSLLSLCI QWQRIIECLN SLLCTLKENF VPPILVQKIF

YGLIRDNLKK ELSPLLGSCI QWESILKFLD SLMSRLRENH VPSFFIRKLV

SQTFQYINVQ LFNSLLLERE YCTVNMGIKV KAGLDELESW CSQATEEFVG

CQTFQDINVQ LFNS-LLQRE CCTFIMGKKV NVWLNELESW CSQATEDFVG

IQTFSFINVQ LFNSLLLRRE CCTFSNGEFV KSGLALLEEW CNETTEEYAG

SQAFSLINVQ VCNSLVTRPD NCSFINGEYL KSGLEKLEKW CCETKEEYAG

TQTFSFVNVQ LFNSLLLRKE CCTFSNGEFV KSGLAELELW CGQVN-EYAG

SQTFSYINVQ LFNSLLLRKE CCTFSNGEFV KSGLAELELW CCQAK-EYSG

TQTFSYINVQ LFNSLLLRRE CCTFSNGEYV KAGLAELELW CCQAKEEYAG

TQVFSFINLS LFNSLLLRRE CCTFSNGEYV KSGISELEKW IANAKEEFAG

SSWDELKHTR QAVVLLVTEP KSTITYDDLT INLCSVLSTE QLYRICTLCK

SSWDELKNTR QALVLLVTEQ KSTITYDDLT TNLCPALSTQ QLYRICTLCK

SSWDELKHIR QAVGFMVIHK KYRISYDDIA HDLCPILSVQ QLYRICTLYW

SSWDELKHTR QAVGFLLIHK KYNISYDEIA NDLCPNLQIQ QHFKLCTLYK

PSWDELKHIR QAVGFLVIHQ KYRVSYDDIV HDLCPILSVQ QLYRICTLYW

PSWEELKHIR QAVGFLVIHQ KYRISYDEIA NDLCPVLSVQ QLYRICTLYW

SSWDELKHIR QAVGFLVIHQ KYRISYDEIT NDLCPILSVQ QLYRICTLYW

SSWDELKHIR QAVGFLVIHQ KYRISYDEIT NDLCPILSVQ QLYRICTLYG

TSWHELNYIR QAVGFLVIHQ KKKKSLDEIR QDLCPVLTIR QIYRISTMYW

DKDDGDHNVS PEVISNLKLL LSRSFLLDDD SSIPFDTDEI SSCMQEKDFA

IDDHEDQNVS PDVISNLKLL VSRSFLLDNN SSIPFAADEI SNSMQEKDFT

DDSYNTRSVS QDVIANMRVL MSSAFLLDED SSIPFSADDL SSSMKEKDFA

DEIYNTKSVS QDVIASMTGV MSSDFLLKED SSISLSIDDL CSSMQDKDFA

DDCYNTRSVS QEVISSMRAL MSNSFLLDDN SSIPFSIDEI SNSMHEKDFA

DDSYNTRSVS QEVISSMRTL MSDSFLLDDD SSIPFSIDDI SSSMEEKDFV

DDNYNTRSVS PDVISSMRVL MSNSFLLDDN SSIPFSIDEV SESLQVKDFA

DDKYGTQSVS SEVVSQMRVL VSNSFLLDDD MSIPFSAEDI DKAIPVLDPS

NVKASELADN PNF

NVKAVELADN PNF